BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0023.p (190 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DFC3.1 B1DFC3_9BACL Transcriptional regulator, TetR family 242 1e-62 B3KEA5.1 B3KEA5_9BACI Regulatory protein TetR 188 2e-46 C2W4W4.1 C2W4W4_BACCE Putative uncharacterized protein 166 9e-40 A7GM10.1 A7GM10_BACCN Transcriptional regulator, TetR family 162 1e-38 A8FBM8.1 A8FBM8_BACP2 Possible TetR/AcrR family transcriptional ... 160 6e-38 >B1DFC3.1 B1DFC3_9BACL Transcriptional regulator, TetR family Length = 198 Score = 242 bits (617), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 119/193 (61%), Positives = 147/193 (76%), Gaps = 4/193 (2%) Query: 1 MSIDRKALILQAATKSFVQFGYKATTMDQVSKIANVGKGTIYXXXXXXXXXXXXILDKAS 60 MSIDRKALIL+AAT+SF QFGYKATTMD VSKIA+VGKGTIY IL+KA Sbjct: 1 MSIDRKALILKAATQSFAQFGYKATTMDLVSKIASVGKGTIYTFFKTKEELFEAILNKAE 60 Query: 61 QELTSVMN---RLAAEDNTFIQKLFNLLDSILEFRSDHELFVKLAQEVRDIGTVQALEGV 117 EL MN + D +FI +F+LLDSILEFRSDHELFVKLAQEVRDIGT QALEG+ Sbjct: 61 VELKEAMNCGFDMEGSDRSFIHSVFSLLDSILEFRSDHELFVKLAQEVRDIGTAQALEGI 120 Query: 118 KRMEEYALDFLRYQIEQAMERGEVKPCDASVAAFMIFRLYLALTTEWNKEH-EPLDKNRI 176 +RME + L+FL+ ++E A+E+GEV+ CD ++ ++MIFRLYL L TEWNK H EPL K +I Sbjct: 121 RRMELFGLEFLKQKLELAIEKGEVRECDTTITSYMIFRLYLGLATEWNKSHSEPLTKEQI 180 Query: 177 KEHMILFISNGIL 189 KE++ + I+ GI+ Sbjct: 181 KENIAMLITKGIM 193 >B3KEA5.1 B3KEA5_9BACI Regulatory protein TetR Length = 193 Score = 188 bits (477), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 90/187 (48%), Positives = 134/187 (71%) Query: 2 SIDRKALILQAATKSFVQFGYKATTMDQVSKIANVGKGTIYXXXXXXXXXXXXILDKASQ 61 ++DR+ +L AA+KSF FGYKATTM+QV+KIANVGKGTIY IL A Sbjct: 3 AVDRRKQVLDAASKSFALFGYKATTMEQVAKIANVGKGTIYTFFTNKDELFDEILLSAIM 62 Query: 62 ELTSVMNRLAAEDNTFIQKLFNLLDSILEFRSDHELFVKLAQEVRDIGTVQALEGVKRME 121 E+ + R +D F L+ +LD++LEFRS+HELF+KL+QEVRDIGT +A EG+ ++E Sbjct: 63 EMKRLAEREIRKDRDFFDNLYRVLDALLEFRSEHELFIKLSQEVRDIGTPKAHEGLTKVE 122 Query: 122 EYALDFLRYQIEQAMERGEVKPCDASVAAFMIFRLYLALTTEWNKEHEPLDKNRIKEHMI 181 + +L+ +E A+ GE+KP DA V +F++ +LY+ALTT++N+ H+PL K +IKE+M+ Sbjct: 123 NGIVGYLKGIVENAISIGEIKPLDAQVVSFVMLKLYVALTTDFNQLHKPLSKEQIKEYMM 182 Query: 182 LFISNGI 188 +F+++G+ Sbjct: 183 VFLADGL 189 >C2W4W4.1 C2W4W4_BACCE Putative uncharacterized protein Length = 190 Score = 166 bits (420), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 83/188 (44%), Positives = 119/188 (63%) Query: 1 MSIDRKALILQAATKSFVQFGYKATTMDQVSKIANVGKGTIYXXXXXXXXXXXXILDKAS 60 M+IDRK I++AATKSF FGYKATTMDQV+K+ANVGKGTIY I+ Sbjct: 1 MAIDRKRSIIEAATKSFSAFGYKATTMDQVAKLANVGKGTIYTFFKNKEELFGEIISNLI 60 Query: 61 QELTSVMNRLAAEDNTFIQKLFNLLDSILEFRSDHELFVKLAQEVRDIGTVQALEGVKRM 