BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0012.p (283 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDT7.1 B3KDT7_9BACI Short-chain dehydrogenase/reductase SDR 322 2e-86 C2BZN6.1 C2BZN6_LISGR Short-chain dehydrogenase/reductase SDR 275 3e-72 Q8EQH7.1 Q8EQH7_OCEIH Alcohol dehydrogenase 267 7e-70 Q17QW3.1 Q17QW3_BOVIN Retinol dehydrogenase 14 (All-trans/9-cis/... 223 1e-56 B4MUW3.1 B4MUW3_DROWI GK14728 221 8e-56 >B3KDT7.1 B3KDT7_9BACI Short-chain dehydrogenase/reductase SDR Length = 287 Score = 322 bits (825), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 158/278 (56%), Positives = 203/278 (73%) Query: 3 MVGKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLML 62 M GK V+VTG N+GMGLATT E+A+ GA VIMACRS+ RGE AL +A+++SGS NI LM Sbjct: 4 MTGKIVIVTGANSGMGLATTSELAKSGAHVIMACRSQARGEAALRQAQQESGSSNIELMS 63 Query: 63 CDLASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXX 122 DL SFDS+RAFA E+ A Y LDVL+NNAGVV ++R+LT DG+E GVNH Sbjct: 64 LDLGSFDSIRAFASEYKAKYEQLDVLVNNAGVVTIQRELTKDGFEAMIGVNHLGHFLLTN 123 Query: 123 XXXERLVAAEQGRIVVVASGAYKIGKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAA 182 E L A QGR+V V+SGA+K+G +H +D L +G+N AK YA+SKLANILFT+ELA Sbjct: 124 ELLEPLQRARQGRVVNVSSGAHKVGSIHFDDPNLGKGFNVAKGYAQSKLANILFTKELAR 183 Query: 183 QLRGTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSPFFLTPEQGADTAIWLAAAPEL 242 +L+ T++TVNA+HPGAV TSIGVNR+TGFG++ KLL PFFLTP +GA TAI+LA++PE+ Sbjct: 184 RLQPTRITVNALHPGAVSTSIGVNRDTGFGKAVHKLLRPFFLTPLEGARTAIYLASSPEV 243 Query: 243 RGVTGEYYYRRKIQELSPXXXXXXXXXQLWQWSLEHTG 280 VTGEYY + K + + +LW+WS + G Sbjct: 244 EHVTGEYYVKCKPAKTTEKARDPKLAARLWEWSEQQVG 281 >C2BZN6.1 C2BZN6_LISGR Short-chain dehydrogenase/reductase SDR Length = 281 Score = 275 bits (704), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 137/276 (49%), Positives = 183/276 (66%) Query: 6 KTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLCDL 65 KT L+TG N GMG ATT+ +A++ V+M CRSR RGEEA +A +SGS I L + DL Sbjct: 2 KTALITGANTGMGYATTVALAKQNMRVVMLCRSRERGEEARKKAVSESGSQTIALHIVDL 61 Query: 66 ASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXXXX 125 +SF S+R AE+ YPV+D++INNAGVV K++ T DG+E GVN+ Sbjct: 62 SSFKSIREAAEQLKVLYPVIDIMINNAGVVTTKKEYTKDGFEKMMGVNYLGHFLLTNLLL 121 Query: 126 ERLVAAEQGRIVVVASGAYKIGKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQLR 185 + AA+ GRIVVV+SGAYK L+L+D + ++ K Y RSKLAN+LF RELA +L Sbjct: 122 PNMEAADAGRIVVVSSGAYKFSPLYLDDFNSDQRFSIWKNYGRSKLANLLFARELARRLS 181 Query: 186 GTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSPFFLTPEQGADTAIWLAAAPELRGV 245 T VTVNA+HPGAV TS+GVNR+TGFG+S LL PFF + E+GA+TA++LA + E++ + Sbjct: 182 RTNVTVNALHPGAVATSLGVNRDTGFGKSITALLKPFFRSAEKGAETAVYLATSEEVKDI 241 Query: 246 TGEYYYRRKIQELSPXXXXXXXXXQLWQWSLEHTGL 281 TGEY+Y +KI+ QLWQ S +T L Sbjct: 242 TGEYFYNKKIKATKGEANNLELAEQLWQKSEVYTEL 277 >Q8EQH7.1 Q8EQH7_OCEIH Alcohol dehydrogenase Length = 290 Score = 267 bits (683), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 135/280 (48%), Positives = 176/280 (62%), Gaps = 1/280 (0%) Query: 3 MVGKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLML 62 M KT++VTG N GMGLATTIE+A++G VIMACR RG EA +A +S S NI L Sbjct: 1 MQNKTIIVTGANTGMGLATTIELAKKGIHVIMACRDTKRGNEAKEKAMEESNSTNISLYQ 60 Query: 63 CDLASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXX 122 CDL S +S+ FA++ + +D LINNAGVV+LK T DG+E GVNH Sbjct: 61 CDLGSMESISKFADQIKEDFDKIDGLINNAGVVSLKHTKTEDGFESMTGVNHLGHFLLTH 120 Query: 123 XXXERLVAAEQGRIVVVASGAYKIGKLHLED-HTLSRGYNPAKAYARSKLANILFTRELA 