BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0220.0004.p (319 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DF25.1 B1DF25_9BACL Binding-protein-dependent transport system... 194 7e-48 B3K760.1 B3K760_9BACI Binding-protein-dependent transport system... 192 5e-47 B9MPG1.1 B9MPG1_ANATD Binding-protein-dependent transport system... 191 8e-47 A4XN07.1 A4XN07_CALS8 Binding-protein-dependent transport system... 189 3e-46 A4W3Y8.1 A4W3Y8_STRS2 ABC transporter membrane spanning permease... 187 2e-45 >B1DF25.1 B1DF25_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 194 bits (494), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 103/233 (44%), Positives = 156/233 (66%), Gaps = 3/233 (1%) Query: 16 CIAIFSAICLVPFILAVSGSLSTESRIAAEGYSFWPRGFTFETYEFLF-GSKAQQILRAY 74 I++FS CL PFI+ +SGSLSTE+ I GY+ WP+ TF++Y LF GS +I+ AY Sbjct: 16 VISLFSLSCLFPFIMVISGSLSTENDIINYGYTIWPKHITFDSYRILFLGS--NRIIDAY 73 Query: 75 SMSVIVTVLGTVSAVLVTTAYAYVISVKSFRLKNXXXXXXXXTMLFSGGTLPWYLLSTKY 134 +S++VTV GT+ +V VT+ AYV++ +SF+ +N TMLF+GG +PWY++ Y Sbjct: 74 GISILVTVAGTILSVFVTSMGAYVMARRSFKYRNILSVYVIITMLFNGGLVPWYIICVHY 133 Query: 135 YHLGDTLIGLFIPYVLSVFLMFLMANYFRSIPHEIVESAQIDGAGHLRIFFSIMIPLGRV 194 +L D+L L +P + + F MFL+ N+ SIP EI ESA++DGAG +IF+ +++PL Sbjct: 134 LNLKDSLWALILPMLANAFNMFLIRNFMNSIPEEINESAKMDGAGEFKIFYRLIMPLSLP 193 Query: 195 GLVTISLFYALQFWNDFYLPLMLVTDQKLYTIQYLMYNMMANIQFLASGNAAQ 247 L T+ LF AL +WND++L LM V Q+L +Q L+ +++N++FL S + A Sbjct: 194 VLATVGLFVALSYWNDWFLGLMFVDKQELQPLQLLLRTLVSNVEFLKSSSNAS 246 >B3K760.1 B3K760_9BACI Binding-protein-dependent transport systems inner membrane component Length = 298 Score = 192 bits (487), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 95/289 (32%), Positives = 170/289 (58%), Gaps = 14/289 (4%) Query: 6 NKGLLIFFYCCIAIFSAICLVPFILAVSGSLSTESRIAAEGYSFWPRGFTFETYEFLFGS 65 +K ++ F Y I +F+ +CL P +L +S S S+E +A GYS P + +TY+++F + Sbjct: 10 DKLVIAFAYAFIGLFALVCLYPLVLTLSVSFSSEQAVARNGYSIIPLSPSLDTYKYIFVN 69 Query: 66 KAQQILRAYSMSVIVTVLGTVSAVLVTTAYAYVISVKSFRLKNXXXXXXXXTMLFSGGTL 125 ++L++Y +++ VT +GT A+L+T+ A+ +S+K + +N T++FS G + Sbjct: 70 SGMKLLKSYGVTLFVTTVGTFGALLITSMIAFSLSIKKLKYRNVLAFISNFTIIFSAGLI 129 Query: 126 PWYLLSTKYYHLGDTLIGLFIPYVLSVFLMFLMANYFRSIPHEIVESAQIDGAGHLRIFF 185 PWY++S YY L ++++ L +P + SV+ MFLM YF SI + E+A++DGA + I+ Sbjct: 130 PWYMVSVNYYGLKNSILALILPSIFSVWNMFLMRTYFASISPSLYEAAEMDGANYFTIYV 189 Query: 186 SIMIPLGRVGLVTISLFYALQFWNDFYLPLMLVTDQKLYTIQYLMYNMMANIQFLASGNA 245 I +PL + L+T+ L YALQ+WND++ L+ + ++ L+ +QY +YN+++N+ ++SG Sbjct: 190 KIALPLSKTALLTVGLMYALQYWNDWWHALIFINERDLFPLQYFLYNILSNVNAISSG-- 247 Query: 246 AQVGGXXXXRTAAGDSQNGDDLPDRAANRHI--ISFSPAFFCQRHYRGF 292 R +G S N LP I I+ P F + + + Sbjct: 248 ---------RIPSGASGN-ITLPAETVKMAITVITIGPIIFLYPYIQKY 286 >B9MPG1.1 B9MPG1_ANATD Binding-protein-dependent transport systems inner membrane component Length = 301 Score = 191 bits (485), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 99/257 (38%), Positives = 155/257 (60%), Gaps = 12/257 (4%) Query: 18 AIFSAICLVPFILAVSGSLSTESRIAAEGYSFWPRGFTFETYEFLFGSKAQQILRAYSMS 77 I + CL+P +S SLS +++I GY FWP GF+ ++Y+F+ S+ + I AY ++ Sbjct: 23 GIVTLACLIPLWSVISISLSNDNKIRQIGYRFWPVGFSTKSYDFVL-SQGRAIWHAYGIT 81 Query: 78 VIVTVLGTVSAVLVTTAYAYVISVKSFRLKNXXXXXXXXTMLFSGGTLPWYLLSTKYYHL 