BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0056.p (180 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DEY4.1 B1DEY4_9BACL Thioesterase superfamily protein 223 4e-57 Q65KV8.1 Q65KV8_BACLD YkhA 221 2e-56 Q62WA5.1 Q62WA5_BACLD Putative acyl-CoA thioester hydrolase 221 2e-56 A8FCB1.1 A8FCB1_BACP2 Acyl-CoA thioester hydrolase 220 3e-56 B4AEX6.1 B4AEX6_BACPU YkhA 218 3e-55 >B1DEY4.1 B1DEY4_9BACL Thioesterase superfamily protein Length = 170 Score = 223 bits (569), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 104/167 (62%), Positives = 131/167 (78%) Query: 14 KYCRESRVFKTSRVFPNDVNNHKTLFGGKLMSTIDEVASISAMRHCRTNVVTASADSVDF 73 ++ RESR +KTSR+FP DVNNH TLFGGKLM+ ID++ASI+A +HCR VVTAS DSVDF Sbjct: 4 RFSRESRCYKTSRIFPTDVNNHNTLFGGKLMAYIDDIASIAATKHCRRAVVTASTDSVDF 63 Query: 74 LLPIRPTDSVCFESFVSWTGRTSIEVFVKIISENLYTGERAVAATSFLTFVAVHEDGTPA 133 L PIRP+DSVC ESFV+WTGRTS+EVFVK+I E+L TGER VAATSFLTFVA+ ++ P Sbjct: 64 LQPIRPSDSVCLESFVTWTGRTSVEVFVKVIKEDLLTGERIVAATSFLTFVALDQNRHPV 123 Query: 134 PVPAVVAETDEEKLISASADQRSELRKIRRTSSKLLASQLSTTKYWE 180 PVP V+ E++EE + +A R+E+R+IRR SK A L+ W+ Sbjct: 124 PVPKVIPESEEEFKLYETASHRAEMRRIRREESKKFADYLTVAHPWD 170 >Q65KV8.1 Q65KV8_BACLD YkhA Length = 173 Score = 221 bits (564), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 106/170 (62%), Positives = 128/170 (75%) Query: 10 APDFKYCRESRVFKTSRVFPNDVNNHKTLFGGKLMSTIDEVASISAMRHCRTNVVTASAD 69 P+ +YC+ES+V KTSRVFP D NNH TLFGGKLMS ID++ASISA RH R VTAS D Sbjct: 4 GPEKRYCKESKVVKTSRVFPLDTNNHNTLFGGKLMSYIDDIASISAARHSRCETVTASMD 63 Query: 70 SVDFLLPIRPTDSVCFESFVSWTGRTSIEVFVKIISENLYTGERAVAATSFLTFVAVHED 129 SVDFL PIR DSVC ES+V+W GRTS+EVFVK+I E+L TGER +AATSFLTFVA+ +D Sbjct: 64 SVDFLEPIRQEDSVCLESYVTWVGRTSMEVFVKVIKEHLLTGERKLAATSFLTFVALDQD 123 Query: 130 GTPAPVPAVVAETDEEKLISASADQRSELRKIRRTSSKLLASQLSTTKYW 179 G P VP VV +++EE ++ +A QR+E RK R SK LA L+ K W Sbjct: 124 GNPQAVPQVVPQSEEEIMLHETARQRAEERKNHRKHSKALAQALAVDKPW 173 >Q62WA5.1 Q62WA5_BACLD Putative acyl-CoA thioester hydrolase Length = 172 Score = 221 bits (563), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 106/170 (62%), Positives = 128/170 (75%) Query: 10 APDFKYCRESRVFKTSRVFPNDVNNHKTLFGGKLMSTIDEVASISAMRHCRTNVVTASAD 69 P+ +YC+ES+V KTSRVFP D NNH TLFGGKLMS ID++ASISA RH R VTAS D Sbjct: 3 GPEKRYCKESKVVKTSRVFPLDTNNHNTLFGGKLMSYIDDIASISAARHSRCETVTASMD 62 Query: 70 SVDFLLPIRPTDSVCFESFVSWTGRTSIEVFVKIISENLYTGERAVAATSFLTFVAVHED 129 SVDFL