BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0050.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB12.1 B3KB12_9BACI Monogalactosyldiacylglycerol synthase 585 e-165 B1D5R4.1 B1D5R4_9BACL Monogalactosyldiacylglycerol synthase 441 e-122 B3KEA6.1 B3KEA6_9BACI Monogalactosyldiacylglycerol synthase 423 e-116 B8I3U5.1 B8I3U5_CLOCE Monogalactosyldiacylglycerol synthase 210 2e-52 A7FPG9.1 A7FPG9_CLOB1 Putative uncharacterized protein 204 1e-50 >B3KB12.1 B3KB12_9BACI Monogalactosyldiacylglycerol synthase Length = 375 Score = 585 bits (1507), Expect = e-165, Method: Compositional matrix adjust. Identities = 269/371 (72%), Positives = 321/371 (86%), Gaps = 2/371 (0%) Query: 5 LRKKRVLLLSEGFGAGHTQAAYALSSSLRKLSPNLQTKVLELGSFLNPRLAPLIVSAYRK 64 + KKRVLLLSEGFGAGHTQAA+ALSSSLR+LSP++QTKVLELGSFLNP++APLI+SAYRK Sbjct: 1 MAKKRVLLLSEGFGAGHTQAAHALSSSLRQLSPSVQTKVLELGSFLNPKIAPLIISAYRK 60 Query: 65 TVTSQPRLMGYVYRHQKSFNRLTTLALHRIFYTHTQNIMKQLKPDIIVCTHFIPSAVVSR 124 T+T++PRL+GY+YRHQKSFNR T LALHR+FYT T+N+++QL+PDIIVCTHFIPSAV+SR Sbjct: 61 TITTRPRLVGYMYRHQKSFNRFTALALHRMFYTSTKNVVRQLRPDIIVCTHFIPSAVISR 120 Query: 125 LKRLDPAFKVPLVTVITDYDAHATWISPEVDRYLVSTPEVKTKLRYRSVAAAKIQVTGMP 184 LKRL VPL TVITDYDAH TWISPEVDRY VSTPEVK KL R V+ AKIQVTG+P Sbjct: 121 LKRL--GTNVPLCTVITDYDAHDTWISPEVDRYFVSTPEVKRKLLNRGVSQAKIQVTGIP 178 Query: 185 VHPSFWEHPGKADILEQFGLQDMPTVLVMGGGWGMMNDEVINTALAGWRDKLQIIFCLGK 244 +HP FW HP K +I +F L DMPTVLVMGGGWG+MNDEV++ L WR+++QIIFCLG Sbjct: 179 IHPDFWTHPSKEEIRARFNLSDMPTVLVMGGGWGIMNDEVVHEFLTRWREEVQIIFCLGN 238 Query: 245 NEKLLREMQGNPLYSHPNISLVGFTREIDKLMEISDLLVTKPGGMTCSEGLAKGIPMLFY 304 N+K EM+ N + HPNI ++GFTREIDKLME+SDLLVTKPGGMTC+EGLAKGIPMLF+ Sbjct: 239 NDKGREEMESNSRFQHPNIRILGFTREIDKLMEVSDLLVTKPGGMTCTEGLAKGIPMLFH 298 Query: 305 DPLPGQEEENCRYFTAAGLGEPVTSLKVVDRWMERLLYSYDEVQAKRQSHLDEIARFHPL 364 PLPGQEEENC+YFTA GLGEP+ SL+V+ +WM +LL+ YD +Q +R +L +I RFHP+ Sbjct: 299 QPLPGQEEENCQYFTAQGLGEPIHSLEVLGKWMNKLLHQYDLIQEQRAQNLQDIERFHPM 358 Query: 365 QSAQSIIDMLE 375 QSA+SIIDMLE Sbjct: 359 QSARSIIDMLE 369 >B1D5R4.1 B1D5R4_9BACL Monogalactosyldiacylglycerol synthase Length = 371 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 199/375 (53%), Positives = 289/375 (77%), Gaps = 8/375 (2%) Query: 5 LRKKRVLLLSEGFGAGHTQAAYALSSSLRKLSPNLQTKVLELGSFLNPRLAPLIVSAYRK 64 +RKKRVLLLSEGFG+GHTQAA+AL++ LR+L+P++QT+V+ELG+FLNP L PLI+SAYRK Sbjct: 1 MRKKRVLLLSEGFGSGHTQAAHALAAGLRQLNPDIQTRVMELGNFLNPVLGPLIISAYRK 60 Query: 65 TVTSQPRLMGYVYR--HQKSFNRLTTLALHRIFYTHTQNIMKQLKPDIIVCTHFIPSAVV 122 TV+ QP+++G +YR ++KS NR LALHR+FYTHT ++ QLKPD+++CTH +P+AVV Sbjct: 61 TVSKQPKMVGLMYRSNYKKSLNRFMQLALHRMFYTHTSQVIAQLKPDLVICTHPVPNAVV 120 Query: 123 