BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0022.p (357 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ88.1 B3KJ88_9BACI Oxidoreductase domain protein 528 e-148 Q4ESY0.1 Q4ESY0_LISMO Oxidoreductase, Gfo/Idh/MocA family 458 e-127 A0AKR1.1 A0AKR1_LISW6 Complete genome 457 e-127 Q929L3.1 Q929L3_LISIN Lin2262 protein 457 e-127 B8DFF3.1 B8DFF3_LISMH Oxidoreductase, Gfo/Idh/MocA family 457 e-127 >B3KJ88.1 B3KJ88_9BACI Oxidoreductase domain protein Length = 358 Score = 528 bits (1359), Expect = e-148, Method: Compositional matrix adjust. Identities = 247/355 (69%), Positives = 288/355 (81%) Query: 3 TMSSHKHTIVIVGYGGMGSYHTQLIEENGRLEVAGTFDLLEERRSASEAAGYTAYASYEE 62 T K IVIVGYGGMGSYH +LI++N LEV GT+D+LEERR AS GY+AY SYE+ Sbjct: 2 TTEIRKQAIVIVGYGGMGSYHAKLIKDNAFLEVTGTYDILEERRKASREDGYSAYESYEQ 61 Query: 63 VLADPKVGTILIATPNDVHKEIALAAFRAGKHVICEKPVAMSSDEFIEMTQAAESAGRVL 122 VL D V +LIATPNDVHKE+AL AF AGKHVICEKPV +S +F +M AA++AGRVL Sbjct: 62 VLNDAAVDVVLIATPNDVHKELALQAFEAGKHVICEKPVTLSVSDFKDMVSAADAAGRVL 121 Query: 123 MVHQNRRWDEDFRVIKEMYDHGTIGSLFQLESRVHGANGIPGDWRHVKAQGGGMLLDWGV 182 MVHQNRRWDEDF IK+MY+ TIG LF++ESRVHGANGIPGDWRH++A GGGMLLDWGV Sbjct: 122 MVHQNRRWDEDFLTIKQMYNEKTIGDLFRIESRVHGANGIPGDWRHLEAHGGGMLLDWGV 181 Query: 183 HLLDQLLFMIDXXXXXXXXXXXFILGNEVDDGFEAVLQFENGVKAIVEVGTTNFITLPRW 242 HLLDQ+LFM+D FILG+EVDDGFE+V++FENGV ++EVGTTNFITLPRW Sbjct: 182 HLLDQILFMVDSRMTSLSSRLSFILGDEVDDGFESVIEFENGVSVLIEVGTTNFITLPRW 241 Query: 243 YVKGTEGTGVIEDWSLTGRIVTRNQESEKLEPTPIRAGVGLTKTMAPPSEGATITEALPP 302 YVKGTEGTG+IEDWSL GR+VTRNQ++ K+EP PI+AGVGLTKTMAPPSE ATIT ALP Sbjct: 242 YVKGTEGTGIIEDWSLNGRVVTRNQDAAKVEPKPIQAGVGLTKTMAPPSEEATITGALPE 301 Query: 303 AAELASSFYTNFADVIEGTAEPIVKNPEVLRVLKLIEAIFAAAETNQTIKDFDLY 357 +SSF+ NFA V G AEPIVKN EVLRVL LIEA+F +A T +T+KDFD+Y Sbjct: 302 PYRQSSSFFDNFAAVTRGEAEPIVKNEEVLRVLNLIEAVFESARTGETLKDFDIY 356 >Q4ESY0.1 Q4ESY0_LISMO Oxidoreductase, Gfo/Idh/MocA family Length = 349 Score = 458 bits (1178), Expect = e-127, Method: Compositional matrix adjust. Identities = 216/344 (62%), Positives = 257/344 (74%) Query: 8 KHTIVIVGYGGMGSYHTQLIEENGRLEVAGTFDLLEERRSASEAAGYTAYASYEEVLADP 67 K+ +VIVGYGGMGSYH L LEV G FD+LEE+R A+ G Y S+E VLAD Sbjct: 3 KYQLVIVGYGGMGSYHVTLASAADNLEVHGVFDILEEKREAAAQKGLKIYESFEAVLADE 62 Query: 68 KVGTILIATPNDVHKEIALAAFRAGKHVICEKPVAMSSDEFIEMTQAAESAGRVLMVHQN 127 KV +LIATPND HKE+A+ A AGKHV+CEKPV M+S++ + + A+ + MVHQN Sbjct: 63 KVDAVLIATPNDSHKELAITALEAGKHVVCEKPVTMTSEDLLAIMDVAKRVNKHFMVHQN 122 Query: 128 RRWDEDFRVIKEMYDHGTIGSLFQLESRVHGANGIPGDWRHVKAQGGGMLLDWGVHLLDQ 187 RRWDEDF +IKEM++ TIG +F LESRVHGANGIPGDWRH+KA GGGM+LDWGVHLLDQ Sbjct: 123 RRWDEDFLIIKEMFEQKTIGEMFHLESRVHGANGIPGDWRHLKAHGGGMVLDWGVHLLDQ 