PIR DSVC ES+V+W GRTS+EVFVK+I E+L TGER +AATSFLTFVA+ +D Sbjct: 63 SVDFLEPIRQEDSVCLESYVTWVGRTSMEVFVKVIKEHLLTGERKLAATSFLTFVALDQD 122 Query: 130 GTPAPVPAVVAETDEEKLISASADQRSELRKIRRTSSKLLASQLSTTKYW 179 G P VP VV +++EE ++ +A QR+E RK R SK LA L+ K W Sbjct: 123 GNPQAVPQVVPQSEEEIMLHETARQRAEERKNHRKHSKALAQALAVDKPW 172 >A8FCB1.1 A8FCB1_BACP2 Acyl-CoA thioester hydrolase Length = 174 Score = 220 bits (561), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 105/168 (62%), Positives = 129/168 (76%) Query: 12 DFKYCRESRVFKTSRVFPNDVNNHKTLFGGKLMSTIDEVASISAMRHCRTNVVTASADSV 71 +FKYC+ES+V KTSRVFP D NNH+TLFGGKLMS ID++ASISA RH R+ VTAS DSV Sbjct: 6 NFKYCKESKVVKTSRVFPLDTNNHQTLFGGKLMSYIDDIASISAARHSRSETVTASMDSV 65 Query: 72 DFLLPIRPTDSVCFESFVSWTGRTSIEVFVKIISENLYTGERAVAATSFLTFVAVHEDGT 131 DFL PI +SVC ES+V+W G +S+EVFVK++ ENL +GER +AATSFLTFV++ EDG Sbjct: 66 DFLEPIGQKESVCLESYVTWVGTSSMEVFVKVMKENLMSGERRLAATSFLTFVSLDEDGK 125 Query: 132 PAPVPAVVAETDEEKLISASADQRSELRKIRRTSSKLLASQLSTTKYW 179 P VP V+ ET+EE ++ +A QR+E RK RR SK LA L T K W Sbjct: 126 PKRVPQVMPETEEEIMLHQTAKQRAEERKSRRKHSKALADALGTDKPW 173 >B4AEX6.1 B4AEX6_BACPU YkhA Length = 174 Score = 218 bits (554), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 104/168 (61%), Positives = 128/168 (76%) Query: 12 DFKYCRESRVFKTSRVFPNDVNNHKTLFGGKLMSTIDEVASISAMRHCRTNVVTASADSV 71 +FKYC+ES+V KTSRVFP D NNH+TLFGGKLMS ID++ASISA RH R VTAS DSV Sbjct: 6 NFKYCKESKVVKTSRVFPLDTNNHQTLFGGKLMSYIDDIASISAARHSRCETVTASMDSV 65 Query: 72 DFLLPIRPTDSVCFESFVSWTGRTSIEVFVKIISENLYTGERAVAATSFLTFVAVHEDGT 131 DFL PI +SVC ES+V+W G +S+EVFVK+I ENL +GER +AATSFLTFV++ ++G Sbjct: 66 DFLEPIGQKESVCLESYVTWVGTSSMEVFVKVIKENLMSGERRLAATSFLTFVSLDDNGK 125 Query: 132 PAPVPAVVAETDEEKLISASADQRSELRKIRRTSSKLLASQLSTTKYW 179 P VP V+ ET+EE ++ +A QR+E RK RR SK LA L T K W Sbjct: 126 PKKVPQVMPETEEEIMLHQTAKQRAEERKSRRKHSKALADALGTDKPW 173 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.128 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 937,795,962 Number of extensions: 33297267 Number of successful extensions: 93305 Number of sequences better than 1.0e-05: 1255 Number of HSP's gapped: 92966 Number of HSP's successfully gapped: 1294 Length of query: 180 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 53 Effective length of database: 1,864,227,298 Effective search space: 98804046794 Effective search space used: 98804046794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)