BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0016.p (310 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65D31.1 Q65D31_BACLD YxiA 407 e-112 B3FRL6.1 B3FRL6_BACSU Endo-alpha-1,5-L-arabinanase EC=3.2.1.99 395 e-108 P42293.2 YXIA_BACSU Uncharacterized protein yxiA 395 e-108 A3DBC8.1 A3DBC8_CLOTH Alpha-L-arabinofuranosidase B 380 e-104 Q9KBP7.1 Q9KBP7_BACHD BH1878 protein 377 e-103 >Q65D31.1 Q65D31_BACLD YxiA Length = 475 Score = 407 bits (1045), Expect = e-112, Method: Compositional matrix adjust. Identities = 195/306 (63%), Positives = 232/306 (75%), Gaps = 8/306 (2%) Query: 3 PNVVDPDVFFDKDGKLWMVYGSYSGGIFILGLDPRTGFPLPDQGYGKKLLGGNHARIEGP 62 PNVVDP FFD++GKLWMVYGSYSGGIFIL +D +TGFPLP QGYGKKL+GGNH+RIEG Sbjct: 171 PNVVDPHTFFDQNGKLWMVYGSYSGGIFILEMDKKTGFPLPGQGYGKKLIGGNHSRIEGA 230 Query: 63 YMLYSPETXXXXXXXXXXXXDANGGYNIRVARSRNPDGPFEDSEGKAMIDAQGTPGKLFD 122 Y+LY PET A+GGYNIRVARS+NPDGP+ D+EG AMID +G G LFD Sbjct: 231 YILYHPETQYYYLYMSFGGLAADGGYNIRVARSKNPDGPYYDAEGHAMIDVRGKEGTLFD 290 Query: 123 DPAYSPYGMKLMGSFQFMNTEDDPEASGTGYISPGHNSAYYDDKSGQYYLIFHTRFPGRG 182 D + PYG+KLMG+F F N GY+SPGHNSA+YD+KSG+ YLIFHTRFPGRG Sbjct: 291 DRSIEPYGVKLMGNFSFNNK--------NGYVSPGHNSAFYDEKSGKSYLIFHTRFPGRG 342 Query: 183 EEHEVRVHQMFMNKEGWPVVAPHRYSGEKIGKYTAKEISGGYAFINHGKDITADIAGSKP 242 EEHEVRVHQ+ MNK+GWPVVAPHRY+GEK+ K ++ G Y + HGKDI+ADI SK Sbjct: 343 EEHEVRVHQLLMNKQGWPVVAPHRYAGEKLEKVKKSDVIGDYELVRHGKDISADIKESKE 402 Query: 243 IELTADGKITGAVAGTWKLAGDHTAELTINGVVYSGVFLREWNEAAGSQVMTFTAASAEG 302 I L +GKITGAVAGTWK G + EL I+G Y GVFLR+W+ A+ +VMTF+A S EG Sbjct: 403 IRLNQNGKITGAVAGTWKNTGHNKIELKIDGKTYDGVFLRQWDAASERKVMTFSALSREG 462 Query: 303 VSLWGS 308 ++WGS Sbjct: 463 DAVWGS 468 >B3FRL6.1 B3FRL6_BACSU Endo-alpha-1,5-L-arabinanase EC=3.2.1.99 Length = 469 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 185/307 (60%), Positives = 233/307 (75%), Gaps = 4/307 (1%) Query: 3 PNVVDPDVFFDKDGKLWMVYGSYSGGIFILGLDPRTGFPLPDQGYGKKLLGGNHARIEGP 62 PNVVDP FFDKDGKLWMVYGSYSGGIFIL ++P+TGFPLP QGYGKKLLGGNH+RIEGP Sbjct: 167 PNVVDPHTFFDKDGKLWMVYGSYSGGIFILEMNPKTGFPLPGQGYGKKLLGGNHSRIEGP 226 Query: 63 YMLYSPETXXXXXXXXXXXXDANGGYNIRVARSRNPDGPFEDSEGKAMIDAQGTPGKLFD 122 Y+LY+P+T DA GGYNIRVARS+ PDGP+ D+EG M+D +G G FD Sbjct: 227 YVLYNPDTQYYYLYLSYGGLDATGGYNIRVARSKKPDGPYYDAEGNPMLDVRGKGGTFFD 286 Query: 123 DPAYSPYGMKLMGSFQFMNTEDDPEASGTGYISPGHNSAYYDDKSGQYYLIFHTRFPGRG 182 D + PYG+KLMGS+ F TE++ GTGY+SPGHNSAYYD+K+G+ YLIFHTRFPGRG Sbjct: 287 DRSIEPYGVKLMGSYTF-ETENE---KGTGYVSPGHNSAYYDEKTGRSYLIFHTRFPGRG 