BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0023.p (365 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJD3.1 B3KJD3_9BACI Oxidoreductase domain protein 585 e-165 B7R6U1.1 B7R6U1_9THEO Oxidoreductase family, NAD-binding Rossman... 514 e-144 Q8R8P4.1 Q8R8P4_THETN Predicted dehydrogenases and related proteins 514 e-144 Q929K9.1 Q929K9_LISIN Lin2266 protein 511 e-143 Q71XK3.1 Q71XK3_LISMF Oxidoreductase, Gfo/Idh/MocA family 507 e-142 >B3KJD3.1 B3KJD3_9BACI Oxidoreductase domain protein Length = 361 Score = 585 bits (1507), Expect = e-165, Method: Compositional matrix adjust. Identities = 271/360 (75%), Positives = 309/360 (85%) Query: 1 MSNKLKXXXXXXXXXXXXKHMPSLSRQNNAEMVAFCDIIEERAQEAAKTYGTEDAAVYTD 60 MSN LK KHMPSL +Q AE+VAFCDI+EERAQ+AA YG E A VYTD Sbjct: 1 MSNTLKIGIIGCGGIANGKHMPSLKKQAQAELVAFCDIVEERAQKAAAEYGVEGAKVYTD 60 Query: 61 FREMLAAGGFDIVHVCTPNDSHSVISIAALEAGNHVMCEKPMAKTTAQAQEMLEAARRTG 120 FRE+L G D++HVCTPNDSHS I++A+LEAG HVMCEKPMAKT ++AQ ML+AA+RTG Sbjct: 61 FRELLKDGSIDVIHVCTPNDSHSEITVASLEAGKHVMCEKPMAKTVSEAQAMLDAAKRTG 120 Query: 121 KKLSIAYQNRYRSDSEYLKGLCESGELGDIYYGKAIALRRRAVPTWGVFLDEEKQGGGPL 180 KKL+I Y NR+R DS YLKG+CE GELG+IYYGKA+A+RRRAVPTWGVFLDEEKQGGGPL Sbjct: 121 KKLTIGYNNRFRDDSMYLKGICEEGELGEIYYGKALAIRRRAVPTWGVFLDEEKQGGGPL 180 Query: 181 IDIGTHALDLTLWLMDNYKPRMVVGSTFHKLGQKKNAANAFGPWDPEQFKVEDSAFGFIT 240 IDIGTHALDLTLWLMDNYKP+ V+GSTFHKLGQ++NAANAFGPWDP++F VEDSAFGFIT Sbjct: 181 IDIGTHALDLTLWLMDNYKPKSVLGSTFHKLGQRENAANAFGPWDPKEFTVEDSAFGFIT 240 Query: 241 MENGATISLESSWALNVSEFREAQTLLAGTEGGADMKDGLRLNGERAGRLYETKVDLSSG 300 MENGATI LESSWALNV E EA+ +LAGTEGGADMKDGLR+NGE+ RLYETKVDL++G Sbjct: 241 MENGATIVLESSWALNVLEIGEAKAVLAGTEGGADMKDGLRINGEKMSRLYETKVDLNAG 300 Query: 301 GVAFYSGGAESXXXXEARLWLEAVSEDKEPVVKPEQALVVTQILEAIYESARTGRAVYFD 360 GVAFYSG E+ EAR+WLEA+ EDK+PVVKPEQA VVTQILEAIYESA+TG+AVYFD Sbjct: 301 GVAFYSGSKENDSDREARMWLEAIIEDKDPVVKPEQAFVVTQILEAIYESAKTGKAVYFD 360 >B7R6U1.1 B7R6U1_9THEO Oxidoreductase family, NAD-binding Rossmann fold protein Length = 362 Score = 514 bits (1324), Expect = e-144, Method: Compositional matrix adjust. Identities = 236/342 (69%), Positives = 285/342 (83%) Query: 19 KHMPSLSRQNNAEMVAFCDIIEERAQEAAKTYGTEDAAVYTDFREMLAAGGFDIVHVCTP 78 KHMPSL++ +N EMVAFCDIIEERA++AAK YGTEDA VYTD+R++L D+VHVCTP Sbjct: 20 KHMPSLAKLDNVEMVAFCDIIEERARKAAKEYGTEDAKVYTDYRKLLEDKTIDVVHVCTP 79 Query: 79 NDSHSVISIAALEAGNHVMCEKPMAKTTAQAQEMLEAARRTGKKLSIAYQNRYRSDSEYL 138 N SH+ I++ AL AG HVMCEKPMAKT A++M+EA ++TGKKL+I YQNR+R DS+YL Sbjct: 80 NKSHADITVDALYAGKHVMCEKPMAKTAEGARKMVEAYKKTGKKLTIGYQNRFRPDSQYL 139 Query: 139 KGLCESGELGDIYYGKAIALRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWLMDNY 198 +C++GELG+IY+ KA A+RRRAVPTWGVFLDEE+QGGGPLIDIGTH+LDLTLW+M+NY Sbjct: 140 