BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0066.p (330 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB22.1 B3KB22_9BACI Ketol-acid reductoisomerase EC=1.1.1.86 641 0.0 B1DL47.1 B1DL47_9BACL Ketol-acid reductoisomerase EC=1.1.1.86 580 e-164 C0Z9C7.1 ILVC_BREBN Ketol-acid reductoisomerase EC=1.1.1.86 509 e-142 C3B272.1 C3B272_BACMY Ketol-acid reductoisomerase 497 e-139 C3AU22.1 C3AU22_BACMY Ketol-acid reductoisomerase 497 e-139 >B3KB22.1 B3KB22_9BACI Ketol-acid reductoisomerase EC=1.1.1.86 Length = 330 Score = 641 bits (1654), Expect = 0.0, Method: Compositional matrix adjust. Identities = 303/330 (91%), Positives = 323/330 (97%) Query: 1 MAVTTYYEQDAELSVLKGKTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSFETAKND 60 MA+TTYYEQDAELSVLK KTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSF+ AKND Sbjct: 1 MAITTYYEQDAELSVLKDKTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSFDKAKND 60 Query: 61 GFEVLPVAEAVSRADVVQILMPDETQASVYKNEIEPNLKNGAALMFSHGFNVHFGQIVAP 120 GFEVLPVAEAVSRADVVQILMPDETQA+VYKN+IEPNLK GAALMFSHGFNVHFGQIVAP Sbjct: 61 GFEVLPVAEAVSRADVVQILMPDETQAAVYKNDIEPNLKKGAALMFSHGFNVHFGQIVAP 120 Query: 121 KDADVLLVAPKSPGHMVRRTYVEGFGVPGLIAIEQNATGNAQAIGLAYAKGIGCTRAGVI 180 KD DVLLVAPKSPGHMVRRTYVEGFGVPGLIAIE++ATG A+AIGLAYAKGIGCTRAGVI Sbjct: 121 KDNDVLLVAPKSPGHMVRRTYVEGFGVPGLIAIEKDATGQAKAIGLAYAKGIGCTRAGVI 180 Query: 181 ETSFREETETDLFGEQAVLCGGVSALIKAGFETLTEAGYAPEMAYFECLHEMKLIVDLVY 240 ETSFREETETDLFGEQAVLCGGVSAL+KAGFETLTEAGYAPEMAYFECLHE+KLIVD++Y Sbjct: 181 ETSFREETETDLFGEQAVLCGGVSALVKAGFETLTEAGYAPEMAYFECLHELKLIVDMIY 240 Query: 241 EGGLATMRDSISNTAEYGDYVTGPRIITDETKKAMKAVLSDIQQGKFARDFILENQSGRA 300 EGGLA+MRDSISNTAEYGDYVTGPR++T++TKKAMK VLSDIQQGKFARDFILENQSGRA Sbjct: 241 EGGLASMRDSISNTAEYGDYVTGPRVVTEDTKKAMKEVLSDIQQGKFARDFILENQSGRA 300 Query: 301 FLTATRRNEAAHPVEVVGSQLREMMHWIKK 330 FLTATRRNE+ HP+EVVG++LREMMHWIKK Sbjct: 301 FLTATRRNESEHPIEVVGAELREMMHWIKK 330 >B1DL47.1 B1DL47_9BACL Ketol-acid reductoisomerase EC=1.1.1.86 Length = 330 Score = 580 bits (1496), Expect = e-164, Method: Compositional matrix adjust. Identities = 277/330 (83%), Positives = 297/330 (90%) Query: 1 MAVTTYYEQDAELSVLKGKTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSFETAKND 60 MAVT YYE+DA+ VL+GKTIAVIGYGSQGHAQAQNLRDSGL+VVIGLR GKS + A D Sbjct: 1 MAVTLYYEKDADQGVLQGKTIAVIGYGSQGHAQAQNLRDSGLKVVIGLRPGKSADVAAKD 60 Query: 61 GFEVLPVAEAVSRADVVQILMPDETQASVYKNEIEPNLKNGAALMFSHGFNVHFGQIVAP 120 GFEVL VAEA ADVVQIL+PDETQA VY EIEPNLK GAALMFSHGFN+H+GQIV Sbjct: 61 GFEVLTVAEATKVADVVQILLPDETQAKVYAEEIEPNLKKGAALMFSHGFNIHYGQIVPS 120 Query: 121 KDADVLLVAPKSPGHMVRRTYVEGFGVPGLIAIEQNATGNAQAIGLAYAKGIGCTRAGVI 180 KD DV