BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0220.0003.p (510 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D6I7.1 B1D6I7_9BACL Extracellular solute-binding protein family 1 290 2e-76 B3K759.1 B3K759_9BACI Extracellular solute-binding protein family 1 229 6e-58 B1D8W3.1 B1D8W3_9BACL Extracellular solute-binding protein family 1 150 3e-34 B3KBX6.1 B3KBX6_9BACI Extracellular solute-binding protein family 1 149 8e-34 B1DD38.1 B1DD38_9BACL Extracellular solute-binding protein family 1 135 7e-30 >B1D6I7.1 B1D6I7_9BACL Extracellular solute-binding protein family 1 Length = 514 Score = 290 bits (742), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 174/498 (34%), Positives = 276/498 (55%), Gaps = 9/498 (1%) Query: 11 LLAAGFLVGGCSQGNNESAAPSSGSGNEGTPSVTDNGSKDGSPLAPAKLKIVLYGDESNR 70 +L+ + SQ N ++ S G N GT TDN KD +P + LK+++ GD+S+R Sbjct: 19 ILSLSLVFALFSQYINLGSSKSPGPHNPGT---TDNAGKD-APESFVTLKVIMPGDQSSR 74 Query: 71 MKELAKTEFYDVIKKEINAEVEFHFLPWTEYGGGKTDLMLSTGEDFATY-TDANYMVKSV 129 M + + E D +KK++N +E ++PW+ Y K +L LSTGED+ + A ++ Sbjct: 75 MGDFVENELNDRLKKDLNMNLELTYIPWSNYQQ-KLELALSTGEDYDLFWYGAPFVSVYY 133 Query: 130 AKGLYADLTPYVSA-AADLSKNVDEASFKAFQLDGKQYAIPVGNKPNSAEFYSALVRQDL 188 +KG L + DL K++ E +FK ++DGK +AIP ++ +F S +VRQDL Sbjct: 134 SKGYIQPLDELLGQYGQDLIKHIPEDNFKQNRIDGKLWAIPSQAFTSAGKFSSVMVRQDL 193 Query: 189 LEEAGMTQVTSLKELEQFYDKVHAIHPELIGYANTD-ARRILQYDYTDKNLYWQNDFIAL 247 LE+ GMT + ++ +LEQFY ++HAI GY TD +L + TD+N ++ + Sbjct: 194 LEKVGMTDIKTIADLEQFYQRMHAIDSSYYGYLETDRGHELLWRELTDQNYTSLDERMLF 253 Query: 248 DESAKDDKIISWFETEEFKKYANLMRGWYEKGIIPKYAATNVPQLQSDWNSGKAMFWAGT 307 +++S+ E++ +KK A L W KGII N N+GK +F G Sbjct: 254 MVDEDSGELLSYVESDLYKKVARLRESWVNKGIIDMNLVGNPAAKIDQENAGKLLFRVGA 313 Query: 308 AARPFEGAATISQAVPEAKLVNYFLGSGRPKISRGTYSTAFFVSTAAKDPERYVMFFNLL 367 +R E T A PEA+L Y+L +PK + AF +++A+K+PER +MF N + Sbjct: 314 VSRAMENLQTARNADPEARLREYYLAKEKPKYITTPSNEAFMITSASKNPERAMMFMNWI 373 Query: 368 QKNQELYDLFAYGIKGTDYTVDENGRLTKINTDSLIPDWLLMNKNFIRFDNTVPDEFIAE 427 +++E Y+ YG++G DY + ENG++ ++ D L+ +W+ NK++ V D+ I + Sbjct: 374 