BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0019.p (171 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DEZ3.1 B1DEZ3_9BACL Phosphodiesterase, MJ0936 family 204 2e-51 B3KIT4.1 B3KIT4_9BACI Phosphodiesterase, MJ0936 family 170 4e-41 C0GCV5.1 C0GCV5_9FIRM Phosphodiesterase, MJ0936 family 164 2e-39 B1I1R1.1 B1I1R1_DESAP Phosphodiesterase, MJ0936 family 153 4e-36 A6CHH5.1 A6CHH5_9BACI Putative phosphoesterase 149 1e-34 >B1DEZ3.1 B1DEZ3_9BACL Phosphodiesterase, MJ0936 family Length = 168 Score = 204 bits (520), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 92/167 (55%), Positives = 122/167 (73%) Query: 4 MKVGVVSDTHLSSRAKGLPSVLMEEFSKVDMILHLGDWVSLEIYDLLAELAPVEGIAGNN 63 MK+ VVSDTH+ AK LP L+ E + D+ILH GDW S ++Y LA+ APV+G+AGNN Sbjct: 1 MKIVVVSDTHMPRMAKALPPRLLAELANADLILHAGDWTSSDVYRELAKFAPVKGVAGNN 60 Query: 64 DGAEIIRRFGERKIVTLEGVRIGMIHGHAPYSRKGTDGNALLAFEGEKVDCILFGHSHQP 123 DG I+++ G +KIV G RIG++HGH PYS K + NA L+F +VD I+FGHSH P Sbjct: 61 DGETIVKKLGFKKIVNAGGKRIGLVHGHLPYSGKKAEQNAALSFTAAQVDAIVFGHSHVP 120 Query: 124 LLRQEGDILLFNPGSPTDKRREKQYSFGLMDIEEGKISARHIFYDSR 170 L+++ ILLFNPGS T KR++ QYSFG+M I EG++ ARHIFYD++ Sbjct: 121 YLKEQNGILLFNPGSATAKRKQPQYSFGIMSIIEGELKARHIFYDNK 167 >B3KIT4.1 B3KIT4_9BACI Phosphodiesterase, MJ0936 family Length = 169 Score = 170 bits (430), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 80/169 (47%), Positives = 113/169 (66%), Gaps = 2/169 (1%) Query: 2 KQMKVGVVSDTHLSSRAKGLPSVLMEEFSKVDMILHLGDWVSLEIYDLLAELAPVEGIAG 61 + ++ V+SDTH+ +AK LP L+E+ + ++ILH GDW E+Y+ L++ APVEG+AG Sbjct: 3 RPYRITVLSDTHMPKKAKELPDPLLEDLRQSELILHAGDWSDWELYEQLSQYAPVEGVAG 62 Query: 62 NNDGAEIIRRFGERKIVTLEGVRIGMIHGHAPYSRKGTDGNALLAFEGEKVDCILFGHSH 121 N D A I+ RFG +++V +EG RIG++HGH T A AF +VDCILFGHSH Sbjct: 63 NVDDARIVERFGYKRLVEIEGKRIGIVHGHG--QGGTTPSRARKAFADTEVDCILFGHSH 120 Query: 122 QPLLRQEGDILLFNPGSPTDKRREKQYSFGLMDIEEGKISARHIFYDSR 170 P +LLFNPGS TDKRR+ +YS G ++I +G + A H+FYD + Sbjct: 121 IPFKEDVQGVLLFNPGSATDKRRQSRYSHGRIEILDGNLEAHHVFYDRK 169 >C0GCV5.1 C0GCV5_9FIRM Phosphodiesterase, MJ0936 family Length = 163 Score = 164 bits (416), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 82/164 (50%), Positives = 109/164 (66%), Gaps = 2/164 (1%) Query: 4 MKVGVVSDTHLSSRAKGLPSVLMEEFSKVDMILHLGDWVSLEIYDLLAELAPVEGIAGNN 63 M++GV+SDTH+ +RAK LP VL + F VD+ILH GD V + D L +APVE +AGN Sbjct: 1 MRIGVLSDTHIPARAKHLPPVLFDLFDGVDLILHAGDLVEESVLDDLTAIAPVEAVAGNM 60 Query: 64 