LVAPKSPGHMVRRTY EGFGVPGLIAI Q+A+GNA+AIGLAYAKGIGCTRAGVI Sbjct: 121 KDTDVFLVAPKSPGHMVRRTYQEGFGVPGLIAIHQDASGNAKAIGLAYAKGIGCTRAGVI 180 Query: 181 ETSFREETETDLFGEQAVLCGGVSALIKAGFETLTEAGYAPEMAYFECLHEMKLIVDLVY 240 ETSF+EETETDLFGEQAVLCGG SAL+KAGFETL EAGYAPEMAYFECLHE+KLIVDL+Y Sbjct: 181 ETSFKEETETDLFGEQAVLCGGASALVKAGFETLVEAGYAPEMAYFECLHELKLIVDLMY 240 Query: 241 EGGLATMRDSISNTAEYGDYVTGPRIITDETKKAMKAVLSDIQQGKFARDFILENQSGRA 300 EGGLATMRDSISNTAEYGDYVTGPRI+T+ETKK MK VL DIQ G+FARDFILENQS RA Sbjct: 241 EGGLATMRDSISNTAEYGDYVTGPRIVTEETKKEMKRVLEDIQSGRFARDFILENQSNRA 300 Query: 301 FLTATRRNEAAHPVEVVGSQLREMMHWIKK 330 LTATRRNEAAHP+E G QLRE+MHWIKK Sbjct: 301 MLTATRRNEAAHPIEQTGKQLRELMHWIKK 330 >C0Z9C7.1 ILVC_BREBN Ketol-acid reductoisomerase EC=1.1.1.86 Length = 340 Score = 509 bits (1310), Expect = e-142, Method: Compositional matrix adjust. Identities = 241/328 (73%), Positives = 276/328 (84%) Query: 3 VTTYYEQDAELSVLKGKTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSFETAKNDGF 62 V YYE D + VL+GKTIA+IGYGSQGHAQAQNLRDSG +VV+GLR GKS++ A DGF Sbjct: 2 VKMYYEADVKQEVLRGKTIAIIGYGSQGHAQAQNLRDSGYKVVVGLRPGKSWDVAAKDGF 61 Query: 63 EVLPVAEAVSRADVVQILMPDETQASVYKNEIEPNLKNGAALMFSHGFNVHFGQIVAPKD 122 EVL VAEA RADVVQILMPDE QA VY++EI PNLK+GAAL FSHGFN+H+ QIV P D Sbjct: 62 EVLTVAEATKRADVVQILMPDERQAQVYRDEIAPNLKSGAALCFSHGFNIHYAQIVPPAD 121 Query: 123 ADVLLVAPKSPGHMVRRTYVEGFGVPGLIAIEQNATGNAQAIGLAYAKGIGCTRAGVIET 182 DV++ APKSPGH+VRR Y EGFGVPGLIAI Q+ATGNA+ + LAY+ GIGCT+AGVIET Sbjct: 122 VDVIMAAPKSPGHLVRRVYQEGFGVPGLIAIYQDATGNAKDLALAYSSGIGCTKAGVIET 181 Query: 183 SFREETETDLFGEQAVLCGGVSALIKAGFETLTEAGYAPEMAYFECLHEMKLIVDLVYEG 242 SFREETETDLFGEQAVLCGG S L+KAGF+TL EAGYAPE+AYFECLHE+KLIVDL+YEG Sbjct: 182 SFREETETDLFGEQAVLCGGASELVKAGFDTLVEAGYAPEIAYFECLHELKLIVDLMYEG 241 Query: 243 GLATMRDSISNTAEYGDYVTGPRIITDETKKAMKAVLSDIQQGKFARDFILENQSGRAFL 302 GLA MR SIS+TAEYGDY TG RIITDET+K MK VL++IQ G FAR++ILENQ+ R Sbjct: 242 GLARMRYSISDTAEYGDYSTGRRIITDETRKEMKKVLAEIQDGTFARNWILENQANRPGF 301 Query: 303 TATRRNEAAHPVEVVGSQLREMMHWIKK 330 T+ RR E+ H +EVVG QLR MM WI K Sbjct: 302 TSRRRLESEHGIEVVGEQLRGMMSWINK 329 >C3B272.1 C3B272_BACMY Ketol-acid reductoisomerase Length = 341 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 231/328 (70%), Positives = 276/328 (84%) Query: 3 VTTYYEQDAELSVLKGKTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSFETAKNDGF 62 + TYYE+DA L VL GKT+AV+GYGSQGHAQAQNLRDSG+ VV+G+R GKSF AK DGF Sbjct: 5 MKTYYEKDANLEVLAGKTVAVVGYGSQGHAQAQNLRDSGVHVVVGVRPGKSFSVAKADGF 64 Query: 63 EVLPVAEAVSRADVVQILMPDETQASVYKNEIEPNLKNGAALMFSHGFNVHFGQIVAPKD 122 EV+ V+EAV ADVVQ+L+PDE QA VY+NE+E NL+ G L+FSHGFN+HFGQI P Sbjct: 65 