BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0002.p (447 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3DFB1.1 A3DFB1_CLOTH Sensor protein EC=2.7.13.3 137 2e-30 B4BDD4.1 B4BDD4_CLOTM Sensor protein EC=2.7.13.3 137 2e-30 B3K453.1 B3K453_9BACI Sensor protein EC=2.7.13.3 135 1e-29 Q8ESP0.1 Q8ESP0_OCEIH Sensor protein EC=2.7.13.3 124 2e-26 Q18AL3.1 Q18AL3_CLOD6 Sensor protein EC=2.7.13.3 115 7e-24 >A3DFB1.1 A3DFB1_CLOTH Sensor protein EC=2.7.13.3 Length = 489 Score = 137 bits (345), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 121/484 (25%), Positives = 211/484 (43%), Gaps = 55/484 (11%) Query: 3 TIRGKMLLSFFLALVVTVGITVALFVRLIDDILVNQVKTQLHGQAEKAEKIL-------- 54 TI+ ++ +A+ V + +T +F I + N +K QL A++ E Sbjct: 5 TIKWRIFKYNIIAISVLLALTTIVFNITIQSYIKNDIKEQLSTIAQRVEDTALYRGPDFF 64 Query: 55 -------------QDSDISRLNNTEFKYVV--------KGMMLNADYLILDANNKIIDAN 93 + + + R NN F++ +LNAD+++LD N II Sbjct: 65 PPQEPQRPRPLPGRQNGMFRENNDLFRFYFFLDRSLREPLSILNADFILLDNNMDIITPP 124 Query: 94 EDQGVGTTLHYALSERQGIAVLHGKKIL-YTKERLSGRPYSIFIYAPLSSLRA------- 145 E + A I L G K+ Y ++ Y I I P+S + Sbjct: 125 EGDFFKPSGEIANEIISKIKKLDGSKVQEYVYFNVNNTDY-ISIVKPVSDKNSFGLGWLI 183 Query: 146 LYGSLMRTTLLAIGFSFFMILAIGL----------LTVSRVVRPLNRLKEAVSRY-EPYR 194 +Y SL + L +G + +I+ + L L ++ P + L + + E Sbjct: 184 IYSSLQKVNQLKLGINMILIVIVLLSALIFAIFSSLAAKKISAPFSSLNQHIRAIAERNF 243 Query: 195 SQDAVFPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAI 254 PVD E+ EL+ + MSE+++ + + Q F QN SHE +TPLMSI + I Sbjct: 244 GVKIEMPVD--AELQELVNSINIMSEKLESYDKAQKTFFQNASHEFRTPLMSIQSYAEGI 301 Query: 255 QDQVVTQETGLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXX 314 + VV + T D+I++++ R+ +V+ LL LSRL+ ++E++ +++D + Sbjct: 302 KYDVVDKNTAADIIIDETKRMTHLVEDLLYLSRLDTIEENYRFADLDFKDFINACQERMY 361 Query: 315 XXXXXRGVALKVE--GESLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGW 372 G+ L+VE E ++ A E +R+ ++ + + + + Sbjct: 362 GIASKSGINLEVENINEPVVVYADEEKLFRAFANIIGNCIRYANNTVSI-VPKIIDRSKI 420 Query: 373 AIHIDDDGNGLSEAEREAVFQRFYTGPNGVTGLGLAICRQIAANLDGELTYSSSPLGGAR 432 I I DDG G+ E +F+RFY G G GLGLAI + I +G++T +S GA Sbjct: 421 EIVIRDDGPGIDSNELPNIFERFYKGKKGNVGLGLAISKNIIERHNGKITARNSE-SGAV 479 Query: 433 FSFI 436 F+ + Sbjct: 480 FTIV 483 >B4BDD4.1 B4BDD4_CLOTM Sensor protein EC=2.7.13.3 Length = 489 Score = 137 bits (345), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 121/484 (25%), Positives = 211/484 (43%), Gaps = 55/484 (11%) Query: 3 TIRGKMLLSFFLALVVTVGITVALFVRLIDDILVNQVKTQLHGQAEKAEKIL-------- 54 TI+ ++ +A+ V + +T +F I + N +K QL A++ E Sbjct: 5 TIKWRIFKYNIIAISVLLALTTIVFNITIQSYIKNDIKEQLSTIAQRVEDTALYRGPDFF 64 Query: 55 -------------QDSDISRLNNTEFKYVV--------KGMMLNADYLILDANNKIIDAN 93 + + + R NN F++ +LNAD+++LD N II Sbjct: 65 PPQEPQRPRPLPGRQNGMFRENNDLFRFYFFLDRSLREPLSILNADFILLDNNMDIITPP 