BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0022.p (369 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3U5.1 B3K3U5_9BACI ApbE family lipoprotein 322 5e-86 Q8R7D3.1 Q8R7D3_THETN Membrane-associated lipoprotein involved i... 280 2e-73 B7R655.1 B7R655_9THEO ApbE family protein 280 2e-73 B1KUH9.1 B1KUH9_CLOBM ApbE family protein 271 8e-71 B0KBX3.1 B0KBX3_THEP3 ApbE family lipoprotein 271 8e-71 >B3K3U5.1 B3K3U5_9BACI ApbE family lipoprotein Length = 354 Score = 322 bits (824), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 156/349 (44%), Positives = 233/349 (66%), Gaps = 6/349 (1%) Query: 24 ACGSSKDKNSSPEETAGGAKVSTD----GGTKSLEQTFYIYDTVVNIKVFGDKAEQKNMD 79 +CGS + A G D T+ + ++I+DT+V+++V+ ++ ++ D Sbjct: 7 SCGSLLIMLTLLMTGAIGCTTKADQKTAASTEPATERYFIFDTIVSLRVYDERMTTRHFD 66 Query: 80 DIQQMLERMDKEFSRTKEDGELYAVNKAAGKEAVAVSDETLDIVKQSLKYAEEMDGLFDP 139 +++ +LER+D+ +R E+ VN+AAGK V+VS+ET +V+ ++ YA G F+P Sbjct: 67 EVRALLERIDQRMNRQLAGSEIDQVNQAAGKSEVSVSEETFKVVQTAIDYAAASGGHFEP 126 Query: 140 TIGPLVDLWNIGSGGESVPPQAEIDKAKALTNYKDVIVDEAAKTVKLAKEGMVLDMGGIG 199 T+GPLVDLW IG +VP + E+ A NYKDVI++ A+ ++L KEGM LD+G I Sbjct: 127 TVGPLVDLWGIGGEHPAVPKKDELTAAMQRMNYKDVILNPASNAIRLEKEGMSLDLGAIA 186 Query: 200 KGYAADRIADYLKEQGLDSAMINLGGSSIIALGNKPNGSPWNIGLQDPDQSRGSQLGTIK 259 KGYAAD IADYL++QG SA+I+LGG +I+A+G+KP+GSPWNIG+QDP++ RG LGT++ Sbjct: 187 KGYAADVIADYLQQQGFRSAIIDLGG-NILAMGSKPDGSPWNIGIQDPEKDRGQSLGTLE 245 Query: 260 ISDEVIDASGVYERFFMQDGVRYHHILDPRTGFPSQNGLKSITIMSPNATDADALSTGVF 319 + ++ I SGVYERFF +G YHHIL P TG+P N L S+TI++ + DADA+ST VF Sbjct: 246 VVNKTIVTSGVYERFFEAEGTVYHHILSPFTGYPVDNDLLSVTIVTDTSMDADAMSTSVF 305 Query: 320 LMGLEDGMKYLESLPEKVDAFFITDDNKIYATSDLKKRLQLTDPTYTFA 368 +GL +G +++ES + DA F+T D++IY TS L +LT+ Y A Sbjct: 306 SLGLTEGRQFVES-RDDADAIFVTTDHQIYLTSGLTDTFKLTNDNYQLA 353 >Q8R7D3.1 Q8R7D3_THETN Membrane-associated lipoprotein involved in thiamine biosynthesis Length = 341 Score = 280 bits (715), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 136/323 (42%), Positives = 215/323 (66%), Gaps = 4/323 (1%) Query: 45 STDGGTKSLEQTFYIYDTVVNIKVFGDKAEQKNMDDIQQMLERMDKEFSRTKEDGELYAV 104 ++ ++ + T + DT + V+ DK +K +DD + +E ++K+ S T ED E+ + Sbjct: 20 CSNKNSQPISDTKFALDTYCTLTVY-DKVPKKVLDDGFKAIEDIEKKMSVTIEDSEVSEI 78 Query: 105 NKAAGKEAVAVSDETLDIVKQSLKYAEEMDGLFDPTIGPLVDLWNIGSGGESVPPQAEID 164 NK AG V VS +T ++K+++ ++E G FD T+GP+V+LWNIG+ VP EI Sbjct: 79 NKNAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVNLWNIGTDKARVPSAEEIK 138 Query: 165 KAKALTNYKDVIVDEAAKTVKLAKEGMVLDMGGIGKGYAADRIADYLKEQGLDSAMINLG 224 L NYK++++DE TV L +GM +D+GGI KGYAAD++A+ LK++G+ A+INLG Sbjct: 139 AKLPLVNYKNIVLDEKNMTVMLKDKGMSIDLGGIAKGYAADKVAEVLKKEGVKHAIINLG 198 Query: 225 GSSIIALGNKPNGSPWNIGLQDPDQSRGSQLGTIKISDEVIDASGVYERFFMQDGVRYHH 284 G ++ +G KP+G+ W +G+Q P + RG G +++ ++ + SG+YER+F +DG YHH Sbjct: 199 G-DVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVTSGIYERYFEKDGKLYHH 257 Query: 285 ILDPRTGFPSQNGLKSITIMSPNATDADALSTGVFLMGLEDGMKYLESLPEKVDAFFITD 344 IL+P TG+P+ N L S+TI++ + D DAL+ +F+MGL++G+K E+LP V+A F+TD Sbjct: 258 ILNPFTGYPADNHLYSVTIITDTSIDGDALAK-IFVMGLDEGLKVAENLP-GVEAIFVTD 315 Query: 345 DNKIYATSDLKKRLQLTDPTYTF 367 D K+Y TS LK ++TDP YT Sbjct: 316 DKKVYITSGLKGNFRITDPEYTL 338 >B7R655.1 B7R655_9THEO ApbE family protein Length = 341 Score = 280 bits (715), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 136/323 (42%), Positives = 215/323 (66%), Gaps = 4/323 (1%) Query: 45 STDGGTKSLEQTFYIYDTVVNIKVFGDKAEQKNMDDIQQMLERMDKEFSRTKEDGELYAV 104 ++ ++ + T + DT + V+ DK +K +DD + +E ++K+ S T ED E+ + Sbjct: 20 CSNKNSQPISDTKFALDTYCTLTVY-DKVPKKVLDDGFKAIEDIEKKMSVTIEDSEVSEI 78 Query: 105 NKAAGKEAVAVSDETLDIVKQSLKYAEEMDGLFDPTIGPLVDLWNIGSGGESVPPQAEID 164 NK AG V VS +T ++K+++ ++E G FD T+GP+V+LWNIG+ VP EI Sbjct: 79 NKNAGIRPVKVSPDTFYVIKKAIHFSEIEKGYFDITVGPIVNLWNIGTDKARVPSAEEIK 138 Query: 165 KAKALTNYKDVIVDEAAKTVKLAKEGMVLDMGGIGKGYAADRIADYLKEQGLDSAMINLG 224 L NYK++++DE TV L +GM +D+GGI KGYAAD++A+ LK++G+ A+INLG Sbjct: 139 AKLPLVNYKNIVLDEKNMTVMLKDKGMSIDLGGIAKGYAADKVAEVLKKEGVKHAIINLG 198 Query: 225 GSSIIALGNKPNGSPWNIGLQDPDQSRGSQLGTIKISDEVIDASGVYERFFMQDGVRYHH 284 G ++ +G KP+G+ W +G+Q P + RG G +++ ++ + SG+YER+F +DG YHH Sbjct: 199 G-DVLTIGTKPDGTNWRVGIQTPFKPRGEYFGIVEVGEKAVVTSGIYERYFEKDGKLYHH 257 Query: 285 ILDPRTGFPSQNGLKSITIMSPNATDADALSTGVFLMGLEDGMKYLESLPEKVDAFFITD 344 IL+P TG+P+ N L S+TI++ + D DAL+ +F+MGL++G+K E+LP V+A F+TD Sbjct: 258 ILNPFTGYPADNHLYSVTIITDTSIDGDALAK-IFVMGLDEGLKVAENLP-GVEAIFVTD 315 Query: 345 DNKIYATSDLKKRLQLTDPTYTF 367 D K+Y TS LK ++TDP YT Sbjct: 316 DKKVYITSGLKGNFRITDPEYTL 338 >B1KUH9.1 B1KUH9_CLOBM ApbE family protein Length = 344 Score = 271 bits (693), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 143/341 (41%), Positives = 208/341 (60%), Gaps = 23/341 (6%) Query: 32 NSSPEETAGGAKVSTDGGTKSLEQTFYIYDTVVNIKVFG---DKAEQKNMDDIQQMLERM 88 NS PEE + + Y+ T++NIK +G DKA Q ++D I + +M Sbjct: 23 NSKPEE--------------PVSRETYLMGTIINIKAYGKNADKAVQSSVDKISDIENKM 68 Query: 89 DKEFSRTKEDGELYAVNKAAGKEAVAVSDETLDIVKQSLKYAEEMDGLFDPTIGPLVDLW 148 S E+ +NK AG V VS T D++K SL Y+E+ G FD T+ PLV LW Sbjct: 69 ----SLNIPTSEVNKINKNAGIAPVKVSKNTFDVIKASLIYSEKSKGSFDITVEPLVSLW 124 Query: 149 