LQSKENYNFIIYGVEGKDYVL-ENGKIKQLTNDQLMYEWMWRNKDYFIAPANVEDDVIED 432 Query: 428 YKAWDDGAIISKGAGFNFDNTPVKNEETKLNGVFTEYLAPIGSGFSDYDTAFPKALDRLK 487 A D+ + ISK +GF+F+ PVKNE ++ V+ E PI G + YD + AL LK Sbjct: 433 MLANDENSRISKLSGFHFNEGPVKNEIAQVMAVYKEKFEPINFGIASYDQYYDDALAALK 492 Query: 488 AAGIDKYIAEYQKQFSEW 505 AG D AE +KQFSE+ Sbjct: 493 KAGFDIVFAELKKQFSEF 510 >B3K759.1 B3K759_9BACI Extracellular solute-binding protein family 1 Length = 485 Score = 229 bits (583), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 160/515 (31%), Positives = 251/515 (48%), Gaps = 40/515 (7%) Query: 1 MKKKKGW--LSLLLAAGFLVGGCSQGNNESAAPSSGSGNEGTPSVTDNGSKDGSPLAPAK 58 MK KK L LL+A+ ++ GCS G E AA A Sbjct: 1 MKAKKMLILLVLLIASVSVMAGCS-GKKEGAA------------------------ALET 35 Query: 59 LKIVLYGDESNRMKELAKTEFYDVIKKEINAEVEFHFLPWTEYGGGKTDLMLSTGEDFAT 118 + I+ G+ S+RM E EF + + ++ +VE ++PW +Y K D+ML+ E Sbjct: 36 VTILYPGERSDRMNEFLNNEFAEKMAADLGLKVEVIYVPWAQYWEQK-DIMLAANEPIDL 94 Query: 119 Y----TDANYMVKSVAKGLYADLTPYVSAAADLSKNVDEASFKAFQLDGKQYAIPVGNKP 174 Y D + +V + DL D+ K + + +DGK + IP P Sbjct: 95 YWDGLPDLSTIVNKKQAMVLDDLIQ--EYGQDMLKVLPMEQLQGATIDGKIHGIPSAYAP 152 Query: 175 NSAEFYSALVRQDLLEEAGMTQVTSLKELEQFYDKVHAIHPELIGYANTDARRILQYDYT 234 +SA + VRQD+LE GMT V + +L++F K PE+ G A+ + + +Y +T Sbjct: 153 SSAMYQLVAVRQDILEAVGMTDVKTPDDLKEFATKAKENFPEMKGPADIIFKPLTRY-FT 211 Query: 235 DKNLYW--QNDFIALDESAKDDKIISWFETEEFKKYANLMRGWYEKGIIPKYAATNVPQL 292 D+ W D + E +K K S++ETE F++ A RG YE G+ + + Sbjct: 212 DEQYNWIAVEDLVVFGEDSK--KAYSYYETEAFQEVAKFNRGMYEAGLYTEDLTIKYNER 269 Query: 293 QSDWNSGKAMFWAGTAARPFEGAATISQAVPEAKLVNYFLGSGRPKISRGTYSTAFFVST 352 S +G ++ G+ + E I P+A++ Y L +P+ T + Sbjct: 270 DSRMQTGLYLWVEGSLGKEMEIVDAIKANAPDAEVKTYLLAEDKPRYVTATGGEVLGIPV 329 Query: 353 AAKDPERYVMFFNLLQKNQELYDLFAYGIKGTDYTVDENGRLTKINTDSLIPDWLLMNKN 412 A +PE + F N + K+++ Y YG++G DY + +GR+ K+ +W+ N+N Sbjct: 330 NAPNPEGAMKFVNWIYKSKDNYQFALYGVEGKDYEI-VDGRINKLTPSEFFYEWMFRNQN 388 Query: 413 FIRFDNTVPDEFIAEYKAWDDGAIISKGAGFNFDNTPVKNEETKLNGVFTEYLAPIGSGF 472 + F V E I +YK+WDD A+ S+ GF F+N VK ET L V + LA I SGF Sbjct: 389 YQLFGPNVAQESIDQYKSWDDQAVRSESLGFRFNNEKVKLIETSLKEVAGKDLAAIRSGF 