BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0008.p (241 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z7Q4.1 C0Z7Q4_BREBN Conserved hypothetical membrane protein 314 3e-84 B1DFM2.1 B1DFM2_9BACL Conserved hypothetical integral membrane p... 295 3e-78 Q2B437.1 Q2B437_9BACI YpdP 249 1e-64 B4AKQ9.1 B4AKQ9_BACPU Conserved protein YpdP 248 4e-64 A6CRC5.1 A6CRC5_9BACI YpdP 248 5e-64 >C0Z7Q4.1 C0Z7Q4_BREBN Conserved hypothetical membrane protein Length = 226 Score = 314 bits (805), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 145/227 (63%), Positives = 186/227 (81%), Gaps = 1/227 (0%) Query: 1 MFNLLWGILFVVVNFGFYLLCYRLFGKKGLYAWVGVATVIANIQVAKTIAMPFDIVMTLG 60 MFN G++F +VNFG +LLCYRLFG+ GLYAW+G+ATV+ANIQV KTI M F +VMTLG Sbjct: 1 MFNFWIGVIFALVNFGLFLLCYRLFGRMGLYAWIGMATVLANIQVVKTIEM-FGLVMTLG 59 Query: 61 NTMYVTLYMTSDLLNEKYGRAEARHAVWFGFFTLLMTTAIMQMVLLFEPQETDIAQSSLE 120 NT+Y ++Y+ SDLLNEKYG EA+ AVWFGFFTL+ T IMQ+VL+FEPQETDIAQ SL+ Sbjct: 60 NTIYASIYLVSDLLNEKYGEREAKKAVWFGFFTLIAATIIMQLVLVFEPQETDIAQGSLQ 119 Query: 121 TIFGLMPRLALGSLTAYFISQFLDVRLYAWIRKYYSSSRQLWVRSNGSTMVSSFVDTLVF 180 TIFGLMPR+ALGSL AYF+SQF+DV++Y+W++K QLW+R+NGST+ S +D++ F Sbjct: 120 TIFGLMPRVALGSLCAYFVSQFVDVKIYSWLKKKCPGPNQLWIRNNGSTLFSQMLDSVTF 179 Query: 181 CTIAFAGTYDLRVWTEILLTTYVVKFLLTGAGTPILYIARSFKFAED 227 CTIAF G + + VW +ILLTTY++KF+++ A TP+LYIARS K E Sbjct: 180 CTIAFLGEFPMDVWWQILLTTYLIKFVVSAASTPVLYIARSMKVDES 226 >B1DFM2.1 B1DFM2_9BACL Conserved hypothetical integral membrane protein Length = 230 Score = 295 bits (754), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 136/230 (59%), Positives = 182/230 (79%), Gaps = 3/230 (1%) Query: 1 MFNLLWGILFVVVNFGFYLLCYRLFGKKGLYAWVGVATVIANIQVAKTIAMPFDIVMTLG 60 MFNL +G LF++V+F +L+ YRLFG+ G+YAW+G+ATV+AN+QV KT+ MPFDIV+TLG Sbjct: 1 MFNLWFGALFMLVHFALFLISYRLFGRVGIYAWIGMATVLANVQVVKTVEMPFDIVITLG 60 Query: 61 NTMYVTLYMTSDLLNEKYGRAEARHAVWFGFFTLLMTTAIMQMVLLFEPQETDIAQ---S 117 NTMY T+Y+ SDLLNEKYG A+ AVWFGFFTL+ +T IMQM L+F P +++A Sbjct: 61 NTMYGTIYLASDLLNEKYGERAAKKAVWFGFFTLIASTIIMQMALVFNPDSSELAHDTND 120 Query: 118 SLETIFGLMPRLALGSLTAYFISQFLDVRLYAWIRKYYSSSRQLWVRSNGSTMVSSFVDT 177 +L IFGLMP++ALGSL AYF+SQFLDVR+Y+ ++K + + QLW+R+NGST++S FVDT Sbjct: 121 ALHMIFGLMPQIALGSLCAYFVSQFLDVRIYSILKKVFPAPGQLWIRNNGSTVISQFVDT 180 Query: 178 LVFCTIAFAGTYDLRVWTEILLTTYVVKFLLTGAGTPILYIARSFKFAED 227 L+FCTIAFAGT + VW + L+TYV+KF+++ TP LY AR F F E+ Sbjct: 181 LIFCTIAFAGTLEGSVWVSVFLSTYVIKFIVSLFSTPFLYAARRFTFKEE 230 >Q2B437.1 Q2B437_9BACI YpdP Length = 233 Score = 249 bits (637), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 119/236 (50%), Positives = 164/236 (69%), Gaps = 4/236 (1%) Query: 1 MFNLLWGILFVVVNFGFYLLCYRLFGKKGLYAWVGVATVIANIQVAKTIAMPFDIVMTLG 60 MFN +G +F +VNF F LL YR+FGK GL+ W+G++TV+AN+QV KTI + F + TLG Sbjct: 1 MFNEWFGFIFALVNFAFVLLFYRIFGKTGLFVWIGLSTVLANLQVIKTIEI-FGMTATLG 59 Query: 61 NTMYVTLYMTSDLLNEKYGRAEARHAVWFGFFTLLMTTAIMQMVLLFEPQETDIAQSSLE 120 N MY T ++ +D+LNEKYG+ EA+ AVW GF TLL T IMQMVLLF+P E D+AQ +L Sbjct: 