BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0011.p (450 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5ND58.1 B5ND58_SALET Anaerobic C4-dicarboxylate transporter DcuA 359 3e-97 Q8ZLD2.1 Q8ZLD2_SALTY Putative inner membrane protein 358 7e-97 Q57IM3.1 Q57IM3_SALCH Putative inner membrane protein 358 7e-97 B5F9G5.1 B5F9G5_SALA4 Anaerobic C4-dicarboxylate transporter DcuA 358 7e-97 B4TYZ8.1 B4TYZ8_SALSV Anaerobic C4-dicarboxylate transporter DcuA 358 7e-97 >B5ND58.1 B5ND58_SALET Anaerobic C4-dicarboxylate transporter DcuA Length = 439 Score = 359 bits (922), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 194/400 (48%), Positives = 265/400 (66%), Gaps = 1/400 (0%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 MFW E GAR+G VF+G++GG+GV + VFIF + P++PP+DV+LII Sbjct: 1 MFWTELCFILVALMIGARIGGVFLGMVGGLGVGVMVFIFGLTPSTPPIDVILIILSVVLA 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 + LV +AEK+L + P+ IT++AP IC+LFTF+SGTGHVVYSLLP+I+++A Sbjct: 61 AASLQASGGLDLLVKLAEKILRRHPRYITLLAPFICYLFTFMSGTGHVVYSLLPVISEVA 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 DSGIRPERP T SP+SAA AAM+GLMAPLGV + TI+ I +PATL+GV Sbjct: 121 RDSGIRPERPLSISVIASQQAITASPISAAMAAMIGLMAPLGVSISTIMMICVPATLIGV 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVESAPEEQPEKKATPKGAKXXXXXXXXXXXXX 240 + A+ T KGKEL +DPEY RRLAEGL++ A +E T + AK Sbjct: 181 AMGAIATFNKGKELKDDPEYQRRLAEGLIKPAQKESKNTVVTSR-AKLSVALFLTSAIVI 239 Query: 241 XXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRAGA 300 GL P LRP L+M+ I+I ML A LI+++CRP ++ I++GT+FRAGA Sbjct: 240 VLLGLIPALRPMVETAKGLQPLSMSAAIQITMLSFACLIVLLCRPQVDQIISGTVFRAGA 299 Query: 301 LAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTLIM 360 LA+VCAFGLAWMS TF+ G I+ V ++ W++A++MF V+A+ +SQ ATTLI+ Sbjct: 300 LAIVCAFGLAWMSETFVNGHIALIKAEVQTLLQQHTWLIAIMMFFVSAMVSSQAATTLIL 359 Query: 361 IPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD 400 +P+ + L LPAY ++G+W AVN FF+PV+ QCLAA++FD Sbjct: 360 LPLGLALGLPAYALIGSWPAVNGYFFIPVAGQCLAALAFD 399 >Q8ZLD2.1 Q8ZLD2_SALTY Putative inner membrane protein Length = 439 Score = 358 bits (919), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 193/400 (48%), Positives = 265/400 (66%), Gaps = 1/400 (0%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 MFW E GAR+G VF+G++GG+GV + VFIF + P++PP+DV+LII Sbjct: 1 MFWTELCFILVALMIGARIGGVFLGMVGGLGVGVMVFIFGLTPSTPPIDVILIILSVVLA 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 + LV +AEK+L + P+ IT++AP IC++FTF+SGTGHVVYSLLP+I+++A Sbjct: 61 AASLQASGGLDLLVKLAEKILRRHPRYITLLAPFICYIFTFMSGTGHVVYSLLPVISEVA 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 DSGIRPERP T SP+SAA AAM+GLMAPLGV + TI+ I +PATL+GV Sbjct: 121 RDSGIRPERPLSISVIASQQAITASPISAAMAAMIGLMAPLGVSISTIMMICVPATLIGV 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVESAPEEQPEKKATPKGAKXXXXXXXXXXXXX 240 + A+ T KGKEL +DPEY RRLAEGL++ A +E T + AK