BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0020.p (326 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DAB9.1 B1DAB9_9BACL Aminoglycoside phosphotransferase 264 1e-68 A9FXX2.1 A9FXX2_SORC5 Probable Aminoglycoside phosphotransferase 241 7e-62 A9AV34.1 A9AV34_HERA2 Aminoglycoside phosphotransferase 231 6e-59 B9LIA9.1 B9LIA9_CHLSY Aminoglycoside phosphotransferase 207 1e-51 A9WDM2.1 A9WDM2_CHLAA Aminoglycoside phosphotransferase 207 1e-51 >B1DAB9.1 B1DAB9_9BACL Aminoglycoside phosphotransferase Length = 320 Score = 264 bits (674), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 138/320 (43%), Positives = 192/320 (60%), Gaps = 5/320 (1%) Query: 1 MESFTKVKLDDQQLRALAASTFGTDTAIISSKELTGGFFNAAYDLELSDGRSVILKVAPA 60 ME+ K +L+D+++ + FG A IS+ ELT G+ N AY + L DGR +LK+AP+ Sbjct: 1 MENANKYRLNDKEIETIVREAFGQ--AYISASELTDGWANMAYSIVLGDGRKTVLKIAPS 58 Query: 61 AETETLSYEKDIMRAEVEALRLVQAAGTVPVPAVYGFDESLELIPSPFFFMEKIEGTPYN 120 A+ + YEK++MR EVEA+RLV +P P VY +D S +IP+ +FFME +EGT N Sbjct: 59 ADKLVMRYEKNMMRTEVEAMRLVADTPGLPAPRVYAYDASCSIIPAEYFFMEYVEGTALN 118 Query: 121 EVKGSLPDEVRASIERELGRYQRLINGIKGERFGLFGQPQDTAARTWRETFTAMIHNVLD 180 +V+ SL E R +I RELG Y R IN K FG QP+ W E F M+ +VL Sbjct: 119 KVRDSLSLEEREAIARELGGYSRSINAYKNSFFGSL-QPEGRRKDNWAEAFEGMMADVLA 177 Query: 181 DGQRLGAVLPASYDEIKQGLEKFLPALEEVTEPRLIHWDLWNGNIFVKDGVIVSIIDWER 240 DG+ G LP SY EI++ + ++ L V E LIHWDLW+GN+FV++G I +ID+ER Sbjct: 178 DGKDAGVELPMSYAEIEKEVLRYRELLGVVKEASLIHWDLWDGNLFVQEGSISGLIDFER 237 Query: 241 ALWGDVLMEYYFRHFENSP--AFFEGYGAAFDSPNERXXXXXXXXXXXXXXVIECYSRQY 298 A WGD L E+YF F + AF EGYG + +E+ VIEC R+Y Sbjct: 238 AFWGDPLSEFYFGRFAKNTLGAFCEGYGITGLTESEQRRRVLYDFYLDLIMVIECTYRKY 297 Query: 299 KDENHIRWAFENLQETLKEF 318 + ++HI+WA+EN E K+ Sbjct: 298 ESKDHIKWAYENFAEGYKQL 317 >A9FXX2.1 A9FXX2_SORC5 Probable Aminoglycoside phosphotransferase Length = 325 Score = 241 bits (615), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 135/310 (43%), Positives = 176/310 (56%), Gaps = 12/310 (3%) Query: 1 MESFTKVKLDDQQLRALAASTFGT------DTAIISSKELTGGFFNAAYDLELSDGRSVI 54 MES TK Q+ A+AA FG + A++ EL G+FNAAY + L+DGR VI Sbjct: 1 MESKTKNIKTRPQIAAMAARAFGGMALADGEGAVV---ELKDGWFNAAYSVRLADGREVI 57 Query: 55 LKVAPAAETETLSYEKDIMRAEVEALRLVQAAGTVPVPAVYGFDESLELIPSPFFFMEKI 114 LK+AP + E + YEK+IM EV A+RLV+ +PVP +Y FD++ EL S +FFMEKI Sbjct: 58 LKIAPPRDAEVMLYEKNIMTTEVAAMRLVRRNPAIPVPQIYHFDDTRELCDSDYFFMEKI 117 Query: 115 EGTPYNEVKGSLPDEVRASIERELGRYQRLINGIKGERFGLFGQPQDTAARTWRETFTAM 