182 Query: 188 LLFMIDXXXXXXXXXXXFILGNEVDDGFEAVLQFENGVKAIVEVGTTNFITLPRWYVKGT 247 LLF++D F LG+EVDDGF + FENG+ A +EVGTTNFI LPRWYVKGT Sbjct: 183 LLFLVDSNVKSVSANLSFALGDEVDDGFVTFITFENGITAQIEVGTTNFIKLPRWYVKGT 242 Query: 248 EGTGVIEDWSLTGRIVTRNQESEKLEPTPIRAGVGLTKTMAPPSEGATITEALPPAAELA 307 EGTG+I DW L+G IV ++ EPTPI+AG GLTKTMAPPSE AT T +LP A+LA Sbjct: 243 EGTGIIHDWDLSGEIVQPTALAKTSEPTPIKAGQGLTKTMAPPSEEATNTLSLPAPAKLA 302 Query: 308 SSFYTNFADVIEGTAEPIVKNPEVLRVLKLIEAIFAAAETNQTI 351 SFY NF DV+ T+EPIV+N EV +VLKLIEAIF AAETN+TI Sbjct: 303 PSFYNNFVDVLNNTSEPIVQNEEVYQVLKLIEAIFEAAETNRTI 346 >A0AKR1.1 A0AKR1_LISW6 Complete genome Length = 349 Score = 457 bits (1177), Expect = e-127, Method: Compositional matrix adjust. Identities = 213/344 (61%), Positives = 261/344 (75%) Query: 8 KHTIVIVGYGGMGSYHTQLIEENGRLEVAGTFDLLEERRSASEAAGYTAYASYEEVLADP 67 K+ +VIVGYGGMGSYH L +EV G FD+LEE+R A+ G Y S+ +VLAD Sbjct: 3 KYQLVIVGYGGMGSYHVTLASAADNIEVHGVFDILEEKRDAAAQKGLNIYESFADVLADE 62 Query: 68 KVGTILIATPNDVHKEIALAAFRAGKHVICEKPVAMSSDEFIEMTQAAESAGRVLMVHQN 127 KV +LIATPND HKE+A++A AGKHV+CEKPV M+SD+ + + AA+ + MVHQN Sbjct: 63 KVDAVLIATPNDSHKELAISALEAGKHVVCEKPVTMTSDDLLAIMDAAKKVNKHFMVHQN 122 Query: 128 RRWDEDFRVIKEMYDHGTIGSLFQLESRVHGANGIPGDWRHVKAQGGGMLLDWGVHLLDQ 187 RRWDEDF +IKEM++ TIG +F LESRVHGANGIPGDWRH+KA GGGM+LDWGVHLLDQ Sbjct: 123 RRWDEDFLIIKEMFEQKTIGDMFHLESRVHGANGIPGDWRHLKAHGGGMVLDWGVHLLDQ 182 Query: 188 LLFMIDXXXXXXXXXXXFILGNEVDDGFEAVLQFENGVKAIVEVGTTNFITLPRWYVKGT 247 LLF++D + LG+EVDDGF + FENG+ A +EVGTTNFI LPRWYVKGT Sbjct: 183 LLFLVDSNVKSVSAHLSYALGDEVDDGFVTFITFENGITAQIEVGTTNFIKLPRWYVKGT 242 Query: 248 EGTGVIEDWSLTGRIVTRNQESEKLEPTPIRAGVGLTKTMAPPSEGATITEALPPAAELA 307 EGTG+I DW L+G IV ++ EPTPI+AG GLTKTMAPPSE AT T +LP A+LA Sbjct: 243 EGTGIIHDWDLSGEIVKPTALAKTSEPTPIKAGQGLTKTMAPPSEEATNTLSLPTPAKLA 302 Query: 308 SSFYTNFADVIEGTAEPIVKNPEVLRVLKLIEAIFAAAETNQTI 351 +SFY NFA+V+ G ++PIV+N EV +VLKLIEAIF AAET++TI Sbjct: 303 TSFYNNFANVLSGESDPIVQNEEVYQVLKLIEAIFEAAETDKTI 346 >Q929L3.1 Q929L3_LISIN Lin2262 protein Length = 349 Score = 457 bits (1176), Expect = e-127, Method: Compositional matrix adjust. Identities = 215/344 (62%), Positives = 257/344 (74%) Query: 8 KHTIVIVGYGGMGSYHTQLIEENGRLEVAGTFDLLEERRSASEAAGYTAYASYEEVLADP 67 K+ +VIVGYGGMGSYH L LEV G FD+L E+R A+ G Y SYE VLAD Sbjct: 3 KYQLVIVGYGGMGSYHVTLASAADNLEVHGVFDILAEKREAAAQKGLKIYESYEAVLADE 62 Query: 68 KVGTILIATPNDVHKEIALAAFRAGKHVICEKPVAMSSDEFIEMTQAAESAGRVLMVHQN 127 KV +LIATPND HKE+A++A AGKHV+CEKPV M+S++ + + A+ + MVHQN Sbjct: 63 KVDAVLIATPNDSHKELAISALEAGKHVVCEKPVTMTSEDLLAIMDVAKRVNKHFMVHQN 122 Query: 128 RRWDEDFRVIKEMYDHGTIGSLFQLESRVHGANGIPGDWRHVKAQGGGMLLDWGVHLLDQ 