DGAEIIRRFGERKIVTLEGVRIGMIHGHAPYSRKGTDGNALLAFEGEKVDCILFGHSHQP 123 D E+ R GE+KI+ L G IG+IHG+ +R T +L AF GE VDC++FGHSHQP Sbjct: 61 DSFEVHERLGEKKILQLAGYNIGLIHGNIGSNRSKTPQRSLEAFAGEAVDCVVFGHSHQP 120 Query: 124 LLRQEGDILLFNPGSPTDKRREKQYSFGLMDIEEGKISARHIFY 167 + +LLFNPGSPTD+RRE ++S G++ + GK I Y Sbjct: 121 YNERVNGVLLFNPGSPTDRRREPRHSCGIITL--GKTIEAEIIY 162 >B1I1R1.1 B1I1R1_DESAP Phosphodiesterase, MJ0936 family Length = 181 Score = 153 bits (387), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 68/155 (43%), Positives = 108/155 (69%), Gaps = 4/155 (2%) Query: 1 LKQMKVGVVSDTHLSSRAKGLPSVLMEEFSKVDMILHLGDWVSLEIYDLLAELAPVEGIA 60 + Q+ +GV++DTH+ +RA+ +P+ ++E F++V++++H GD LE+ D L+ LAPV +A Sbjct: 16 VSQIIIGVIADTHIPTRARTVPTPVLETFARVNLVIHAGDLTELEVLDELSRLAPVVAVA 75 Query: 61 GNNDGAEIIRRFGERKIVTLEGVRIGMIHGHAPYSRKGTDGNALLAFEGEKVDCILFGHS 120 GN D E+ R+ GE +++ LEG RIG++HGH Y G + L AF + CI+FGH+ Sbjct: 76 GNMDSWEVRRKLGETRLLELEGFRIGVLHGHGGYG--GVEARVLAAF--PEAHCIVFGHT 131 Query: 121 HQPLLRQEGDILLFNPGSPTDKRREKQYSFGLMDI 155 H P + G +LLFNPGSPTD+RR+ + S+GL+ + Sbjct: 132 HAPYCERHGGVLLFNPGSPTDRRRQPRASYGLLHL 166 >A6CHH5.1 A6CHH5_9BACI Putative phosphoesterase Length = 188 Score = 149 bits (375), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 73/169 (43%), Positives = 110/169 (65%), Gaps = 4/169 (2%) Query: 2 KQMKVGVVSDTHLSSRAKGLPSVLMEEFSKVDMILHLGDWVSLEIYDLLAELAPVEGIAG 61 + +K+ V+SDTH+ +R + P VL++E D+I+H GDW ++++Y+ L VEG+ G Sbjct: 22 RTLKMIVISDTHMPNRGQEFPPVLIKELKSADLIIHAGDWNTIDVYEKLKGFGRVEGVYG 81 Query: 62 NNDGAEIIRRFGERKIVTLEGVRIGMIHGHAPYSRKGTDGNALLAFEGEKVDCILFGHSH 121 N D EI+ F ++ ++ EG IG++HG K T+ AL AF+ E+ D I+FGHSH Sbjct: 82 NTDQQEILETFPKKMVLNAEGYSIGVVHGDG--KGKTTEKRALEAFD-ERPDIIIFGHSH 138 Query: 122 QPLLRQEGDILLFNPGSPTDKRREKQYSFGLMDIEEGKISARHIFYDSR 170 P R LLFNPGS TDKR++ YSFG+++I E +I ++HIFY S+ Sbjct: 139 IPYARYSQGTLLFNPGSATDKRKQPYYSFGIIEI-ESEIKSQHIFYKSK 186 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,057,409,739 Number of extensions: 43959980 Number of successful extensions: 87659 Number of sequences better than 1.0e-05: 469 Number of HSP's gapped: 87503 Number of HSP's successfully gapped: 469 Length of query: 171 Length of database: 3,060,696,203 Length adjustment: 126 Effective length of query: 45 Effective length of database: 1,873,648,313 Effective search space: 84314174085 Effective search space used: 84314174085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)