174 V+ +LP E +ASI+ +G R ING G FG G P D A TWRE FT + Sbjct: 118 AADNLEHVRANLPPETQASIDLHIGEIVREINGFTGTYFGYDGNP-DLRASTWREAFTRI 176 Query: 175 IHNVLDDGQRLGAVLPASYDEIKQGLEKFLPALEEVTEPRLIHWDLWNGNIFVKDGVIVS 234 + +VL+DG R G YDEI+ + + PALEEVT P L+HWD+WN N+FVK+G + Sbjct: 177 MESVLEDGARKGVAYDQGYDEIRAAVLRHAPALEEVTTPCLVHWDVWNPNVFVKEGRVAG 236 Query: 235 IIDWERALWGDVLMEYYFRHF--ENSPAFFEGYGAAFDSPNERXXXXXXXXXXXXXXVIE 292 IID+ERALW D LME FR E + GYG + E E Sbjct: 237 IIDFERALWADPLMEAQFRLLSAEGITSCMRGYGKTSFTFEEERRCRLYSLHLALVMNTE 296 Query: 293 CYSRQYKDEN 302 CY R Y ++ Sbjct: 297 CYYRNYDTDS 306 >A9AV34.1 A9AV34_HERA2 Aminoglycoside phosphotransferase Length = 325 Score = 231 bits (589), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 130/310 (41%), Positives = 173/310 (55%), Gaps = 7/310 (2%) Query: 1 MESFTKVKLDDQQLRALAASTF-GTDTAIISS--KELTGGFFNAAYDLELSDGRSVILKV 57 M S TK +Q+ + A F G A +EL G+FNAAY + L+DGR V+LK+ Sbjct: 1 MYSTTKNAKTREQIATMVAHAFEGMQLAPHEQAIQELKDGWFNAAYAISLADGREVVLKI 60 Query: 58 APAAETETLSYEKDIMRAEVEALRLVQAAGTVPVPAVYGFDESLELIPSPFFFMEKIEGT 117 AP + + LSYE++IM+ EV +RLVQ +PVP +Y FDESL + SP+FFM+KI G Sbjct: 61 APLPDADVLSYERNIMQTEVATMRLVQQNPAIPVPTIYFFDESLSMCDSPYFFMQKIAGD 120 Query: 118 PYNEVKGSLPDEVRASIERELGRYQRLINGIKGERFGLFGQPQDTAARTWRETFTAMIHN 177 V+ +L V+A +++ +G IN G FG G P+ +W+ TF +I Sbjct: 121 NLEHVRAALEPAVQAKVDQHIGAIIHEINQFSGSYFGYEGNPK-LRGDSWKATFITLIDA 179 Query: 178 VLDDGQRLGAVLPASYDEIKQGLEKFLPALEEVTEPRLIHWDLWNGNIFVKDGVIVSIID 237 +L DGQR L SY EI+ + K AL+EVT P L+HWD WN N FV+ IV IID Sbjct: 180 LLADGQRKNVELEYSYAEIRAAVLKHAAALDEVTTPYLVHWDAWNANFFVEHDKIVGIID 239 Query: 238 WERALWGDVLMEYYFRHF--ENSPAFFEGYGAAFDSPNERXXXXXXXXXXXXXXVIECYS 295 +ERALWGD LME FR F E+ F +GYG +P E IECY Sbjct: 240 FERALWGDPLMEAQFRPFSGESLSNFMQGYGKIAFTPAEEQRNQLYSLYLALVMAIECYY 299 Query: 296 RQYKDENHIR 305 R Y D + +R Sbjct: 300 RNY-DTDFVR 308 >B9LIA9.1 B9LIA9_CHLSY Aminoglycoside phosphotransferase Length = 329 Score = 207 bits (526), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 119/304 (39%), Positives = 163/304 (53%), Gaps = 8/304 (2%) Query: 1 MESFTKVKLDDQQLRALAASTFGTDTAIISSK----ELTGGFFNAAYDLELSDGRSVILK 56 M+S TK Q+ A+ A FG A+ + EL G+FNA Y + L++G+ VILK Sbjct: 1 MKSKTKNTKTRAQIEAMVARAFG-GMALADDEGAVYELKDGWFNAVYSIRLANGQEVILK 59 Query: 57 VAPAAETETLSYEKDIMRAEVEALRLVQAAGTVPVPAVYGFDESLELIPSPFFFMEKIEG 116 +AP + E +SYEK+IM EV +RLV+ +PVP +Y D++ E+ + +FFMEK+ G Sbjct: 60 