BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0021.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJD4.1 B3KJD4_9BACI Xylose isomerase domain protein TIM barrel 585 e-165 B1DE71.1 B1DE71_9BACL Xylose isomerase domain protein TIM barrel 531 e-149 Q5WJX1.1 Q5WJX1_BACSK Sugar phosphate isomerases/epimerase 473 e-131 Q8ENC4.1 Q8ENC4_OCEIH Hypothetical conserved protein 457 e-127 C0ZD67.1 C0ZD67_BREBN Putative uncharacterized protein 456 e-126 >B3KJD4.1 B3KJD4_9BACI Xylose isomerase domain protein TIM barrel Length = 322 Score = 585 bits (1509), Expect = e-165, Method: Compositional matrix adjust. Identities = 279/322 (86%), Positives = 299/322 (92%) Query: 1 MKLGVFMVLFGGRKLEDALDYVASKGLKAVEIGTGGNPGNSHCDPKLLLENETALKEFKH 60 MKLGVFMVL GRKLEDALDYVASKGLKAVEIGTGG PGN+HC+P LLENETALK FK+ Sbjct: 1 MKLGVFMVLLSGRKLEDALDYVASKGLKAVEIGTGGYPGNAHCNPVELLENETALKNFKN 60 Query: 61 AVESRGLMISALSCHGNPLHPQKELARKDHEDFVNSVKLAQKLGVPVVNTFSGCPGDHED 120 AVESRGL+ISALSCHGNPLHPQK +A++ H+ +V +V+LA+KL VPVVNTFSGCPGDHED Sbjct: 61 AVESRGLIISALSCHGNPLHPQKAIAKEFHDTYVKTVELAEKLEVPVVNTFSGCPGDHED 120 Query: 121 AKYPNWPVAPWPNDYQEILAWQWENKVIPYWTEWGAFAAQHDVKIGLELHGGFSVHTPAT 180 AKYPNWPVAPWPNDYQEIL WQW+NKVIPYWTEWG FAA +VKIGLELHGGFSVHTP T Sbjct: 121 AKYPNWPVAPWPNDYQEILTWQWDNKVIPYWTEWGKFAADRNVKIGLELHGGFSVHTPGT 180 Query: 181 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAIHILGRQGAIHHFHAKDTVIDPVNVNMYG 240 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAI ILGR+GAIHHFHAKDT IDP+NVN +G Sbjct: 181 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAIQILGREGAIHHFHAKDTSIDPINVNKHG 240 Query: 241 LTDMQSYTNMLDRAWQFRSVGYGHDVKVWADIISALRLVGYDYVVSIEHEDGLMSVEEGF 300 LTDMQSY NMLDRAWQFRSVGYGHDVK WAD++SALRLVGYDYVVSIEHEDGLMSVEEGF Sbjct: 241 LTDMQSYANMLDRAWQFRSVGYGHDVKEWADMMSALRLVGYDYVVSIEHEDGLMSVEEGF 300 Query: 301 SKAVHNLRQVLIEEPLAEMWWV 322 SKAV NL+QVLIEEPL EMWWV Sbjct: 301 SKAVQNLQQVLIEEPLGEMWWV 322 >B1DE71.1 B1DE71_9BACL Xylose isomerase domain protein TIM barrel Length = 322 Score = 531 bits (1368), Expect = e-149, Method: Compositional matrix adjust. Identities = 251/322 (77%), Positives = 279/322 (86%) Query: 1 MKLGVFMVLFGGRKLEDALDYVASKGLKAVEIGTGGNPGNSHCDPKLLLENETALKEFKH 60 MKLGVF VLF + E+ALDY+A+KGL+AVE+GTGG PGN+HCDP LL + LK FKH Sbjct: 1 MKLGVFAVLFSQKPFEEALDYIAAKGLEAVELGTGGYPGNAHCDPDTLLADAGKLKAFKH 60 Query: 61 AVESRGLMISALSCHGNPLHPQKELARKDHEDFVNSVKLAQKLGVPVVNTFSGCPGDHED 120 AVESRGLMISALSCH NPLHPQK ++ D +++LA +L VPVVNTFSGCPGDHED Sbjct: 61 AVESRGLMISALSCHANPLHPQKAISSADDATIRKTIELANRLEVPVVNTFSGCPGDHED 120 Query: 121 