BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0011.p (386 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZJV7.1 C0ZJV7_BREBN Citrate synthase EC=2.3.3.1 441 e-122 P39119.2 CISY_BACSU Citrate synthase 1 EC=2.3.3.1 416 e-114 A7Z2V8.1 A7Z2V8_BACA2 CitA 407 e-112 Q65LZ6.1 Q65LZ6_BACLD Citrate synthase I 391 e-107 Q2B4H5.1 Q2B4H5_9BACI CitA 385 e-105 >C0ZJV7.1 C0ZJV7_BREBN Citrate synthase EC=2.3.3.1 Length = 367 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 207/358 (57%), Positives = 264/358 (73%), Gaps = 1/358 (0%) Query: 20 GLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQLK 79 GL+G+ ETD+ LVDG G LVYRG+WA++LA+ +EEVA LLW G+LPS +EL LK Sbjct: 10 GLDGIAVAETDLSLVDGINGLLVYRGHWARDLAIHHTFEEVAHLLWFGHLPSEQELTDLK 69 Query: 80 NEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQGEAVRLAAILP 139 +++ A+R+LPE+++ I++L+PA V MM VL++ V+ALG + +PP E + LAA +P Sbjct: 70 DKLKANRELPEHVKGILKLIPADVDMMSVLRTGVSALGSSSHG-FPPTIDEVIALAAKMP 128 Query: 140 TIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGMNAS 199 TI+AYR+ H ANYLYML G KP AHV+AL AY IL EHGMNAS Sbjct: 129 TIVAYRFACLEGLEPLAPNPELDHTANYLYMLKGVKPNAAHVRALDAYLILTQEHGMNAS 188 Query: 200 TFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNALEN 259 TF+ RVV STESD+ +S+ AIG +KGPLHGGAP EV M+EAIGTKE AEPWLRN LE+ Sbjct: 189 TFSGRVVSSTESDLISSITAAIGTLKGPLHGGAPSEVTEMIEAIGTKENAEPWLRNKLES 248 Query: 260 GNKLMGFGHRIYKTKDPRAEALQIATQEMIGTDPSFDLALHVEAAAIRLLEEYKPGRRLF 319 G +LMGFGHR+YKT DPRA AL++ E+ DP FDLA HVE ++LLEEYKPGR+L Sbjct: 249 GGRLMGFGHRVYKTIDPRATALRVVASELSSNDPWFDLATHVEKVGLQLLEEYKPGRKLN 308 Query: 320 TNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYTGPMP 377 TNVEF+AAA+++A+ L ++FTPTF R++GW+AHI+EQA NNRI RPQS Y G MP Sbjct: 309 TNVEFFAAAVMRAVGLDDNLFTPTFAVSRVVGWSAHILEQAGNNRIIRPQSNYIGTMP 366 >P39119.2 CISY_BACSU Citrate synthase 1 EC=2.3.3.1 Length = 366 Score = 416 bits (1069), Expect = e-114, Method: Compositional matrix adjust. Identities = 200/358 (55%), Positives = 257/358 (71%), Gaps = 1/358 (0%) Query: 20 GLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQLK 79 GL+G+ ET I +DGE+G L+YRG+ AK++A+ +EE A+L+ G LPS EEL+ K Sbjct: 5 GLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEELQVFK 64 Query: 80 NEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQGEAVRLAAILP 139 +++AA R LPE+I R+I+ LP ++ M VL++ V+ALGE + P EA+RL AI P Sbjct: 65 DKLAAERNLPEHIERLIQSLPNNMDDMSVLRTVVSALGEN-TYTFHPKTEEAIRLIAITP 123 Query: 140 TIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGMNAS 199 +IIAYR R H NY YMLTGE+P+EA KAL Y IL EHGMNAS Sbjct: 124 SIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQPSEAKKKALETYMILATEHGMNAS 183 Query: 200 TFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNALEN 