BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0003.p (177 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2RLC9.1 Q2RLC9_MOOTA Mannitol dehydrogenase 206 7e-52 B8I4H3.1 B8I4H3_CLOCE Mannitol dehydrogenase domain protein 192 8e-48 A6M0R3.1 A6M0R3_CLOB8 Mannitol dehydrogenase, C-terminal domain 185 1e-45 C0C7S3.1 C0C7S3_9FIRM Putative uncharacterized protein 184 3e-45 C0CSY6.1 C0CSY6_9CLOT Putative uncharacterized protein 183 6e-45 >Q2RLC9.1 Q2RLC9_MOOTA Mannitol dehydrogenase Length = 538 Score = 206 bits (524), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 97/165 (58%), Positives = 123/165 (74%), Gaps = 1/165 (0%) Query: 1 MLRLTRSSIQQEQAAWTAAGVELPQFDYEQVAKNTQQKPEWIHFGAGNIFRGFVANAHQK 60 ML L + SI+ ++A W AG+ELP+FDYE++ +T++ P W+HFGAGNIFRGF+A QK Sbjct: 1 MLDLDKDSIKYKKA-WQDAGIELPEFDYEKMVADTKENPTWVHFGAGNIFRGFIAMLQQK 59 Query: 61 LLDSGKAATGIIAAETFDFEMIDKVYKPYDNLTLLVLMNARGDFQKRIVNSIVEGITADK 120 LL+ GKA TGI+A ETFDFE+ID++Y PYDNL+LLV+MN G K++V SI EG+ D Sbjct: 60 LLNLGKAKTGIVAVETFDFEIIDRIYVPYDNLSLLVIMNFDGTLDKKVVASISEGLIGDV 119 Query: 121 SREADDRRLTEVFENPSLQMASFTITEKGYSLTGPNGKYLGIVEK 165 SRE D RL E+F P LQMASFTITEKGYSL +G+Y V K Sbjct: 120 SREHDWNRLKEIFAKPCLQMASFTITEKGYSLKNASGEYFDDVLK 164 >B8I4H3.1 B8I4H3_CLOCE Mannitol dehydrogenase domain protein Length = 537 Score = 192 bits (489), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 86/159 (54%), Positives = 121/159 (76%), Gaps = 1/159 (0%) Query: 2 LRLTRSSIQQEQAAWTAAGVELPQFDYEQVAKNTQQKPEWIHFGAGNIFRGFVANAHQKL 61 ++L R+ I+ + +AW A VE+P+FDY++V T+++P+W+HFG GNIFRGF+A Q L Sbjct: 1 MKLNRNGIK-DYSAWAANQVEMPKFDYDKVLGKTKEEPKWLHFGGGNIFRGFIAALQQNL 59 Query: 62 LDSGKAATGIIAAETFDFEMIDKVYKPYDNLTLLVLMNARGDFQKRIVNSIVEGITADKS 121 L+SGKA +GII AETFD+E+IDKVYKP+DNL+LLV +N G +K+++ S+ E +T D + Sbjct: 60 LNSGKADSGIIVAETFDYEIIDKVYKPFDNLSLLVHLNPDGSLEKKVIASVSESLTGDTA 119 Query: 122 READDRRLTEVFENPSLQMASFTITEKGYSLTGPNGKYL 160 R D RL +F PSLQM SFT+TEKGYSLTG + ++L Sbjct: 120 RTKDWERLKYIFRKPSLQMVSFTVTEKGYSLTGMSNEFL 158 >A6M0R3.1 A6M0R3_CLOB8 Mannitol dehydrogenase, C-terminal domain Length = 537 Score = 185 bits (470), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 81/149 (54%), Positives = 114/149 (76%), Gaps = 1/149 (0%) Query: 4 LTRSSIQQEQAAWTAAGVELPQFDYEQVAKNTQQKPEWIHFGAGNIFRGFVANAHQKLLD 63 L++ SI+ W AG+E+P+FDY++++ +T++ P W+HFGAGNIFRGF+A Q+LL+ Sbjct: 