BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0220.0005.p (326 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DF24.1 B1DF24_9BACL Binding-protein-dependent transport system... 259 3e-67 B3KBW3.1 B3KBW3_9BACI Binding-protein-dependent transport system... 248 8e-64 B3K3M4.1 B3K3M4_9BACI Binding-protein-dependent transport system... 247 9e-64 C4IBC6.1 C4IBC6_CLOBU ABC transporter membrane-spanning permease... 243 1e-62 B1D7R4.1 B1D7R4_9BACL Binding-protein-dependent transport system... 238 8e-61 >B1DF24.1 B1DF24_9BACL Binding-protein-dependent transport systems inner membrane component Length = 307 Score = 259 bits (661), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 132/301 (43%), Positives = 188/301 (62%), Gaps = 1/301 (0%) Query: 27 KSLKKDSTLYLLALPGIVILILFAYLPMSGLILVFKNYNFNDGIFGSPWAGXXXXXXXXX 86 + L K+ TL L+ LP V+L LF YLP++GL++ FK+++F GIFGS W Sbjct: 7 RELFKNRTLLLMFLPVAVLLFLFNYLPLAGLVIAFKDFDFTKGIFGSDWMDPLFDNFDYL 66 Query: 87 XMEDS-LRATRNTVMLNALYMLTGTFFSVAIAIILNELRSKWFIKITQSVMFFPYFISWI 145 D+ RA RNT++LNAL++ G F V A++LNE+R+K+F +++QS+ F P+FISWI Sbjct: 67 FSSDTAFRAVRNTILLNALFITVGLIFEVGFALLLNEVRNKYFKRVSQSLTFLPFFISWI 126 Query: 146 IIGAILFSLLDYDKGIMNQLLQTLGISQVDWYSSPWLFVIILVLANIWKSAGYGAIIYYA 205 ++G ++LLD++ G +N L T+G+ +D+YS L+ IIL +A WK+ GYG IIY A Sbjct: 127 VVGVFAYNLLDFESGAVNHFLTTIGLQPIDFYSEAGLWPIILTIAVRWKATGYGTIIYLA 186 Query: 206 VLQGIDTSYYEAAKIDGASRWQIITRITVPMLIPSXXXXXXXXXXXXXXXDLSMIMGVTF 265 L +D SYYEAA IDGA+RWQ I I++PML P+ D M + Sbjct: 187 ALTSVDNSYYEAASIDGATRWQQIRYISLPMLRPTIIILTLLAVGRIMNADFGMFYAMVG 246 Query: 266 LNPLLLPTTDIIDVYVYRTAIRSGEFGFASAITLYQSVFGFLLVLVANKLAGWYDKDSKL 325 LL PTTD+ID +VYR+ +SG+ G ASA QS+ F+LV+ +N A DKDS + Sbjct: 247 DASLLFPTTDVIDTFVYRSLRKSGDIGMASAAGFLQSMIAFVLVIASNYAARKIDKDSAI 306 Query: 326 F 326 F Sbjct: 307 F 307 >B3KBW3.1 B3KBW3_9BACI Binding-protein-dependent transport systems inner membrane component Length = 330 Score = 248 bits (632), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 130/314 (41%), Positives = 190/314 (60%), Gaps = 6/314 (1%) Query: 18 RTARAKSFR-----KSLKKDSTLYLLALPGIVILILFAYLPMSGLILVFKNYNFNDGIFG 72 R AR + + + L K+ LY +A+PGI+ L++F+Y+P+SG +L FK++ + GI+G Sbjct: 18 RPARMEGYYNLRMFRDLVKNRGLYTMAIPGIIFLVVFSYIPLSGHLLAFKDFQISKGIWG 77 Query: 73 SPWAGXXXXXXXXXXMEDSLRATRNTVMLNALYMLTGTFFSVAIAIILNELRSKWFIKIT 132 S W G D ++ T NTV LNAL+++ F++ IA+ LNE+R KWF + Sbjct: 78 SDWVGLDNFRFFFSN-RDWIQVTVNTVYLNALFIIFEMVFALLIALFLNEIRLKWFKRTI 136 Query: 133 QSVMFFPYFISWIIIGAILFSLLDYDKGIMNQLLQTLGISQVDWYSSPWLFVIILVLANI 192 QSV+F