BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0004.p (47 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4U2.1 B3K4U2_9BACI Putative uncharacterized protein 87 5e-16 B1D9X0.1 B1D9X0_9BACL Putative uncharacterized protein 79 1e-13 >B3K4U2.1 B3K4U2_9BACI Putative uncharacterized protein Length = 62 Score = 87.0 bits (214), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 41/47 (87%), Positives = 44/47 (93%) Query: 1 MTRIFRPKDPKKFVKEYVRKYRITGGYEEELTMVVEHELVRMNSSVS 47 MTRIFRPKDPKKFVKEYVRKY I GGYEEELT+VVEHEL ++NSSVS Sbjct: 16 MTRIFRPKDPKKFVKEYVRKYHIMGGYEEELTLVVEHELGKLNSSVS 62 >B1D9X0.1 B1D9X0_9BACL Putative uncharacterized protein Length = 62 Score = 79.0 bits (193), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 34/47 (72%), Positives = 45/47 (95%) Query: 1 MTRIFRPKDPKKFVKEYVRKYRITGGYEEELTMVVEHELVRMNSSVS 47 +TRIF+PKDP+KFVK+Y+RKYRITGGYE+ELT++VE E+ ++NSSVS Sbjct: 16 LTRIFQPKDPRKFVKDYIRKYRITGGYEDELTILVEDEMGKINSSVS 62 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 262,533,515 Number of extensions: 6091413 Number of successful extensions: 15508 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 15508 Number of HSP's successfully gapped: 2 Length of query: 47 Length of database: 3,060,696,203 Length adjustment: 20 Effective length of query: 27 Effective length of database: 2,872,275,903 Effective search space: 77551449381 Effective search space used: 77551449381 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)