120 E+ V D +F + + L SILEFR +H+L +KL QE RD+GT + EG++++ Sbjct: 61 VEMKQVAKEAIHSDVSFFENVHKALYSILEFRKEHQLMIKLIQEERDMGTKEVQEGMQQV 120 Query: 121 EEYALDFLRYQIEQAMERGEVKPCDASVAAFMIFRLYLALTTEWNKEHEPLDKNRIKEHM 180 E + ++ +E A+E+GE+ CD + AF++ RLY++L +W K H+PLDK RI E Sbjct: 121 ETEIVSVIQSYLEVAIEKGEISKCDPEITAFIMLRLYVSLIFDWEKNHKPLDKERIAELF 180 Query: 181 ILFISNGI 188 L++ G+ Sbjct: 181 ELYLLKGL 188 >A7GM10.1 A7GM10_BACCN Transcriptional regulator, TetR family Length = 190 Score = 162 bits (411), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 82/188 (43%), Positives = 119/188 (63%) Query: 1 MSIDRKALILQAATKSFVQFGYKATTMDQVSKIANVGKGTIYXXXXXXXXXXXXILDKAS 60 M+IDRK I++AATKSF FGYKATTMDQV+K+ANVGKGTIY I+ Sbjct: 1 MAIDRKRSIIEAATKSFSAFGYKATTMDQVAKLANVGKGTIYTFFKNKEELFGEIISNLI 60 Query: 61 QELTSVMNRLAAEDNTFIQKLFNLLDSILEFRSDHELFVKLAQEVRDIGTVQALEGVKRM 120 E+ V D +F + + L SILEFR +H+L +KL QE RD+GT + E ++++ Sbjct: 61 AEMKEVAKESIRPDISFFENVHRALYSILEFRKEHQLMIKLIQEERDMGTKEVQEVMQQV 120 Query: 121 EEYALDFLRYQIEQAMERGEVKPCDASVAAFMIFRLYLALTTEWNKEHEPLDKNRIKEHM 180 + + F++ +E A+E+GE+ CD + AF++ RLY++L +W K HEPL+K RI E Sbjct: 121 DTEIVSFIQAYLEFAIEKGEISKCDPEITAFIMLRLYVSLIFDWEKNHEPLEKERIAELF 180 Query: 181 ILFISNGI 188 L++ G+ Sbjct: 181 ELYLLKGL 188 >A8FBM8.1 A8FBM8_BACP2 Possible TetR/AcrR family transcriptional regulator Length = 192 Score = 160 bits (404), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 75/188 (39%), Positives = 121/188 (64%) Query: 1 MSIDRKALILQAATKSFVQFGYKATTMDQVSKIANVGKGTIYXXXXXXXXXXXXILDKAS 60 MS+DR+ +IL ATK+F QFGYKATTMD V+K+ANVGKGTIY I Sbjct: 1 MSVDRRQMILDGATKAFTQFGYKATTMDLVAKLANVGKGTIYTFFKNKEELFDEIFTSLL 60 Query: 61 QELTSVMNRLAAEDNTFIQKLFNLLDSILEFRSDHELFVKLAQEVRDIGTVQALEGVKRM 120 E+ + + E N+F + L L +ILEFR +H+L +K+ QE ++GT E +++M Sbjct: 61 LEMRKIADEAIDEKNSFSENLHLALFAILEFRKNHQLTIKIFQENAELGTSAVKEMIEKM 120 Query: 121 EEYALDFLRYQIEQAMERGEVKPCDASVAAFMIFRLYLALTTEWNKEHEPLDKNRIKEHM 180 E+ + +++ ++++AM++G++KPCD + AF++ +LY+AL +W K +EPL K + E + Sbjct: 121 EQMIIRYIKTKVQEAMDKGDIKPCDPELTAFVMVKLYIALIFDWEKRYEPLTKEEVAESL 180 Query: 181 ILFISNGI 188 L++ G+ Sbjct: 181 ELYVLKGL 188 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 836,231,716 Number of extensions: 26337438 Number of successful extensions: 81115 Number of sequences better than 1.0e-05: 144 Number of HSP's gapped: 81311 Number of HSP's successfully gapped: 144 Length of query: 190 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 62 Effective length of database: 1,854,806,283 Effective search space: 114997989546 Effective search space used: 114997989546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)