PYFISW+++G + F L + G++N++L G+ VDWY +PW++ IIL L I Sbjct: 137 QSVVFLPYFISWVVVGYMTFILFNNTDGLINRVLSMSGLQSVDWYQTPWVWPIILTLTRI 196 Query: 193 WKSAGYGAIIYYAVLQGIDTSYYEAAKIDGASRWQIITRITVPMLIPSXXXXXXXXXXXX 252 WKSAGY +II A + G + YYE+A++DGA+R Q + IT+P+L P Sbjct: 197 WKSAGYYSIILLAAITGFSSDYYESARLDGATRLQQMKYITLPLLKPQIIILVLLGVGRI 256 Query: 253 XXXDLSMIMGVTFLNPLLLPTTDIIDVYVYRTAIRSGEFGFASAITLYQSVFGFLLVLVA 312 D MI G+ N +L PTTD+ID Y YR + G F +SAI YQS+ G + V+ Sbjct: 257 FYGDFGMIYGIIGDNGILFPTTDVIDTYSYRALRQLGNFSMSSAIVFYQSIMGLIAVVSF 316 Query: 313 NKLAGWYDKDSKLF 326 N L DKDS+LF Sbjct: 317 NALVRKIDKDSRLF 330 >B3K3M4.1 B3K3M4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 311 Score = 247 bits (631), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 129/309 (41%), Positives = 190/309 (61%), Gaps = 1/309 (0%) Query: 18 RTARAKSFRKSLKKDSTLYLLALPGIVILILFAYLPMSGLILVFKNYNFNDGIFGSPWAG 77 R SF + L ++ LYLLALPGI L +FAY+PM+G +L F+NY +DGI+GS W G Sbjct: 4 RRRFGGSFVRELSRNRYLYLLALPGIAFLAVFAYMPMAGHLLAFQNYRLSDGIWGSEWVG 63 Query: 78 XXXXXXXXXXMEDSLRATRNTVMLNALYMLTGTFFSVAIAIILNELRSKWFIKITQSVMF 137 +D LR T NTV LNAL+++TG +V +AI LNE+R++ F +ITQS +F Sbjct: 64 FKNFEFFFNG-KDWLRVTLNTVFLNALFLVTGLGSAVVLAIFLNEIRNRLFKRITQSFIF 122 Query: 138 FPYFISWIIIGAILFSLLDYDKGIMNQLLQTLGISQVDWYSSPWLFVIILVLANIWKSAG 197 PYFISW+++ + +L +G++N+ L LG VDWY +P ++ IL + + WK AG Sbjct: 123 LPYFISWLVVSMMTLTLFSTSEGLINRALAALGYEGVDWYLTPGVWPYILTVISAWKIAG 182 Query: 198 YGAIIYYAVLQGIDTSYYEAAKIDGASRWQIITRITVPMLIPSXXXXXXXXXXXXXXXDL 257 Y +II+ AV+ GI + YYE+A+I+GASR Q + IT+P++ P+ D Sbjct: 183 YNSIIFLAVITGISSEYYESARIEGASRMQQMLYITLPLMRPTVMILALLGIGRIFYGDF 242 Query: 258 SMIMGVTFLNPLLLPTTDIIDVYVYRTAIRSGEFGFASAITLYQSVFGFLLVLVANKLAG 317 MI + N +L PTTD+ID Y +R + G F ++A+ +YQS G + +L+ N +A Sbjct: 243 GMIYAIIGDNGILFPTTDVIDTYAFRALRQLGNFSMSAAVVVYQSSMGLVTILIFNAIAR 302 Query: 318 WYDKDSKLF 326 D DS+LF Sbjct: 303 KVDADSRLF 311 >C4IBC6.1 C4IBC6_CLOBU ABC transporter membrane-spanning permease-sugar transport Length = 326 Score = 243 bits (621), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 118/312 (37%), Positives = 190/312 (60%), Gaps = 1/312 (0%) Query: 15 PGIRTARAKSFRKSLKKDSTLYLLALPGIVILILFAYLPMSGLILVFKNYNFNDGIFGSP 74 P + R + F +++++ L L+ LP I+ ++F+Y+PM G IL FKNYNF DGIFGSP Sbjct: 16 PVDKKNRLRKFMLTIRRNKVLILMLLPAILYFLIFSYIPMGGTILAFKNYNFRDGIFGSP 75 Query: 75 WAGXXXXXXXXXXMEDSLRATRNTVMLNALYMLTGTFFSVAIAIILNELRSKWFIKITQS 134 W G + + TRNT++ N ++ T + +AI+++E+ K+F K+TQ+ Sbjct: 76 WVGLDNFKYFFISGQAWI-VTRNTILYNVAFITVNTILQMGLAILISEIGGKYFKKLTQT 134 Query: 135 