NIGSGGESVPPQAEIDKAKALTNYKDVIVDEAAKTVKLAKEGMVLDMGGIGKGYAADRIA 208 IG+ +P + EI A L NYKDVI++E TV L ++G +D+G I KGYAAD + Sbjct: 125 GIGTDKARIPSKDEISNALKLINYKDVIINEKESTVMLKRKGQAIDLGAIAKGYAADELK 184 Query: 209 DYLKEQGLDSAMINLGGSSIIALGNKPNGSPWNIGLQDPDQSRGSQLGTIKISDEVIDAS 268 + L + SA ++LGG+ + LGNKP+ +PW IG+Q+P + RG LG + +SD+ + S Sbjct: 185 EVLLNHNISSAFLSLGGN-VYVLGNKPDKTPWKIGVQNPLEPRGDYLGIVSVSDKSVVTS 243 Query: 269 GVYERFFMQDGVRYHHILDPRTGFPSQNGLKSITIMSPNATDADALSTGVFLMGLEDGMK 328 G YERFF ++G RYHHI D +TG+P++ GL S++I+S + D DALST V+ +GL++G K Sbjct: 244 GNYERFFEKNGKRYHHIFDTKTGYPAEKGLISVSIISDKSIDGDALSTSVYTLGLDEGKK 303 Query: 329 YLESLPEKVDAFFITDDNKIYATSDLKKRLQLTDPTYTFAN 369 +ESL + V+A F+T+D K+Y TS LK +LT+ + N Sbjct: 304 LIESLKD-VEAVFVTNDKKVYVTSGLKDIFKLTNTDFKLQN 343 >B0KBX3.1 B0KBX3_THEP3 ApbE family lipoprotein Length = 345 Score = 271 bits (693), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 135/323 (41%), Positives = 210/323 (65%), Gaps = 4/323 (1%) Query: 45 STDGGTKSLEQTFYIYDTVVNIKVFGDKAEQKNMDDIQQMLERMDKEFSRTKEDGELYAV 104 ++ ++ + T + DT I ++ D +K +D+ + ++ ++ + S T ++ E+ + Sbjct: 24 CSNKNSQPISDTQFALDTYCTISIY-DNVPKKVLDEGFKAIKDVEDKMSVTIKNSEVNQI 82 Query: 105 NKAAGKEAVAVSDETLDIVKQSLKYAEEMDGLFDPTIGPLVDLWNIGSGGESVPPQAEID 164 N+ AG V VS +T ++K+++ +++ G FD T+GP+V LWNIG+ VP EI Sbjct: 83 NENAGIRPVKVSPDTFYVIKKAVHFSDVEKGYFDITVGPIVKLWNIGTDKAKVPSPEEIK 142 Query: 165 KAKALTNYKDVIVDEAAKTVKLAKEGMVLDMGGIGKGYAADRIADYLKEQGLDSAMINLG 224 L NYKD+I+DE T+ L +GM +D+GGI KGYAAD++A LK++G+ A+INLG Sbjct: 143 AKLPLVNYKDIILDEKNMTIMLKNKGMSIDLGGIAKGYAADKVAQVLKKEGVKHAIINLG 202 Query: 225 GSSIIALGNKPNGSPWNIGLQDPDQSRGSQLGTIKISDEVIDASGVYERFFMQDGVRYHH 284 G ++ +G KP+G W IG+Q P + RG LG +++S++ + SGVYER+F +DG YHH Sbjct: 203 G-DVLTIGTKPDGGNWRIGIQTPFKPRGEYLGIVEVSNKAVVTSGVYERYFKKDGKLYHH 261 Query: 285 ILDPRTGFPSQNGLKSITIMSPNATDADALSTGVFLMGLEDGMKYLESLPEKVDAFFITD 344 IL+P TG+P N L S+TI++ + D DALS +F++GLE+G+K ESLP V A F+TD Sbjct: 262 ILNPFTGYPVDNHLYSVTIITDTSIDGDALSK-IFVIGLEEGLKLAESLP-GVQAIFVTD 319 Query: 345 DNKIYATSDLKKRLQLTDPTYTF 367 D K+Y TS LK ++TDP YT Sbjct: 320 DKKVYITSGLKGNFKVTDPEYTL 342 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,159,984,829 Number of extensions: 92754866 Number of successful extensions: 224267 Number of sequences better than 1.0e-05: 1219 Number of HSP's gapped: 221509 Number of HSP's successfully gapped: 1220 Length of query: 369 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 232 Effective length of database: 1,770,017,148 Effective search space: 410643978336 Effective search space used: 410643978336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)