Sbjct: 181 AMGAIATFNKGKELKDDPEYQRRLAEGLIKPAQKESKNTVVTSR-AKLSVALFLTSAIVI 239 Query: 241 XXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRAGA 300 GL P LRP L+M+ I+I ML A LI+++CRP ++ I++GT+FRAGA Sbjct: 240 VLLGLIPALRPMVETAKGLQPLSMSAAIQITMLSFACLIVLLCRPQVDQIISGTVFRAGA 299 Query: 301 LAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTLIM 360 LA+VCAFGLAWMS TF+ G I+ V ++ W++A++MF V+A+ +SQ ATTLI+ Sbjct: 300 LAIVCAFGLAWMSETFVNGHIALIKAEVQTLLQQHTWLIAIMMFFVSAMVSSQAATTLIL 359 Query: 361 IPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD 400 +P+ + L LPAY ++G+W AVN FF+PV+ QCLAA++FD Sbjct: 360 LPLGLALGLPAYALIGSWPAVNGYFFIPVAGQCLAALAFD 399 >Q57IM3.1 Q57IM3_SALCH Putative inner membrane protein Length = 439 Score = 358 bits (919), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 193/400 (48%), Positives = 265/400 (66%), Gaps = 1/400 (0%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 MFW E GAR+G VF+G++GG+GV + VFIF + P++PP+DV+LII Sbjct: 1 MFWTELCFILVALMIGARIGGVFLGMVGGLGVGVMVFIFGLTPSTPPIDVILIILSVVLA 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 + LV +AEK+L + P+ IT++AP IC++FTF+SGTGHVVYSLLP+I+++A Sbjct: 61 AASLQASGGLDLLVKLAEKILRRHPRYITLLAPFICYIFTFMSGTGHVVYSLLPVISEVA 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 DSGIRPERP T SP+SAA AAM+GLMAPLGV + TI+ I +PATL+GV Sbjct: 121 RDSGIRPERPLSISVIASQQAITASPISAAMAAMIGLMAPLGVSISTIMMICVPATLIGV 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVESAPEEQPEKKATPKGAKXXXXXXXXXXXXX 240 + A+ T KGKEL +DPEY RRLAEGL++ A +E T + AK Sbjct: 181 AMGAIATFNKGKELKDDPEYQRRLAEGLIKPAQKESKNTVVTSR-AKLSVALFLTSAIVI 239 Query: 241 XXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRAGA 300 GL P LRP L+M+ I+I ML A LI+++CRP ++ I++GT+FRAGA Sbjct: 240 VLLGLIPALRPMVETAKGLQPLSMSAAIQITMLSFACLIVLLCRPQVDQIISGTVFRAGA 299 Query: 301 LAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTLIM 360 LA+VCAFGLAWMS TF+ G I+ V ++ W++A++MF V+A+ +SQ ATTLI+ Sbjct: 300 LAIVCAFGLAWMSETFVNGHIALIKAEVQTLLQQHTWLIAIMMFFVSAMVSSQAATTLIL 359 Query: 361 IPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD 400 +P+ + L LPAY ++G+W AVN FF+PV+ QCLAA++FD Sbjct: 360 LPLGLALGLPAYALIGSWPAVNGYFFIPVAGQCLAALAFD 399 >B5F9G5.1 B5F9G5_SALA4 Anaerobic C4-dicarboxylate transporter DcuA Length = 439 Score = 358 bits (919), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 193/400 (48%), Positives = 265/400 (66%), Gaps = 1/400 (0%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 MFW E GAR+G VF+G++GG+GV + VFIF + P++PP+DV+LII Sbjct: 1 MFWTELCFILVALMIGARIGGVFLGMVGGLGVGVMVFIFGLTPSTPPIDVILIILSVVLA 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 + LV +AEK+L + P+ IT++AP IC++FTF+SGTGHVVYSLLP+I+++A Sbjct: 61 AASLQASGGLDLLVKLAEKILRRHPRYITLLAPFICYIFTFMSGTGHVVYSLLPVISEVA 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 DSGIRPERP T SP+SAA AAM+GLMAPLGV + TI+ I +PATL+GV Sbjct: 121 