BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0001.p (471 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B4BME4.1 B4BME4_9BACI Glucuronate isomerase EC=5.3.1.12 527 e-147 A4IP75.1 UXAC_GEOTN Uronate isomerase EC=5.3.1.12 527 e-147 Q5WJC1.1 UXAC_BACSK Uronate isomerase EC=5.3.1.12 516 e-144 Q7N9X5.1 UXAC_PHOLL Uronate isomerase EC=5.3.1.12 516 e-144 A7B0H1.1 A7B0H1_RUMGN Putative uncharacterized protein 515 e-144 >B4BME4.1 B4BME4_9BACI Glucuronate isomerase EC=5.3.1.12 Length = 467 Score = 527 bits (1357), Expect = e-147, Method: Compositional matrix adjust. Identities = 241/466 (51%), Positives = 321/466 (68%) Query: 1 MKAFIHEDFLLQTEEARVLYHEYAEALPIIDYHCHLDPQAIAENKRFGSITEVWLGGDHY 60 M+ FI + FLLQ + A VLYH+YA++LPIIDYHCHL + IAE++RF +TE+WL GDHY Sbjct: 1 MQPFIDDRFLLQNKHAEVLYHDYAKSLPIIDYHCHLSAKEIAEDRRFHDMTELWLEGDHY 60 Query: 61 KWRALRTFGVEEKYITGNASDEEKFAKWSEVLPYTLGNPLYHWSALELKTYFGVEEQLSP 120 KWRA+R GVEEKYITG+AS EEKF W++ +PY +GNPLYHW+ LELK YF V L+ Sbjct: 61 KWRAMRALGVEEKYITGSASPEEKFQAWAKTVPYCIGNPLYHWTHLELKRYFQVGVLLNE 120 Query: 121 QNWRQIYDHCNTLIAQDGFTAQGLITRSNVKWICTTDDPADDLRYHGQIAGQPGFQTGVT 180 Q W++++DHCN L+ Q+G++A+ + +SNV+WI TTDDP DDL H +IA F V Sbjct: 121 QTWKEVWDHCNELLQQEGYSARSFMIQSNVEWIGTTDDPLDDLMDHQKIAQDSSFSIKVV 180 Query: 181 PTFRPDKAMQIEAESFPDYVRSLEQAAGYAITSYALMERALLERIEYFDKHGCKVSDHGF 240 P+FRPD ++I SF DYV L + A ++ Y + +AL R+ YF + GC+++DHG Sbjct: 181 PSFRPDAVIEINRPSFLDYVSKLGEVADLSVDDYGQLLQALENRVRYFHEAGCRMADHGL 240 Query: 241 SHLPFAEAAPSVLEDIFSRRLSGGTLSRLESDQYQTALLLAMGRKYAAHGWAMQLHIGAI 300 +P+AE IF +R G LSR E ++YQT L + R Y + GW MQLHIG+I Sbjct: 241 ESMPYAECTLDEARVIFQKRKEGFALSREEEEKYQTFTLCFLARLYYSLGWVMQLHIGSI 300 Query: 301 RNPNSRMFRELGPDTGFDTIGDNSYANSLYKLLDGLERTGELPRTILYNLNAVQNDVLAA 360 RN N +MF++LGP+TG+D+I D +A L L+ LER G+LP+TI+Y LN N ++A+ Sbjct: 301 RNTNQKMFQQLGPNTGYDSINDFFFAQPLNAFLNRLERDGQLPKTIVYTLNPAYNYIVAS 360 Query: 361 MAXXXXXXXXXXXXXXXXXWWYNDQKDGMIKQLTSLSNLGLLSCFVGMLTDSRSFLSYTR 420 WW+ND +DG+I+ L L+N+G+ S FVGMLTDSRSFLSY R Sbjct: 