BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0212.0011.p (602 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL51.1 B3KL51_9BACI GerA spore germination protein 685 0.0 B1DJB3.1 B1DJB3_9BACL GerA spore germination protein 566 e-159 C0GDZ6.1 C0GDZ6_9FIRM GerA spore germination protein 461 e-128 A6TT32.1 A6TT32_ALKMQ GerA spore germination protein 449 e-124 Q0AZ96.1 Q0AZ96_SYNWW GerA spore germination protein 449 e-124 >B3KL51.1 B3KL51_9BACI GerA spore germination protein Length = 544 Score = 685 bits (1767), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/505 (64%), Positives = 394/505 (78%) Query: 61 LQKKRDAERSDSLEQSIIYWQGNDNIPAKLEDTKNTLVEVMGLNSSFDVVFREMTFGGQK 120 L++KR+AE SDS ++S+IYWQ D++PAKL K TL EV+GL SFDVVFREM F G+ Sbjct: 31 LKEKREAETSDSTKESVIYWQQTDDVPAKLSVLKETLKEVIGLGVSFDVVFREMNFAGRN 90 Query: 121 AALLCISGFAKDIIIDEILKRLTYLTPENISADVLASFMGEYIPHIQVEKGDVLSESITK 180 A LL ++GF D+++ EIL RLTYLTP++++ L+ M YIPH+QVE+ LS+ I + Sbjct: 91 AGLLFLNGFTNDVVVTEILTRLTYLTPDDVNNHALSEIMNRYIPHVQVERDTRLSKVIDE 150 Query: 181 VLSGMSVFFLEGETEVIIMDTRSYPSRNPDEPSIERVVRGARDGFTETLLSNIALVRRRV 240 VLSG+ F+E II+DTR YP R +EP++ERVVRGARDGFTETL++N+ LVRRRV Sbjct: 151 VLSGLCALFIEDAHSAIILDTRKYPVRGLEEPAVERVVRGARDGFTETLVTNVNLVRRRV 210 Query: 241 RDPGLKNELHQVGRRTRTDVCVAYIDDIVDKTQVKAVTDKIKNVNIDGIPLADKQLEEAI 300 RDPGL E+ +VGRRTRTDV + +IDD+ D+TQV+A+ KI+ ++++G+PL DKQLEEAI Sbjct: 211 RDPGLHVEIIKVGRRTRTDVSLVFIDDVADRTQVEAIRKKIQEIDLEGLPLGDKQLEEAI 270 Query: 301 VGGGWNPYPMVRYSERPDAVASHLLEGRVVVFVDTSPSVMILPTTFFDLCQHAEENRQTP 360 V GW+PYP+VRYSERPD V+SHLLEGR+V+ VDTSPSVMI+PTTF DLCQHAEENRQTP Sbjct: 271 VNKGWHPYPLVRYSERPDVVSSHLLEGRIVIMVDTSPSVMIMPTTFLDLCQHAEENRQTP 330 Query: 361 FMGTYLRWVRFIGIFASMFLLPLWMLLVIHPELKPPMLDFIGPQKMAKIPLLAQFLIVEL 420 FMGTYLRW+RFIGIFASMFLLPLWMLLV+HPELKPP LD IGPQ+ AKIP++ QFLIVE Sbjct: 331 FMGTYLRWIRFIGIFASMFLLPLWMLLVVHPELKPPGLDIIGPQENAKIPIILQFLIVEF 390 Query: 421 GVDLLRMAAVHTPSPLGSAMGLIAAILVGDIAVKSGFFVNEVVLYMAVASIGMFATPSYE 480 GVDLLR+AAVHTP+PL SAMGLIAAIL+GDIAVK+G FVNEVVLYMAVASIGM+ATPSYE Sbjct: 391 