BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0049.p (249 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB11.1 B3KB11_9BACI tRNA (Guanine-N(7)-)-methyltransferase EC=... 375 e-102 B1DL67.1 B1DL67_9BACL tRNA (Guanine-N(7)-)-methyltransferase EC=... 318 3e-85 Q030M2.1 TRMB_LACLS tRNA (guanine-N(7)-)-methyltransferase EC=2.... 213 1e-53 A2RM55.1 TRMB_LACLM tRNA (guanine-N(7)-)-methyltransferase EC=2.... 213 2e-53 Q9CHI2.1 TRMB_LACLA tRNA (guanine-N(7)-)-methyltransferase EC=2.... 213 2e-53 >B3KB11.1 B3KB11_9BACI tRNA (Guanine-N(7)-)-methyltransferase EC=2.1.1.33 Length = 237 Score = 375 bits (962), Expect = e-102, Method: Compositional matrix adjust. Identities = 177/236 (75%), Positives = 201/236 (85%), Gaps = 1/236 (0%) Query: 12 MRLRGRKGIRESLEEQTDLVILDPRSLKGQWSELFGNDHPIHVEFGMGKGQFISRMSFKY 71 MRLRGRKGIRE+LE+Q DLV+LDP +G+W+ LFGND PIHVEFGMGKGQFIS+MS+K+ Sbjct: 1 MRLRGRKGIRENLEQQEDLVVLDPGQYRGKWASLFGNDLPIHVEFGMGKGQFISQMSYKH 60 Query: 72 PDINFIGVDMYDELIRRAAEKARAVWEPAGQETPPNLRVALANINYAEEVFAPGELERIY 131 +NFIGVDMYDELIRRA+EK R VW G +TPPNL++AL NI EEVF PGE+ER+Y Sbjct: 61 QQVNFIGVDMYDELIRRASEKGRNVWAQEGVDTPPNLKLALFNIENVEEVFEPGEIERLY 120 Query: 132 LNFSDPWPKSKHARRRLTHPRFLDKYRGLLSPLGEIHLKTDSRSLFEFSLNAFADYGLQM 191 LNFSDPWPK+KHARRRLTHPRFLDKY +L+ GEIH KTDS +LFEFSLNAFADYGLQM Sbjct: 121 LNFSDPWPKTKHARRRLTHPRFLDKYSRILNERGEIHFKTDSVTLFEFSLNAFADYGLQM 180 Query: 192 KNISLDLHPDGVMNEEHVMTEYETKFFGRGVNIHRCEAIVGAEALARYQATRLDKY 247 NISLDLH DG +NEEHVMTEYETKF G+G+ IHRCEAIVG EALA Y RL+KY Sbjct: 181 TNISLDLHRDG-LNEEHVMTEYETKFVGQGMQIHRCEAIVGKEALASYHQNRLEKY 235 >B1DL67.1 B1DL67_9BACL tRNA (Guanine-N(7)-)-methyltransferase EC=2.1.1.33 Length = 254 Score = 318 bits (815), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 154/237 (64%), Positives = 185/237 (78%), Gaps = 1/237 (0%) Query: 12 MRLRGRKGIRESLEEQTDLVILDPRSLKGQWSELFGNDHPIHVEFGMGKGQFISRMSFKY 71 MRLRGRKGIRESLE Q +LV+LD + LKG+W E FGND PIHVE GMGKG+FIS MSF+ Sbjct: 1 MRLRGRKGIRESLEAQPELVVLDAKPLKGKWREFFGNDKPIHVELGMGKGRFISNMSFRN 60 Query: 72 PDINFIGVDMYDELIRRAAEKARAVWEPAGQETPPNLRVALANINYAEEVFAPGELERIY 131 PD N+IGVDMYDELIRRA++KAR +WE +G E PPNL + ANI + EE+F PGE++RIY Sbjct: 61 PDNNYIGVDMYDELIRRASDKARNIWEESGVEEPPNLGLLRANIEHIEEMFEPGEIQRIY 120 Query: 132 LNFSDPWPKSKHARRRLTHPRFLDKYRGLLSPLGEIHLKTDSRSLFEFSLNAFADYGLQM 191 LNFSDPWPKSKHARRRLTHPRFL KY LL GEIH KTDS SLFEFSLN+FA+ Q+ Sbjct: 121 LNFSDPWPKSKHARRRLTHPRFLRKYAQLLDERGEIHFKTDSESLFEFSLNSFAEVEFQL 180 Query: 192 KNISLDLHPDGVMNEEHVMTEYETKFFGRGVNIHRCEAIVGAEALARYQATRLDKYR 248 +NISL+LH +G E+ V TEYE KF +G I+R EA++G L Y+ ++L+ R Sbjct: 181 RNISLNLHKNGP-REDLVFTEYEMKFMEKGQPIYRLEAVIGKTVLREYRESKLENSR 236 >Q030M2.1 TRMB_LACLS tRNA (guanine-N(7)-)-methyltransferase EC=2.1.1.33 Length = 217 Score = 213 bits (541), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 104/218 (47%), Positives = 143/218 (65%), Gaps = 7/218 (3%) Query: 12 MRLRGRKGIRESLEEQTDLVILDPRSLKGQWSELFGNDHPIHVEFGMGKGQFISRMSFKY 71 MR+R RKG E L E +V+ +P KG WSE FGNDHPIH+E G GKG FI+ M+ + Sbjct: 1 MRVRNRKGAGEMLAENAHIVVENPADFKGSWSERFGNDHPIHIEVGCGKGAFITGMAALH 