BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0035.p (334 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DG94.1 B1DG94_9BACL Spore germination protein 205 4e-51 B3K5F1.1 B3K5F1_9BACI Spore germination protein 196 3e-48 C0ZJ03.1 C0ZJ03_BREBN Spore germination protein 108 5e-22 Q65IQ0.1 Q65IQ0_BACLD Spore germination protein 108 1e-21 C0Z9D9.1 C0Z9D9_BREBN Spore germination protein 106 4e-21 >B1DG94.1 B1DG94_9BACL Spore germination protein Length = 373 Score = 205 bits (522), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 112/330 (33%), Positives = 186/330 (56%), Gaps = 3/330 (0%) Query: 2 ADAGGSSAPLVAFTGVLISFFSFWLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWL 61 AD+GG PLV F +L++F +L+A L RFP E++ +S ++IG+ L+ +L I L Sbjct: 35 ADSGG---PLVTFLAMLLAFVGLFLIARLGIRFPDETIIHYSEKIIGKWLSWIGSLSIIL 91 Query: 62 IFIMLTGLTARQFGDVATSVLFNKTPIEATVVLMLLICQLSARRNIIKFSYIHFFYXXXX 121 F +LT LTAR+FG+V + + TP+E TV+ MLL+ +S R I F++IH FY Sbjct: 92 FFAVLTSLTAREFGEVVVTSVLKSTPVEVTVIAMLLLAAISTRHGITTFAHIHTFYFPFL 151 Query: 122 XXXXXXXXXXSMRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFIITLLVPFMQLPKQ 181 S+++ + +NL P+ KG L + LFQ +F++T ++P M+ P + Sbjct: 152 LVPVLVIAALSLKNAEAINLLPIWGNEPGGMLKGTLTIAALFQGAFVLTAVIPNMKHPGK 211 Query: 182 AVRXXXXXXXXXXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALF 241 A++ +Y++IV A++ +FG EE K L++PTLE A++ + LER+DA F Sbjct: 212 AMKASFWGMLIAGGLYVIIVAATISVFGSEEIKKLLWPTLELAKATSLPANILERLDASF 271 Query: 242 IVIWVISVXXXXXXXXXXXXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNVFETYTWT 301 + +WV +V + +L ++ ++ S +LP VF +AMLPKN+ + Y Sbjct: 272 LAVWVTAVFTTLFSSYFFTIYSISHLFRLKEHKLFSYFILPFVFVMAMLPKNIIQMYEII 331 Query: 302 LLLGKMSMILMVGYPALLWAVYGVRRFRKK 331 +G++ +I+ + YP LL V +R+ R Sbjct: 332 ENVGRIGLIITIVYPGLLLIVSILRKKRGN 361 >B3K5F1.1 B3K5F1_9BACI Spore germination protein Length = 364 Score = 196 bits (497), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 110/328 (33%), Positives = 179/328 (54%), Gaps = 3/328 (0%) Query: 2 ADAGGSSAPLVAFTGVLISFFSFWLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWL 61 ADAG AP V F G+L++F + + L RFP +S +S +IG+ +LI Sbjct: 36 ADAG---APFVTFLGMLLAFVGLFFMTRLGMRFPNDSYVEYSEVIIGKWFGRIGSLISIA 92 Query: 62 IFIMLTGLTARQFGDVATSVLFNKTPIEATVVLMLLICQLSARRNIIKFSYIHFFYXXXX 121 F +L+ L AR+FG+V + + TP+E TV++MLL+ S+RRNI+ FSYIH FY Sbjct: 93 FFAVLSSLVAREFGEVVVTAVLKNTPLEITVLVMLLLAAFSSRRNIMTFSYIHLFYSPFI 152 Query: 122 XXXXXXXXXXSMRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFIITLLVPFMQLPKQ 181 S+++ DL+N+ P+ G + S +FQ FI+ +++P M P++ Sbjct: 153 