BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0036.p (551 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5F0.1 B3K5F0_9BACI GerA spore germination protein 566 e-159 B1DG93.1 B1DG93_9BACL GerA spore germination protein 564 e-159 C0Z9D8.1 C0Z9D8_BREBN Spore germination protein 417 e-114 C2N2W1.1 C2N2W1_BACCE Uncharacterized membrane protein yndD 416 e-114 B5UM88.1 B5UM88_BACCE Spore germination protein GerAA 416 e-114 >B3K5F0.1 B3K5F0_9BACI GerA spore germination protein Length = 542 Score = 566 bits (1459), Expect = e-159, Method: Compositional matrix adjust. Identities = 274/489 (56%), Positives = 364/489 (74%), Gaps = 4/489 (0%) Query: 29 KLSLSLSANLERLLNRLGQSSDLVVRDFLLFGRYRAALVFFSSLVNQEQIQEHILKPLMA 88 +S SL + LG + D ++ F +FG++ A L +FS + + I +L+PL Sbjct: 29 NISESLEETTSEIKKILGDNDDFMIHPFHVFGQHPAVLFYFSDMTDHAVINNTLLRPLKY 88 Query: 89 --RSDDHPGLPDNPAGLSHYIWNTALQVTQGTTEEDLSALPSGIVKGQLILLIEGSAEAL 146 R D LP + L + L ++G +E +S L +++GQ ++ IEG EA+ Sbjct: 89 GPRHLDVSDLPRH--ALMDTLMKDTLYHSEGYSERKISVLTDNLLRGQSVVAIEGLDEAI 146 Query: 147 VLDMRQVDTRGVEQPQTEQVIRGPREGYVEKLENNLSLLRYRLQSTDFRIEISPIGSRTQ 206 ++D R++ R +EQP TEQVIRG REG++E L N+SLLRYRLQ+ DFR++ IG++T+ Sbjct: 147 IIDTRKISQRSIEQPATEQVIRGAREGFIETLGTNISLLRYRLQTPDFRVKSMEIGTKTK 206 Query: 207 SRVALCYLDSLADPKLVAEVMRRISMIQTDGIIDAGYIEQFIEDQPLSPFPQVQSTERPD 266 S+VA+CY+D + +P +V EV +R+ I+ D ++D+GY+EQFIED SPFPQ+Q TERPD Sbjct: 207 SKVAVCYMDGITNPGIVKEVNKRLDAIEIDAVLDSGYLEQFIEDNHWSPFPQIQYTERPD 266 Query: 267 KTVAGLLEGRVAILVDGSPFALIVPALFNQFFQTVDDYTERFIMGXXXXXXXXXXXXXXX 326 K VA LLEGRVAILVDGSP ALI P +F+QF+QTV+DYTERF++ Sbjct: 267 KVVANLLEGRVAILVDGSPLALIAPTVFSQFYQTVEDYTERFLLMSAIRLSRLVALVFSL 326 Query: 327 XFPALYVSVISFNPELMPTDFAVAISGGRAGVPFPAVLEVLIMEVSMEVLREATIRLPQM 386 FP+LYV++ISFNPEL+PT+FAVA++GGRAGVPFPA++EVL++E+SMEVLREATIRLPQ Sbjct: 327 VFPSLYVAIISFNPELIPTEFAVAVAGGRAGVPFPAMVEVLVIEISMEVLREATIRLPQQ 386 Query: 387 IGGALSIVGVLVIGQAAVAAGLASPITVVIVALTTIGSFATPAYNAAIALRMLRFPLILL 446 +GGALSIVGVLVIGQAAV+AG ASPIT+VI+ALTTIGSFATPAYNAA+ALR+LRFPLI+L Sbjct: 387 VGGALSIVGVLVIGQAAVSAGFASPITIVIIALTTIGSFATPAYNAALALRLLRFPLIIL 446 Query: 447 AGMFGLYGVMIGTILIINHLLFLESFAVPYMSPYIPGKWRDWKDSLVRVPLWWMRSRPSF 506 