BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0032.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DJK9.1 B1DJK9_9BACL Pseudouridine synthase EC=5.4.99.- 387 e-105 A8U9H8.1 A8U9H8_9LACT Pseudouridine synthase EC=5.4.99.- 344 8e-93 Q8CVA8.1 Q8CVA8_OCEIH Pseudouridine synthase EC=5.4.99.- 339 2e-91 C4CYI9.1 C4CYI9_9SPHI Pseudouridine synthase, RluA family 318 3e-85 A6LIG6.1 A6LIG6_PARD8 Pseudouridine synthase EC=5.4.99.- 281 5e-74 >B1DJK9.1 B1DJK9_9BACL Pseudouridine synthase EC=5.4.99.- Length = 322 Score = 387 bits (993), Expect = e-105, Method: Compositional matrix adjust. Identities = 184/281 (65%), Positives = 229/281 (81%), Gaps = 1/281 (0%) Query: 33 KSYTVAEPAELLAFLLKTITSRGRNSIKSMLSRGQVSVNGKAVTKHNFQLHPGQTVTIDL 92 KSY VAE +ELLAFLL + ++GRNS+KS+L+ GQV VN + TK+N+ L PG+TV I Sbjct: 25 KSYAVAEASELLAFLLAHL-NQGRNSVKSLLAHGQVYVNDRVETKYNYPLQPGETVVIRK 83 Query: 93 EKPVQAKEMTGLTIVYEDDDLIVIQKEAGLLSIATGEDNELTAYRQLMEHVRQSNPHNRV 152 E+ + + GLTI++ED+ +IV KEAGLL+IA+ ++ ++TAYRQLM +VR+S+P NR+ Sbjct: 84 EREKEKFPLIGLTILHEDEHVIVASKEAGLLTIASDKETDMTAYRQLMAYVRESDPKNRI 143 Query: 153 FVVHRLDRDTSGVMMFAKSEQLQQTLQNTWKETVKERSYVALVEGVVKKPEGTISSWLKE 212 F+VHRLDRDTSGV+MFAKSE +QQ LQ W+ VKERSYVALVEG VK+ EGTISSWLKE Sbjct: 144 FIVHRLDRDTSGVLMFAKSEAVQQKLQEEWQTIVKERSYVALVEGEVKRQEGTISSWLKE 203 Query: 213 TSTLKMYSSPHEGDGLHAVTHYKLIQGNRHFSLLEVHLETGRKNQIRVHMSDIGHPIVGD 272 T TL+MYSSPH DG HAVTHYKL+Q + FSL+EVHLETGRKNQIRVHM DIGHPI GD Sbjct: 204 TKTLRMYSSPHPNDGQHAVTHYKLLQAGKVFSLVEVHLETGRKNQIRVHMQDIGHPIAGD 263 Query: 273 KKYGAETKAVGRLGLHARLLSFVHPSSGELLTFESAIPKTF 313 KKYG+ TK +GRLGLHAR+L+F HP +G+LL F++ IPK F Sbjct: 264 KKYGSTTKPIGRLGLHARVLAFEHPVTGKLLKFDTGIPKLF 304 >A8U9H8.1 A8U9H8_9LACT Pseudouridine synthase EC=5.4.99.- Length = 309 Score = 344 bits (882), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 163/284 (57%), Positives = 212/284 (74%), Gaps = 1/284 (0%) Query: 35 YTVAEPAELLAFLLKTITSRGRNSIKSMLSRGQVSVNGKAVTKHNFQLHPGQTVTIDLEK 94 YTV EP ELL FLL +T RNS+KS+L+RGQV+V+ K KHNF L PGQ V I K Sbjct: 25 YTVTEPMELLPFLLSVMTHTSRNSVKSILTRGQVTVDEKMTKKHNFPLLPGQVVAIQSNK 84 Query: 95 P-VQAKEMTGLTIVYEDDDLIVIQKEAGLLSIATGEDNELTAYRQLMEHVRQSNPHNRVF 153 ++ + G+TI+YEDD +IVI KEAGLLSIA ELTA+RQL+ +V+Q N +NR++ Sbjct: 85 AAIKETALIGMTILYEDDAIIVIDKEAGLLSIAAKFAQELTAHRQLINYVKQENQNNRIY 