LIPALLIVALSLKNADLVNVLPLWGNEPGGIPAGIITLSTVFQGYFIMMMVIPAMCKPER 212 Query: 182 AVRXXXXXXXXXXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALF 241 A+R +Y+LIV A+VG+FG EETK L++PTLE A++ + LER+DA+F Sbjct: 213 AMRSSLWAMVVTGALYILIVAATVGVFGAEETKKLLWPTLELAKATSLPANVLERLDAVF 272 Query: 242 IVIWVISVXXXXXXXXXXXXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNVFETYTWT 301 + +WV +V + L D M S +LP +F +AMLP+++ + Sbjct: 273 LAVWVTAVFTSLFSCYYFTLYSISKLFRLADHGMLSFFILPFLFVLAMLPQSLIQLNEVN 332 Query: 302 LLLGKMSMILMVGYPALLWAVYGVRRFR 329 ++ K +++ + YP +L + +R+ R Sbjct: 333 GMISKYGLLITIAYPGILLIIAILRKKR 360 >C0ZJ03.1 C0ZJ03_BREBN Spore germination protein Length = 368 Score = 108 bits (271), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 79/313 (25%), Positives = 145/313 (46%), Gaps = 12/313 (3%) Query: 25 WLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIMLTGLTARQFGDVATSVLFN 84 WLLA L RFPR+S F +S + +P+A T ++ + ++ R G++A LF Sbjct: 61 WLLAKLAARFPRQSFFEYSSIIASKPIAYILTFLVCIYTMLFVSFEIRAIGNIAKQYLFY 120 Query: 85 KTPIEATVVLMLLICQLSARRNIIKFSYIHFFYXXXXXXXXXXXXXXSMRDVDLLNLQPV 144 TP+E + LLI Q S + I ++ + + + D+ N++P Sbjct: 121 NTPVEMITLSFLLIVQYSVIGSRIAMLRLNLLFLPVVLVVMFVVLLFTTQLFDIQNVRPF 180 Query: 145 LTAPSASFWKGALEASYLFQSSF----IITLLVPFMQLPKQAVRXXXXXXXXXXXVYLLI 200 ++ W+ LE S + S+ II M+ P++ + +Y+ I Sbjct: 181 FSSD----WRSLLEGSRVVGLSYSGFEIILFYTMLMKKPQEGAKAVTLGLSIPILLYMTI 236 Query: 201 VIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALFIVIWVISVXXXXXXXXXXX 260 + ++G+F E L YPT+E A+ + GF ER++++F IW++++ Sbjct: 237 YMFAIGVFSAEVATNLTYPTIELAKEVEIPGGFFERVESVFFTIWIMTIFNTCAMWLDIT 296 Query: 261 XXLLQNLLAFRDQRMTSSVLL--PLVFAIAMLPKNVFETYTWTLLLGKMSMILMVGYPAL 318 L ++ F R T VL+ P+++ +AMLP+N+ + +T+ + MIL+ P L Sbjct: 297 VLNLSSM--FNKVRKTFWVLILSPMIYFVAMLPQNLVDFFTFADNITYFGMILVYLCPIL 354 Query: 319 LWAVYGVRRFRKK 331 L + +R + Sbjct: 355 LLLIAVIRGVKGH 367 >Q65IQ0.1 Q65IQ0_BACLD Spore germination protein Length = 366 Score = 108 bits (269), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 88/320 (27%), Positives = 151/320 (47%), Gaps = 6/320 (1%) Query: 12 VAFTGVLISFFSFWLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIMLTGLTA 71 VAF G L++ + +++ L +P ++L+ +SR LIG+ FTL++ F+ +G Sbjct: 47 VAFGG-LVAILAGFIMVKLSMEYPDKTLYQYSRDLIGKWPGGLFTLLLICYFLATSGFQV 105 Query: 72 RQFGDVATSVLFNKTPIEATVVLMLL--ICQLSARRNIIKFSYIHFFYXXXXXXXXXXXX 129 R +V L +TPI AT ++ L I L+ N + Y F Sbjct: 106 RSVTEVMGFYLLEETPIWATAMVFLWSGIYLLTGGINPLARLYEIIFPITVLIFLIVIFM 165 Query: 130 XXSMRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFIITLLVPFMQLPKQAVRXXXXX 189 S+ D+D