VMFFPYFISWIIIGAILFSLLDYDKGIMNQLLQTLGISQVDWYSSPWLFVIILVLANIWK 194 MF PYFISW+++G I +++L+ + G +N ++ +G + ++Y S +++ I++ + WK Sbjct: 135 AMFLPYFISWVVVGIIAYNMLNLENGTINSMIAKIGGEKFNFYGSTFVWPFIMIFVSAWK 194 Query: 195 SAGYGAIIYYAVLQGIDTSYYEAAKIDGASRWQIITRITVPMLIPSXXXXXXXXXXXXXX 254 + GYG ++Y A + GIDT YEAAKIDGA+ +Q I ++T+P LIP+ Sbjct: 195 NVGYGTVLYLAAIMGIDTETYEAAKIDGANIFQRIFKVTIPSLIPTMIILTLLAIGNIFR 254 Query: 255 XDLSMIMGVTFLNPLLLPTTDIIDVYVYRTAIRSGEFGFASAITLYQSVFGFLLVLVANK 314 D M + N L TD+ID + +R+ I+SGE G ++A LYQSVF F+ ++++N Sbjct: 255 GDFQMFYQIIGNNGPLFNATDVIDTFTFRSLIQSGEVGMSAASCLYQSVFCFITIMISNF 314 Query: 315 LAGWYDKDSKLF 326 L YDKD LF Sbjct: 315 LVKKYDKDYSLF 326 >B1D7R4.1 B1D7R4_9BACL Binding-protein-dependent transport systems inner membrane component Length = 325 Score = 238 bits (606), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 128/325 (39%), Positives = 191/325 (58%), Gaps = 8/325 (2%) Query: 2 MQEATPETQGRLSPGIRTARAKSFRKSLKKDSTLYLLALPGIVILILFAYLPMSGLILVF 61 + +TQ R+ P KSF ++++ LYLL LPGI+++ +FAYLPM G+++ F Sbjct: 9 LNNRAHDTQARVKP-------KSFLMEMRRNGLLYLLMLPGILLVFVFAYLPMGGIVVAF 61 Query: 62 KNYNFNDGIFGSPWAGXXXXXXXXXXMEDSLRATRNTVMLNALYMLTGTFFSVAIAIILN 121 +N+N GI GS + G E L NT+ LNAL++L G SV+IA++ + Sbjct: 62 QNFNAIKGITGSEFVGLKNFEFFVQSKE-VLHVIFNTLFLNALFILFGALASVSIAVMFS 120 Query: 122 ELRSKWFIKITQSVMFFPYFISWIIIGAILFSLLDYDKGIMNQLLQTLGISQVDWYSSPW 181 EL KWF +I Q+++ P F+SW +I + +LL D G++N+ L I + +++ P Sbjct: 121 ELGGKWFKRIVQTIVTLPNFLSWTVIAMFVTALLSSDTGMLNKWLAHFHIGPIAFFNEPA 180 Query: 182 LFVIILVLANIWKSAGYGAIIYYAVLQGIDTSYYEAAKIDGASRWQIITRITVPMLIPSX 241 + ILVL IW+ AG+GAI+Y A + GI+ YE+A IDGASRW+ I IT+P+L P+ Sbjct: 181 AWPFILVLIKIWQGAGFGAIVYLATITGINPEIYESASIDGASRWKKIWYITLPLLKPTI 240 Query: 242 XXXXXXXXXXXXXXDLSMIMGVTFLNPLLLPTTDIIDVYVYRTAIRSGEFGFASAITLYQ 301 D MI + N LL PTTD+ID YVYR + G+ G ++A+ ++Q Sbjct: 241 ILMMLLAIGGIFYGDFGMIYALVGRNVLLYPTTDVIDTYVYRALMDLGDMGMSAAVGVFQ 300 Query: 302 SVFGFLLVLVANKLAGWYDKDSKLF 326 SV GF+LV+ N+LA + DS LF Sbjct: 301 SVIGFILVVTVNQLARKFSPDSALF 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,684,879,622 Number of extensions: 61690690 Number of successful extensions: 229910 Number of sequences better than 1.0e-05: 5004 Number of HSP's gapped: 226591 Number of HSP's successfully gapped: 5005 Length of query: 326 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 190 Effective length of database: 1,779,438,163 Effective search space: 338093250970 Effective search space used: 338093250970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)