BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0004.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9F3.1 B1D9F3_9BACL Mannonate dehydratase EC=4.2.1.8 508 e-142 A6M2D7.1 A6M2D7_CLOB8 Mannonate dehydratase EC=4.2.1.8 500 e-140 A6M0R4.1 A6M0R4_CLOB8 Mannonate dehydratase EC=4.2.1.8 499 e-139 Q5WKD5.1 UXUA_BACSK Mannonate dehydratase EC=4.2.1.8 497 e-139 B3K847.1 B3K847_9BACI Mannonate dehydratase EC=4.2.1.8 492 e-137 >B1D9F3.1 B1D9F3_9BACL Mannonate dehydratase EC=4.2.1.8 Length = 357 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 236/358 (65%), Positives = 286/358 (79%), Gaps = 2/358 (0%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M MT+RWYGEGND+++L I+QIPGV G+VW+LH+ AGE W M++I E GF Sbjct: 1 MRMTFRWYGEGNDSVSLKQIKQIPGVEGLVWALHDVPAGEEWPMDKIMEYKRLADEHGFH 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVHDDIK+GLP+RD+YI+ Y TI KLA VGVKVICYNFMP+FDW RT+L+KE Sbjct: 61 LEVVESVNVHDDIKLGLPSRDQYIENYKRTIEKLATVGVKVICYNFMPIFDWMRTDLFKE 120 Query: 121 LPDGSNALFYEKAAITDNPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEEK 180 + DGS ALF++KA I E + + G ++MPGWEPERL +L LF AY DVTEE Sbjct: 121 MEDGSTALFFDKAKIQSMSAEELVSNIAGNSSYSMPGWEPERLKRLAPLFEAYKDVTEED 180 Query: 181 LFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYNG 240 LF NLKYFLE+IIPVCE+ DIKMAIHPDDP + +FGLPRI+ SRD + R+L VDSPYNG Sbjct: 181 LFNNLKYFLEQIIPVCEKHDIKMAIHPDDPPYSVFGLPRIVTSRDNLARLLKLVDSPYNG 240 Query: 241 LTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAEV 300 LT C+GSLG+NP+ND+PA++REF DRI FAHIRNV+V+ENGDFIE SH+ DG+VD+AEV Sbjct: 241 LTLCSGSLGANPDNDIPALVREFADRIPFAHIRNVRVYENGDFIETSHKSSDGTVDIAEV 300 Query: 301 VKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDNVKGAN 358 V+AYHE+G+TGY RPDHGRH+W EE CRPGYGLYDRALGIMYL GVWD L+ ++ N Sbjct: 301 VRAYHESGFTGYARPDHGRHIWDEE--CRPGYGLYDRALGIMYLWGVWDGLERIRKGN 356 >A6M2D7.1 A6M2D7_CLOB8 Mannonate dehydratase EC=4.2.1.8 Length = 353 Score = 500 bits (1288), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/356 (66%), Positives = 284/356 (79%), Gaps = 4/356 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M M +RWYGEGND +TL+ I+QIPGV IVW+LH+ AG+ W ME+I EV GF+ Sbjct: 1 MKMGFRWYGEGNDTVTLEQIKQIPGVETIVWALHDMAAGDEWPMEKILEVKKSADKYGFN 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVH+DIK+GLPTRDKYI+ Y TI KLAKVGVKVICYNFMPVFDW RT+LYK+ Sbjct: 61 LDVVESVNVHEDIKLGLPTRDKYIENYKKTIEKLAKVGVKVICYNFMPVFDWLRTDLYKK 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 DGS ALFYEKA IT+ +P E+V++I TMPGWEPERL L +LF AY +VTEE Sbjct: 121 TEDGSTALFYEKARITNIDPMELVNKIASNP-DLTMPGWEPERLKNLTQLFEAYKNVTEE 179 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 L+ NLKYFLE+IIPV E DIKMA+HPDDP W IFGLPRI++SR+ + R+L VDSPYN Sbjct: 180 DLWNNLKYFLEQIIPVAEINDIKMAVHPDDPPWSIFGLPRIVKSRNDLARLLKLVDSPYN 239 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAE 299 LT C+G++GSNP ND+PAMIREF +RI FAHIRNVK++ENGDFIE SHR DGS+D+ + Sbjct: 240 CLTLCSGAIGSNPNNDVPAMIREFGNRIAFAHIRNVKIYENGDFIETSHRTCDGSLDICD 299 Query: 300 VVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDNVK 355 +VKAYHE G+TGY RPDHGRH+W E+ CRPGYGLYDRALGIMY+ G+WD+L K Sbjct: 300 IVKAYHEFGFTGYARPDHGRHIWNEQ--CRPGYGLYDRALGIMYIWGIWDTLQRTK 353 >A6M0R4.1 A6M0R4_CLOB8 Mannonate dehydratase EC=4.2.1.8 Length = 353 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 239/356 (67%), Positives = 286/356 (80%), Gaps = 4/356 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M M +RWYGEGND +TL+ I+QIPGV GIVW+LH+ AGE W ME+I EV GF+ Sbjct: 1 MKMGFRWYGEGNDTVTLEQIKQIPGVGGIVWALHDMAAGEEWPMEKILEVKKSADKYGFN 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVH+DIK+GLPTRDKYI+ Y TI KLAKVGVKVICYNFMPVFDW RT+L+K+ Sbjct: 61 MDVVESVNVHEDIKLGLPTRDKYIENYKKTIEKLAKVGVKVICYNFMPVFDWLRTDLFKK 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 DGS