124 Query: 94 EDQGVGTTLHYALSERQGIAVLHGKKIL-YTKERLSGRPYSIFIYAPLSSLRA------- 145 E + A I L G K+ Y ++ Y I I P+S + Sbjct: 125 EGDFFKPSGEIANEIISKIKKLDGSKVQEYVYFNVNNTDY-ISIVKPVSDKNSFGLGWLI 183 Query: 146 LYGSLMRTTLLAIGFSFFMILAIGL----------LTVSRVVRPLNRLKEAVSRY-EPYR 194 +Y SL + L +G + +I+ + L L ++ P + L + + E Sbjct: 184 IYSSLQKVNQLKLGINMILIVIVLLSALIFAIFSSLAAKKISAPFSSLNQHIRAIAERNF 243 Query: 195 SQDAVFPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAI 254 PVD E+ EL+ + MSE+++ + + Q F QN SHE +TPLMSI + I Sbjct: 244 GVKIEMPVD--AELQELVNSINIMSEKLESYDKAQKTFFQNASHEFRTPLMSIQSYAEGI 301 Query: 255 QDQVVTQETGLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXX 314 + VV + T D+I++++ R+ +V+ LL LSRL+ ++E++ +++D + Sbjct: 302 KYDVVDKNTAADIIIDETKRMTHLVEDLLYLSRLDTIEENYRFADLDFKDFINACQERMY 361 Query: 315 XXXXXRGVALKVE--GESLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGW 372 G+ L+VE E ++ A E +R+ ++ + + + + Sbjct: 362 GIASKSGINLEVENINEPVVVYADEEKLFRAFANIIGNCIRYANNTVSI-VPKIIDRSKI 420 Query: 373 AIHIDDDGNGLSEAEREAVFQRFYTGPNGVTGLGLAICRQIAANLDGELTYSSSPLGGAR 432 I I DDG G+ E +F+RFY G G GLGLAI + I +G++T +S GA Sbjct: 421 EIVIRDDGPGIDSNELPNIFERFYKGKKGNVGLGLAISKNIIERHNGKITARNSE-SGAV 479 Query: 433 FSFI 436 F+ + Sbjct: 480 FTIV 483 >B3K453.1 B3K453_9BACI Sensor protein EC=2.7.13.3 Length = 454 Score = 135 bits (339), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 87/309 (28%), Positives = 156/309 (50%), Gaps = 9/309 (2%) Query: 133 SIFIYAPLSSLRALYGSLMRTTLLAIGFSFFMILAIGLLTVSRVVRPLNRLKEAVSRYEP 192 ++ + P+S ++A+ +L+ +L G +++ + L R+++PL L+E + + + Sbjct: 138 TMTLITPVSKIKAIEQALLGRLMLVFGAGAVVMVILSLFITKRLIKPLMHLREELKKVKE 197 Query: 193 YRSQDAVFPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVF 252 + D V V + E+G + + M+ ++++ Q +F QN SHELK+PLMSI G+ Sbjct: 198 RQFSD-VRRVSASGELGAVAESVYEMAGELERYNHVQKQFFQNASHELKSPLMSISGYAE 256 Query: 253 AIQDQVVTQE---TGLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXX 309 I+D + E GLD+IM++S R+ ++V ++ L++L++ D+ + +E++++ Sbjct: 257 GIRDGIFEGEDIRKGLDIIMSESGRMTNIVTEMTLLAKLDSEDDIFKAAEVNMKELVEET 316 Query: 310 XXXXXXXXXXRGVALKV---EGESLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAG 366 +G+ + + + ESL A + RH S I +Q AG Sbjct: 317 IERINPVLVKKGLFVHLSYGDNESLTVYADRDKLLQALLNIVSNGARHASKHIYIQ--AG 374 Query: 367 YPGAGWAIHIDDDGNGLSEAEREAVFQRFYTGPNGVTGLGLAICRQIAANLDGELTYSSS 426 + + DDG+G+SE +F RF G NG TGLGLAI R I G +T + Sbjct: 375 LQKGRVVLTVSDDGDGISEELLPYLFHRFVKGKNGETGLGLAIARAIVERCGGLITAGNR 434 Query: 427 PLGGARFSF 435 GGA S Sbjct: 435 QEGGAVISL 443 >Q8ESP0.1 Q8ESP0_OCEIH Sensor protein EC=2.7.13.3 Length = 460 Score = 124 bits (311), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 83/320 (25%), Positives = 158/320 (49%), Gaps = 10/320 (3%) Query: 119 KILYTKERLSGRPYSIFIYAPLSSLRALYGSLMRTTLLAIGFSFFMILAIGLLTVSRVVR 