137 ++VT++GTV VLV + YAY++ K F+ + TML + G +PWY++ HL Sbjct: 82 ILVTIVGTVLCVLVVSMYAYILFRKDFKYRKFFTFFGFFTMLINAGLVPWYIVCVNILHL 141 Query: 138 GDTLIGLFIPYVLSVFLMFLMANYFR-SIPHEIVESAQIDGAGHLRIFFSIMIPLGRVGL 196 +T+ L +PYV++++ + + Y S+P I+ESA+IDGAG FF I+IPL + GL Sbjct: 142 KNTIYALILPYVMNMWYVLIFRTYLSMSLPDSIIESAKIDGAGEFTTFFRIVIPLVKPGL 201 Query: 197 VTISLFYALQFWNDFYLPLMLVTDQKLYTIQYLMYNMMANIQFLASGNAAQVGGXXXXRT 256 TI+LF A+ +WND++LP ML+ +KLY +QY+MY + IQ+LA A ++G T Sbjct: 202 ATIALFSAITYWNDWWLPFMLIDTEKLYNLQYMMYRVQQKIQYLAE-IAGKLGASGIVPT 260 Query: 257 AAGDSQNGDDLPDRAAN 273 DLP +A Sbjct: 261 ---------DLPTESAR 268 >A4XN07.1 A4XN07_CALS8 Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 189 bits (480), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 92/229 (40%), Positives = 153/229 (66%), Gaps = 2/229 (0%) Query: 14 YCCIAIFSAICLVPFILAVSGSLSTESRIAAEGYSFWPRGFTFETYEFLFGSKAQQILRA 73 Y + +FS CL+PFIL + S+ E+ +AA G++ P+ +F+ Y+ +F K +L A Sbjct: 16 YVFMGLFSIFCLIPFILVIINSIVPEADLAANGFTLIPKRISFDAYKIVF--KGNVVLNA 73 Query: 74 YSMSVIVTVLGTVSAVLVTTAYAYVISVKSFRLKNXXXXXXXXTMLFSGGTLPWYLLSTK 133 Y +++ VTV+GTV +++VT+ AY +SVK+ + +N TMLFSGG +P Y+L T+ Sbjct: 74 YKVTIFVTVVGTVLSMIVTSLMAYPLSVKNLKYRNYIAMYVYITMLFSGGLVPSYILITR 133 Query: 134 YYHLGDTLIGLFIPYVLSVFLMFLMANYFRSIPHEIVESAQIDGAGHLRIFFSIMIPLGR 193 Y HL +T+ IP +++ + +F++ N+F +IP + ESA+IDGA + I + I++PL + Sbjct: 134 YLHLDNTIWVYIIPSLINPWNLFVLRNFFGTIPDSLAESARIDGASEVYILWKIILPLSK 193 Query: 194 VGLVTISLFYALQFWNDFYLPLMLVTDQKLYTIQYLMYNMMANIQFLAS 242 + T+SLFYAL +WN+++ ++ +TDQKLYT+QYL+ +M +IQ L Sbjct: 194 PAIATLSLFYALGYWNEWFKCVLYITDQKLYTLQYLIMAIMRDIQMLTQ 242 >A4W3Y8.1 A4W3Y8_STRS2 ABC transporter membrane spanning permease-sugar transport Length = 307 Score = 187 bits (474), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 100/239 (41%), Positives = 146/239 (61%), Gaps = 2/239 (0%) Query: 6 NKGLLIFFYCCIAIFSAICLVPFILAVSGSLSTESRIAAEGYSFWPRGFTFETYEFLFGS 65 +K +FF + IF+ C +PFI + S++ E+ +A GYSFWP ++ YE+LF Sbjct: 16 SKKTDLFFSSLLGIFALSCTLPFIFVIIISMTDETSLAINGYSFWPEQWSLAGYEYLFQL 75 Query: 66 KAQQILRAYSMSVIVTVLGTVSAVLVTTAYAYVISVKSFRLKNXXXXXXXXTMLFSGGTL 125 K QIL+A ++ VT++GT V T YAY IS SFR + TMLFS GT+ Sbjct: 76 K-DQILQALFITFFVTIVGTSLNVFFTVLYAYAISRNSFRYRRFFTLMALFTMLFSAGTI 134 Query: 126 PWYLLSTKYYHLGDTLIGLFIPYVLSVFLMFLMANYFR-SIPHEIVESAQIDGAGHLRIF 184 P Y++ T + +L DT+ L +P +LS F + +M ++FR +I I+ESA+IDGA RIF Sbjct: 135 PTYIVMTSFLNLKDTVAALILPMLLSPFNIMVMRSFFRKTISESIIESARIDGASEFRIF 194 Query: 185 FSIMIPLGRVGLVTISLFYALQFWNDFYLPLMLVTDQKLYTIQYLMYNMMANIQFLASG 243 F I +PL G+ TI+LF AL FWND+Y L+ + L+ +QYL+ + ANIQ+L+ Sbjct: 195 FQICLPLTLPGIATIALFTALAFWNDWYNALLYIQSDNLFPLQYLLMKIQANIQYLSEN 253 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.142 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,700,909,853 Number of extensions: 63490902 Number of successful extensions: 201405 Number of sequences better than 1.0e-05: 4869 Number of HSP's gapped: 198968 Number of HSP's successfully gapped: 4869 Length of query: 319 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 184 Effective length of database: 1,788,859,178 Effective search space: 329150088752 Effective search space used: 329150088752 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)