GVDLLRLAAVHTPTPLASAMGLIAAILIGDIAVKTGLFVNEVVLYMAVASIGMYATPSYE 450 Query: 481 LGXXXXXXXXXXXXXXXXFQVQGFMIGXXXXXXXXXXNRSYNSSYMWPFIPFNAKAMGEI 540 LG F V+GF+IG +RSYNSSY+WPFIPF+AKAMGEI Sbjct: 451 LGLANRMVRLILLLATALFGVKGFVIGTTLLILLLTTHRSYNSSYLWPFIPFSAKAMGEI 510 Query: 541 XXXXPSLCLAPKLGLPSTKQETIHG 565 P L + L T+ T G Sbjct: 511 LFRKPLLLSRRRPALNKTRDNTKMG 535 >B1DJB3.1 B1DJB3_9BACL GerA spore germination protein Length = 547 Score = 566 bits (1459), Expect = e-159, Method: Compositional matrix adjust. Identities = 278/485 (57%), Positives = 360/485 (74%), Gaps = 1/485 (0%) Query: 64 KRDAERSDSLEQSIIYWQGNDN-IPAKLEDTKNTLVEVMGLNSSFDVVFREMTFGGQKAA 122 +R A+ SL + G D+ IP L++ L +G SFD+V REMTFG ++ A Sbjct: 42 ERTAQHDQSLARHEKADGGEDDVIPDSLDEVFERLENEVGYKVSFDIVVREMTFGERRVA 101 Query: 123 LLCISGFAKDIIIDEILKRLTYLTPENISADVLASFMGEYIPHIQVEKGDVLSESITKVL 182 ++G AKD ++ EILKRLTYL P++I+ D F+ Y+P QVEK + +E +T VL Sbjct: 102 FFYMNGLAKDTVLTEILKRLTYLQPDDITGDAFHDFLHLYVPAAQVEKKEKWNEMLTFVL 161 Query: 183 SGMSVFFLEGETEVIIMDTRSYPSRNPDEPSIERVVRGARDGFTETLLSNIALVRRRVRD 242 SG + F++ E + II+D +++P+RNP+EPS+ERVVRG+RDGF ETL++NI+LVRRR+RD Sbjct: 162 SGSTAMFIDKEPQAIIIDAKAFPARNPEEPSLERVVRGSRDGFVETLMTNISLVRRRLRD 221 Query: 243 PGLKNELHQVGRRTRTDVCVAYIDDIVDKTQVKAVTDKIKNVNIDGIPLADKQLEEAIVG 302 P L+ EL +VG RT++D+C+AYI+D+ D VK++ DKIK V+IDG+PLADKQLEEA V Sbjct: 222 PQLRYELLRVGERTQSDICIAYINDLADPELVKSIKDKIKLVHIDGLPLADKQLEEATVQ 281 Query: 303 GGWNPYPMVRYSERPDAVASHLLEGRVVVFVDTSPSVMILPTTFFDLCQHAEENRQTPFM 362 GWNP+P+VRYSERPD VA+HLLEG VVVFVDTSPS M+LPTTFFDL QHAEENRQTPF+ Sbjct: 282 RGWNPFPLVRYSERPDVVAAHLLEGSVVVFVDTSPSAMLLPTTFFDLIQHAEENRQTPFI 341 Query: 363 GTYLRWVRFIGIFASMFLLPLWMLLVIHPELKPPMLDFIGPQKMAKIPLLAQFLIVELGV 422 GTYLRWVRF+GIF+S+FLLPLW+L V+HPELKP L F+GP+K+ K+PL+ QFLIVE+GV Sbjct: 342 GTYLRWVRFLGIFSSLFLLPLWLLFVVHPELKPQALAFLGPEKIGKLPLIVQFLIVEIGV 401 Query: 423 DLLRMAAVHTPSPLGSAMGLIAAILVGDIAVKSGFFVNEVVLYMAVASIGMFATPSYELG 482 DL+R+A+VHTPS L +A+ L+AAI+VGDIAVK+G F+NEV+LYMA+A+IGMFATPSYELG Sbjct: 402 DLMRLASVHTPSTLATAVSLVAAIMVGDIAVKTGLFINEVILYMAIAAIGMFATPSYELG 