60 NAMYGTAFLVTDILNEKYGKEEAKKAVWLGFLTLLSMTVIMQMVLLFKPHEIDMAQGALS 119 Query: 121 TIFGLMPRLALGSLTAYFISQFLDVRLYAWIRKYYSSSRQLWVRSNGSTMVSSFVDTLVF 180 TIF +P++ALGSL AY SQFLDV ++ ++++ + Q W+R+NGST++S F+DTLVF Sbjct: 120 TIFSFLPQIALGSLAAYLASQFLDVYIFQFLKRKFPKDNQFWIRNNGSTIISQFIDTLVF 179 Query: 181 CTIAFAGTYDLRVWTEILLTTYVVKFLLTGAGTPILYIARSFKFAEDEQPVHTSPD 236 IAFAG + W +I +TTY++KF + TP Y+A+ +TS D Sbjct: 180 TGIAFAGVFPFNEWIQIFITTYLLKFFVAILDTPFGYLAKK---MHKTNKANTSID 232 >B4AKQ9.1 B4AKQ9_BACPU Conserved protein YpdP Length = 229 Score = 248 bits (632), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 123/221 (55%), Positives = 157/221 (71%), Gaps = 1/221 (0%) Query: 1 MFNLLWGILFVVVNFGFYLLCYRLFGKKGLYAWVGVATVIANIQVAKTIAMPFDIVMTLG 60 MFN ILF ++NF LL Y+ FGK GL+ W+G ATV AN+QV KT+ + F + TLG Sbjct: 1 MFNEGIWILFAIINFIIVLLFYKGFGKMGLFVWIGFATVAANLQVVKTVEL-FGLTATLG 59 Query: 61 NTMYVTLYMTSDLLNEKYGRAEARHAVWFGFFTLLMTTAIMQMVLLFEPQETDIAQSSLE 120 N +Y +++ +D+LNEKYG+ EAR AVW GF TLL T +MQ LLF P +DI+QSSLE Sbjct: 60 NILYGSVFFATDVLNEKYGQNEARKAVWIGFATLLTLTVVMQEALLFHPAPSDISQSSLE 119 Query: 121 TIFGLMPRLALGSLTAYFISQFLDVRLYAWIRKYYSSSRQLWVRSNGSTMVSSFVDTLVF 180 TIFG MPR+ALGSL AY ISQ LDV +Y++IR+ + S LWVR+ GSTM+S +DTL+F Sbjct: 120 TIFGFMPRVALGSLAAYIISQMLDVYVYSFIRRIFPSDGALWVRNAGSTMISQLLDTLIF 179 Query: 181 CTIAFAGTYDLRVWTEILLTTYVVKFLLTGAGTPILYIARS 221 TIAF GTY VW EI +TTYV+KF++ TP Y A+ Sbjct: 180 TTIAFLGTYPFHVWIEIFITTYVIKFIVAAIATPYAYAAKK 220 >A6CRC5.1 A6CRC5_9BACI YpdP Length = 225 Score = 248 bits (632), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 115/226 (50%), Positives = 164/226 (72%), Gaps = 1/226 (0%) Query: 1 MFNLLWGILFVVVNFGFYLLCYRLFGKKGLYAWVGVATVIANIQVAKTIAMPFDIVMTLG 60 MFN +G++FV+VNF L YR+FGK GL+ W+GV+T++AN+QV KTI + F + TLG Sbjct: 1 MFNEWFGLIFVLVNFILLLAMYRIFGKTGLFVWIGVSTILANLQVIKTIEI-FGLTATLG 59 Query: 61 NTMYVTLYMTSDLLNEKYGRAEARHAVWFGFFTLLMTTAIMQMVLLFEPQETDIAQSSLE 120 N MY T ++ +D+LNEK+G+ EA+ AVW GFFTLL+ T IMQM L+F+P D AQ SLE Sbjct: 60 NAMYGTAFLVTDILNEKHGKKEAQKAVWLGFFTLLIMTVIMQMALVFQPHPDDFAQESLE 119 Query: 121 TIFGLMPRLALGSLTAYFISQFLDVRLYAWIRKYYSSSRQLWVRSNGSTMVSSFVDTLVF 180 TIFG++PR+A+GSL AY +SQF DV ++A+++K + + W+R+NGSTM+S +DTL F Sbjct: 120 TIFGMIPRIAIGSLAAYLVSQFADVYIFAFLKKKFPKDSKFWIRNNGSTMISQLLDTLAF 179 Query: 181 CTIAFAGTYDLRVWTEILLTTYVVKFLLTGAGTPILYIARSFKFAE 226 +IAF G + VW +I +TTY++K+++ TP YIA+ E Sbjct: 180 TSIAFFGAFPFDVWVQIAITTYLLKWIVALLDTPFGYIAKRLHVKE 225 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.139 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,326,284,642 Number of extensions: 48482611 Number of successful extensions: 147468 Number of sequences better than 1.0e-05: 526 Number of HSP's gapped: 147057 Number of HSP's successfully gapped: 526 Length of query: 241 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 109 Effective length of database: 1,817,122,223 Effective search space: 198066322307 Effective search space used: 198066322307 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)