448 Query: 473 SDYDTAFPKALDRLKAAGIDKYIAEYQKQFSEWYA 507 D++T +PKA+ +LKAAGID+Y+AE Q+Q E+ A Sbjct: 449 VDFETEYPKAIQKLKAAGIDEYVAEIQRQLDEFLA 483 >B1D8W3.1 B1D8W3_9BACL Extracellular solute-binding protein family 1 Length = 521 Score = 150 bits (378), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 144/505 (28%), Positives = 237/505 (46%), Gaps = 57/505 (11%) Query: 33 SGSGNEGTPSVTDNGSKDGS-PLAPAKLKIVLYGDESNRMKELAKTEFYDVIKKEINAEV 91 SGSG E + S DN KDGS L P KL +V + +L E + K++INA V Sbjct: 41 SGSGKETSSS--DN--KDGSQTLKPVKLVMVFPAAGEPKDMQLVSDEINKITKEKINATV 96 Query: 92 EFHFLPWTEYGGGKTDLMLSTGED-------FATYTDANYMVKSVAKGLYADLTPYVSAA 144 + + W + +T LM+S E TY+ + VAKG + DL Y+ Sbjct: 97 KLVPIGWGAWTQQRT-LMMSGKEQVDLILSGLGTYS------QDVAKGQFLDLNDYLENE 149 Query: 145 ADLSK----NVDEASFKAFQLDGKQYAIPVGNKPNSAEFYSALVRQDLLEEAGM--TQVT 198 + K N+D A ++DGK YA+P + + A + +R+DL+++ + T + Sbjct: 150 GNGIKEALDNLDPGFLNATKIDGKIYAVP--SIRDLAADFGITMRKDLVDKYNIDTTSIK 207 Query: 199 SLKELEQFYDKVHAIHPELIGYANTDARRILQYDYTDKNLYWQNDFIALDE-------SA 251 SL +L+ + + P+++ ++Y + ++Y ++ F L + + Sbjct: 208 SLDDLDAIFKTIKDNEPDIV--------PTVKYGNSIIDIYVRSAFDTLSDGFGVLPGND 259 Query: 252 KDDKIISWFETEEFKKYANLMRGWYEKGIIPKYAATNVPQLQSDWNSGKAMFWAGTAARP 311 + K++ +E E+K+ + +R WY G I K AAT+ + +GKA W + +P Sbjct: 260 NNLKLVDMYEAPEYKEMLDTVRRWYLAGYIAKDAATSTETQYNMVKAGKAFSWI-SHMKP 318 Query: 312 FEGAATISQAVPEAKLVNYFLGSGRPKISRGTYSTAFFVSTA--AKDPERYVMFFNLLQK 369 G T ++V+ +P IS + T+ S A + DP R +M NL+ Sbjct: 319 --GIETQESRNTAREMVSVHF---KPAISTTSDITSIMWSVARNSADPSRAMMLLNLMYT 373 Query: 370 NQELYDLFAYGIKGTDYT------VDENGRLTKINTD-SLIPDWLLMNKNFIRFDNTVPD 422 +++L +LF YGI+G YT +D + N+ +L W+ N++ N Sbjct: 374 DKDLINLFDYGIEGKHYTKVSDNVIDFPQGVDATNSGYNLQLGWMFGNQSLSYIWNGDSP 433 Query: 423 EFIAEYKAWDDGAIISKGAGFNFDNTPVKNEETKLNGVFTEYLAPIGSGFSDYDTAFPKA 482 + E ++ A SK GF F+ VK+E + V +Y + +G D +T PK Sbjct: 434 DLWNELMTFNKNATKSKAMGFIFNADAVKSEIAAVTNVTNQYKVALETGTVDPETQLPKF 493 Query: 483 LDRLKAAGIDKYIAEYQKQFSEWYA 507 +D+LKAAGIDK IAE QKQ W A Sbjct: 494 IDQLKAAGIDKIIAEKQKQLDAWAA 518 >B3KBX6.1 B3KBX6_9BACI Extracellular