461 Query: 483 XXXXXXXXXXXXXXXXFQVQGFMIGXXXXXXXXXXNRSYNSSYMWPFIPFNAKAMGEIXX 542 F GF+I RS+N YMWPFIPF+ +AM I Sbjct: 462 LANRIVRLVLIVAVSLFNAPGFVIATTAILMMLCMQRSFNRPYMWPFIPFDYRAMMNIIL 521 Query: 543 XXPSL 547 P L Sbjct: 522 RKPVL 526 >C0GDZ6.1 C0GDZ6_9FIRM GerA spore germination protein Length = 510 Score = 461 bits (1187), Expect = e-128, Method: Compositional matrix adjust. Identities = 218/462 (47%), Positives = 304/462 (65%) Query: 84 DNIPAKLEDTKNTLVEVMGLNSSFDVVFREMTFGGQKAALLCISGFAKDIIIDEILKRLT 143 + + LE+ + E +G+ S DV+ + +T GG+K AL+ I GF ++ IL+ LT Sbjct: 10 ETVSPHLEENLDFFTEKLGIGISIDVLVKGLTIGGKKGALVFIDGFTNGDVVTLILQNLT 69 Query: 144 YLTPENISADVLASFMGEYIPHIQVEKGDVLSESITKVLSGMSVFFLEGETEVIIMDTRS 203 L E+I D IP++++E D LSE + ++L G + FL+GE E IIMD R Sbjct: 70 QLNREDIFPDPFTKLTRVKIPYLEIETSDQLSELMDQMLVGQMIIFLDGEKEAIIMDVRE 129 Query: 204 YPSRNPDEPSIERVVRGARDGFTETLLSNIALVRRRVRDPGLKNELHQVGRRTRTDVCVA 263 +P R P+EP ER+ RG+RDGF ETL+ NIAL RRRVRDPGL+ E + GRR++TDV + Sbjct: 130 FPVRGPEEPDTERLTRGSRDGFVETLMFNIALTRRRVRDPGLRVEAIKAGRRSKTDVAIL 189 Query: 264 YIDDIVDKTQVKAVTDKIKNVNIDGIPLADKQLEEAIVGGGWNPYPMVRYSERPDAVASH 323 YI+DI D V+ V ++ N++IDG+P+A+K +EE IV G W+ +P+VRY+ERPD A H Sbjct: 190 YINDIADPELVEEVKTRLDNIDIDGLPMAEKSIEEFIVEGRWSIFPLVRYTERPDVAAMH 249 Query: 324 LLEGRVVVFVDTSPSVMILPTTFFDLCQHAEENRQTPFMGTYLRWVRFIGIFASMFLLPL 383 L EG V++ VDTSP+ +I P TFF QHAEE RQ+ G Y+RW+RF+GIF SMFL PL Sbjct: 250 LYEGHVIMMVDTSPAAIIAPATFFHHVQHAEEYRQSILSGVYVRWIRFLGIFISMFLAPL 309 Query: 384 WMLLVIHPELKPPMLDFIGPQKMAKIPLLAQFLIVELGVDLLRMAAVHTPSPLGSAMGLI 443 ++L+ +HPEL P MLDFIGP+++ +PL QF+ V LG+DL+RMA VHTP PL +AMGLI Sbjct: 310 YLLVSMHPELLPEMLDFIGPEEVGNVPLFIQFIFVHLGIDLIRMAGVHTPDPLATAMGLI 369 Query: 444 AAILVGDIAVKSGFFVNEVVLYMAVASIGMFATPSYELGXXXXXXXXXXXXXXXXFQVQG 503 AA+L+G+IA++ G F EV+LY + ++GMF+TPSYEL F++ G Sbjct: 370 AALLIGEIAIEVGIFTPEVLLYGGLTAVGMFSTPSYELSLSNRLVHLFMLVVTGLFRLPG 429 Query: 504 FMIGXXXXXXXXXXNRSYNSSYMWPFIPFNAKAMGEIXXXXP 545 F+ G N+S+ Y+WP +PFN KA+ I P Sbjct: 430 FIGGLVIIFIRLATNKSFGVPYLWPLLPFNMKALWSIIMRRP 471 >A6TT32.1 