259 TF+ARV LSTESD+ ++V A+G MKGPLHGGAP V MLE IG KE AE +L+ LE Sbjct: 184 TFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEKLEK 243 Query: 260 GNKLMGFGHRIYKTKDPRAEALQIATQEMIGTDPSFDLALHVEAAAIRLLEEYKPGRRLF 319 G +LMGFGHR+YKTKDPRAEAL+ +E+ G D DLALHVEA AIRLLE YKPGR+L+ Sbjct: 244 GERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGRKLY 303 Query: 320 TNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYTGPMP 377 TNVEFYAAA+++A++ ++FTPTF+A R++GW AH++EQA NN IFRP + YTG +P Sbjct: 304 TNVEFYAAAVMRAIDFDDELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAIP 361 >A7Z2V8.1 A7Z2V8_BACA2 CitA Length = 369 Score = 407 bits (1046), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/358 (53%), Positives = 255/358 (71%), Gaps = 1/358 (0%) Query: 20 GLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQLK 79 GL+G+ ET I +DGE+G+L+YRGY AK++A+ +EE A+L+ NG LP +L + Sbjct: 7 GLKGITCAETAISHIDGEKGHLIYRGYDAKDIALQSSFEEAAYLILNGALPDERQLLAFQ 66 Query: 80 NEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQGEAVRLAAILP 139 E+AA R LP+++ ++E LP + M VL++ V+ LG + +PP EA++L A+ P Sbjct: 67 EELAAERGLPKHLIDLLETLPDEMDDMAVLRTAVSGLGTD-SFTYPPKPEEAIKLIAVTP 125 Query: 140 TIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGMNAS 199 TIIAYRYR H NYLYMLTG +P+ A KAL Y IL MEHGMNAS Sbjct: 126 TIIAYRYRRKQGQEALSPEARYGHVENYLYMLTGRQPSAAQKKALETYMILAMEHGMNAS 185 Query: 200 TFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNALEN 259 TF+ARV +STESD+ ++V A+G MKGPLHGGAP V MLE IG KE AE +L L Sbjct: 186 TFSARVTVSTESDLVSAVTAALGTMKGPLHGGAPSGVTKMLEDIGKKENAEAYLAAKLMR 245 Query: 260 GNKLMGFGHRIYKTKDPRAEALQIATQEMIGTDPSFDLALHVEAAAIRLLEEYKPGRRLF 319 G +LMGFGHR+YKTKDPRAEAL+I +E+ G DPS DLAL+VE AIRLLE+YKPGR+L+ Sbjct: 246 GERLMGFGHRVYKTKDPRAEALRIKAEEVAGNDPSLDLALYVEKEAIRLLEQYKPGRKLY 305 Query: 320 TNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYTGPMP 377 TNVEFYAAA+++A+++ D+FTPTF+A R+ GW AH++EQ+A+N I+RP + YTG P Sbjct: 306 TNVEFYAAAVMQAIDVDNDLFTPTFSASRMAGWCAHVLEQSADNMIYRPSAKYTGAFP 363 >Q65LZ6.1 Q65LZ6_BACLD Citrate synthase I Length = 363 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 188/357 (52%), Positives = 248/357 (69%), Gaps = 1/357 (0%) Query: 20 GLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQLK 79 GL+G+ ET I +DGE+G L+YRGY A ELA++ +EEVA+L+ NG P+ EL + Sbjct: 5 GLKGITCVETAISRIDGEKGKLIYRGYNADELALSSTFEEVAYLILNGAFPTEAELSRFN 64 Query: 80 NEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQGEAVRLAAILP 139 +A+ R LPE R+I LPA + M VL++ V++ G+ + A+ P +A+RL A +P Sbjct: 65 ELLASFRVLPEACERLIRSLPAELDDMAVLRTAVSSFGDI-SFAFKPTMEQALRLIAAVP 123 Query: 140 TIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGMNAS 199 +IIA+R R NY YML G++PTEA KAL Y IL MEHGMNAS Sbjct: 124 