361 TIGNFPSEGVKGKVQFGAAWWFNDHQDGIIRHLNDLANVGVFSTFVGMLTDSRSFLSYVR 420 Query: 421 HEYFRRILCNLIGGWVASGDAPGDLAFLGSIVQDICYHNAERYFEF 466 HEYFRRI+CNLIG W+ G+AP D AFLG IVQDICY NA++YF+ Sbjct: 421 HEYFRRIVCNLIGSWIEKGEAPQDYAFLGKIVQDICYFNAKQYFDL 466 >A4IP75.1 UXAC_GEOTN Uronate isomerase EC=5.3.1.12 Length = 467 Score = 527 bits (1357), Expect = e-147, Method: Compositional matrix adjust. Identities = 241/466 (51%), Positives = 321/466 (68%) Query: 1 MKAFIHEDFLLQTEEARVLYHEYAEALPIIDYHCHLDPQAIAENKRFGSITEVWLGGDHY 60 M+ FI + FLLQ + A VLYH+YA++LPIIDYHCHL + IAE++RF +TE+WL GDHY Sbjct: 1 MQPFIDDRFLLQNKHAEVLYHDYAKSLPIIDYHCHLSAKEIAEDRRFHDMTELWLEGDHY 60 Query: 61 KWRALRTFGVEEKYITGNASDEEKFAKWSEVLPYTLGNPLYHWSALELKTYFGVEEQLSP 120 KWRA+R GVEEKYITG+AS EEKF W++ +PY +GNPLYHW+ LELK YF V L+ Sbjct: 61 KWRAMRALGVEEKYITGSASPEEKFQAWAKTVPYCIGNPLYHWTHLELKRYFQVGVLLNE 120 Query: 121 QNWRQIYDHCNTLIAQDGFTAQGLITRSNVKWICTTDDPADDLRYHGQIAGQPGFQTGVT 180 Q W++++DHCN L+ Q+G++A+ + +SNV+WI TTDDP DDL H +IA F V Sbjct: 121 QTWKEVWDHCNELLQQEGYSARSFMIQSNVEWIGTTDDPLDDLMDHQKIAQDSSFSIKVV 180 Query: 181 PTFRPDKAMQIEAESFPDYVRSLEQAAGYAITSYALMERALLERIEYFDKHGCKVSDHGF 240 P+FRPD ++I SF DYV L + A ++ Y + +AL R+ YF + GC+++DHG Sbjct: 181 PSFRPDAVIEINRPSFLDYVSKLGEVADLSVDDYGQLLQALENRVRYFHEAGCRMADHGL 240 Query: 241 SHLPFAEAAPSVLEDIFSRRLSGGTLSRLESDQYQTALLLAMGRKYAAHGWAMQLHIGAI 300 +P+AE IF +R G LSR E ++YQT L + R Y + GW MQLHIG+I Sbjct: 241 ESMPYAECTLDEARVIFQKRKEGFALSREEEEKYQTFTLCFLARLYYSLGWVMQLHIGSI 300 Query: 301 RNPNSRMFRELGPDTGFDTIGDNSYANSLYKLLDGLERTGELPRTILYNLNAVQNDVLAA 360 RN N +MF++LGP+TG+D+I D +A L L+ LER G+LP+TI+Y LN N ++A+ Sbjct: 301 RNTNQKMFQQLGPNTGYDSINDFFFAQPLNAFLNRLERDGQLPKTIVYTLNPAYNYIVAS 360 Query: 361 MAXXXXXXXXXXXXXXXXXWWYNDQKDGMIKQLTSLSNLGLLSCFVGMLTDSRSFLSYTR 420 WW+ND +DG+I+ L L+N+G+ S FVGMLTDSRSFLSY R Sbjct: 361 TIGNFPSEGVKGKVQFGAAWWFNDHQDGIIRHLNDLANVGVFSTFVGMLTDSRSFLSYVR 420 Query: 421 HEYFRRILCNLIGGWVASGDAPGDLAFLGSIVQDICYHNAERYFEF 466 HEYFRRI+CNLIG W+ G+AP D AFLG IVQDICY NA++YF+ Sbjct: 421 