AKYPNWPVAPWPNDYQEILAWQWENKVIPYWTEWGAFAAQHDVKIGLELHGGFSVHTPAT 180 AKYPNWPVAPWPND+QEILAWQWE KVIPYW+E G A Q+ VKIGLELHGGFSVHTPAT Sbjct: 121 AKYPNWPVAPWPNDFQEILAWQWEKKVIPYWSEIGGIAEQNGVKIGLELHGGFSVHTPAT 180 Query: 181 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAIHILGRQGAIHHFHAKDTVIDPVNVNMYG 240 LLRLREAAG+ IGANLDPSHMWWQGIDPV+AI ILGR+ AIHHFHAKDT ID NVN +G Sbjct: 181 LLRLREAAGKAIGANLDPSHMWWQGIDPVEAIKILGRENAIHHFHAKDTSIDFNNVNKHG 240 Query: 241 LTDMQSYTNMLDRAWQFRSVGYGHDVKVWADIISALRLVGYDYVVSIEHEDGLMSVEEGF 300 +TDMQSYT MLDRAWQFRSVG+GHD+K WADIIS LRLVGYDYVVSIEHEDGLMSVEEGF Sbjct: 241 VTDMQSYTLMLDRAWQFRSVGFGHDLKTWADIISTLRLVGYDYVVSIEHEDGLMSVEEGF 300 Query: 301 SKAVHNLRQVLIEEPLAEMWWV 322 SKAV NL+QVLI+EP+ +MWWV Sbjct: 301 SKAVQNLQQVLIKEPVGDMWWV 322 >Q5WJX1.1 Q5WJX1_BACSK Sugar phosphate isomerases/epimerase Length = 322 Score = 473 bits (1216), Expect = e-131, Method: Compositional matrix adjust. Identities = 221/322 (68%), Positives = 260/322 (80%) Query: 1 MKLGVFMVLFGGRKLEDALDYVASKGLKAVEIGTGGNPGNSHCDPKLLLENETALKEFKH 60 MKLGVF VLF + E+ LD+V + GL+ +EIGTGG PGN+HC+P LLENET LK FK Sbjct: 1 MKLGVFTVLFSEKPFEEMLDHVKALGLETIEIGTGGYPGNAHCNPGELLENETKLKAFKK 60 Query: 61 AVESRGLMISALSCHGNPLHPQKELARKDHEDFVNSVKLAQKLGVPVVNTFSGCPGDHED 120 AVE RGL IS+LSCHGN L P + A + H+ FV +V+LA+KL VP V TFSG PG +E Sbjct: 61 AVEDRGLTISSLSCHGNALTPDADFANQSHDAFVKTVQLAEKLEVPTVTTFSGTPGAYEG 120 Query: 121 AKYPNWPVAPWPNDYQEILAWQWENKVIPYWTEWGAFAAQHDVKIGLELHGGFSVHTPAT 180 AK P+WPVAPWPN+Y +IL WQW+ K+IPYW+EWG +AA H+VK+ LELHGGF VH+PAT Sbjct: 121 AKQPSWPVAPWPNEYGDILKWQWQEKLIPYWSEWGQYAADHNVKVALELHGGFLVHSPAT 180 Query: 181 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAIHILGRQGAIHHFHAKDTVIDPVNVNMYG 240 LL+LREA GE IGAN+DPSH+WWQGIDPV AI ILG+ AIH FHAKDT ID NVNM+G Sbjct: 181 LLKLREACGEAIGANVDPSHLWWQGIDPVAAIKILGKHNAIHFFHAKDTYIDQENVNMHG 240 Query: 241 LTDMQSYTNMLDRAWQFRSVGYGHDVKVWADIISALRLVGYDYVVSIEHEDGLMSVEEGF 300 L DMQSY NM DRAW FRSVGYGHD+K WADIIS LRLVGYD VSIEHEDGLMS++EGF Sbjct: 241 LLDMQSYANMQDRAWYFRSVGYGHDLKTWADIISTLRLVGYDGAVSIEHEDGLMSIDEGF 300 Query: 301 SKAVHNLRQVLIEEPLAEMWWV 322 KAV NL+QVL++EP +MWW+ Sbjct: 301 QKAVANLQQVLMKEPAPDMWWL 322 >Q8ENC4.1 Q8ENC4_OCEIH Hypothetical conserved protein Length = 322 Score = 457 bits (1176), Expect = e-127, Method: Compositional matrix adjust. Identities = 213/322 (66%), Positives = 249/322 (77%) Query: 1 MKLGVFMVLFGGRKLEDALDYVASKGLKAVEIGTGGNPGNSHCDPKLLLENETALKEFKH 60 MKLGVF VLFG + ++ LDYV GLKAVEIGTGGNPG +HC+ LL+NE K+FK Sbjct: 1 MKLGVFTVLFGEKSFDEMLDYVQESGLKAVEIGTGGNPGTAHCNVDELLDNEEKRKQFKE 60 Query: 61 AVESRGLMISALSCHGNPLHPQKELARKDHEDFVNSVKLAQKLGVPVVNTFSGCPGDHED 