AG+FGLYGVM+G ILI NHLL L+SF VPY+SP++PG ++ +D L R PLW M++RPSF Sbjct: 447 AGIFGLYGVMVGIILIANHLLSLKSFGVPYLSPFVPGNFQGMRDLLTRGPLWKMKNRPSF 506 Query: 507 LHTRDNTRL 515 LH D +RL Sbjct: 507 LHPLDTSRL 515 >B1DG93.1 B1DG93_9BACL GerA spore germination protein Length = 539 Score = 564 bits (1454), Expect = e-159, Method: Compositional matrix adjust. Identities = 290/503 (57%), Positives = 368/503 (73%), Gaps = 7/503 (1%) Query: 23 PEPGK-------EKLSLSLSANLERLLNRLGQSSDLVVRDFLLFGRYRAALVFFSSLVNQ 75 P P K +++S SL + L LG + D VR F +FG++ AA++FFS +VNQ Sbjct: 16 PSPSKTMDKYKGQQVSGSLGDTIAFLDGILGINEDFTVRKFNVFGQFPAAIMFFSCMVNQ 75 Query: 76 EQIQEHILKPLMARSDDHPGLPDNPAGLSHYIWNTALQVTQGTTEEDLSALPSGIVKGQL 135 + + I+K LM G LS I N +L ++ + + +L+AL I++G Sbjct: 76 DSVNSDIMKALMYTPPHLIGRTIETNQLSDVIMNDSLYHSKSSLQGELTALVDCILRGDT 135 Query: 136 ILLIEGSAEALVLDMRQVDTRGVEQPQTEQVIRGPREGYVEKLENNLSLLRYRLQSTDFR 195 ++ ++G E++++ R V+ R +EQP TEQVIRGPREG++E L N+SL+RYRLQS D R Sbjct: 136 VIAVDGMKESIIITTRSVEKRSIEQPATEQVIRGPREGFIEPLGTNISLIRYRLQSPDLR 195 Query: 196 IEISPIGSRTQSRVALCYLDSLADPKLVAEVMRRISMIQTDGIIDAGYIEQFIEDQPLSP 255 I+ IG RT+S+VA+CY+D + +P LV EV +R+S I D ++D+GY+EQFIED SP Sbjct: 196 IQTMEIGRRTKSKVAVCYMDGITNPALVEEVHKRLSKIDIDTVLDSGYLEQFIEDNHYSP 255 Query: 256 FPQVQSTERPDKTVAGLLEGRVAILVDGSPFALIVPALFNQFFQTVDDYTERFIMGXXXX 315 FPQVQ TERPDK VA L EGRV+ILVDGSP AL+VP +FNQF+QTV+DYTERF++ Sbjct: 256 FPQVQYTERPDKVVANLAEGRVSILVDGSPLALVVPTVFNQFYQTVEDYTERFLLMSSIR 315 Query: 316 XXXXXXXXXXXXFPALYVSVISFNPELMPTDFAVAISGGRAGVPFPAVLEVLIMEVSMEV 375 FPALYVS+IS+NPEL+PTDFAVA++GGRAGVPFPAV+EV+I+E+SMEV Sbjct: 316 LARLIALVFSLVFPALYVSIISYNPELIPTDFAVAVAGGRAGVPFPAVIEVIIIEISMEV 375 Query: 376 LREATIRLPQMIGGALSIVGVLVIGQAAVAAGLASPITVVIVALTTIGSFATPAYNAAIA 435 LREATIRLPQ +GGALSIVGVLV+GQAAVAAG ASPITVVI+ALTTIGSFATPAYNAA+A Sbjct: 376 LREATIRLPQQVGGALSIVGVLVVGQAAVAAGFASPITVVIIALTTIGSFATPAYNAALA 435 Query: 436 LRMLRFPLILLAGMFGLYGVMIGTILIINHLLFLESFAVPYMSPYIPGKWRDWKDSLVRV 495 LR+LRFPLI+LAG FGLYGVMIG ILI NHLL L+SF VPY+SP IP ++ KD LVR Sbjct: 436 LRLLRFPLIILAGTFGLYGVMIGLILIANHLLSLKSFGVPYLSPLIPVDFQGLKDLLVRG 495 Query: 496 PLWWMRSRPSFLHTRDNTRLPAS 518 PLW M RP+FL D TR+ S Sbjct: 496 PLWLMNKRPAFLDPVDETRVSES 518 >C0Z9D8.1 C0Z9D8_BREBN Spore germination protein Length = 513 Score = 417 bits (1073), Expect = e-114, Method: Compositional matrix adjust. Identities = 213/518 (41%), Positives = 332/518 (64%), Gaps = 19/518 (3%) Query: 1 MQAWKEKY--VSSIQKQKQPSPAPPEPGKEKLSLSLSANLERLLNRLGQSSDLVVRDFLL 58 +QAWK++ + S ++ + L+ ++ N++ + + G+ DLVVR F Sbjct: 5 LQAWKKRKEALQSKTLEQHSDKSMESVSDVALTTNVEENIQLIQAQFGKCDDLVVRRFQT 64 Query: 59 FGRYRAALVFFSSLVNQEQIQEHILKPLMARSDDHPGLPDNPAGLSHYIWNTALQVTQGT 118 + +AA+VF +V++ I + I++ + + ++PA L +T Sbjct: 65 KTKTQAAIVFIDGMVDRNVISDFIIRYMTTPDITY----NDPA-------TNELDLT--- 110 Query: 119 TEEDLSALPSGIVKGQLILLIEGSAEALVLDMRQVDTRGVEQPQTEQVIRGPREGYVEKL 178 + L + I+ G +L+++G A + + R D R VE+PQTE V+RGPR+G+ E L Sbjct: 111 --DKLRQIVRNILSGCAVLILDGCQNAYLNNTRGWDRRSVEEPQTESVVRGPRDGFCETL 168 Query: 179 ENNLSLLRYRLQSTDFRIEISPIGSRTQSRVALCYLDSLADPKLVAEVMRRI-SMIQTDG 237 N +L+R+RL+ + R+ +G RTQ+ + + Y++ +A P +V EV+ RI S ++ D Sbjct: 169 CVNSALIRFRLKDPNLRVRHMVVGERTQTDIYIMYVEGIAYPPMVEEVIARIESNVRVDS 228 Query: 238 IIDAGYIEQFIEDQPLSPFPQVQSTERPDKTVAGLLEGRVAILVDGSPFALIVPALFNQF 297 ++++GYIEQ I+D+ SPFPQ+Q+TERPDK VA LLEG+V ILVDG+PF LI PA+F+QF Sbjct: 229 VLESGYIEQMIQDRRWSPFPQIQNTERPDKVVANLLEGKVGILVDGTPFGLIAPAVFSQF 288 Query: 298 FQTVDDYTERFIMGXXXXXXXXXXXXXXXXFPALYVSVISFNPELMPTDFAVAISGGRAG 357 +Q+ +DY ERF + P+LY++ SF+PE++P+ +A++ GR+ Sbjct: 289 YQSPEDYYERFYIATLIRFIRIISISIALLLPSLYIAFSSFHPEMIPSRLVIAMAAGRST 348 Query: 358 VPFPAVLEVLIMEVSMEVLREATIRLPQMIGGALSIVGVLVIGQAAVAAGLASPITVVIV 417 VPFP+++E L ME+++E+LREA++RLP IG + IVG LV+G+AAV AGL SP+ V+IV Sbjct: 349 VPFPSLIEALSMELAIEILREASVRLPGPIGPTIGIVGALVVGEAAVTAGLVSPVMVIIV 408 Query: 418 ALTTIGSFATPAYNAAIALRMLRFPLILLAGMFGLYGVMIGTILIINHLLFLESFAVPYM 477 A+TTIGSFA+P+Y+AAIA+RMLRFP+++LAGMFGLYG+M+ I+I+ HL L+SF VPYM Sbjct: 409 AVTTIGSFASPSYSAAIAIRMLRFPVMILAGMFGLYGIMLFLIVILIHLSSLKSFGVPYM 468 Query: 478 SPYIPGKWRDWKDSLVRVPLWWMRSRPSFLHTRDNTRL 515 SP P KD +R P +++RP H +D R+ Sbjct: 469 SPLSPLNLNGMKDLFIRAPHHMLKTRPMMFHVQDKIRI 506 >C2N2W1.1 C2N2W1_BACCE Uncharacterized membrane protein yndD Length = 527 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 219/523 (41%), Positives = 322/523 (61%), Gaps = 10/523 (1%) Query: 1 