HEYFRRIVCNLIGSWIEKGEAPQDYAFLGKIVQDICYFNAKQYFDL 466 >Q5WJC1.1 UXAC_BACSK Uronate isomerase EC=5.3.1.12 Length = 472 Score = 516 bits (1329), Expect = e-144, Method: Compositional matrix adjust. Identities = 246/466 (52%), Positives = 314/466 (67%) Query: 2 KAFIHEDFLLQTEEARVLYHEYAEALPIIDYHCHLDPQAIAENKRFGSITEVWLGGDHYK 61 KAFIHE+F+LQ + A LYH YA+ LPIIDYHCH+ PQ IAEN++F +I+E+WL GDHYK Sbjct: 6 KAFIHENFMLQNKTAETLYHTYAKTLPIIDYHCHVPPQEIAENRQFNNISEIWLHGDHYK 65 Query: 62 WRALRTFGVEEKYITGNASDEEKFAKWSEVLPYTLGNPLYHWSALELKTYFGVEEQLSPQ 121 WRA+R GVEE +ITG+ D+EKF KW+E +PYT+GNPLYHW+ LELK YFG++E LS + Sbjct: 66 WRAMRAVGVEETFITGDGDDKEKFLKWAETVPYTMGNPLYHWTHLELKRYFGIDELLSSE 125 Query: 122 NWRQIYDHCNTLIAQDGFTAQGLITRSNVKWICTTDDPADDLRYHGQIAGQPGFQTGVTP 181 I+ +A + QG+I SNVK ICTTDDP+D L H QI + Q V P Sbjct: 126 TAEAIWSATKEQLAAPERSVQGIIKESNVKVICTTDDPSDHLEAHQQIRKEGACQAAVYP 185 Query: 182 TFRPDKAMQIEAESFPDYVRSLEQAAGYAITSYALMERALLERIEYFDKHGCKVSDHGFS 241 FRPDKA+ A SF Y+ +L AA I+S + AL +R +F + GC +SDHG Sbjct: 186 AFRPDKALVASAPSFVPYLETLGAAAEVDISSLRSLLEALEKRATFFAEEGCVLSDHGLR 245 Query: 242 HLPFAEAAPSVLEDIFSRRLSGGTLSRLESDQYQTALLLAMGRKYAAHGWAMQLHIGAIR 301 LPF + E F + L+ TL+ E ++YQT +LL + R Y GW MQ H+GA+R Sbjct: 246 TLPFVDTTEKEAEAAFQKALNQETLTPQEEEKYQTYVLLFLARLYNKLGWTMQFHLGALR 305 Query: 302 NPNSRMFRELGPDTGFDTIGDNSYANSLYKLLDGLERTGELPRTILYNLNAVQNDVLAAM 361 N NSRM ++GPD+GFD++ D+ +A SL + L+ LERT ELP+TILY LN + N+V+A Sbjct: 306 NNNSRMVEQVGPDSGFDSMADDRFAESLNRFLNELERTNELPKTILYTLNPIFNEVIATT 365 Query: 362 AXXXXXXXXXXXXXXXXXWWYNDQKDGMIKQLTSLSNLGLLSCFVGMLTDSRSFLSYTRH 421 WW+ND KDGM KQ+T L+N GLLS FVGMLTDSRSFLSYTRH Sbjct: 366 IGNFQGGGIPGKIQFGSGWWFNDTKDGMEKQMTDLANNGLLSLFVGMLTDSRSFLSYTRH 425 Query: 422 EYFRRILCNLIGGWVASGDAPGDLAFLGSIVQDICYHNAERYFEFP 467 EYFRRILCN IG WV G+ P D +LG +V+DI Y+NA+RYF FP Sbjct: 426 EYFRRILCNRIGEWVERGEWPADEKWLGKVVEDISYYNAKRYFAFP 471 >Q7N9X5.1 UXAC_PHOLL Uronate isomerase EC=5.3.1.12 Length = 474 Score = 516 bits (1328), Expect = e-144, Method: Compositional matrix adjust. Identities = 