BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0044.p (253 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB07.1 B3KB07_9BACI Mannose-6-phosphate isomerase, class I EC=... 428 e-118 Q67MX5.1 Q67MX5_SYMTH Mannnose-6 phospate isomerase 249 1e-64 B8D0G2.1 B8D0G2_HALOH Mannose-6-phosphate isomerase, class I EC=... 244 4e-63 C4VFX6.1 C4VFX6_ENTFA Mannose-6-phosphate isomerase, class I EC=... 226 2e-57 C2H2W0.1 C2H2W0_ENTFA Mannose-6-phosphate isomerase, class I 226 2e-57 >B3KB07.1 B3KB07_9BACI Mannose-6-phosphate isomerase, class I EC=5.3.1.8 Length = 322 Score = 428 bits (1100), Expect = e-118, Method: Compositional matrix adjust. Identities = 199/238 (83%), Positives = 223/238 (93%) Query: 1 MTKPYPLKFQPEFKERVWGGRALEKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQG 60 MTKPYPL FQPEFKERVWGGRALE+FGL++PEGHIGEGWMIADHPNGT+S++NGELAG+G Sbjct: 1 MTKPYPLLFQPEFKERVWGGRALEQFGLDIPEGHIGEGWMIADHPNGTTSIVNGELAGRG 60 Query: 61 LDQIRLQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPKGELGKTE 120 LD++R QFG EWFGSKG + GRFPLLIKLLDCNDNLSVQVHPTDDYE LPKGELGKTE Sbjct: 61 LDEVREQFGREWFGSKGFSEVNGRFPLLIKLLDCNDNLSVQVHPTDDYEGLPKGELGKTE 120 Query: 121 MWYVLDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHAL 180 MWYVLDAKP AKIIYGLK+GV RE+L+EA+E+GTVMD ++E++V AGD FYIPAGTVHAL Sbjct: 121 MWYVLDAKPDAKIIYGLKEGVNRETLKEAMENGTVMDQLQEVSVKAGDTFYIPAGTVHAL 180 Query: 181 CAGVVVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNVTAYEGAGATSMKTDGA 238 CAGVVVAEIQQNSDTTYRI+DY+RPGLDGKPRELHIEDSLNVTAYEG+GAT+M T+G Sbjct: 181 CAGVVVAEIQQNSDTTYRIFDYNRPGLDGKPRELHIEDSLNVTAYEGSGATTMSTEGT 238 >Q67MX5.1 Q67MX5_SYMTH Mannnose-6 phospate isomerase Length = 324 Score = 249 bits (637), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 123/224 (54%), Positives = 158/224 (70%), Gaps = 4/224 (1%) Query: 5 YPLKFQPEFKERVWGGRALEKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQI 64 YPL+F P FKERVWGGR L +F +LP G IGE W+++DHP G + + NG AG+ L + Sbjct: 3 YPLRFTPLFKERVWGGRRLARFYPDLPPGPIGEAWVLSDHPQGRTPIANGPWAGETLSGL 62 Query: 65 RLQFGHE----WFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPKGELGKTE 120 R E G++ + FPLL KLLD D+LS+QVHP DD+ LP GE GKTE Sbjct: 63 REHLPPERDAALMGARVLARGEEGFPLLFKLLDAQDDLSIQVHPADDFPGLPPGERGKTE 122 Query: 121 MWYVLDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHAL 180 MW VL+A+PGA+I++GL +GVTR+ L A+ G ++ MR +TV GD +Y+PAG VHAL Sbjct: 123 MWVVLEAEPGARIVHGLAEGVTRDGLAAAVREGRTVEAMRFLTVRPGDVYYLPAGLVHAL 182 Query: 181 CAGVVVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNVTA 224 AG+VVAEIQQ+SDTTYR+YDY R GLDGKPRELHI+ +L + Sbjct: 183 GAGLVVAEIQQSSDTTYRLYDYGRLGLDGKPRELHIDQALACCS 226 >B8D0G2.1 B8D0G2_HALOH Mannose-6-phosphate isomerase, class I EC=5.3.1.8 Length = 353 Score = 244 bits (624), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 115/223 (51%), Positives = 156/223 (69%), Gaps = 1/223 (0%) Query: 5 YPLKFQPEFKERVWGGRALEK-FGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQ 63 YPLKF+P +K ++WGGR L+ F +LP+ +IGE W +A HPNGTS V NG L GQ L Sbjct: 4 YPLKFKPVYKSKIWGGRKLKDVFNRDLPDQNIGESWEVAAHPNGTSIVANGSLKGQSLPD 63 Query: 64 IRLQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPKGELGKTEMWY 123 I +G + G K RFPLLIKLLD LSVQVHP +DY + + GKTEMWY Sbjct: 64 IINTYGDKITGEKLVDICQERFPLLIKLLDAEKKLSVQVHPDNDYAHRVENDSGKTEMWY 123 Query: 124 VLDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCAG 183 ++DA+PGA+++YGLK G T+E L +A++ G + + + V GD F++P+GT+HA+ G Sbjct: 124 IIDARPGARLVYGLKPGTTKEQLAKAIKRGEIEKYLNRVPVKKGDVFFMPSGTIHAIEEG 183 Query: 184 VVVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNVTAYE 226 +++AEIQQNSDTTYR+YD+DR G DG PR LHIE +L+V ++ Sbjct: 184 ILLAEIQQNSDTTYRVYDWDRVGQDGNPRPLHIEKALDVINFK 226 >C4VFX6.1 C4VFX6_ENTFA Mannose-6-phosphate isomerase, class I EC=5.3.1.8 Length = 323 Score = 226 bits (575), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 113/220 (51%), Positives = 150/220 (68%), Gaps = 7/220 (3%) Query: 6 PLKFQPEFKERVWGGRALEK-FGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQI 64 PL QP F+E++WGG L FG +LP IGE W I+ HP+G S+VLNGE G+ LD++ Sbjct: 4 PLFLQPVFQEKIWGGNRLHTLFGFDLPSDKIGEDWAISAHPHGVSTVLNGEFKGKKLDEL 63 Query: 65 RLQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPKGELGKTEMWYV 124 E FG G G FPLL K+LD D+LSVQVHP D Y + +GELGKTE WY+ Sbjct: 64 WADH-QELFGHAG----GAVFPLLTKILDAEDDLSVQVHPDDAYGQKHEGELGKTECWYI 118 Query: 125 LDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCAGV 184 +DA+PGA+IIYG T+E L E +E G D +R++ V GD +Y+P+GT+HA+ +G+ Sbjct: 119 IDAEPGAEIIYG-HHAKTKEELAEMIEEGRWDDLLRKVPVKKGDFYYVPSGTIHAIGSGI 177 Query: 185 VVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNVTA 224 ++ E QQ+SDTTYR+YDYDR GK RELHI+ S++VT Sbjct: 178 LILETQQSSDTTYRVYDYDRTDDQGKTRELHIQQSIDVTT 217 >C2H2W0.1 C2H2W0_ENTFA Mannose-6-phosphate isomerase, class I Length = 323 Score = 226 bits (575), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 113/220 (51%), Positives = 150/220 (68%), Gaps = 7/220 (3%) Query: 6 PLKFQPEFKERVWGGRALEK-FGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQI 64 PL QP F+E++WGG L FG +LP IGE W I+ HP+G S+VLNGE G+ LD++ Sbjct: 4 PLFLQPVFQEKIWGGNRLHTLFGFDLPSDKIGEDWAISAHPHGVSTVLNGEFKGKKLDEL 63 Query: 65 RLQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPKGELGKTEMWYV 124 E FG G G FPLL K+LD D+LSVQVHP D Y + +GELGKTE WY+ Sbjct: 64 WADH-QELFGHAG----GAVFPLLTKILDAEDDLSVQVHPDDAYGQKHEGELGKTECWYI 118 Query: 125 LDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCAGV 184 +DA+PGA+IIYG T+E L E +E G D +R++ V GD +Y+P+GT+HA+ +G+ Sbjct: 119 IDAEPGAEIIYG-HHAKTKEELAEMIEEGRWDDLLRKVPVKKGDFYYVPSGTIHAIGSGI 177 Query: 185 VVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNVTA 224 ++ E QQ+SDTTYR+YDYDR GK RELHI+ S++VT Sbjct: 178 LILETQQSSDTTYRVYDYDRTDDQGKTRELHIQQSIDVTT 217 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,717,488,771 Number of extensions: 77770629 Number of successful extensions: 174033 Number of sequences better than 1.0e-05: 522 Number of HSP's gapped: 172679 Number of HSP's successfully gapped: 522 Length of query: 253 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 120 Effective length of database: 1,807,701,208 Effective search space: 216924144960 Effective search space used: 216924144960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 129 (54.3 bits)