NL+PVL +G S+ + II ++VPFM+ P+QA++ Sbjct: 166 SASIFDID--NLRPVLGKGIKPVLEGVKTTSFAYTGIEIILIIVPFMRKPEQALKATLMG 223 Query: 190 XXXXXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALFIVIWVISV 249 Y++ VI +G +E +PT++ RS + ER ++L +VIW++ + Sbjct: 224 ISFPIVFYMITVIMVIGALAIEGVTSRTWPTIDLMRSFEIPGLIFERFESLLLVIWIMQM 283 Query: 250 XXXXXXXXXXXXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNVFETYTWTLLLGKMSM 309 L LL R + + S LLP+++ IAM PKN+ + + L+G ++ Sbjct: 284 FSSFTISYFAAALGLSQLLKKRMEPILFS-LLPVIYLIAMAPKNINQLFQMGDLIGNSAI 342 Query: 310 ILMVGYPALLWAVYGVRRFR 329 L+ P +L + VRR + Sbjct: 343 YLVGLIPLMLLVISKVRRKK 362 >C0Z9D9.1 C0Z9D9_BREBN Spore germination protein Length = 386 Score = 106 bits (264), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 88/340 (25%), Positives = 161/340 (47%), Gaps = 29/340 (8%) Query: 5 GGSSAPLVAFTGVLISFFSFWLLAALCRRFPRESLFVFSRRLIG---RP-LAMFFTL--- 57 G APL+ G ++ S WL+ L +R+P E+ +S ++ G RP + F+L Sbjct: 45 AGWIAPLIGSAG---AYVSLWLIVKLSKRYPGETFIEYSHKVWGSAKRPWVGKVFSLPWI 101 Query: 58 IIWLIFIML-TGLTARQFGDVATSVLFNKTPIEATVV----LMLLIC----QLSARRNII 108 I+L+++ T + +R FG+V + + +TP+E ++ L+L +C ++ AR N + Sbjct: 102 CIYLVYLYFSTAVVSRVFGEVVVTSVLLQTPLEVMLITMFLLILFLCMHEVEVVARVNEL 161 Query: 109 KFSYIHFFYXXXXXXXXXXXXXXSMRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFI 168 F +I F S + ++ NL P+ S +G E Y + I Sbjct: 162 LFPFIIF--------PLLFIAIASFQKAEIGNLLPINHVSIRSMVEGVYEGIYSYSGFEI 213 Query: 169 ITLLVPFMQLPKQAVRXXXXXXXXXXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAV 228 + + + Q +Y+LIV+A + +FG EE + + +PTLE ++ Sbjct: 214 MFIFFAYAQQNTNRELAGFYSLLIAASLYMLIVVAGISVFGYEELQRVTWPTLELVKTTQ 273 Query: 229 VGDGFLERMDALFIVIWVISVXXXXXXXXXXXXXLLQNLL--AFRDQRMTSSVLLPLVFA 286 V LER+++ F+ +WV +V L+ L R QR+ +SVLL +F Sbjct: 274 VPGLILERLESAFLAVWVSAVFTTVANAYYVVVYSLRQLFRKGIRFQRIMASVLLIPLFF 333 Query: 287 IAMLPKNVFETYTWTLLLGKMSMILMVGYPALLWAVYGVR 326 A++P+N+ E ++ +LG S+++ + P + W +R Sbjct: 334 FALIPQNITEVFSMASVLGLSSLVINLLIPVVYWVAILIR 373 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.333 0.144 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,561,521,833 Number of extensions: 51355197 Number of successful extensions: 186604 Number of sequences better than 1.0e-05: 257 Number of HSP's gapped: 186302 Number of HSP's successfully gapped: 257 Length of query: 334 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 198 Effective length of database: 1,779,438,163 Effective search space: 352328756274 Effective search space used: 352328756274 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 131 (55.1 bits)