178 +I+++ E+ + + P+ L+ + + LL + + +++V Sbjct: 126 RIIFSPEQTG---LELILLTPMHDLQVVQQNFFVRLLLVFLVGAVAAVILSYFMTNKLVT 182 Query: 179 PLNRLKEAVSRYEPYRSQDAVFPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSH 238 PL++LK + + E R D + V T EI E+ + M++ ++Q+ ++Q F QN SH Sbjct: 183 PLSQLKTELKKVEN-RQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNASH 241 Query: 239 ELKTPLMSIHGFVFAIQDQVVTQ---ETGLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHW 295 ELKTPLM+I G+ I+D + + E GL+V++ + RL ++++++ L++L+ + Sbjct: 242 ELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAEY 301 Query: 296 PISEIDLRTXXXXXXXXXXXXXXXRGVALKVEGES-LLTVAAGEXXXXXXXXXXXXAVRH 354 ++ G+ L + E+ ++ A E +RH Sbjct: 302 SPFPLEADQFIHQVVDRAHPIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIRH 361 Query: 355 TSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAEREAVFQRFYTGPNGVTGLGLAICRQIA 414 + S + +Q+ I ++DDG+G++E + E +F RF G +G TGLGLAI R I Sbjct: 362 SKSTVNIQVYT--KDHKVMIDVEDDGSGITEEDAEHIFHRFVKGKDGETGLGLAIARAII 419 Query: 415 ANLDGELTYSSSPLGGARFS 434 G ++ S+S +GGA+F+ Sbjct: 420 EQAGGNISVSNSEIGGAKFT 439 >Q18AL3.1 Q18AL3_CLOD6 Sensor protein EC=2.7.13.3 Length = 463 Score = 115 bits (289), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 80/280 (28%), Positives = 133/280 (47%), Gaps = 8/280 (2%) Query: 161 SFFMILAI--GLLTVSRVVRPLNRLKEAVSRYEPYRSQDAVFPVDDTTEIGELITTFQSM 218 F ILAI ++ ++ +P+ L E + + F D E+ EL T Sbjct: 185 CFAGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDK-ELVELSTVMNKS 243 Query: 219 SERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVVTQETGLDVIMNQSLRLIDM 278 +E + ++ Q F QN SHEL+TPLMSI G+ AI+ V+ + D+I+ +S RL DM Sbjct: 244 AEYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSDM 303 Query: 279 VDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXXXXXXXRGVAL--KVEGESLLTVAA 336 V+ LL +S+++ + + + + E DLR +G+ + + E +L Sbjct: 304 VEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLFECD 363 Query: 337 GEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAEREAVFQRFY 396 + A+R+ S+I+ I Y + I+DDG G+ + + VF+RFY Sbjct: 364 EKNISKAFMNLIENALRYAKSEIK--IVCKYNQKNIVVIIEDDGIGIKKEDLPHVFERFY 421 Query: 397 TGPNGVTGLGLAICRQIAANLDGELTYSSSPLGGARFSFI 436 G G G+GL+I + I N G Y + GA+F+ + Sbjct: 422 KGVGGNHGIGLSIVKSI-VNKHGGRIYVENGKKGAKFTIL 460 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,249,161,518 Number of extensions: 86491779 Number of successful extensions: 322272 Number of sequences better than 1.0e-05: 5854 Number of HSP's gapped: 324567 Number of HSP's successfully gapped: 5857 Length of query: 447 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 308 Effective length of database: 1,751,175,118 Effective search space: 539361936344 Effective search space used: 539361936344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)