60 Query: 72 PDINFIGVDMYDELIRRAAEKARAVWEPAGQETPPNLRVALANINYAEEVFAPGELERIY 131 P+IN+I +DM ++ A +KA + PN+++ L + E FA GE++++Y Sbjct: 61 PEINYIAIDMQLSVLSYALDKAI-------EADLPNVQMMLVDGAALSEYFADGEIDQVY 113 Query: 132 LNFSDPWPKSKHARRRLTHPRFLDKYRGLLSPLGEIHLKTDSRSLFEFSLNAFADYGLQM 191 LNFSDPWPK +H +RRLT+ FL Y +L P GEIH KTD+R LFE+SL + A+YG+++ Sbjct: 114 LNFSDPWPKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFEYSLVSLANYGMEL 173 Query: 192 KNISLDLHPDGVMNEEHVMTEYETKFFGRGVNIHRCEA 229 K + LDLH D ++VMTEYE KF +G I+R EA Sbjct: 174 KKVWLDLHQDEEFAPQNVMTEYEQKFSQKGQVIYRLEA 211 >A2RM55.1 TRMB_LACLM tRNA (guanine-N(7)-)-methyltransferase EC=2.1.1.33 Length = 217 Score = 213 bits (541), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 104/218 (47%), Positives = 144/218 (66%), Gaps = 7/218 (3%) Query: 12 MRLRGRKGIRESLEEQTDLVILDPRSLKGQWSELFGNDHPIHVEFGMGKGQFISRMSFKY 71 MR+R RKG E L E +V+ +P KG+WSE FGNDHPIH+E G GKG FI+ M+ + Sbjct: 1 MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALH 60 Query: 72 PDINFIGVDMYDELIRRAAEKARAVWEPAGQETPPNLRVALANINYAEEVFAPGELERIY 131 P+IN+I +DM ++ A +KA + PN+++ L + E FA GE++++Y Sbjct: 61 PEINYIAIDMQLSVLSYALDKAI-------EADLPNVQMMLVDGAALSEYFADGEIDQVY 113 Query: 132 LNFSDPWPKSKHARRRLTHPRFLDKYRGLLSPLGEIHLKTDSRSLFEFSLNAFADYGLQM 191 LNFSDPWPK +H +RRLT+ FL Y +L P GEIH KTD+R LFE+SL + A+YG+++ Sbjct: 114 LNFSDPWPKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFEYSLVSLANYGMEL 173 Query: 192 KNISLDLHPDGVMNEEHVMTEYETKFFGRGVNIHRCEA 229 K + LDLH D ++VMTEYE KF +G I+R EA Sbjct: 174 KKVWLDLHQDEEFAPQNVMTEYEQKFSQKGQVIYRLEA 211 >Q9CHI2.1 TRMB_LACLA tRNA (guanine-N(7)-)-methyltransferase EC=2.1.1.33 Length = 217 Score = 213 bits (541), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 104/218 (47%), Positives = 144/218 (66%), Gaps = 7/218 (3%) Query: 12 MRLRGRKGIRESLEEQTDLVILDPRSLKGQWSELFGNDHPIHVEFGMGKGQFISRMSFKY 71 MR+R RKG E L E +V+ +P KG+WSE FGNDHPIH+E G GKG FI+ M+ + Sbjct: 1 MRVRNRKGAGEMLAENAHIVVENPADFKGRWSERFGNDHPIHIEVGCGKGAFITGMAALH 60 Query: 72 PDINFIGVDMYDELIRRAAEKARAVWEPAGQETPPNLRVALANINYAEEVFAPGELERIY 131 P+IN+I +DM ++ A +KA + PN+++ L + E FA GE++++Y Sbjct: 61 PEINYIAIDMQLSVLSYALDKAI-------EADLPNVQMMLVDGAALSEYFADGEIDQVY 113 Query: 132 LNFSDPWPKSKHARRRLTHPRFLDKYRGLLSPLGEIHLKTDSRSLFEFSLNAFADYGLQM 191 LNFSDPWPK +H +RRLT+ FL Y +L P GEIH KTD+R LFE+SL + A+YG+++ Sbjct: 114 LNFSDPWPKGRHEKRRLTYKSFLATYEKILRPEGEIHFKTDNRGLFEYSLVSLANYGMEL 173 Query: 192 KNISLDLHPDGVMNEEHVMTEYETKFFGRGVNIHRCEA 229 K + LDLH D ++VMTEYE KF +G I+R EA Sbjct: 174 KKVWLDLHQDEEFAPQNVMTEYEQKFSQKGQVIYRLEA 211 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,538,206,284 Number of extensions: 66744241 Number of successful extensions: 146153 Number of sequences better than 1.0e-05: 1223 Number of HSP's gapped: 144734 Number of HSP's successfully gapped: 1223 Length of query: 249 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 117 Effective length of database: 1,817,122,223 Effective search space: 212603300091 Effective search space used: 212603300091 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)