MQAWK--EKYV--SSIQKQKQPSPAPPEPGKEKLSLSLSANLERLLNRLGQSSDLVVRDF 56 M WK +K V ++IQ + + P + + ++NLE + ++G +SD+ R F Sbjct: 4 MSWWKSRDKQVKKNNIQSKTKQEHKPSQNANVHFTNDFASNLELIKQQIGHNSDVRFRKF 63 Query: 57 LLFGR--YRAALVFFSSLVNQEQIQEHILKPLMAR-SDDHPGLPDNPAGL--SHYIWNTA 111 + GR +A +VF L ++E I +HI+K LM SD++ G YI N Sbjct: 64 NI-GRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSDEYKNESSYVEGTVSKEYIKNKV 122 Query: 112 LQVTQGTTEEDLSALPSGIVKGQLILLIEGSAEALVLDMRQVDTRGVEQPQTEQVIRGPR 171 L +++ + L ++ G LLI+G A+ +L + + R +E+P +E ++RGPR Sbjct: 123 LTISEVAEVHYIKDLIPKVLIGSTALLIDGLADVFILGTTKANKRNIEEPVSEALVRGPR 182 Query: 172 EGYVEKLENNLSLLRYRLQSTDFRIEISPIGSRTQSRVALCYLDSLADPKLVAEVMRRIS 231 G+ E L +N SLLR + + P+G R + + + ++ LA +LV E+ RI Sbjct: 183 VGFTEVLSDNTSLLRRHCADENLSLVKLPVGERAKKELVVAFIKDLASTELVDEIKNRIQ 242 Query: 232 MIQTDGIIDAGYIEQFIEDQPLSPFPQVQSTERPDKTVAGLLEGRVAILVDGSPFALIVP 291 I D ++++GYIEQ IED LSPFPQ+Q+TERPD+ ++ L+EGRVAIL+DG+PF LI P Sbjct: 243 KIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVISALMEGRVAILLDGTPFVLIAP 302 Query: 292 ALFNQFFQTVDDYTERFIMGXXXXXXXXXXXXXXXXFPALYVSVISFNPELMPTDFAVAI 351 F+ Q+ +DY ER++ G PALY+S ISF+P L+PT A++I Sbjct: 303 VTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAPALYISFISFHPGLIPTKLAISI 362 Query: 352 SGGRAGVPFPAVLEVLIMEVSMEVLREATIRLPQMIGGALSIVGVLVIGQAAVAAGLASP 411 G R GVPFPA++E LIMEV++EVLREA +RLP+ IG A+ IVG L+IGQAAV AG+ SP Sbjct: 363 IGSREGVPFPALIEALIMEVAIEVLREAGLRLPKPIGPAMGIVGGLIIGQAAVEAGIVSP 422 Query: 412 ITVVIVALTTIGSFATPAYNAAIALRMLRFPLILLAGMFGLYGVMIGTILIINHLLFLES 471 I V++VA+T I SF P Y+ I LR+LRF + A +FGLYGV++ +L+ +HL+ L+S Sbjct: 423 IMVIVVAVTAISSFTIPQYSVGITLRILRFIAMFCAAIFGLYGVILFFLLLCSHLVKLKS 482 Query: 472 FAVPYMSPYIPGKWRDWKDSLVRVPLWWMRSRPSFLHTRDNTR 514 F VPY SP +P ++ DWKD +VR+PL MR RP +HT+ N R Sbjct: 483 FGVPYTSPAVPYRFSDWKDFMVRMPLKIMRRRPKIMHTKKNIR 525 >B5UM88.1 B5UM88_BACCE Spore germination protein GerAA Length = 524 Score = 416 bits (1068), Expect = e-114, Method: Compositional matrix adjust. Identities = 219/523 (41%), Positives = 322/523 (61%), Gaps = 10/523 (1%) Query: 1 MQAWK--EKYV--SSIQKQKQPSPAPPEPGKEKLSLSLSANLERLLNRLGQSSDLVVRDF 56 M WK +K V ++IQ + + P + + ++NLE + ++G +SD+ R F