8 LSKESIKNSDL-WEKAGIEIPKFDYDKMSASTKENPTWVHFGAGNIFRGFIAILQQELLN 66 Query: 64 SGKAATGIIAAETFDFEMIDKVYKPYDNLTLLVLMNARGDFQKRIVNSIVEGITADKSRE 123 +GKA +GI+A E +D+E+ID++Y PYDNL+LLV+M G K++V SI E + D SRE Sbjct: 67 TGKAKSGIVAVEGYDYEIIDEIYSPYDNLSLLVIMKPDGSLDKKVVGSIGESLAGDYSRE 126 Query: 124 ADDRRLTEVFENPSLQMASFTITEKGYSL 152 D RL E+F NPSLQ+ASFTITEKGYS+ Sbjct: 127 EDWNRLKEIFSNPSLQIASFTITEKGYSV 155 >C0C7S3.1 C0C7S3_9FIRM Putative uncharacterized protein Length = 537 Score = 184 bits (467), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 82/163 (50%), Positives = 116/163 (71%) Query: 2 LRLTRSSIQQEQAAWTAAGVELPQFDYEQVAKNTQQKPEWIHFGAGNIFRGFVANAHQKL 61 +++ SSI ++A W GV+LPQFD+ + T++ P W+HFGAGNIFRGF+A QKL Sbjct: 1 MQMNASSIVNQKAEWEKLGVKLPQFDHAAMTAATKEHPIWVHFGAGNIFRGFIAALQQKL 60 Query: 62 LDSGKAATGIIAAETFDFEMIDKVYKPYDNLTLLVLMNARGDFQKRIVNSIVEGITADKS 121 L++G+A GIIAA+TFD+++IDK+Y PYDNLT++V +N G + I+ S+ EG+ AD S Sbjct: 61 LNAGEADRGIIAADTFDYDIIDKIYTPYDNLTMMVTLNPDGSTSREIIGSVAEGLRADSS 120 Query: 122 READDRRLTEVFENPSLQMASFTITEKGYSLTGPNGKYLGIVE 164 A R E+F +P LQM SFTITEKGY+L P+G + +V+ Sbjct: 121 DAAMMARFKEIFTDPGLQMISFTITEKGYALYRPDGSLMPVVQ 163 >C0CSY6.1 C0CSY6_9CLOT Putative uncharacterized protein Length = 537 Score = 183 bits (464), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 84/148 (56%), Positives = 105/148 (70%) Query: 16 WTAAGVELPQFDYEQVAKNTQQKPEWIHFGAGNIFRGFVANAHQKLLDSGKAATGIIAAE 75 W AGV+LP+F E + NT++ P W+HFGAGNIFR F+A Q+LL+ GKA GIIA + Sbjct: 14 WENAGVKLPEFSIETMRANTRENPVWVHFGAGNIFRAFIARLQQRLLNEGKADRGIIAVD 73 Query: 76 TFDFEMIDKVYKPYDNLTLLVLMNARGDFQKRIVNSIVEGITADKSREADDRRLTEVFEN 135 TFD++++DK+Y PYDNLT+LVLMNA G K IV SI EG+ D S D RL E+F Sbjct: 74 TFDYDILDKIYAPYDNLTMLVLMNADGRLDKEIVASISEGLKGDSSDGTDYSRLKEIFRK 133 Query: 136 PSLQMASFTITEKGYSLTGPNGKYLGIV 163 PSLQM SFTITEKGY+L +G Y +V Sbjct: 134 PSLQMISFTITEKGYALKNLDGAYFPVV 161 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 970,677,348 Number of extensions: 37809142 Number of successful extensions: 84416 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 84456 Number of HSP's successfully gapped: 56 Length of query: 177 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 50 Effective length of database: 1,864,227,298 Effective search space: 93211364900 Effective search space used: 93211364900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)