SRLKRLDPAFKVPLVTVITDYDAHATWISPEVDRYLVSTPEVKTKLRYRSVAAAKIQVTG 182 SRLKRL + L TVITDYDAH +W++ EV+++LVS+ VK KL SV ++I VTG Sbjct: 121 SRLKRL--GLNIKLCTVITDYDAHGSWVNQEVNKFLVSSSLVKDKLINHSVPESRIAVTG 178 Query: 183 MPVHPSFWEHPGKADILEQFGLQDMPTVLVMGGGWGMMNDEVINTALAGWRDKLQIIFCL 242 +PVHP FW+ K I EQFGL+++PTVL+MGGGWG+M ++ + + WR+++Q IFC+ Sbjct: 179 IPVHPLFWKSYDKVQIREQFGLKELPTVLIMGGGWGLMYEDSLLEYMTEWRERVQFIFCV 238 Query: 243 GKNEKLLREMQGNPLYSHPNISLVGFTREIDKLMEISDLLVTKPGGMTCSEGLAKGIPML 302 G N+K+ +M NP + H N+ ++GFT+E+ KLM+++D+LVTKPGGMTC+EG++KGIPML Sbjct: 239 GNNDKVKEKMLANPRFRHNNVRIIGFTKEVSKLMDVADVLVTKPGGMTCTEGMSKGIPML 298 Query: 303 FYDPLPGQEEENCRYFTAAGLGEPVTSLKVVDRWMERLLYSYDEVQAK-RQSHLDE-IAR 360 FY+P+PGQEE+NC YF G GE + + + VD+WM + E A+ RQ+ +++ Sbjct: 299 FYEPIPGQEEQNCEYFIKNGFGEMLETTETVDKWMA--VIQQPETSARYRQTLINKRNEE 356 Query: 361 FHPLQSAQSIIDMLE 375 ++PL ++++ +L+ Sbjct: 357 YNPLTCPKAVLQLLQ 371 >B3KEA6.1 B3KEA6_9BACI Monogalactosyldiacylglycerol synthase Length = 391 Score = 423 bits (1088), Expect = e-116, Method: Compositional matrix adjust. Identities = 193/351 (54%), Positives = 264/351 (75%), Gaps = 4/351 (1%) Query: 5 LRKKRVLLLSEGFGAGHTQAAYALSSSLRKLSPNLQTKVLELGSFLNPRLAPLIVSAYRK 64 +R RVLL SEGFG GHTQAAYAL+ +++++P + +V+ELG+FLNP + PLI+SAYRK Sbjct: 1 MRYTRVLLFSEGFGTGHTQAAYALAEGIQRMNPRIHCRVIELGNFLNPTVGPLILSAYRK 60 Query: 65 TVTSQPRLMGYVYRHQ--KSFNRLTTLALHRIFYTHTQNIMKQLKPDIIVCTHFIPSAVV 122 TV+++PRL+G +YR Q KS NRLT LALHRIFY Q ++++LKPD+I+CTH P+AVV Sbjct: 61 TVSTRPRLVGMLYRSQYKKSLNRLTRLALHRIFYAQAQRVIEELKPDLIICTHPFPNAVV 120 Query: 123 SRLKRLDPAFKVPLVTVITDYDAHATWISPEVDRYLVSTPEVKTKLRYRSVAAAKIQVTG 182 SRLKR VPL T+ITDYDAH TW++PEVD YLVSTP VK L R + I VTG Sbjct: 121 SRLKR--QGLDVPLYTLITDYDAHGTWVNPEVDEYLVSTPHVKKMLMLREIEPEFIHVTG 178 Query: 183 MPVHPSFWEHPGKADILEQFGLQDMPTVLVMGGGWGMMNDEVINTALAGWRDKLQIIFCL 242 +PVHP FWE + + + L++MPTVL+MGGGWG++ ++ + + LAG D +Q++FC+ Sbjct: 179 IPVHPKFWERGSRVALQAELELKNMPTVLIMGGGWGLVFNKELLSKLAGRADDIQLVFCM 238 Query: 243 GKNEKLLREMQGNPLYSHPNISLVGFTREIDKLMEISDLLVTKPGGMTCSEGLAKGIPML 302 G+N+KL+ M+ +P++ HPNI ++G+ +I KLM++SDLL+TKPGGMTC+EG AKGIPML Sbjct: 239 GQNDKLVASMREDPIFQHPNIHVLGYRDDIHKLMDVSDLLITKPGGMTCTEGAAKGIPML 298 Query: 303 FYDPLPGQEEENCRYFTAAGLGEPVTSLKVVDRWMERLLYSYDEVQAKRQS 353 FY+P+PGQEE N +F + G GE + S V+D+W+ L YD+V+ KR Sbjct: 299 FYEPIPGQEEVNSHFFVSEGYGEILDSPAVIDKWLNLLSDHYDQVEKKRNG 349 >B8I3U5.1 B8I3U5_CLOCE Monogalactosyldiacylglycerol synthase Length = 370 Score = 210 bits (534), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 108/325 (33%), Positives = 182/325 (56%), Gaps = 3/325 (0%) Query: 9 RVLLLSEGFGAGHTQAAYALSSSLRKLSPNLQTKVLELGSFLNPRLAPLIVSAYRKTVTS 68 RVL+L G GH +AA AL S+ + P+ + VL+ ++NP + ++VS+Y T+ Sbjct: 2 RVLILYVSVGTGHMKAAEALKESIERQFPDWKVDVLDALKYINPVIDKIVVSSYLGTLKR 61 Query: 69 QPRLMGYVYRHQKSFNRL--TTLALHRIFYTHTQNIMKQLKPDIIVCTHFIPSAVVSRLK 126 P+L +Y + + T+ A++++ ++++ KP +VCTH P ++S LK Sbjct: 62 NPKLYSMIYTASGTGTGIYDTSKAVNKLLSYKLKSLINDYKPSAVVCTHPFPMQMLSSLK 121 Query: 127 RLDPAFKVPLVTVITDYDAHATWISPEVDRYLVSTPEVKTKLRYRSVAAAKIQVTGMPVH 186 R + +P + ++TDY H+ W+ +D ++V+ +K+++ R + I G+PV Sbjct: 122 RKN-KLNIPTMAILTDYVVHSLWLDSGMDAFIVANDNMKSEMIRRGIPDNIIFPYGIPVS 180 Query: 187 PSFWEHPGKADILEQFGLQDMPTVLVMGGGWGMMNDEVINTALAGWRDKLQIIFCLGKNE 246 P F K D+L ++G++D TVLVMGGG G N E +L +QII G N+ Sbjct: 181 PKFLASTNKKDLLLKYGMEDKFTVLVMGGGMGFGNIEKTMASLLDCNVDIQIIAVTGTNQ 240 Query: 247 KLLREMQGNPLYSHPNISLVGFTREIDKLMEISDLLVTKPGGMTCSEGLAKGIPMLFYDP 306 KL +++ S + ++ +T +++LM+ISDLL+TKPGGMT SE L KG+P+ P Sbjct: 241 KLKFQLEEYAAQSSKKVLILSYTDRVNELMDISDLLITKPGGMTVSEALVKGLPIFIISP 300 Query: 307 LPGQEEENCRYFTAAGLGEPVTSLK 331 +PGQEE N + +G+ + S Sbjct: 301 IPGQEEGNANFLIRSGVANKIDSFN 325 >A7FPG9.1 A7FPG9_CLOB1 Putative uncharacterized protein Length = 413 Score = 204 bits (519), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 111/339 (32%), Positives = 184/339 (54%), Gaps = 5/339 (1%) Query: 9 RVLLLSEGFGAGHTQAAYALSSSLRKLSPNLQTKVLELGSFLNPRLAPLIVSAYRKTVTS 68 ++L+LS G GH AA AL S ++ + + V++ ++NP + +I+ +Y KT+ Sbjct: 2 KILILSVSAGGGHIHAAEALKSYIKLNNNKAEITVIDTLKYINPLIDKVIIGSYLKTLKV 61 Query: 69 QPRLMGYVYRHQKSFNRLTTL---ALHRIFYTHTQNIMKQLKPDIIVCTHFIPSAVVSRL 125 P L G +Y H + L T+ L +I +++ +L PDII+CTH P+ ++S + Sbjct: 62 TPSLFGKLYDHSEDDEGLATVISSNLSKIMTYKLSHLVNELNPDIIICTHPFPAEMISIM 121 Query: 126 KRLDPAFKVPLVTVITDYDAHATWISPEVDRYLVSTPEVKTKLRYRSVAAAKIQVTGMPV 185 K +P +T++TDY H+ WI D Y+VS ++ ++ R+V KI G+PV Sbjct: 122 KD-KGKLNIPSLTILTDYAPHSFWIQEHTDAYVVSNSDMIDEMVARNVPKNKIFDFGIPV 180 Query: 186 HPSFWEHPGKADILEQFGLQ-DMPTVLVMGGGWGMMNDEVINTALAGWRDKLQIIFCLGK 244 +PSF + K L++ L ++PT L+MGG G+ + + L +QII G Sbjct: 181 NPSFLKKYDKEKTLKELDLNINIPTFLIMGGSLGIGKISDLYSELIKIDQNMQIIIITGN 240 Query: 245 NEKLLREMQGNPLYSHPNISLVGFTREIDKLMEISDLLVTKPGGMTCSEGLAKGIPMLFY 304 N+KL E+ S+ ++GFT +++K M+ DLL+TKPGG+T +E L IPM + Sbjct: 241 NKKLYSELNKLKENSNKETRIIGFTDKVNKYMQCCDLLLTKPGGLTITEALVSNIPMAVF 300 Query: 305 DPLPGQEEENCRYFTAAGLGEPVTSLKVVDRWMERLLYS 343 P+PGQEE+N ++ L + S++ + LL S Sbjct: 301 SPIPGQEEKNAKFLLRHNLAISIDSIEDTKDIISDLLKS 339 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,252,034,260 Number of extensions: 93745745 Number of successful extensions: 226413 Number of sequences better than 1.0e-05: 358 Number of HSP's gapped: 225701 Number of HSP's successfully gapped: 359 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)