Sbjct: 1 MSWWKSRDKQVKKNNIQSKTKQEHKPSQNANVHFTNDFASNLELIKQQIGHNSDVRFRKF 60 Query: 57 LLFGR--YRAALVFFSSLVNQEQIQEHILKPLMAR-SDDHPGLPDNPAGL--SHYIWNTA 111 + GR +A +VF L ++E I +HI+K LM SD++ G YI N Sbjct: 61 NI-GRTDIQAGIVFVDGLSDKEIIDKHIMKLLMDDFSDEYKNESSYVEGTVSKEYIKNKV 119 Query: 112 LQVTQGTTEEDLSALPSGIVKGQLILLIEGSAEALVLDMRQVDTRGVEQPQTEQVIRGPR 171 L +++ + L ++ G LLI+G A+ +L + + R +E+P +E ++RGPR Sbjct: 120 LTISEVAEVHYIKDLIPKVLIGSTALLIDGLADVFILGTTKANKRNIEEPVSEALVRGPR 179 Query: 172 EGYVEKLENNLSLLRYRLQSTDFRIEISPIGSRTQSRVALCYLDSLADPKLVAEVMRRIS 231 G+ E L +N SLLR + + P+G R + + + ++ LA +LV E+ RI Sbjct: 180 VGFTEVLSDNTSLLRRHCADENLSLVKLPVGERAKKELVVAFIKDLASTELVDEIKNRIQ 239 Query: 232 MIQTDGIIDAGYIEQFIEDQPLSPFPQVQSTERPDKTVAGLLEGRVAILVDGSPFALIVP 291 I D ++++GYIEQ IED LSPFPQ+Q+TERPD+ ++ L+EGRVAIL+DG+PF LI P Sbjct: 240 KIDIDSVLESGYIEQLIEDNYLSPFPQIQNTERPDRVISALMEGRVAILLDGTPFVLIAP 299 Query: 292 ALFNQFFQTVDDYTERFIMGXXXXXXXXXXXXXXXXFPALYVSVISFNPELMPTDFAVAI 351 F+ Q+ +DY ER++ G PALY+S ISF+P L+PT A++I Sbjct: 300 VTFSMLLQSPEDYYERWLPGTLLRLLRFMTAFVSLFAPALYISFISFHPGLIPTKLAISI 359 Query: 352 SGGRAGVPFPAVLEVLIMEVSMEVLREATIRLPQMIGGALSIVGVLVIGQAAVAAGLASP 411 G R GVPFPA++E LIMEV++EVLREA +RLP+ IG A+ IVG L+IGQAAV AG+ SP Sbjct: 360 IGSREGVPFPALIEALIMEVAIEVLREAGLRLPKPIGPAMGIVGGLIIGQAAVEAGIVSP 419 Query: 412 ITVVIVALTTIGSFATPAYNAAIALRMLRFPLILLAGMFGLYGVMIGTILIINHLLFLES 471 I V++VA+T I SF P Y+ I LR+LRF + A +FGLYGV++ +L+ +HL+ L+S Sbjct: 420 IMVIVVAVTAISSFTIPQYSVGITLRILRFIAMFCAAIFGLYGVILFFLLLCSHLVKLKS 479 Query: 472 FAVPYMSPYIPGKWRDWKDSLVRVPLWWMRSRPSFLHTRDNTR 514 F VPY SP +P ++ DWKD +VR+PL MR RP +HT+ N R Sbjct: 480 FGVPYTSPAVPYRFSDWKDFIVRMPLKIMRRRPKIMHTKKNIR 522 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,173,416,076 Number of extensions: 129580449 Number of successful extensions: 373899 Number of sequences better than 1.0e-05: 1091 Number of HSP's gapped: 371590 Number of HSP's successfully gapped: 1095 Length of query: 551 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 410 Effective length of database: 1,732,333,088 Effective search space: 710256566080 Effective search space used: 710256566080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)