SIIAFRKRLADGEEPVQPLDELGFVENYFYMLKGKRPTEAQKKALETYMILAMEHGMNAS 183 Query: 200 TFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNALEN 259 TF+ARV +STESD+ ++V A+G MKGPLHGGAP V MLE IG K+ AE ++R LE Sbjct: 184 TFSARVTVSTESDLVSAVTSALGTMKGPLHGGAPSAVTDMLEGIGEKQHAESYIREKLEK 243 Query: 260 GNKLMGFGHRIYKTKDPRAEALQIATQEMIGTDPSFDLALHVEAAAIRLLEEYKPGRRLF 319 G +LMGFGHR+YKT DPRA+AL++ +E+ G D DLALHVE AIRLLEEYKPGR+L+ Sbjct: 244 GERLMGFGHRVYKTHDPRAKALRVKAEEIAGGDRDIDLALHVENTAIRLLEEYKPGRKLY 303 Query: 320 TNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYTGPM 376 TNVEFYAAA++KA++ +FTPTF+A R++GW AH++EQA NN IFRP + Y G + Sbjct: 304 TNVEFYAAAVMKAIDFDAALFTPTFSAARMVGWCAHVLEQADNNMIFRPSAKYVGEL 360 >Q2B4H5.1 Q2B4H5_9BACI CitA Length = 369 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 194/371 (52%), Positives = 253/371 (68%), Gaps = 3/371 (0%) Query: 6 MESEKELKIMAKVTGLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLW 65 M+++KE ++ GL+G++A ET +G VDG +G L+YRGY EL +EE AFLLW Sbjct: 1 MKADKERRMRMIAKGLKGILAAETSVGHVDGLKGQLIYRGYEIGELTRKCSFEEAAFLLW 60 Query: 66 NGYLPSAEELEQLKNEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWP 125 +G LP+ EL+ LK E+ + R +P ++ II +P S MM VL++ V++LG + Sbjct: 61 HGRLPARGELDDLKTELHSFRAIPPHVASIITAMPVSFSMMDVLRTAVSSLG---GSLEK 117 Query: 126 PAQGEAVRLAAILPTIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALS 185 PA +AV+L A++P +IA+R R HAANYLYML G+ P +AH +AL Sbjct: 118 PAVRQAVQLTALIPVMIAFRSRLLEGKQPVDPEPSLGHAANYLYMLKGQIPEKAHSEALE 177 Query: 186 AYQILCMEHGMNASTFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGT 245 Y +L MEHGMNASTF+ARV STESD+ +++ AIG MKGPLHGGAP VI +L+ I Sbjct: 178 TYLLLTMEHGMNASTFSARVTASTESDLISAITSAIGTMKGPLHGGAPSGVIELLDEIME 237 Query: 246 KERAEPWLRNALENGNKLMGFGHRIYKTKDPRAEALQIATQEMIGTDPSFDLALHVEAAA 305 + AE +R LE G KLMGFGHRIYKT DPRA AL+ Q+ G D D+AL E A Sbjct: 238 RGDAEAVIREKLERGEKLMGFGHRIYKTLDPRAAALKDKLQQFAGHDTWLDMALVTEQTA 297 Query: 306 IRLLEEYKPGRRLFTNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRI 365 +RLL EYKPGR L+TNVE+YAAAI+KAL++ +FTPTFTA RI GWTAH+IEQA NN I Sbjct: 298 LRLLGEYKPGRSLYTNVEYYAAAIMKALKMDSSLFTPTFTAARIAGWTAHVIEQADNNAI 357 Query: 366 FRPQSIYTGPM 376 FRP+S+Y GP+ Sbjct: 358 FRPESLYIGPL 368 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,129,208,067 Number of extensions: 83848668 Number of successful extensions: 221572 Number of sequences better than 1.0e-05: 3673 Number of HSP's gapped: 212397 Number of HSP's successfully gapped: 3676 Length of query: 386 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 249 Effective length of database: 1,770,017,148 Effective search space: 440734269852 Effective search space used: 440734269852 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)