342 Query: 183 EEHEVRVHQMFMNKEGWPVVAPHRYSGEKIGKYTAKEISGGYAFINHGKDITADIAGSKP 242 EEHEVRVHQ+FMNK+GWPV AP+RY+GE + + K+I+G Y I HGKDI+ADI + Sbjct: 343 EEHEVRVHQLFMNKDGWPVAAPYRYAGETLKEVKQKDITGTYKLIQHGKDISADIKQTIN 402 Query: 243 IELTADGKITGAVAGTWKLAGDHTAELTINGVVYSGVFLREWNEAAGSQVMTFTAASAEG 302 I+L + I+G + GTW+ G +TA++T+ G Y+GVFLR+W+ VMTF+ + G Sbjct: 403 IQLNKNHTISGEMTGTWRKTGKNTADITLAGKKYNGVFLRQWDSVREKNVMTFSVLNTSG 462 Query: 303 VSLWGSQ 309 ++WGS+ Sbjct: 463 EAVWGSK 469 >P42293.2 YXIA_BACSU Uncharacterized protein yxiA Length = 469 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 185/307 (60%), Positives = 233/307 (75%), Gaps = 4/307 (1%) Query: 3 PNVVDPDVFFDKDGKLWMVYGSYSGGIFILGLDPRTGFPLPDQGYGKKLLGGNHARIEGP 62 PNVVDP FFDKDGKLWMVYGSYSGGIFIL ++P+TGFPLP QGYGKKLLGGNH+RIEGP Sbjct: 167 PNVVDPHTFFDKDGKLWMVYGSYSGGIFILEMNPKTGFPLPGQGYGKKLLGGNHSRIEGP 226 Query: 63 YMLYSPETXXXXXXXXXXXXDANGGYNIRVARSRNPDGPFEDSEGKAMIDAQGTPGKLFD 122 Y+LY+P+T DA GGYNIRVARS+ PDGP+ D+EG M+D +G G FD Sbjct: 227 YVLYNPDTQYYYLYLSYGGLDATGGYNIRVARSKKPDGPYYDAEGNPMLDVRGKGGTFFD 286 Query: 123 DPAYSPYGMKLMGSFQFMNTEDDPEASGTGYISPGHNSAYYDDKSGQYYLIFHTRFPGRG 182 D + PYG+KLMGS+ F TE++ GTGY+SPGHNSAYYD+K+G+ YLIFHTRFPGRG Sbjct: 287 DRSIEPYGVKLMGSYTF-ETENE---KGTGYVSPGHNSAYYDEKTGRSYLIFHTRFPGRG 342 Query: 183 EEHEVRVHQMFMNKEGWPVVAPHRYSGEKIGKYTAKEISGGYAFINHGKDITADIAGSKP 242 EEHEVRVHQ+FMNK+GWPV AP+RY+GE + + K+I+G Y I HGKDI+ADI + Sbjct: 343 EEHEVRVHQLFMNKDGWPVAAPYRYAGETLKEVKQKDITGTYKLIQHGKDISADIKQTIN 402 Query: 243 IELTADGKITGAVAGTWKLAGDHTAELTINGVVYSGVFLREWNEAAGSQVMTFTAASAEG 302 I+L + I+G + GTW+ G +TA++T+ G Y+GVFLR+W+ VMTF+ + G Sbjct: 403 IQLNKNHTISGEMTGTWRKTGKNTADITLAGKKYNGVFLRQWDSVREKNVMTFSVLNTSG 462 Query: 303 VSLWGSQ 309 ++WGS+ Sbjct: 463 EAVWGSK 469 >A3DBC8.1 A3DBC8_CLOTH Alpha-L-arabinofuranosidase B Length = 707 Score = 380 bits (976), Expect = e-104, Method: Compositional matrix adjust. Identities = 178/311 (57%), Positives = 233/311 (74%), Gaps = 9/311 (2%) Query: 1 VKPNVVDPDVFFDKDGKLWMVYGSYSGGIFILGLDPRTGFPLPDQGYGKKLLGGNHARIE 60 + PN VDP FFDK+G LWMVYGSYSGGI+IL ++P TG P QGYGKKLLG NH+RIE Sbjct: 403 IHPNAVDPHTFFDKNGDLWMVYGSYSGGIYILKMNPSTGKQYPGQGYGKKLLGANHSRIE 462 Query: 61 GPYMLYSPETXXXXXXXXXXXXDANGGYNIRVARSRNPDGPFEDSEGKAMIDAQGTPGKL 120 GPY+ YSP+T A+GGYNIRVARS+NPDGP+ D+EG MI+ +G G Sbjct: 463 GPYIQYSPDTGYYYLFLSFGGLTADGGYNIRVARSQNPDGPYYDAEGNNMINCRGPAGSF 522 Query: 121 FDDPAYSPYGMKLMGSFQFMNTEDDPEASGTGYISPGHNSAYYDDKSGQYYLIFHTRFPG 180 FDD A PYG+KLMG+F+F++ SG GY+SPGHNS YYD++SG+Y++IFHTRFPG Sbjct: 523 FDDRAIEPYGVKLMGNFRFVD-------SGIGYVSPGHNSTYYDEESGKYFIIFHTRFPG 575 Query: 181 RGEEHEVRVHQMFMNKEGWPVVAPHRYSGEKIGKYTAKEISGGYAFINHGKDITADIAGS 240 +GE H+VRVHQMF+N++GWPV+APHRY+GE I +Y+ +E++G YA+INHG+DI+A+I S Sbjct: 576 KGEMHQVRVHQMFINEDGWPVIAPHRYAGETIRRYSPEEVAGTYAYINHGRDISANIKNS 635 Query: 241 KPIELTADGKITG-AVAGTWKLAGDHTAELTINGVVYSGVFLREWNEAAGSQVMTFTAAS 299 I L DG ++G V G W+L+G+H LT+NG Y+GV L++W++ VMTF+A S Sbjct: 636 VRISLNKDGTVSGNGVNGRWELSGEHYIRLTVNGTNYTGVVLQQWDDGLKKYVMTFSAIS 695 Query: 300 AEG-VSLWGSQ 309 G V++WGSQ Sbjct: 696 RNGNVTIWGSQ 706 >Q9KBP7.1 Q9KBP7_BACHD BH1878 protein Length = 781 Score = 377 bits (968), Expect = e-103, Method: Compositional matrix adjust. Identities = 177/309 (57%), Positives = 222/309 (71%), Gaps = 3/309 (0%) Query: 1 VKPNVVDPDVFFDKDGKLWMVYGSYSGGIFILGLDPRTGFPLPDQGYGKKLLGGNHARIE 60 V PN +DP VFFD++GKLWMVYGSYSGGIFIL +DP TGFPLP QGYG L+GGNH+RIE Sbjct: 172 VHPNAIDPHVFFDEEGKLWMVYGSYSGGIFILEMDPDTGFPLPGQGYGTHLMGGNHSRIE 231 Query: 61 GPYMLYSPETXXXXXXXXXXXXDANGGYNIRVARSRNPDGPFEDSEGKAMIDAQGTPGKL 120 PY+ Y ET D+ GGYN+RVARS+ PDGP+ D EG M + +G PG L Sbjct: 232 APYIHYDKETEYYYLYVTFGGLDSTGGYNMRVARSKQPDGPYVDGEGHLMSEVRGAPGTL 291 Query: 121 FDDPAYSPYGMKLMGSFQFMNTEDDPEASGTGYISPGHNSAYYDDKSGQYYLIFHTRFPG 180 FDD + PYG+K MG+F F + + GTGY+SPGHNS YYDD+ + +L FHTRFP Sbjct: 292 FDDASIEPYGVKQMGNFLF---KRELGERGTGYVSPGHNSVYYDDEVDKIFLFFHTRFPE 348 Query: 181 RGEEHEVRVHQMFMNKEGWPVVAPHRYSGEKIGKYTAKEISGGYAFINHGKDITADIAGS 240 RGE HE+RVHQMFMN++GWPV+AP+RY+GE + K AKE+ G Y +INHGK+I+A+I S Sbjct: 349 RGEAHEIRVHQMFMNRKGWPVIAPYRYTGESLEKVKAKEVIGDYQWINHGKEISAEIKES 408 Query: 241 KPIELTADGKITGAVAGTWKLAGDHTAELTINGVVYSGVFLREWNEAAGSQVMTFTAASA 300 + L + K+ G V G WK GD+ EL I+G Y GVFLR+W+ A +VMTFTA S Sbjct: 409 VTVRLEKNHKVLGDVTGKWKKVGDYHVELQIDGETYYGVFLRQWDPVAEREVMTFTALSE 468 Query: 301 EGVSLWGSQ 309 EG+++WGSQ Sbjct: 469 EGIAIWGSQ 477 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.137 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,140,553,999 Number of extensions: 98943356 Number of successful extensions: 135041 Number of sequences better than 1.0e-05: 88 Number of HSP's gapped: 134835 Number of HSP's successfully gapped: 88 Length of query: 310 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 175 Effective length of database: 1,788,859,178 Effective search space: 313050356150 Effective search space used: 313050356150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)