120 +++RGL ISALSCH NP+ P KE A+K H+DF +V+LA+ L VPVVNTFSG PG HE Sbjct: 61 KIDARGLTISALSCHDNPISPNKEHAQKAHDDFRKTVRLAELLEVPVVNTFSGTPGSHEG 120 Query: 121 AKYPNWPVAPWPNDYQEILAWQWENKVIPYWTEWGAFAAQHDVKIGLELHGGFSVHTPAT 180 +KYPNWPV PWP +Y +IL WQWE K+IPYW E FA H VKIGLELH GF VHTP T Sbjct: 121 SKYPNWPVTPWPTEYSDILKWQWEEKLIPYWKEQAEFAKNHGVKIGLELHAGFLVHTPYT 180 Query: 181 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAIHILGRQGAIHHFHAKDTVIDPVNVNMYG 240 LL+LREA + IGANLDPSH+WWQGIDPV AI ILG+ GAIHHFHAKDT ID NVNMYG Sbjct: 181 LLKLREATNDAIGANLDPSHLWWQGIDPVAAIKILGKAGAIHHFHAKDTYIDQDNVNMYG 240 Query: 241 LTDMQSYTNMLDRAWQFRSVGYGHDVKVWADIISALRLVGYDYVVSIEHEDGLMSVEEGF 300 LTDMQ Y ++ RAW FRSVGYGH ++ W++I+SALR GYDYV+SIEHED +MS++EGF Sbjct: 241 LTDMQPYGDVQTRAWSFRSVGYGHSIQEWSNIVSALRTYGYDYVISIEHEDPIMSIDEGF 300 Query: 301 SKAVHNLRQVLIEEPLAEMWWV 322 AV NL+ V IEE A MWW Sbjct: 301 QAAVKNLKAVNIEESPATMWWA 322 >C0ZD67.1 C0ZD67_BREBN Putative uncharacterized protein Length = 322 Score = 456 bits (1173), Expect = e-126, Method: Compositional matrix adjust. Identities = 209/322 (64%), Positives = 255/322 (79%) Query: 1 MKLGVFMVLFGGRKLEDALDYVASKGLKAVEIGTGGNPGNSHCDPKLLLENETALKEFKH 60 MKLGVF VLF + E+ LD+V + GL+AVEIGTG PGN+HC+ LLE+ + +K Sbjct: 1 MKLGVFTVLFSEKSFEEMLDHVQAAGLEAVEIGTGCYPGNAHCNLDELLESPEKRRAYKK 60 Query: 61 AVESRGLMISALSCHGNPLHPQKELARKDHEDFVNSVKLAQKLGVPVVNTFSGCPGDHED 120 AVE RGL+ISALSCHGNPL P+K A++ H+ FV +V+LA+ L VPVVN FSG GDHE Sbjct: 61 AVEDRGLIISALSCHGNPLTPEKSFAQQSHDTFVKTVQLAELLEVPVVNCFSGTAGDHEG 120 Query: 121 AKYPNWPVAPWPNDYQEILAWQWENKVIPYWTEWGAFAAQHDVKIGLELHGGFSVHTPAT 180 AKYP+WPVAPWPN+Y+++L WQW K+IPYW EW A+A +H VK+ LELHGGF VHTP T Sbjct: 121 AKYPSWPVAPWPNEYRDVLHWQWNEKLIPYWREWTAYATEHHVKVALELHGGFLVHTPGT 180 Query: 181 LLRLREAAGEVIGANLDPSHMWWQGIDPVQAIHILGRQGAIHHFHAKDTVIDPVNVNMYG 240 LL+LRE GE IGAN DPSHMWWQGIDPV AI ILG++ AI+HFHAKDT ID VNMYG Sbjct: 181 LLKLREQVGEGIGANFDPSHMWWQGIDPVAAIKILGKEKAIYHFHAKDTFIDQEKVNMYG 240 Query: 241 LTDMQSYTNMLDRAWQFRSVGYGHDVKVWADIISALRLVGYDYVVSIEHEDGLMSVEEGF 300 LTDM SY N+ +RAW FR+VGYGH + WAD++SALR+ GYDYVVSIEHED +MS+EEGF Sbjct: 241 LTDMNSYANLHERAWYFRTVGYGHSQQTWADMMSALRMNGYDYVVSIEHEDAIMSIEEGF 300 Query: 301 SKAVHNLRQVLIEEPLAEMWWV 322 +AV NL+QV++ EP+ +WWV Sbjct: 301 QRAVQNLQQVILREPVQNLWWV 322 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,130,825,784 Number of extensions: 94399600 Number of successful extensions: 205479 Number of sequences better than 1.0e-05: 186 Number of HSP's gapped: 205301 Number of HSP's successfully gapped: 186 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)