A6TT32_ALKMQ GerA spore germination protein Length = 488 Score = 449 bits (1156), Expect = e-124, Method: Compositional matrix adjust. Identities = 212/467 (45%), Positives = 305/467 (65%), Gaps = 2/467 (0%) Query: 81 QGNDNIP--AKLEDTKNTLVEVMGLNSSFDVVFREMTFGGQKAALLCISGFAKDIIIDEI 138 +G++ +P N L++ + ++ +FD++ R++ + +K L + GFA + ++ I Sbjct: 3 KGDNKVPLSKNFSHNINYLLKELRVDENFDIIHRQLEYANRKFGLFFVDGFANENVMVMI 62 Query: 139 LKRLTYLTPENISADVLASFMGEYIPHIQVEKGDVLSESITKVLSGMSVFFLEGETEVII 198 ++ L +L P +I D + + +IPHI+VEK D L ESIT++LSG +V +EG E I+ Sbjct: 63 MRELEHLAPNDIYKDAVDKLVKRFIPHIEVEKIDDLEESITQILSGQAVLIIEGCNEGIL 122 Query: 199 MDTRSYPSRNPDEPSIERVVRGARDGFTETLLSNIALVRRRVRDPGLKNELHQVGRRTRT 258 +D R YPSR+P+EP IERVVRG RDGF ETL+ N+AL+RRRVRD L E QVG R++T Sbjct: 123 IDIREYPSRSPEEPDIERVVRGPRDGFVETLVFNVALIRRRVRDRSLIMEPLQVGTRSKT 182 Query: 259 DVCVAYIDDIVDKTQVKAVTDKIKNVNIDGIPLADKQLEEAIVGGGWNPYPMVRYSERPD 318 D+ +AY+D +VD V + KI++++IDG+P+ +K LEE I G +NPYPMVRY+ER D Sbjct: 183 DIVIAYLDSVVDLDLVAQLKQKIEDISIDGLPMEEKSLEEFIFGRHYNPYPMVRYTERAD 242 Query: 319 AVASHLLEGRVVVFVDTSPSVMILPTTFFDLCQHAEENRQTPFMGTYLRWVRFIGIFASM 378 HL EG +++ VD SPSVMI P TF+ QHAEE RQ P +G +LRWVRF +FAS+ Sbjct: 243 TCGVHLREGHILMLVDGSPSVMICPATFWHHLQHAEEYRQKPIVGMFLRWVRFTAVFASL 302 Query: 379 FLLPLWMLLVIHPELKPPMLDFIGPQKMAKIPLLAQFLIVELGVDLLRMAAVHTPSPLGS 438 FLLPLW +L +P L P L FIGP+ ++PL QF + E+G+++LRMAA+HTPS L + Sbjct: 303 FLLPLWYVLAENPHLLPESLKFIGPETTGQVPLFWQFFLAEIGIEILRMAAIHTPSALAT 362 Query: 439 AMGLIAAILVGDIAVKSGFFVNEVVLYMAVASIGMFATPSYELGXXXXXXXXXXXXXXXX 498 A+GL+AAIL+G++A+ G F EVVLY+A+A++ FATPSYEL Sbjct: 363 ALGLVAAILIGEVAINVGLFAEEVVLYLAIAAMSTFATPSYELSLANRMFRLVFLIGGAL 422 Query: 499 FQVQGFMIGXXXXXXXXXXNRSYNSSYMWPFIPFNAKAMGEIXXXXP 545 F + GF+ ++S Y+WP IPFN KA+ ++ P Sbjct: 423 FGIYGFLGSILLWIILLATSKSLGVPYLWPLIPFNGKALLDLFIRIP 469 >Q0AZ96.1 Q0AZ96_SYNWW GerA spore germination protein Length = 500 Score = 449 bits (1154), Expect = e-124, Method: Compositional matrix adjust. Identities = 205/441 (46%), Positives = 296/441 (67%), Gaps = 1/441 (0%) Query: 106 