181 + L +EQ RI+ VASGAYK G L D H +R +N K Y +SKL NILFT EL Sbjct: 121 LLLDVLKKSEQARIINVASGAYKAGTLDYNDMHFNNRSFNVIKGYGQSKLCNILFTLELN 180 Query: 182 AQLRGTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSPFFLTPEQGADTAIWLAAAPE 241 L GT V+ A+HPGAV TS+GV+R+TGFG LL PFFLT E+GA+TAI+LA P+ Sbjct: 181 KHLEGTNVSTFALHPGAVSTSLGVDRQTGFGEKVHLLLRPFFLTSEEGAETAIYLATEPK 240 Query: 242 LRGVTGEYYYRRKIQELSPXXXXXXXXXQLWQWSLEHTGL 281 + ++G Y+Y++K+Q +LW WS++ G+ Sbjct: 241 IDHLSGRYFYKKKLQPTKYNKVNEDEAKKLWDWSIKELGM 280 >Q17QW3.1 Q17QW3_BOVIN Retinol dehydrogenase 14 (All-trans/9-cis/11-cis) Length = 336 Score = 223 bits (568), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 130/297 (43%), Positives = 170/297 (57%), Gaps = 15/297 (5%) Query: 2 LMVGKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQ--------- 52 LM GKTVL+TG N+G+G AT E+ R GA VIM CR R R EEA + +R+ Sbjct: 40 LMRGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRERAEEAAGQLRREVCPAGGPDS 99 Query: 53 ----SGSDNIRLMLCDLASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYEL 108 G+ + + DLAS SVR+F +E P LDVLINNAGV T DG+E+ Sbjct: 100 GPNSGGAGELVVKELDLASLSSVRSFCQEMLQEEPRLDVLINNAGVFQCPYMKTEDGFEM 159 Query: 109 DFGVNHXXXXXXXXXXXERLVAAEQGRIVVVASGAYKIGKLHLEDHTLSRGYNPAKAYAR 168 FGVNH L ++ RIVVV+S YK G ++ ED + YN + Y+R Sbjct: 160 QFGVNHLGHFLLTNLLLGLLKSSAPSRIVVVSSKLYKYGDINFEDLNSEQSYNKSFCYSR 219 Query: 169 SKLANILFTRELAAQLRGTQVTVNAVHPGAVGTSIGVNRETG-FGRSFLKLLS-PFFLTP 226 SKLANILFTRELA +L GT VTVN +HPG V T++G + R L+S FF TP Sbjct: 220 SKLANILFTRELARRLEGTSVTVNVLHPGIVRTNLGRHIHIPLLVRPLFNLVSWAFFKTP 279 Query: 227 EQGADTAIWLAAAPELRGVTGEYYYRRKIQELSPXXXXXXXXXQLWQWSLEHTGLLQ 283 E+GA TA++LA++PE+ GV+G Y+ K +EL P +LW S G+L+ Sbjct: 280 EEGAQTAVYLASSPEVEGVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMVGILK 336 >B4MUW3.1 B4MUW3_DROWI GK14728 Length = 292 Score = 221 bits (562), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 124/278 (44%), Positives = 163/278 (58%), Gaps = 2/278 (0%) Query: 4 VGKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLC 63 +GK V+VTG N G+G T E+ARRGA V MACR + E+A E ++ ++NI Sbjct: 16 IGKVVIVTGANTGIGKETVRELARRGATVYMACRDMEKSEKARREIVEETKNENIFTKHL 75 Query: 64 DLASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXX 123 DL+S DS+R F EEF L +LINNAGV+ R+LT DG+E+ GVNH Sbjct: 76 DLSSLDSIRKFVEEFKTEQDQLHILINNAGVMRGPRRLTKDGFEMQIGVNHMGHFLLTNL 135 Query: 124 XXERLVAAEQGRIVVVASGAYKIGKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQ 183 + L AA RIVVV+SG + GK+ D + Y+ AY++SKLANILFTRELA + Sbjct: 136 LLDNLKAAHSSRIVVVSSGVHCFGKIKTTDLNSEKSYSEGGAYSQSKLANILFTRELAKR 195 Query: 184 LRGTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSPFFLTPEQGADTAIWLAAAPELR 243 L GT+VTVNA+HPGAV T +G R GR +LSPF +PE GA T ++ A P+L Sbjct: 196 LEGTRVTVNALHPGAVNTELG--RNWSAGRVLWPILSPFMKSPEGGAQTTLYAALDPQLE 253 Query: 244 GVTGEYYYRRKIQELSPXXXXXXXXXQLWQWSLEHTGL 281 V+G Y+ K +S LW+ S + TGL Sbjct: 254 LVSGLYFGDCKPMNVSKAAKDDKTGKWLWEESEKWTGL 291 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,500,666,319 Number of extensions: 55793865 Number of successful extensions: 221776 Number of sequences better than 1.0e-05: 10915 Number of HSP's gapped: 221321 Number of HSP's successfully gapped: 10922 Length of query: 283 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 149 Effective length of database: 1,798,280,193 Effective search space: 267943748757 Effective search space used: 267943748757 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)