144 Query: 154 VVHRLDRDTSGVMMFAKSEQLQQTLQNTWKETVKERSYVALVEGVVKKPEGTISSWLKET 213 VVHRLDRDTSGVM+FAK+E+++Q +Q WKE VKER Y ALVEGVV++ EGTI +WL E+ Sbjct: 145 VVHRLDRDTSGVMVFAKTEKIKQDMQENWKEIVKERIYTALVEGVVREDEGTIKTWLTES 204 Query: 214 STLKMYSSPHEGDGLHAVTHYKLIQGNRHFSLLEVHLETGRKNQIRVHMSDIGHPIVGDK 273 +K++S+ ++ G A+THYK I+ N F+LLE LETGRKNQIRVHM +GHP+ GDK Sbjct: 205 KAMKVHSATYDNGGKLAITHYKKIRNNEDFTLLEARLETGRKNQIRVHMETLGHPVAGDK 264 Query: 274 KYGAETKAVGRLGLHARLLSFVHPSSGELLTFESAIPKTFLKYS 317 KYGA + + RLGLHA L+ +HP++GEL+ F S +PK FL +S Sbjct: 265 KYGATSNPLKRLGLHATTLALIHPTTGELVRFHSKVPKAFLMHS 308 >Q8CVA8.1 Q8CVA8_OCEIH Pseudouridine synthase EC=5.4.99.- Length = 303 Score = 339 bits (870), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 163/284 (57%), Positives = 218/284 (76%), Gaps = 2/284 (0%) Query: 34 SYTVAEPAELLAFLLKTITSRGRNSIKSMLSRGQVSVNGKAVTKHNFQLHPGQTVTIDLE 93 +Y V E +LL FLLK + +R RNS+KS+L+RGQVSV+ TKHNF L+ G TV I Sbjct: 17 TYEVEETEQLLPFLLKVMDNRSRNSVKSILTRGQVSVDDHIETKHNFMLNKGSTVRILKN 76 Query: 94 KP-VQAKEMTGLTIVYEDDDLIVIQKEAGLLSIATGEDNELTAYRQLMEHVRQSNPHNRV 152 K +Q G++I+YEDDD+IVI+KE+GLLSIAT ++ + TA QLM +V+Q +P NRV Sbjct: 77 KAAIQQDVFVGMSIMYEDDDIIVIEKESGLLSIATEKERKHTALHQLMHYVKQQHPANRV 136 Query: 153 FVVHRLDRDTSGVMMFAKSEQLQQTLQNTWKETVKERSYVALVEGVVKKPEGTISSWLKE 212 +VVHRLD+DTSGVMMFAK+E++++ LQN WK+ VKER+YVALVEG V K +G I SWLKE Sbjct: 137 YVVHRLDKDTSGVMMFAKNERVKRKLQNNWKDIVKERTYVALVEGQVAKEKGYIVSWLKE 196 Query: 213 TSTLKMYSSPHEGDGLHAVTHYKLIQGNRHFSLLEVHLETGRKNQIRVHMSDIGHPIVGD 272 ++T KMYSS + DG A T Y+++Q N +SLLE+ LETGRKNQIRVHM ++GHP+VGD Sbjct: 197 SATRKMYSSRVKNDGQRAATRYRVLQTNNRYSLLELQLETGRKNQIRVHMEELGHPVVGD 256 Query: 273 KKYGAETKAV-GRLGLHARLLSFVHPSSGELLTFESAIPKTFLK 315 K+YG++ ++V GRLGLHA++LSF HP + +L+ F S +P F + Sbjct: 257 KRYGSKGRSVIGRLGLHAKILSFYHPVTNKLMLFRSDVPNAFFQ 300 >C4CYI9.1 C4CYI9_9SPHI Pseudouridine synthase, RluA family Length = 308 Score = 318 bits (816), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 147/278 (52%), Positives = 209/278 (75%) Query: 37 VAEPAELLAFLLKTITSRGRNSIKSMLSRGQVSVNGKAVTKHNFQLHPGQTVTIDLEKPV 96 V+EPAEL+AFL+ + + RN+IKS+LS Q+ V+GK T+ N L PGQ+VT+ + Sbjct: 22 VSEPAELMAFLIAQLPHKNRNNIKSLLSNKQILVDGKVFTQFNHPLKPGQSVTVAANRAP 81 Query: 97 QAKEMTGLTIVYEDDDLIVIQKEAGLLSIATGEDNELTAYRQLMEHVRQSNPHNRVFVVH 