BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0034.p (406 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5F3.1 B3K5F3_9BACI Germination protein, Ger(X)C family 341 7e-92 B1DG95.1 B1DG95_9BACL Germination protein, Ger(X)C family 337 1e-90 C0Z9E0.1 C0Z9E0_BREBN Spore germination protein 159 7e-37 B8CZP5.1 B8CZP5_HALOH Spore germination B3 GerAC family protein 137 2e-30 B4BBF1.1 B4BBF1_CLOTM Germination protein, Ger(X)C family 130 3e-28 >B3K5F3.1 B3K5F3_9BACI Germination protein, Ger(X)C family Length = 411 Score = 341 bits (875), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 169/386 (43%), Positives = 247/386 (63%), Gaps = 4/386 (1%) Query: 23 TGCWDQVEIEDRALVLGLSIDTVPPEKADQEEEVTHLLDT-PVPE-EMISVTAQIAVPGR 80 TGCWD++EIE+RA+VLG+SIDT + +E+E++HL P P+ EMI ++ QIA+PGR Sbjct: 24 TGCWDRMEIEERAVVLGISIDTAGKDGEIREDEISHLRGKYPAPQQEMIQLSVQIALPGR 83 Query: 81 VPLGPGSGSSEDGKTSP-VWVVTVTGHSLDDAMNNLQQQIADPRYLVHLRVIVISEDIAR 139 +PLGPG G + VWV+ V GH++DDAM NLQQQI+ + HLRVIV+SE+ AR Sbjct: 84 IPLGPGEGGASGEGAQETVWVLDVVGHTVDDAMMNLQQQISGKLFFGHLRVIVVSEEFAR 143 Query: 140 GRMDDLNDYLRRNPEVRRRTWLLVSEGRASQFMDVNPPLQRVPTLYILSMMEKAVTSGKF 199 M++LNDYL RN EVRR WL+VS+G+A + M+ P L+RVP LY+ S ++ AV GKF Sbjct: 144 KGMENLNDYLHRNAEVRRMAWLMVSKGKARELMEAAPKLERVPALYLSSTLDDAVKHGKF 203 Query: 200 PPDYIGTYWSADSKWGQSAYLPYVALRNKDNVLINGLAYFSQGKMVNTTKPLEIGAFMAV 259 P DYIGT+W+ SK GQ +LPY+ + DNV I+G+A+F +V TKP EI ++ + Sbjct: 204 PSDYIGTFWNNSSKKGQEGFLPYIQIMKGDNVEISGMAFFKGATLVGVTKPFEIAGYLVI 263 Query: 260 QGMDPGGYSAFFETRELGVVMT-KTNKRYTKTRSSIRDGKPVLTYDIYLEGDLDEHFHSA 318 +G+ P GY + V+T R ++ + I++G P T E +++E Sbjct: 264 KGISPAGYRGIIHVGDDSQVVTIHATNRESEIKVDIKNGLPHFTITAVTEVNVEEKNTET 323 Query: 319 KPADSPASLHEIEQEFNKNVQTLIHNLIRQTQKDHADIFGMGEIIRAHHPAYWKEHIHDK 378 P ++ L EI +E ++V+ L+ LI++TQK +DIFG GE++RA P+YW H+ Sbjct: 324 LPLNNSHILEEIARENERSVKELMLGLIQKTQKKESDIFGFGELVRARKPSYWNSHVKTA 383 Query: 379 DDWEKLYDSVTVNINLALHLRRVGLK 404 D W ++Y T + + +RRVG+K Sbjct: 384 DQWSEIYKHTTFDFRVTSKVRRVGMK 409 >B1DG95.1 B1DG95_9BACL Germination protein, Ger(X)C family Length = 414 Score = 337 bits (864), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 172/412 (41%), Positives = 262/412 (63%), Gaps = 12/412 (2%) Query: 2 NRLLLKISSGLLAAAVISLFLTGCWDQVEIEDRALVLGLSIDTVP-PEKADQEEEVTHLL 60 R +K+ L A + + L GCWD++EIE+RA+VLG+SID V E ++E+ ++HL Sbjct: 4 TRKWIKLVRTLCAVMLATPILAGCWDRLEIEERAVVLGVSIDKVKMEEAEEEEDAISHLP 63 Query: 61 DT-PVP-EEMISVTAQIAVPGRVPLGPGSGSSEDGKT--SPVWVVTVTGHSLDDAMNNLQ 116 T P P ++MI V QIA+PGR+PLGPG G + VWV+ V GHSL+DA NLQ Sbjct: 64 GTIPAPNKDMIRVAVQIALPGRIPLGPGESGGGGGGSVGKTVWVIDVVGHSLNDAFMNLQ 123 Query: 117 QQIADPRYLVHLRVIVISEDIARGRMDDLNDYLRRNPEVRRRTWLLVSEGRASQFMDVNP 176 QQ++ + HLRVIVISE +AR ++++NDY RRN EVRR W++V++G+A + M +P Sbjct: 124 QQVSSKLFFGHLRVIVISEAVARNGLENINDYFRRNSEVRRMAWMMVAKGQARKLMLASP 183 Query: 177 PLQRVPTLYILSMMEKAVTSGKFPPDYIGTYWSADSKWGQSAYLPYVALRNKDNVLINGL 236 PL+RVPTLY++S +++ + GKFP DYIG +WS SK GQ +LPYV ++ + NV I GL Sbjct: 184 PLERVPTLYLMSTLDEGIRMGKFPKDYIGIFWSNVSKKGQEGFLPYVEIKKEQNVEIKGL 243 Query: 237 AYFSQGKMVNTTKPLEIGAFMAVQGMDPGGYSAFFETRELG----VVMTKTNKRYTKTRS 292 AYF MV T+PLEI ++A++G++P GY F +LG +MT R +K + Sbjct: 244 AYFVGDHMVGVTRPLEIAGYLAIKGINPAGYRVFV---QLGSPEQTIMTTATHRRSKIKV 300 Query: 293 SIRDGKPVLTYDIYLEGDLDEHFHSAKPADSPASLHEIEQEFNKNVQTLIHNLIRQTQKD 352 I+D P T DI +E +++ ++P L IE++ + +++I+++Q+ Sbjct: 301 QIKDDVPHFTIDIGIELNVEGKVSDKIQINNPDVLKLIEEKQGSDAIKFYYDIIKESQEK 360 Query: 353 HADIFGMGEIIRAHHPAYWKEHIHDKDDWEKLYDSVTVNINLALHLRRVGLK 404 +D FG GEI+R P YW + I ++W K+Y++ T + +A+ +RR+G+K Sbjct: 361 GSDYFGFGEIVRGRKPQYWNKRIKTAEEWSKMYENCTFEVKVAMKVRRIGMK 412 >C0Z9E0.1 C0Z9E0_BREBN Spore germination protein Length = 376 Score = 159 bits (401), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 107/404 (26%), Positives = 194/404 (48%), Gaps = 58/404 (14%) Query: 17 VISLFLTGCWDQVEIEDRALVLGLSIDTVPPEKADQEEEVTHLLDTPVPEEMISVTAQIA 76 + ++ LTGCWD+ E+E+RA VL ++ID Q+E+ E + +T QI Sbjct: 13 ICTILLTGCWDRRELEERASVLAIAID--------QDEK---------NENLYKMTVQIP 55 Query: 77 VPGRVPLGPGSGSSEDGKTSPVWVVTVTGHSLDDAMNNLQQQIADPRYLVHLRVIVISED 136 +P + + SG V +++VTG ++ DA NNLQ ++ +L H R++ +SE+ Sbjct: 56 IP--IKIAGSSGKGGGNNADAVKIMSVTGRTVTDASNNLQMRLNQRLFLGHTRILAVSEE 113 Query: 137 IARGRMDDLNDYLRRNPEVRRRTWLLVSEGRASQFMDVNPPLQRVPTLYILSMMEKAVTS 196 +AR + D+ D RR P++RR W +V G+AS +++ P ++ +P ++++ ++E + Sbjct: 114 