249/470 (52%), Positives = 323/470 (68%), Gaps = 3/470 (0%) Query: 1 MKAFIHEDFLLQTEEARVLYHEYAEALPIIDYHCHLDPQAIAENKRFGSITEVWLGGDHY 60 MK F+ +DFLL E AR LYHE+A +PI DYHCHL+P+ IAEN+RF ++ ++WL GDHY Sbjct: 1 MKNFMCDDFLLNNETARQLYHEHAADMPIYDYHCHLNPREIAENRRFDNLGQIWLEGDHY 60 Query: 61 KWRALRTFGVEEKYITGNAS-DEEKFAKWSEVLPYTLGNPLYHWSALELKTYFGVEEQL- 118 KWRA+R G++E ITG + D EK+ W+E +P TLGNPLYHW+ LEL+ FG+ L Sbjct: 61 KWRAMRCAGIDESLITGKQTRDYEKYQAWAETVPLTLGNPLYHWTHLELRRPFGISGILF 120 Query: 119 SPQNWRQIYDHCNTLIAQDGFTAQGLITRSNVKWICTTDDPADDLRYHGQIAGQPGFQTG 178 PQ +I+ CN +A F+A+G++ + NV+ + TTDDP D L+YH QIA F Sbjct: 121 GPQTADEIWHQCNEKLAAPAFSARGIMQQMNVRMVGTTDDPVDSLQYHRQIATDESFDIE 180 Query: 179 VTPTFRPDKAMQIEAESFPDYVRSLEQAAGYAITSYALMERALLERIEYFDKHGCKVSDH 238 V P++RPD+ +IE F DY++ LE A +I ++A + AL R+E+F HGC+ +DH Sbjct: 181 VLPSWRPDRVFKIELAGFIDYIKQLEAVADVSIVAFADVLNALERRLEHFAAHGCRAADH 240 Query: 239 GFSHLPFAEAA-PSVLEDIFSRRLSGGTLSRLESDQYQTALLLAMGRKYAAHGWAMQLHI 297 G +L +A +VL I +RL+GG LS LE QY TA+L+ +GR+YAA GW MQ+HI Sbjct: 241 GIENLRYAPIPDETVLNGILQKRLAGGILSELEIAQYTTAILVWLGRQYAARGWVMQMHI 300 Query: 298 GAIRNPNSRMFRELGPDTGFDTIGDNSYANSLYKLLDGLERTGELPRTILYNLNAVQNDV 357 GAIRN NSRMFR LG D+GFD+IGDN+ A L +LLD ++ T ELP+TILY LN N+V Sbjct: 301 GAIRNNNSRMFRLLGADSGFDSIGDNNIAWPLSRLLDSMDVTDELPKTILYCLNPRDNEV 360 Query: 358 LAAMAXXXXXXXXXXXXXXXXXWWYNDQKDGMIKQLTSLSNLGLLSCFVGMLTDSRSFLS 417 +A MA WW+NDQKDGM++QL LS LGLLS FVGMLTDSRSFLS Sbjct: 361 IATMASNFQGGGIAGKVQFGSGWWFNDQKDGMLRQLEQLSQLGLLSQFVGMLTDSRSFLS 420 Query: 418 YTRHEYFRRILCNLIGGWVASGDAPGDLAFLGSIVQDICYHNAERYFEFP 467 YTRHEYFRRILCN++G W G+ P A LG IV+DIC++NA RYF P Sbjct: 421 YTRHEYFRRILCNVLGQWAEKGEIPNHKAMLGQIVEDICFNNARRYFALP 470 >A7B0H1.1 A7B0H1_RUMGN Putative uncharacterized protein Length = 468 Score = 515 bits (1327), Expect = e-144, Method: Compositional matrix adjust. Identities = 245/468 (52%), Positives = 323/468 (69%) Query: 1 MKAFIHEDFLLQTEEARVLYHEYAEALPIIDYHCHLDPQAIAENKRFGSITEVWLGGDHY 60 MKAF+ +DFLL+TE A++LYHEYA +P++DYHCH+ PQ IAE+++F +IT+VWLGGDHY Sbjct: 1 MKAFMDQDFLLETETAKMLYHEYAAKMPVLDYHCHISPQEIAEDRQFENITQVWLGGDHY 60 Query: 61 KWRALRTFGVEEKYITGNASDEEKFAKWSEVLPYTLGNPLYHWSALELKTYFGVEEQLSP 120 KWR +R+ G+EE+YITG+ASD EKF KW+E L +GNPLYHWS LEL+ YFG L+ Sbjct: 61 KWRQMRSNGIEERYITGDASDREKFQKWAETLERAIGNPLYHWSHLELQRYFGYHGALNA 120 Query: 121 QNWRQIYDHCNTLIAQDGFTAQGLITRSNVKWICTTDDPADDLRYHGQIAGQPGFQTGVT 180 + ++++ CN + +A+ LI +SNV ICTTDDPADDLR+H Q+ F+ V Sbjct: 121 KTAEEVWNLCNEKLQSPQMSARNLIRQSNVTLICTTDDPADDLRWHKQLKEDDSFEVQVL 180 Query: 181 PTFRPDKAMQIEAESFPDYVRSLEQAAGYAITSYALMERALLERIEYFDKHGCKVSDHGF 240 P +RPD AM+IE F Y++ L +AAG I S+A M+ AL +R+E+FD+ GC+ SDHG Sbjct: 181 PAWRPDLAMKIEKLGFAAYLKKLGEAAGMEIHSFADMKDALTKRMEFFDEMGCRASDHGL 240 Query: 241 SHLPFAEAAPSVLEDIFSRRLSGGTLSRLESDQYQTALLLAMGRKYAAHGWAMQLHIGAI 300 + + A+ +E IF + ++G +S+ E +Y+TA +L GR+Y GWAMQLH G Sbjct: 241 GYAMYVPASEEEIEAIFEKGMAGEPISKEEELKYKTAFMLYAGREYHRLGWAMQLHYGCK 300 Query: 301 RNPNSRMFRELGPDTGFDTIGDNSYANSLYKLLDGLERTGELPRTILYNLNAVQNDVLAA 360 R+ + + FR+LGPDTGFD I + + L LL+ L T ELP+TILY+LN N + Sbjct: 301 RDNDVKRFRKLGPDTGFDCIDTYTPSAQLADLLNALNVTDELPKTILYSLNPNDNAAIGT 360 Query: 361 MAXXXXXXXXXXXXXXXXXWWYNDQKDGMIKQLTSLSNLGLLSCFVGMLTDSRSFLSYTR 420 + WW+ND K GMI Q+TSL+NLGLLS FVGMLTDSRSFLSYTR Sbjct: 361 IIGCFQDAGVVGKIQQGSAWWFNDHKQGMIDQMTSLANLGLLSNFVGMLTDSRSFLSYTR 420 Query: 421 HEYFRRILCNLIGGWVASGDAPGDLAFLGSIVQDICYHNAERYFEFPL 468 HEYFRRILCNLIGGWV +G+ P D LG IVQDI Y+NA RYF F + Sbjct: 421 HEYFRRILCNLIGGWVENGEYPADEEILGRIVQDISYNNAVRYFGFEV 468 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,907,587,110 Number of extensions: 122510931 Number of successful extensions: 243894 Number of sequences better than 1.0e-05: 414 Number of HSP's gapped: 242726 Number of HSP's successfully gapped: 414 Length of query: 471 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 332 Effective length of database: 1,751,175,118 Effective search space: 581390139176 Effective search space used: 581390139176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)