156 Q + GLTI+YED LIVI K+AGLLS+AT ++ + TAY L E+ ++ NP N++F++H Sbjct: 82 QTSQYRGLTILYEDQFLIVINKQAGLLSMATNKERDRTAYGILSEYTKKENPKNKIFIIH 141 Query: 157 RLDRDTSGVMMFAKSEQLQQTLQNTWKETVKERSYVALVEGVVKKPEGTISSWLKETSTL 216 RLDR+TSGVMMFAKSE++Q+ +Q +W T KER+YVALVEG+ + P+GTI+S+L+E+ L Sbjct: 142 RLDRETSGVMMFAKSEKVQRLMQESWNATTKERTYVALVEGIPEPPQGTITSYLRESKAL 201 Query: 217 KMYSSPHEGDGLHAVTHYKLIQGNRHFSLLEVHLETGRKNQIRVHMSDIGHPIVGDKKYG 276 +YSS + G ++T+Y + + ++LLE+ LETGRKNQIRVHM DIGHP+ GD+KYG Sbjct: 202 IVYSSQNPDTGQLSITNYTVTKTGNGYALLELELETGRKNQIRVHMQDIGHPVAGDEKYG 261 Query: 277 AETKAVGRLGLHARLLSFVHPSSGELLTFESAIPKTFL 314 AE+ +GRLGLHA +L+F HP +G + F++ IPK+FL Sbjct: 262 AESDPIGRLGLHAEVLAFEHPITGVPMRFDAPIPKSFL 299 >A6LIG6.1 A6LIG6_PARD8 Pseudouridine synthase EC=5.4.99.- Length = 304 Score = 281 bits (719), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 136/281 (48%), Positives = 191/281 (67%), Gaps = 1/281 (0%) Query: 33 KSYTVAEPAELLAFLLKTITSRGRNSIKSMLSRGQVSVNGKAVTKHNFQLHPGQTVTIDL 92 + TV E LL FL + + + R+S+K +LSRGQ+SVNGK + + L+P TV I+ Sbjct: 20 RQVTVGESNTLLPFLFELLKEQSRSSVKGLLSRGQISVNGKVTRQFDAPLNPNDTVGINY 79 Query: 93 EKPVQAKEMTGLTIVYEDDDLIVIQKEAGLLSIATGEDNELTAYRQLMEHVRQSNPHNRV 152 + L IV+EDDDLIVI K GLLS++T E TAYR L +++++ +P N++ Sbjct: 80 GRGKVEFNNPLLRIVWEDDDLIVINKREGLLSVSTDRIKERTAYRILSDYLKECDPRNKI 139 Query: 153 FVVHRLDRDTSGVMMFAKSEQLQQTLQNTWKETVKERSYVALVEGVVKKPEGTISSWLKE 212 FV+HRLDRDTSG+MMFAK++++++ LQ+ W E + +R+YVA++EG +K I S L E Sbjct: 140 FVLHRLDRDTSGIMMFAKNQKVKEQLQSNWSEAITQRTYVAVIEGRPEKDTDLIVSNLVE 199 Query: 213 TSTLKMYSSPHEGDGLHAVTHYKLIQGNRHFSLLEVHLETGRKNQIRVHMSDIGHPIVGD 272 +++Y + +GDG A+T Y+L+Q N +SL+E+ LETGRKNQIR M IGHPI GD Sbjct: 200 NKHMQVYVT-QDGDGKEAITRYRLLQTNNRYSLVELDLETGRKNQIRAQMQSIGHPIAGD 258 Query: 273 KKYGAETKAVGRLGLHARLLSFVHPSSGELLTFESAIPKTF 313 KYGAET GRL LHAR L F+HP +GE + FE+ IP+ F Sbjct: 259 SKYGAETNPAGRLMLHARRLCFIHPVTGEEMRFETRIPEKF 299 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.130 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,749,906,054 Number of extensions: 69300652 Number of successful extensions: 193295 Number of sequences better than 1.0e-05: 4881 Number of HSP's gapped: 182041 Number of HSP's successfully gapped: 4894 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)