EVMSVSEAVRTADVVQVLLPDEQQAHVYRNEVEKNLREGQMLLFSHGFNIHFGQIQPPSY 124 Query: 123 ADVLLVAPKSPGHMVRRTYVEGFGVPGLIAIEQNATGNAQAIGLAYAKGIGCTRAGVIET 182 DV +VAPKSPGH+VRR + EG GVP L+A+ QNATGNA + LAYAKG+GCTRAGVIET Sbjct: 125 VDVAMVAPKSPGHLVRRVFQEGNGVPALVAVYQNATGNAFDVALAYAKGVGCTRAGVIET 184 Query: 183 SFREETETDLFGEQAVLCGGVSALIKAGFETLTEAGYAPEMAYFECLHEMKLIVDLVYEG 242 SF+EETETDLFGEQAVLCGGV+AL+KAGFETLTE GY PE+AYFECLHE+KLIVDL+YEG Sbjct: 185 SFQEETETDLFGEQAVLCGGVTALVKAGFETLTEGGYRPEIAYFECLHELKLIVDLMYEG 244 Query: 243 GLATMRDSISNTAEYGDYVTGPRIITDETKKAMKAVLSDIQQGKFARDFILENQSGRAFL 302 GL MR SIS+TAE+GDYVTG RI+TDETKK MK VL++IQQG+FA+ +ILENQ+GR Sbjct: 245 GLTNMRHSISDTAEFGDYVTGSRIVTDETKKEMKRVLAEIQQGEFAKKWILENQAGRPTY 304 Query: 303 TATRRNEAAHPVEVVGSQLREMMHWIKK 330 A ++ E H +E VG++LREMM WI++ Sbjct: 305 NAMKKAEQNHQLEKVGAELREMMTWIQQ 332 >C3AU22.1 C3AU22_BACMY Ketol-acid reductoisomerase Length = 341 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 231/328 (70%), Positives = 276/328 (84%) Query: 3 VTTYYEQDAELSVLKGKTIAVIGYGSQGHAQAQNLRDSGLQVVIGLREGKSFETAKNDGF 62 + TYYE+DA L VL GKT+AV+GYGSQGHAQAQNLRDSG+ VV+G+R GKSF AK DGF Sbjct: 5 MKTYYEKDANLEVLAGKTVAVVGYGSQGHAQAQNLRDSGVHVVVGVRPGKSFSVAKADGF 64 Query: 63 EVLPVAEAVSRADVVQILMPDETQASVYKNEIEPNLKNGAALMFSHGFNVHFGQIVAPKD 122 EV+ V+EAV ADVVQ+L+PDE QA VY+NE+E NL+ G L+FSHGFN+HFGQI P Sbjct: 65 EVMSVSEAVRTADVVQVLLPDEQQAHVYRNEVEKNLREGQMLLFSHGFNIHFGQIQPPSY 124 Query: 123 ADVLLVAPKSPGHMVRRTYVEGFGVPGLIAIEQNATGNAQAIGLAYAKGIGCTRAGVIET 182 DV +VAPKSPGH+VRR + EG GVP L+A+ QNATGNA + LAYAKG+GCTRAGVIET Sbjct: 125 VDVAMVAPKSPGHLVRRVFQEGNGVPALVAVYQNATGNAFDVALAYAKGVGCTRAGVIET 184 Query: 183 SFREETETDLFGEQAVLCGGVSALIKAGFETLTEAGYAPEMAYFECLHEMKLIVDLVYEG 242 SF+EETETDLFGEQAVLCGGV+AL+KAGFETLTE GY PE+AYFECLHE+KLIVDL+YEG Sbjct: 185 SFQEETETDLFGEQAVLCGGVTALVKAGFETLTEGGYRPEIAYFECLHELKLIVDLMYEG 244 Query: 243 GLATMRDSISNTAEYGDYVTGPRIITDETKKAMKAVLSDIQQGKFARDFILENQSGRAFL 302 GL MR SIS+TAE+GDYVTG RI+TDETKK MK VL++IQQG+FA+ +ILENQ+GR Sbjct: 245 GLTNMRHSISDTAEFGDYVTGSRIVTDETKKEMKRVLAEIQQGEFAKKWILENQAGRPTY 304 Query: 303 TATRRNEAAHPVEVVGSQLREMMHWIKK 330 A ++ E H +E VG++LREMM WI++ Sbjct: 305 NAMKKAEQNHQLEKVGAELREMMTWIQQ 332 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,865,934,308 Number of extensions: 75133935 Number of successful extensions: 222740 Number of sequences better than 1.0e-05: 1541 Number of HSP's gapped: 220359 Number of HSP's successfully gapped: 1791 Length of query: 330 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 194 Effective length of database: 1,779,438,163 Effective search space: 345211003622 Effective search space used: 345211003622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)