IAPPPDAEVMSYEKNIMATEVATMRLVRQNPAIPVPNIYFCDDTHEVCDADYFFMEKLAG 119 Query: 117 TPYNEVKGSLPDEVRASIERELGRYQRLINGIKGERFGLFGQPQDTAARTWRETFTAMIH 176 ++ LP A++E ++G R IN +G FG G P D A TW+E F ++ Sbjct: 120 DTLAHMRARLPAATLATLEYQIGTIIREINSFRGTYFGYEGNP-DLRASTWKEAFIRIMD 178 Query: 177 NVLDDGQRLGAVLPASYDEIKQGLEKFLPALEEVTEPRLIHWDLWNGNIFVKDGVIVSII 236 VL+D R SYDEI+ + K ALEEVT P L+HWD W+ N FV+ G IV II Sbjct: 179 AVLEDAARKRVTFDYSYDEIRDVVWKHAAALEEVTIPCLVHWDAWDANFFVQQGRIVGII 238 Query: 237 DWERALWGDVLMEYYFRHF--ENSPAFFEGYGAAFDSPNERXXXXXXXXXXXXXXVIECY 294 D+ER LW D LME FR F + GYG + E+ ECY Sbjct: 239 DFERVLWADPLMEAQFRPFFGDGITNSMRGYGKTSFTFAEKQRCYLYSLHLALVMHTECY 298 Query: 295 SRQY 298 R Y Sbjct: 299 YRNY 302 >A9WDM2.1 A9WDM2_CHLAA Aminoglycoside phosphotransferase Length = 329 Score = 207 bits (526), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 119/304 (39%), Positives = 163/304 (53%), Gaps = 8/304 (2%) Query: 1 MESFTKVKLDDQQLRALAASTFGTDTAIISSK----ELTGGFFNAAYDLELSDGRSVILK 56 M+S TK Q+ A+ A FG A+ + EL G+FNA Y + L++G+ VILK Sbjct: 1 MKSKTKNTKTRAQIEAMVARAFG-GMALADDEGAVYELKDGWFNAVYSIRLANGQEVILK 59 Query: 57 VAPAAETETLSYEKDIMRAEVEALRLVQAAGTVPVPAVYGFDESLELIPSPFFFMEKIEG 116 +AP + E +SYEK+IM EV +RLV+ +PVP +Y D++ E+ + +FFMEK+ G Sbjct: 60 IAPPPDAEVMSYEKNIMATEVATMRLVRQNPAIPVPNIYFCDDTHEVCDADYFFMEKLAG 119 Query: 117 TPYNEVKGSLPDEVRASIERELGRYQRLINGIKGERFGLFGQPQDTAARTWRETFTAMIH 176 ++ LP A++E ++G R IN +G FG G P D A TW+E F ++ Sbjct: 120 DTLAHMRARLPAATLATLEYQIGTIIREINSFRGTYFGYEGNP-DLRASTWKEAFIRIMD 178 Query: 177 NVLDDGQRLGAVLPASYDEIKQGLEKFLPALEEVTEPRLIHWDLWNGNIFVKDGVIVSII 236 VL+D R SYDEI+ + K ALEEVT P L+HWD W+ N FV+ G IV II Sbjct: 179 AVLEDAARKRVTFDYSYDEIRDVVWKHAAALEEVTIPCLVHWDAWDANFFVQQGRIVGII 238 Query: 237 DWERALWGDVLMEYYFRHF--ENSPAFFEGYGAAFDSPNERXXXXXXXXXXXXXXVIECY 294 D+ER LW D LME FR F + GYG + E+ ECY Sbjct: 239 DFERVLWADPLMEAQFRPFFGDGITNSMRGYGKTSFTFAEKQRCYLYSLHLALVMHTECY 298 Query: 295 SRQY 298 R Y Sbjct: 299 YRNY 302 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,935,351,412 Number of extensions: 81377032 Number of successful extensions: 196271 Number of sequences better than 1.0e-05: 54 Number of HSP's gapped: 196269 Number of HSP's successfully gapped: 54 Length of query: 326 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 190 Effective length of database: 1,779,438,163 Effective search space: 338093250970 Effective search space used: 338093250970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)