SFDVVFREMTFGGQKAALLCISGFAKDIIIDEILKRLTYLTPENISADVLASFMGEYIPH 165 +FD++ RE+ GG+KAA +C+ GF KD I+ E +K L L E + + S + + +P+ Sbjct: 30 NFDLIVREVRVGGRKAAFICLDGFVKDDIMTECMKSLLALKKEVFLSSTVKSILEKGVPY 89 Query: 166 IQVEKGDVLSESITKVLSGMSVFFLEGETEVIIMDTRSYPSRNPDEPSIERVVRGARDGF 225 +++ + + E + VLSG +F L+G E I+D R+YP+RNP+EP IERVVRG+RDGF Sbjct: 90 VELSTENKIDELLYSVLSGSVLFILDGSEEAFIIDPRTYPARNPEEPDIERVVRGSRDGF 149 Query: 226 TETLLSNIALVRRRVRDPGLKNELHQVGRRTRTDVCVAYIDDIVDKTQVKAVTDKIKNVN 285 TET++ N AL+RRRVRDP L+ EL Q G+R++TD+CV Y+ D+ D V+ + KI + Sbjct: 150 TETIVFNTALLRRRVRDPKLRIELLQAGKRSKTDICVCYLKDVADPALVEDIKSKISGIA 209 Query: 286 IDGIPLADKQLEEAIV-GGGWNPYPMVRYSERPDAVASHLLEGRVVVFVDTSPSVMILPT 344 ID +P+A+K +EE I+ G WNP+P VRY+ERPD A HL EG V++ VDTSPSV+ILP Sbjct: 210 IDALPMAEKSVEELIMPGNWWNPFPKVRYTERPDVAAVHLFEGHVLIMVDTSPSVIILPV 269 Query: 345 TFFDLCQHAEENRQTPFMGTYLRWVRFIGIFASMFLLPLWMLLVIHPELKPPMLDFIGPQ 404 TFF QHAEE RQ+P +G Y+RWVRFI + AS+ + PLW+LL + PE+ P L FIGP+ Sbjct: 270 TFFHHLQHAEEYRQSPSVGVYIRWVRFIAVLASIIITPLWLLLALSPEILPASLKFIGPK 329 Query: 405 KMAKIPLLAQFLIVELGVDLLRMAAVHTPSPLGSAMGLIAAILVGDIAVKSGFFVNEVVL 464 ++ +P+ QF+I E+ +D++RMAA+HTPSPL +A+G+IAA+L+GD+AVK G V EV+L Sbjct: 330 EVGNVPIFLQFVIAEISLDMMRMAAIHTPSPLATALGVIAALLIGDVAVKIGLLVPEVIL 389 Query: 465 YMAVASIGMFATPSYELGXXXXXXXXXXXXXXXXFQVQGFMIGXXXXXXXXXXNRSYNSS 524 Y A+ ++G + TPSYE+ F++ GF++ +S Sbjct: 390 YTAMVAVGSYCTPSYEMAMANRLMRFFILLGTGFFRLPGFIVTSILVILLAAFTKSLKVP 449 Query: 525 YMWPFIPFNAKAMGEIXXXXP 545 Y+WP IPFN +A+ I P Sbjct: 450 YLWPLIPFNYRALKGILVRSP 470 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,890,806,408 Number of extensions: 114743334 Number of successful extensions: 269527 Number of sequences better than 1.0e-05: 1082 Number of HSP's gapped: 267294 Number of HSP's successfully gapped: 1083 Length of query: 602 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 460 Effective length of database: 1,722,912,073 Effective search space: 792539553580 Effective search space used: 792539553580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)