187 RRWDEDF +IKEM++ TIG +F LESRVHGANGIPGDWRH+KA GGGM+LDWGVHLLDQ Sbjct: 123 RRWDEDFLIIKEMFEQKTIGEMFHLESRVHGANGIPGDWRHLKAHGGGMVLDWGVHLLDQ 182 Query: 188 LLFMIDXXXXXXXXXXXFILGNEVDDGFEAVLQFENGVKAIVEVGTTNFITLPRWYVKGT 247 LLF++D F LG+EVDDGF + FENG+ A +EVGTTNFI LPRWYVKGT Sbjct: 183 LLFLVDSNVKSVSANLSFALGDEVDDGFVTFITFENGITAQIEVGTTNFIKLPRWYVKGT 242 Query: 248 EGTGVIEDWSLTGRIVTRNQESEKLEPTPIRAGVGLTKTMAPPSEGATITEALPPAAELA 307 EGTG+I DW L+G IV ++ EPTPI+AG GLTKTMAPPSE AT T +LP A+LA Sbjct: 243 EGTGIIHDWDLSGEIVKPTALAKTSEPTPIKAGQGLTKTMAPPSEEATNTLSLPAPAKLA 302 Query: 308 SSFYTNFADVIEGTAEPIVKNPEVLRVLKLIEAIFAAAETNQTI 351 SFY NF DV+ T+EPIV+N EV +VLKLIEAIF AAETN+T+ Sbjct: 303 PSFYNNFVDVLNNTSEPIVQNEEVYQVLKLIEAIFEAAETNRTV 346 >B8DFF3.1 B8DFF3_LISMH Oxidoreductase, Gfo/Idh/MocA family Length = 349 Score = 457 bits (1175), Expect = e-127, Method: Compositional matrix adjust. Identities = 215/344 (62%), Positives = 257/344 (74%) Query: 8 KHTIVIVGYGGMGSYHTQLIEENGRLEVAGTFDLLEERRSASEAAGYTAYASYEEVLADP 67 K+ +VIVGYGGMGSYH L LEV G FD+LEE+R A+ G Y S+E VLAD Sbjct: 3 KYQLVIVGYGGMGSYHVTLASAADNLEVHGVFDILEEKREAAAEKGLKIYESFEAVLADE 62 Query: 68 KVGTILIATPNDVHKEIALAAFRAGKHVICEKPVAMSSDEFIEMTQAAESAGRVLMVHQN 127 +V +LIATPND HKE+A+ A AGKHV+CEKPV M+S++ + + A+ + MVHQN Sbjct: 63 QVDAVLIATPNDSHKELAITALEAGKHVVCEKPVTMTSEDLLAIMDVAKKVNKHFMVHQN 122 Query: 128 RRWDEDFRVIKEMYDHGTIGSLFQLESRVHGANGIPGDWRHVKAQGGGMLLDWGVHLLDQ 187 RRWDEDF +IKEM++ TIG +F LESRVHGANGIPGDWRH+KA GGGM+LDWGVHLLDQ Sbjct: 123 RRWDEDFLIIKEMFEQKTIGEMFHLESRVHGANGIPGDWRHLKAHGGGMVLDWGVHLLDQ 182 Query: 188 LLFMIDXXXXXXXXXXXFILGNEVDDGFEAVLQFENGVKAIVEVGTTNFITLPRWYVKGT 247 LLF++D F LG+EVDDGF + FENG+ A +EVGTTNFI LPRWYVKGT Sbjct: 183 LLFLVDSNVKSVSANLSFALGDEVDDGFVTFITFENGITAQIEVGTTNFIKLPRWYVKGT 242 Query: 248 EGTGVIEDWSLTGRIVTRNQESEKLEPTPIRAGVGLTKTMAPPSEGATITEALPPAAELA 307 EGTG+I DW L+G IV ++ EPTPI+AG GLTKTMAPPSE AT T +LP A+LA Sbjct: 243 EGTGIIHDWDLSGEIVKPTALAKTSEPTPIKAGQGLTKTMAPPSEEATNTLSLPAPAKLA 302 Query: 308 SSFYTNFADVIEGTAEPIVKNPEVLRVLKLIEAIFAAAETNQTI 351 SFY NF DV+ T+EPIV+N EV +VLKLIEAIF AAETN+TI Sbjct: 303 PSFYNNFVDVLNNTSEPIVQNEEVYQVLKLIEAIFEAAETNRTI 346 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,071,354,161 Number of extensions: 83968294 Number of successful extensions: 234047 Number of sequences better than 1.0e-05: 4327 Number of HSP's gapped: 231346 Number of HSP's successfully gapped: 4327 Length of query: 357 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 220 Effective length of database: 1,770,017,148 Effective search space: 389403772560 Effective search space used: 389403772560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)