VARKGIQDIMDSFRREPQIRRLLWPIVVRGKASTLLEIKPRIEPIPVVFLMGLIENGMKL 173 Query: 197 GKFPPDYIGTYWSADSKWGQSAYLPYVALRNKDNVLINGLAYFSQGKMVNTTKPLEIGAF 256 G+ P +G Y++ S +L YV RN + V G+A F KMV ++ Sbjct: 174 GRIPDQTLGDYFNQTSNLTMEPFLNYVEARN-NEVSWKGVAVFRGHKMVGILDHVQSWVL 232 Query: 257 MAVQGMDPGGYSAFFETRELGVVMTKTNKRYTKTRSSIRDGKPVL------TYDIYLEGD 310 + ++ PGG ++ + + KT Y R K ++ TY L+GD Sbjct: 233 LQLRNKKPGG--------DIVIPLPKTKNGYVSFRPHFVKKKVIIHKSSSATYQCELQGD 284 Query: 311 LDEHFHSAKPADSPASLHEIEQEFNKNVQTLIHNLIRQTQKD---------HADIFGMGE 361 + E + K ++F +Q LI + K ++DI +G Sbjct: 285 IVELTENLKIP---------PEQFILQMQALIKKEMENRAKKLLQQLQKQYNSDILKLGL 335 Query: 362 IIRA-HHPAYWKEHIHDKDDWEKLYDSVTVNINLALHLRRVGLK 404 +RA H+ YWK H +W++ + + + + LRR+G++ Sbjct: 336 TLRAKHYQDYWKTH-----NWKQDFKDFPIRVTYTIKLRRLGME 374 >B8CZP5.1 B8CZP5_HALOH Spore germination B3 GerAC family protein Length = 391 Score = 137 bits (345), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 99/405 (24%), Positives = 188/405 (46%), Gaps = 31/405 (7%) Query: 7 KISSGLLAAAVISLFLTGCWDQVEIEDRALVLGLSIDTVPPEKADQEEEVTHLLDTPVPE 66 +I ++ +IS+ LTGCWD +I++RA VL + ID D P P Sbjct: 4 RILPAIMVIVLISILLTGCWDLKDIDERATVLAIGIDYA---------------DIPQPG 48 Query: 67 E-----MISVTAQIAVPGRVPLGPGSGSSEDGKTSPVWVVTVTGHSLDDAMNNLQQQIAD 121 M+ VT QIA+ + + G+G + VW ++ TG ++ A+ Q+++ Sbjct: 49 NFEGSYMVKVTLQIAISEQ--MTGGAGQAPPMGEQVVWNISSTGATMARAIELCQEKLKY 106 Query: 122 PRYLVHLRVIVISEDIARGRMDDLNDYLRRNPEVRRRTWLLVSEGRASQFMDVNPPLQRV 181 + H+R+++ + +A+ ++ +Y R +P RR +++LVS+G A+ + P + Sbjct: 107 SLFFGHVRILIFGQKVAQNGVNQFLNYFRNHPMFRRLSYILVSKGDAADVLKTFPKSATI 166 Query: 182 PTLYILSMMEKAVTSGKFPPDYIGTYWSADSKWGQSAYLPYVALRNKDNVLINGLAYFSQ 241 +++++M+E A +G+ P + G V+ +KD+V+ G+A F Sbjct: 167 QAMFLMNMVETAQNTGRMPDIPFMEFVVRLIDKGIDPVAIMVS-SHKDSVVNTGVAVFRG 225 Query: 242 GKMVNTTKPLEIGAFMAVQGMDPGGYSAFFETR-ELGVVMTKTNKRYTKTRSSI-RDGKP 299 MV + E F+ + GG + ELG V + + R I ++GK Sbjct: 226 DNMVGSLDLYETWNFIQITENQRGGIEVVRDVEDELGRVTIQLTGVNSNVRPIIQKNGKV 285 Query: 300 VLTYDIYLEGDLDEHFHSAKPADSPASLHEIEQEFNKNVQTLIHNLIRQTQKDH-ADIFG 358 D+ +EG + + + P +E+ K + + +I + QK ADIFG Sbjct: 286 KFIIDMEVEGRIVSQ-ETQTDYNDPVLFSALEERVEKEYKRELEQMINKVQKQFKADIFG 344 Query: 359 MGEIIRAHHPAYWKEHIHDKDDWEKLYDSVTVNINLALHLRRVGL 