RDSGIRPERPLSISVIASQQAITASPISAAMAAMIGLMAPLGVSISTIMMICVPATLIGV 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVESAPEEQPEKKATPKGAKXXXXXXXXXXXXX 240 + A+ T KGKEL +DPEY RRLAEGL++ A +E T + AK Sbjct: 181 AMGAIATFNKGKELKDDPEYQRRLAEGLIKPAQKESKNTVVTSR-AKLSVALFLTSAIVI 239 Query: 241 XXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRAGA 300 GL P LRP L+M+ I+I ML A LI+++CRP ++ I++GT+FRAGA Sbjct: 240 VLLGLIPALRPMVETAKGLQPLSMSAAIQITMLSFACLIVLLCRPQVDQIISGTVFRAGA 299 Query: 301 LAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTLIM 360 LA+VCAFGLAWMS TF+ G I+ V ++ W++A++MF V+A+ +SQ ATTLI+ Sbjct: 300 LAIVCAFGLAWMSETFVNGHIALIKAEVQTLLQQHTWLIAIMMFFVSAMVSSQAATTLIL 359 Query: 361 IPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD 400 +P+ + L LPAY ++G+W AVN FF+PV+ QCLAA++FD Sbjct: 360 LPLGLALGLPAYALIGSWPAVNGYFFIPVAGQCLAALAFD 399 >B4TYZ8.1 B4TYZ8_SALSV Anaerobic C4-dicarboxylate transporter DcuA Length = 439 Score = 358 bits (919), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 193/400 (48%), Positives = 265/400 (66%), Gaps = 1/400 (0%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 MFW E GAR+G VF+G++GG+GV + VFIF + P++PP+DV+LII Sbjct: 1 MFWTELCFILVALMIGARIGGVFLGMVGGLGVGVMVFIFGLTPSTPPIDVILIILSVVLA 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 + LV +AEK+L + P+ IT++AP IC++FTF+SGTGHVVYSLLP+I+++A Sbjct: 61 AASLQASGGLDLLVKLAEKILRRHPRYITLLAPFICYIFTFMSGTGHVVYSLLPVISEVA 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 DSGIRPERP T SP+SAA AAM+GLMAPLGV + TI+ I +PATL+GV Sbjct: 121 RDSGIRPERPLSISVIASQQAITASPISAAMAAMIGLMAPLGVSISTIMMICVPATLIGV 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVESAPEEQPEKKATPKGAKXXXXXXXXXXXXX 240 + A+ T KGKEL +DPEY RRLAEGL++ A +E T + AK Sbjct: 181 AMGAIATFNKGKELKDDPEYQRRLAEGLIKPAQKESKNTVVTSR-AKLSVALFLTSAIVI 239 Query: 241 XXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRAGA 300 GL P LRP L+M+ I+I ML A LI+++CRP ++ I++GT+FRAGA Sbjct: 240 VLLGLIPALRPMVETAKGLQPLSMSAAIQITMLSFACLIVLLCRPQVDQIISGTVFRAGA 299 Query: 301 LAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTLIM 360 LA+VCAFGLAWMS TF+ G I+ V ++ W++A++MF V+A+ +SQ ATTLI+ Sbjct: 300 LAIVCAFGLAWMSETFVNGHIALIKAEVQTLLQQHTWLIAIMMFFVSAMVSSQAATTLIL 359 Query: 361 IPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD 400 +P+ + L LPAY ++G+W AVN FF+PV+ QCLAA++FD Sbjct: 360 LPLGLALGLPAYALIGSWPAVNGYFFIPVAGQCLAALAFD 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,278,044,117 Number of extensions: 81203424 Number of successful extensions: 305729 Number of sequences better than 1.0e-05: 696 Number of HSP's gapped: 304006 Number of HSP's successfully gapped: 857 Length of query: 450 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 311 Effective length of database: 1,751,175,118 Effective search space: 544615461698 Effective search space used: 544615461698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)