HKICQNGELGEIYFAKAYAVRRRAVPTWGVFLDEEEQGGGPLIDIGTHSLDLTLWMMNNY 199 Query: 199 KPRMVVGSTFHKLGQKKNAANAFGPWDPEQFKVEDSAFGFITMENGATISLESSWALNVS 258 KPRMVVG+ +HKLG+ NAANA+GPWDPE+F VEDSAFGFI MENGATI LESSWALN Sbjct: 200 KPRMVVGTVYHKLGKMANAANAWGPWDPEKFTVEDSAFGFIVMENGATIILESSWALNTL 259 Query: 259 EFREAQTLLAGTEGGADMKDGLRLNGERAGRLYETKVDLSSGGVAFYSGGAESXXXXEAR 318 + EA T L GTEGGADM+DGLR+NGER GRLY TKV GGVA+Y G E EA+ Sbjct: 260 DVGEAMTTLCGTEGGADMRDGLRINGERFGRLYTTKVSFDIGGVAYYEGKGEDPGYLEAK 319 Query: 319 LWLEAVSEDKEPVVKPEQALVVTQILEAIYESARTGRAVYFD 360 W++A+ D+EPVVKPE+ALVV++ILEAIY SA+TG VYF+ Sbjct: 320 SWIDAIVNDREPVVKPEEALVVSEILEAIYTSAKTGEPVYFN 361 >Q8R8P4.1 Q8R8P4_THETN Predicted dehydrogenases and related proteins Length = 362 Score = 514 bits (1323), Expect = e-144, Method: Compositional matrix adjust. Identities = 234/342 (68%), Positives = 286/342 (83%) Query: 19 KHMPSLSRQNNAEMVAFCDIIEERAQEAAKTYGTEDAAVYTDFREMLAAGGFDIVHVCTP 78 KHMPSL++ +N EMVAFCDIIEERA++AAK YGT+DA VYTD+R++L D+VHVCTP Sbjct: 20 KHMPSLAKLDNVEMVAFCDIIEERARKAAKEYGTDDAKVYTDYRKLLEDKTIDVVHVCTP 79 Query: 79 NDSHSVISIAALEAGNHVMCEKPMAKTTAQAQEMLEAARRTGKKLSIAYQNRYRSDSEYL 138 N SH+ I++ AL AG HVMCEKPMAKT A++M+EA ++TGKKL+I YQNR+R DS+YL Sbjct: 80 NKSHAGITVDALYAGKHVMCEKPMAKTAEGARKMVEAYKKTGKKLTIGYQNRFRPDSQYL 139 Query: 139 KGLCESGELGDIYYGKAIALRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWLMDNY 198 +C++GELG++Y+ KA A+RRRAVPTWGVFLDEE+QGGGPLIDIGTH+LDLTLW+M+NY Sbjct: 140 HKICQNGELGEVYFAKAYAVRRRAVPTWGVFLDEEEQGGGPLIDIGTHSLDLTLWMMNNY 199 Query: 199 KPRMVVGSTFHKLGQKKNAANAFGPWDPEQFKVEDSAFGFITMENGATISLESSWALNVS 258 KPRMVVG+ +HKLG+ NAANA+GPWDPE+F VEDSAFGFI MENGATI LESSWALN Sbjct: 200 KPRMVVGTVYHKLGKMANAANAWGPWDPEKFTVEDSAFGFIVMENGATIILESSWALNTL 259 Query: 259 EFREAQTLLAGTEGGADMKDGLRLNGERAGRLYETKVDLSSGGVAFYSGGAESXXXXEAR 318 + EA T L GTEGGADM+DGLR+NGER GRLY TKV GGVA+Y G E EA+ Sbjct: 260 DVGEAMTTLCGTEGGADMRDGLRINGERFGRLYTTKVSFDIGGVAYYEGKGEDPGYLEAK 319 Query: 319 LWLEAVSEDKEPVVKPEQALVVTQILEAIYESARTGRAVYFD 360 W++A+ D+EPVVKPE+ALVV++ILEAIY SA+TG+ VYF+ Sbjct: 320 SWIDAIVNDREPVVKPEEALVVSEILEAIYTSAKTGKPVYFN 361 >Q929K9.1 Q929K9_LISIN Lin2266 protein Length = 358 Score = 511 bits (1315), Expect = e-143, Method: Compositional matrix adjust. Identities = 235/342 (68%), Positives = 285/342 (83%) Query: 19 KHMPSLSRQNNAEMVAFCDIIEERAQEAAKTYGTEDAAVYTDFREMLAAGGFDIVHVCTP 78 KHMPSL + AEMVAFCDI+ E+A+ AAK +G+E+A+VYT++ E+L D++HVCTP Sbjct: 17 KHMPSLLKAEKAEMVAFCDIVLEKAEAAAKEFGSENASVYTNYVELLQDKSIDVIHVCTP 76 Query: 79 NDSHSVISIAALEAGNHVMCEKPMAKTTAQAQEMLEAARRTGKKLSIAYQNRYRSDSEYL 138 N SH+ ISIAA+EAG HVMCEKPMAKTTA+A+ M+EAA RTGKKL+I YQNR+R DS+YL Sbjct: 77 NISHAEISIAAMEAGKHVMCEKPMAKTTAEAKSMIEAANRTGKKLTIGYQNRFRKDSDYL 136 Query: 139 KGLCESGELGDIYYGKAIALRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWLMDNY 198 +CE+GELGDIYY KA A+RRRAVPTWGVFLDEE QGGGPLIDIGTHALDLTLW+MDNY Sbjct: 137 HQVCENGELGDIYYAKAKAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNY 196 Query: 199 KPRMVVGSTFHKLGQKKNAANAFGPWDPEQFKVEDSAFGFITMENGATISLESSWALNVS 258 KP+ VVG+++HKL +K+NAANA+G WDP +F VEDSAFGFITMENGATI LE+SWALN Sbjct: 197 KPKYVVGNSYHKLAKKENAANAWGSWDPSKFTVEDSAFGFITMENGATIVLEASWALNTL 256 Query: 259 EFREAQTLLAGTEGGADMKDGLRLNGERAGRLYETKVDLSSGGVAFYSGGAESXXXXEAR 318 + EA+T L+GTEGGADM+DGLR+NGE ++YE K+ L +GGV FY G + EA Sbjct: 257 DVGEARTSLSGTEGGADMEDGLRINGEAHSQMYEKKIQLEAGGVDFYDGTGDDPALIEAE 316 Query: 319 LWLEAVSEDKEPVVKPEQALVVTQILEAIYESARTGRAVYFD 360 WLEA+ D +PVVKPEQALVVTQILEAIYES++TG+ VYF+ Sbjct: 317 QWLEAILNDTDPVVKPEQALVVTQILEAIYESSKTGQPVYFN 358 >Q71XK3.1 Q71XK3_LISMF Oxidoreductase, Gfo/Idh/MocA family Length = 358 Score = 507 bits (1305), Expect = e-142, Method: Compositional matrix adjust. Identities = 233/342 (68%), Positives = 284/342 (83%) Query: 19 KHMPSLSRQNNAEMVAFCDIIEERAQEAAKTYGTEDAAVYTDFREMLAAGGFDIVHVCTP 78 KHMPSL + AEMVAFCDI+ E+A+ AAK +G+E+A+VYT + E+L D++HVCTP Sbjct: 17 KHMPSLLKAEKAEMVAFCDIVLEKAEAAAKEFGSENASVYTSYIELLQDKSIDVIHVCTP 76 Query: 79 NDSHSVISIAALEAGNHVMCEKPMAKTTAQAQEMLEAARRTGKKLSIAYQNRYRSDSEYL 138 N SH+ ISIAA+EAG HVMCEKPMAKTT +A+ M+EAA RTGKKL+I YQNR+R DS+YL Sbjct: 77 NISHAEISIAAMEAGKHVMCEKPMAKTTEEAKSMIEAANRTGKKLTIGYQNRFRKDSDYL 136 Query: 139 KGLCESGELGDIYYGKAIALRRRAVPTWGVFLDEEKQGGGPLIDIGTHALDLTLWLMDNY 198 +CE+GELGDIYY KA A+RRRAVPTWGVFLDEE QGGGPLIDIGTHALDLTLW+MDNY Sbjct: 137 HQVCENGELGDIYYAKAKAIRRRAVPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNY 196 Query: 199 KPRMVVGSTFHKLGQKKNAANAFGPWDPEQFKVEDSAFGFITMENGATISLESSWALNVS 258 KP+ VVG+++HKL +K+NAANA+G WDP +F VEDSAFGFITMENGATI LE+SWALN Sbjct: 197 KPKYVVGNSYHKLAKKENAANAWGSWDPNKFTVEDSAFGFITMENGATIVLEASWALNTL 256 Query: 259 EFREAQTLLAGTEGGADMKDGLRLNGERAGRLYETKVDLSSGGVAFYSGGAESXXXXEAR 318 + EA+T L+GTEGGADM+DGLR+NGE ++YE K+ L +GGV FY G + EA Sbjct: 257 DVGEARTSLSGTEGGADMEDGLRINGEAHSQMYEKKIQLEAGGVDFYDGTGDDPALIEAE 316 Query: 319 LWLEAVSEDKEPVVKPEQALVVTQILEAIYESARTGRAVYFD 360 WLEA+ ++ +PVVKPEQALVVTQILEAIYES++TG+ VYF+ Sbjct: 317 QWLEAIEKNTDPVVKPEQALVVTQILEAIYESSKTGQPVYFN 358 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,039,230,084 Number of extensions: 81948466 Number of successful extensions: 185353 Number of sequences better than 1.0e-05: 4305 Number of HSP's gapped: 182560 Number of HSP's successfully gapped: 4305 Length of query: 365 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 228 Effective length of database: 1,770,017,148 Effective search space: 403563909744 Effective search space used: 403563909744 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)