solute-binding protein family 1 Length = 508 Score = 149 bits (376), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 145/526 (27%), Positives = 238/526 (45%), Gaps = 46/526 (8%) Query: 1 MKKKKGWLSLLLAAGFLVGGCSQGNNESAAPSSGSGNEGTPSVTDNGSKDGSPLAPAKLK 60 MK K GW SLLLA ++ C GN+ ++GS ++G T +G+ D AP + Sbjct: 1 MKNKWGWFSLLLALMVMISACG-GNDSKGNSAAGSKDDGA---TSSGASD----APYE-- 50 Query: 61 IVLYG----DESNRMKELAKTEFYDVIKKEINAEVEFHFLPWTEYG-GGKTDLMLSTGED 115 V+Y D +L + E + K++IN V+ LP + + +LM+S+GE Sbjct: 51 -VVYAYPTFDSVPADIQLVQDEINKIAKEKINVTVKL--LPISNSAFANQVNLMMSSGEK 107 Query: 116 FATYTDANYMVKSVAKG-LYADLTPYVSAAADLSKNVDEASFKAFQLDGKQYAIPVGNKP 174 VAKG LY A + + + A ++ G Y +P + Sbjct: 108 LDMIAMLGNYSTQVAKGVLYPIGNIMEKNAQGIIDVLGTDYYNATKVGGVSYGVP--SIR 165 Query: 175 NSAEFYSALVRQDLLEEAG--MTQVTSLKELEQFYDKVHAIHPELIGYANTDARRILQYD 232 + A+ Y ++R+DL+++ + QV + + E + + P + G AN I+ Sbjct: 166 DLAQDYGFVMRKDLVDKHNIDLGQVQTFDDFENIFKIIKENEPGMAGAANLSNGSIMS-T 224 Query: 233 YTDKNLYWQNDFIALDESAKDDKIISWFETEEFKKYANLMRGWYEKGIIPKYAATNVPQL 292 Y + N + L+ D K+++ F +EE++ +R WY G + K AAT+ Sbjct: 225 YAIHDPLGNNLGVLLNNGLDDLKVVNLFASEEYEHILRKLREWYLAGYLMKDAATSPEST 284 Query: 293 QSDWNSGKAMFWAGTAARPFEGAATISQAVPEAKLVNYFLGSGR---PKISRGTYS-TAF 348 ++GKA W FE V E++ V + + R P T + A Sbjct: 285 NVLVSNGKAAGWMSAMKPGFE--------VQESRNVGMEMTAVRTLQPVAKTDTVTNVAV 336 Query: 349 FVSTAAKDPERYVMFFNLLQKNQELYDLFAYGIKGTDYTVDENGRLTKINTDSLIPD--- 405 V++ +++PE+ + F NLL ++E+ +L +GI+G Y E D + D Sbjct: 337 GVTSNSENPEKALQFMNLLYTDKEIVNLMVWGIEGKHYVKVEGQDDMIRYPDGVTSDNVG 396 Query: 406 -----WLLMNKNFIRFDNTVPDEFIAE-YKAWDDGAIISKGAGFNFDNTPVKNEETKLNG 459 W+ N+ F+ + D I E ++ A SK GF+FD+ PVKNE N Sbjct: 397 YKNYGWMHGNQ-FLTYVFEGDDPGIYEQMDEFNKSAAKSKALGFSFDSEPVKNEVAACNS 455 Query: 460 VFTEYLAPIGSGFSDYDTAFPKALDRLKAAGIDKYIAEYQKQFSEW 505 V +Y + SG D + A P+ L++L+ AGI+K I E QKQ EW Sbjct: 456 VLEQYRRALESGILDVEKALPEFLNKLENAGIEKIIEEKQKQLDEW 501 >B1DD38.1 B1DD38_9BACL Extracellular solute-binding protein family 1 Length = 515 Score = 135 bits (341), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 133/470 (28%), Positives = 227/470 (48%), Gaps = 33/470 (7%) Query: 54 LAPAKLKIVLYG----DESNRMKELAKTEFYDVIKKEINAEVEFHFLPWTEYGGGKTDLM 109 L P KLK+V G DE+ L K ++ ++INA ++ + W + K +LM Sbjct: 59 LKPYKLKLVYEGPPQADEAMVEDALNK-----ILTEKINATIDIAPIDWGAWDD-KVNLM 112 Query: 110 LSTGE--DFATYTDANYMVKSVAKGLYADLTPYVSA-AADLSKNVDEASFKAFQLDGKQY 166 +++ E D N K+VAKG Y +L + + ++D + + +++GK Y Sbjct: 113 IASREPVDILFTAQWNGYSKNVAKGAYKELGDLLGKYGQGIIGSLDPSFLEGSKINGKNY 172 Query: 167 AIPVGNKPNSAEFYSALV-RQDLLEEAG--MTQVTSLKELEQFYDKVHAIHPELIGYANT 223 IP + F +V R+D+ +E G M+ V + ++L+ Y V P++ Sbjct: 173 GIPTNKE---LAFAGGVVYRKDIADELGLDMSTVKTAEDLDAIYKVVKEKKPDMTPLYMQ 229 Query: 224 DAR--RILQYDYTDKNLYWQNDFIALDESAKDDKIISWFETEEFKKYANLMRGWYEKGII 281 ++ ++D I+ ++S K++ F+ +K+Y L R +++KG I Sbjct: 230 GGSFGQLTEFDSLGDGTI--PGVISKEDSDTTVKMMEDFDI--YKRYLKLARDFFQKGYI 285 Query: 282 PKYAATNVPQLQS-DWNSGKAMFWAGTAARPFEGAATISQAVPEAKLVNYFLGSGRPKIS 340 K AAT QL S D G +F +P + A + A KL +L +G+ + Sbjct: 286 NKDAATT--QLSSGDAYKGGNVFSTIEPLKPGKAAEIANAAGIGGKLDQIYL-TGKTVST 342 Query: 341 RGTYSTAFFVSTAAKDPERYVMFFNLLQKNQELYDLFAYGIKGTDYTVDENGRLTKINTD 400 T +ST +K+PER +MF NLL ++++ +L +GI+G YT+ + + Sbjct: 343 SETAGAMLAISTTSKNPERAMMFINLLHTDKDIVNLLNFGIEGVHYTLSGDVMTPTDKSG 402 Query: 401 SLIPD--WLLMNKNFIRFDNT-VPDEFIAEYKAWDDGAIISKGAGFNFDNTPVKNEETKL 457 P W L N+ NT PD++ ++KA+++GA S G GF F++ VK+E L Sbjct: 403 QYAPGVAWELGNQFLNHVWNTEAPDKW-EQFKAFNEGAKPSPGLGFVFNSDSVKSEVGAL 461 Query: 458 NGVFTEYLAPIGSGFSDYDTAFPKALDRLKAAGIDKYIAEYQKQFSEWYA 507 V +Y + +G D D P+ + LK+AG++K IAE QKQF + A Sbjct: 462 ANVIKQYQKALETGSVDTDKVLPEYIAALKSAGVEKVIAEKQKQFDAFLA 511 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,258,994,273 Number of extensions: 147546632 Number of successful extensions: 305942 Number of sequences better than 1.0e-05: 169 Number of HSP's gapped: 305566 Number of HSP's successfully gapped: 173 Length of query: 510 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 370 Effective length of database: 1,741,754,103 Effective search space: 644449018110 Effective search space used: 644449018110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)