ALFYEKA I D +P E+V++I TMPGWEPERL L +LF AY +VTEE Sbjct: 121 AEDGSTALFYEKAKIKDIDPMELVNKIASNP-DLTMPGWEPERLKNLSQLFEAYKNVTEE 179 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 L+ NLKYFLE+II V E DIKMA+HPDDP WPIFGLPRI+++RD + R+L VD+PYN Sbjct: 180 DLWNNLKYFLEQIISVAEINDIKMAVHPDDPPWPIFGLPRIVKNRDDLARLLRLVDNPYN 239 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAE 299 LT C+G++GS+P N++PAMIREF +RI FAHIRNVK++ENGDFIE SHR DGS+D+ + Sbjct: 240 CLTLCSGAIGSDPNNNVPAMIREFGNRIAFAHIRNVKIYENGDFIETSHRTCDGSLDICD 299 Query: 300 VVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDNVK 355 +VKAYHE G+TGYVRPDHGRH+W E+ CRPGYGLYDRALGIMYL G+WDSL+ K Sbjct: 300 IVKAYHEVGFTGYVRPDHGRHIWNEQ--CRPGYGLYDRALGIMYLWGIWDSLERNK 353 >Q5WKD5.1 UXUA_BACSK Mannonate dehydratase EC=4.2.1.8 Length = 358 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 229/353 (64%), Positives = 289/353 (81%), Gaps = 4/353 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M MT+RWYGE ND++TL+ I+QIPGV G+VW+LH+KVAGEVW +E I +V +Q GF Sbjct: 1 MRMTFRWYGENNDSVTLEQIKQIPGVEGLVWALHDKVAGEVWPLEDIMQVKEQADRYGFH 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVES+NVH+DIK+GLPTRD YI+ Y ++IR +AKVG KVICYNFMPVFDWTRT+L+KE Sbjct: 61 LDVVESINVHEDIKLGLPTRDAYIENYKESIRNVAKVGAKVICYNFMPVFDWTRTDLFKE 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 + DGS ALFYEKA + + +PRE+V + A FTMPGWEPERLA +++ AY +VTE+ Sbjct: 121 MEDGSTALFYEKAKVDNMDPRELVRQTTSNAA-FTMPGWEPERLAHIEKSLKAYENVTED 179 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 L+E+L+YFLE+++PV EE I+MAIHPDDP W +FGLPRII S + R L +SP + Sbjct: 180 DLWEHLQYFLEQVLPVAEEHGIQMAIHPDDPPWSVFGLPRIITSEAAVERFLQLSNSPAH 239 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAE 299 G+T C+GSLG+NPEND+P +IR FHDRI FAHIRNVK++ENGDFIE SHR +DGSV++A+ Sbjct: 240 GITLCSGSLGANPENDIPKIIRRFHDRIPFAHIRNVKIYENGDFIETSHRSQDGSVNIAD 299 Query: 300 VVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLD 352 VVKAYHENG+TGYVRPDHGRH+W E+ CRPGYGLYDRALGIM+L G+WD+ + Sbjct: 300 VVKAYHENGFTGYVRPDHGRHIWNEK--CRPGYGLYDRALGIMHLWGLWDAYE 350 >B3K847.1 B3K847_9BACI Mannonate dehydratase EC=4.2.1.8 Length = 365 Score = 492 bits (1267), Expect = e-137, Method: Compositional matrix adjust. Identities = 231/359 (64%), Positives = 282/359 (78%), Gaps = 4/359 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M M +RW+GEGND ++LD IRQIPGV GIVW+LH+ GE W MERI+E+ADQ + G Sbjct: 1 MRMVFRWFGEGNDTVSLDQIRQIPGVEGIVWALHDIPVGEEWPMERIEEIADQAKAYGLH 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVH+DIK+GLP+RD+YI YI TI KL +VGVKVICYNFMPVFDW RT+L+K+ Sbjct: 61 IDVVESVNVHEDIKLGLPSRDQYIANYIRTIEKLGQVGVKVICYNFMPVFDWARTDLHKQ 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 PDGS ALF+EKA I +P E+V I + + TMPGWEPERLA L LF AY VTEE Sbjct: 121 TPDGSTALFFEKAKIHGIDPMELVQTINRNSA-LTMPGWEPERLAHLTSLFEAYRGVTEE 179 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 L+ NL+YFL +IP E+ I+MAIHPDDP WPIFGLPR+I S++ +RR L DSP N Sbjct: 180 DLWSNLEYFLSAVIPAAEKNGIRMAIHPDDPPWPIFGLPRVITSQENLRRFLQLYDSPAN 239 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAE 299 LT C+GSLG+NP+ND+ +MI EFHDRI FAHIRNV+V+ENGDFIE SHR DG+VD++ Sbjct: 240 SLTLCSGSLGANPDNDIVSMIHEFHDRIPFAHIRNVRVYENGDFIETSHRTEDGTVDISG 299 Query: 300 VVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDNVKGAN 358 +V+AYH+NG+TGYVRPDHGRH+WGE+ CRPGYGLYDRALGIMYL G+WD+ + + Sbjct: 300 IVQAYHDNGFTGYVRPDHGRHIWGEQ--CRPGYGLYDRALGIMYLWGLWDAYSRERAGS 356 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.141 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,369,110,903 Number of extensions: 104518781 Number of successful extensions: 230503 Number of sequences better than 1.0e-05: 459 Number of HSP's gapped: 229581 Number of HSP's successfully gapped: 649 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)