403 G+ +RA+ WKE ++W K + +++ + + +RR+G+ Sbjct: 345 FGQQLRAYQNREWKEI----NNWRKAFSEGELDLTIYVDIRRMGM 385 >B4BBF1.1 B4BBF1_CLOTM Germination protein, Ger(X)C family Length = 423 Score = 130 bits (327), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 103/416 (24%), Positives = 195/416 (46%), Gaps = 36/416 (8%) Query: 12 LLAAAVISLFLTGCWDQVEIEDRALVLGLSID-------------TVPPEKADQEEEVTH 58 L+ +++ LTGCWD ++IEDRA V+G++ID +P + ++ E + Sbjct: 16 LIIIPSLTILLTGCWDSIDIEDRAYVIGIAIDEYPQLPQGIKNKENIPENEQERTFESST 75 Query: 59 LLDTPVPEEMISVTAQIAVPGRVPLGPGSGSSEDGKTSPVWVVTVTGHSLDDAMNNLQQQ 118 +DT VP +++ I +P G+SE T W +T G+S + ++ + Sbjct: 76 EVDTGVPSYAMTIQIPIIKHASLPNILSGGTSEPN-TLKTWDITQVGNSFMEINRSITTR 134 Query: 119 IADPRYLVHLRVIVISEDIARGRMDDLNDYLRRNPEVRRRTWLLVSEGRASQFMDVNPPL 178 + HL+VI+ISE +AR + ++ D R+ E+R RT L +++G A + +DV P + Sbjct: 135 MNLIPNYEHLQVIIISEKVARKGLRNVLDLFIRDHEMRSRTKLFITDGDAKKALDVIPRI 194 Query: 179 QRVPTLYILSMMEKAVTSGKFPPDYIGTYWSADSKWGQSAY------LPYVALRNKDNVL 232 + ++Y+ M A +G+ +W + Q+ Y LP + + + V+ Sbjct: 195 EDYASIYLTKMPRSARVNGEI------LHWMDLGQAVQAIYSGEDFELPALEVTEYE-VM 247 Query: 233 INGLAYFSQGKMVN--TTKPLEIGAFM---AVQGMDPGGYSAFFETRELGVVMTKTNKRY 287 G A F KMV K +EI M + G+ + + E GV+ + K Sbjct: 248 NKGAALFKNDKMVGWADGKDVEIIKIMHNVLLGGIFTSKFVSDEHDSENGVMSLEIIKSK 307 Query: 288 TKTRSSIRDGKPVLTYDIYLEGDL-DEHFHSAKPADSPASLHEIEQEFNKNVQTLIHNLI 346 TK I+D ++ ++G+ D H + + E+ F ++++ I Sbjct: 308 TKITPVIQDDDITFKINVDIKGNYSDSVNHPLTEKIDKDFIEKAEEAFEESIKEQCIKTI 367 Query: 347 RQTQKDHADIFGMGEIIRAHHPAYWKEHIHDKDDWEKLYDSVTVNINLALHLRRVG 402 ++ Q D F G +IR+ P++W + KD W++++ V +N+ +++R++G Sbjct: 368 KKMQDLGVDTFHFGTVIRSKKPSHWSKI---KDRWDEIFPEVKTEVNVKVNIRQIG 420 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,551,615,594 Number of extensions: 111380087 Number of successful extensions: 272142 Number of sequences better than 1.0e-05: 455 Number of HSP's gapped: 271554 Number of HSP's successfully gapped: 466 Length of query: 406 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 268 Effective length of database: 1,760,596,133 Effective search space: 471839763644 Effective search space used: 471839763644 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)