BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0006.p (349 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K364.1 B3K364_9BACI VanZ family protein 293 2e-77 C3DFD0.1 C3DFD0_BACTS VanZ 211 9e-53 C3A1G3.1 C3A1G3_BACMY VanZ 211 1e-52 B7HWA2.1 B7HWA2_BACC7 Putative membrane protein 207 2e-51 C2RZ46.1 C2RZ46_BACCE VanZ 207 2e-51 >B3K364.1 B3K364_9BACI VanZ family protein Length = 368 Score = 293 bits (749), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 148/297 (49%), Positives = 202/297 (68%), Gaps = 1/297 (0%) Query: 1 MFQSYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLI 60 M ++YLFPIS AF++FP+AAL+ +PFLVVQYR++GY NK R + NA YL+ Sbjct: 1 MLEAYLFPISYAFLTFPIAALLCAVPFLVVQYRKYGYFNKFRGFLLYLFLLYMMNAVYLV 60 Query: 61 MLPMPASRHNAA-PVGSIIQHVPLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNV 119 +LP+PASRHNA V S++Q +PL FIQ+I + + + G +Y + ++ FL FNV Sbjct: 61 LLPLPASRHNAPMDVASVMQWMPLNFIQDILKETKVVWGVPSTYTHLLKERAFLQVVFNV 120 Query: 120 VLTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNT 179 +L VPFG+FL YY R WV C+ SFGLSL FEVTQ+TGIYGF+DHPYR+FDVDDL+ NT Sbjct: 121 LLVVPFGMFLRYYARASWVACLTASFGLSLFFEVTQVTGIYGFYDHPYRLFDVDDLLMNT 180 Query: 180 LGGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTLHSW 239 LGG++G +A W+ LPR+++LD KVD S KRV+YTRR IA+ +D + + L Sbjct: 181 LGGMMGLVLADWMKNHLPRLDRLDEKVDLSQKRVSYTRRAIAFGIDAAVILPIIGLLTVI 240 Query: 240 GMQGAYWITTAVYFVLLPCCTGGRTFGKWVVRISISGQDGRSRLWGLIVRYVLQYGV 296 ++GAY+ +Y+++LP G+T GKWVVR+ I G +L +IVR L Y V Sbjct: 241 NVRGAYFFAVILYYIVLPYMMQGQTLGKWVVRVQIRGAGEHLKLKEVIVRNGLLYVV 297 >C3DFD0.1 C3DFD0_BACTS VanZ Length = 383 Score = 211 bits (537), Expect = 9e-53, Method: Compositional matrix adjust. Identities = 128/359 (35%), Positives = 204/359 (56%), Gaps = 20/359 (5%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +YLFP+ AF+ FP A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYLFPVKTAFILFPFLAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTFDTCSLQSANTQHMQLSPFYFIQEISSHTSAVLTKPATYFYLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFFLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLD---LPIWMIPFL--- 234 GGV+G+ IA + LP+ +LDS +D K V + RR IA +D L I ++P + Sbjct: 183 GGVIGFIIAPIFTYFLPKTNELDSHIDLETKPVGFIRRLIAMQIDWIFLSI-VVPVIKNK 241 Query: 235 -------TLHSW-GMQGAYWITTA--VYFVLLPCCTGGRTFGKWVVRISISGQDGRSRLW 284 + S+ M +IT + +YF+++P T G+T GK ++RI + G+ R L Sbjct: 242 GNSFFVSNMQSYTNMYELIFITCSILIYFIIIPYFTNGKTIGKALLRIHLKGKSDRITLK 301 Query: 285 GLIVRYVLQYGVLIGANVFXXXXXXXXXXXXXWTGIPYSLVMLLDFMFLIHLIIRVFKR 343 L +RY + Y L G N + + +++ +F+IH+++ VF R Sbjct: 302 ELFIRYGIFYFALGGINYILSSSSMLNHTEPLVLLVTLLFLFIINGLFIIHVLLHVFSR 360 >C3A1G3.1 C3A1G3_BACMY VanZ Length = 384 Score = 211 bits (536), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 122/316 (38%), Positives = 186/316 (58%), Gaps = 18/316 (5%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +YLFP+ AF+ FP+ A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYLFPVKTAFILFPILAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTYDTCSLQPANTQHMQLSPFYFIQEISNHTSAILTKPTTYFLLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFCLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTLHSWG 240 GGV+G+ IA + LP+ +LDS++D K V + RR IA +D I + + G Sbjct: 183 GGVIGFIIAPIFTYFLPKANELDSRIDLETKPVGFVRRLIAMQIDWIFLSIVVPVIKNKG 242 Query: 241 -------------MQGAYWITTA--VYFVLLPCCTGGRTFGKWVVRISISGQDGRSRLWG 285 + +IT + +YF+++P T GRT GK ++RI I GQ R L Sbjct: 243 NSFFISNIQSYTNVYELLFITCSILIYFIIIPYFTNGRTIGKALLRIYIKGQAERITLKE 302 Query: 286 LIVRYVLQYGVLIGAN 301 L +RY + Y +L G N Sbjct: 303 LFIRYGIFYFILGGIN 318 >B7HWA2.1 B7HWA2_BACC7 Putative membrane protein Length = 383 Score = 207 bits (526), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 130/366 (35%), Positives = 202/366 (55%), Gaps = 34/366 (9%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +Y+FPI AF+ FP+ A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYIFPIKTAFILFPILAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTYDTCSLQPANTQHMQLSPFYFIQEISNHTSAILTKPATYFYLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFCLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTLHSWG 240 GGV+G+ IA + LP+ +LDS +D K V + RR IA +D I + + G Sbjct: 183 GGVIGFIIAPIFTYFLPKASELDSHIDLETKPVGFVRRFIAMQIDWIFLSIVVPVIKNKG 242 Query: 241 MQGAYWIT-----TAVYFVLLPCC------------TGGRTFGKWVVRISISGQDGRSRL 283 +++++ T +Y ++ C T G+T GK ++RI + GQ R L Sbjct: 243 --NSFFVSNIQSYTNIYELIFITCSIFIYFIIIPYFTNGKTIGKALLRIYVKGQSDRITL 300 Query: 284 WGLIVRYVLQYGVLIGANVFXXXXXXXXXXXXXWTGIPYSLVMLLDF------MFLIHLI 337 L +RY + Y VL G N P LV++L F +F+IH++ Sbjct: 301 KELFIRYGIFYFVLGGINYILSSSSILNLKE------PLVLVVILLFQLVINGLFIIHVL 354 Query: 338 IRVFKR 343 + VF R Sbjct: 355 LHVFSR 360 >C2RZ46.1 C2RZ46_BACCE VanZ Length = 383 Score = 207 bits (526), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 130/366 (35%), Positives = 202/366 (55%), Gaps = 34/366 (9%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +Y+FPI AF+ FP+ A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYIFPIKTAFILFPILAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTYDTCSLQPANTQHMQLSPFYFIQEISNHTSAILTKPATYFYLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFCLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTLHSWG 240 GGV+G+ IA + LP+ +LDS +D K V + RR IA +D I + + G Sbjct: 183 GGVIGFIIAPIFTYFLPKASELDSHIDLETKPVGFVRRFIAMQIDWIFLSIVVPVIKNKG 242 Query: 241 MQGAYWIT-----TAVYFVLLPCC------------TGGRTFGKWVVRISISGQDGRSRL 283 +++++ T +Y ++ C T G+T GK ++RI + GQ R L Sbjct: 243 --NSFFVSNIQSYTNIYELIFITCSIFIYFIIIPYFTNGKTIGKALLRIYVKGQSDRITL 300 Query: 284 WGLIVRYVLQYGVLIGANVFXXXXXXXXXXXXXWTGIPYSLVMLLDF------MFLIHLI 337 L +RY + Y VL G N P LV++L F +F+IH++ Sbjct: 301 KELFIRYGIFYFVLGGINYILSSSSILNLKE------PLVLVVILLFQLVINGLFIIHVL 354 Query: 338 IRVFKR 343 + VF R Sbjct: 355 LHVFSR 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.333 0.147 0.481 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,963,489,743 Number of extensions: 78738058 Number of successful extensions: 284861 Number of sequences better than 1.0e-05: 123 Number of HSP's gapped: 284795 Number of HSP's successfully gapped: 123 Length of query: 349 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 213 Effective length of database: 1,779,438,163 Effective search space: 379020328719 Effective search space used: 379020328719 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 132 (55.5 bits)