BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0212.0007.p (247 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DE04.1 B1DE04_9BACL Conserved hypothetical integral membrane p... 205 3e-51 B3KGQ2.1 B3KGQ2_9BACI Conserved hypothetical integral membrane p... 191 3e-47 C0ZFW2.1 C0ZFW2_BREBN Conserved hypothetical membrane protein 189 2e-46 C2W4E3.1 C2W4E3_BACCE ABC transporter, permease protein 169 2e-40 Q4MWQ7.1 Q4MWQ7_BACCE Putative uncharacterized protein 166 1e-39 >B1DE04.1 B1DE04_9BACL Conserved hypothetical integral membrane protein Length = 239 Score = 205 bits (522), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 107/217 (49%), Positives = 143/217 (65%) Query: 4 NAFFDRQHDTDFIMFSVSHWIAVSLVACFCVILFGLRRTIRSNPGLKQSVRLLLVAVLLF 63 +FF R + +FI +S SH + ++ + ++L+G R IRSN +K +R ++ LL Sbjct: 2 TSFFARYVEEEFIPYSSSHTVMLAALVVLALLLYGFRARIRSNTPVKTIIRYSILLGLLA 61 Query: 64 SEGGLQLWYITQEVWKKGNSLPLELCGITLLLSIVMLLTRSRLLYSFLYFAGIGGAFIAL 123 + L WY +W +LPLELC I+ +L+I+ML TRS+LLY ++FAGIGGA A+ Sbjct: 62 PQILLYYWYAIVGLWSVKYTLPLELCSISQMLAIIMLFTRSKLLYQIVFFAGIGGAMQAM 121 Query: 124 LTPNLVYPFPHFRFLLFFAAHGGIILASLYMTWIEGYKPTWKSLFFTMLCLNIVAAAVYA 183 LTP+L YP+PHFRF FF H IILA LYMTWIE ++PTWKS++ TM+ LNI+ V Sbjct: 122 LTPDLDYPYPHFRFFHFFIVHIAIILAPLYMTWIESFRPTWKSVWITMVFLNILLVVVGG 181 Query: 184 ADRILGANYMFLAHKPGTLSVLDYFGPYPYYLLVEEA 220 D L ANYMFL KP S+LD GPYPYYL+VEE Sbjct: 182 TDYWLDANYMFLREKPEGASLLDLLGPYPYYLVVEEG 218 >B3KGQ2.1 B3KGQ2_9BACI Conserved hypothetical integral membrane protein Length = 273 Score = 191 bits (486), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 109/216 (50%), Positives = 144/216 (66%) Query: 4 NAFFDRQHDTDFIMFSVSHWIAVSLVACFCVILFGLRRTIRSNPGLKQSVRLLLVAVLLF 63 ++FD Q+ F FSV HWIA+ + ++L+ R I S P L+++VR L+A L Sbjct: 19 ESYFDPQYPEAFAAFSVEHWIAIGICIILGLLLYLFRTAIASKPSLQKTVRWGLLAALAL 78 Query: 64 SEGGLQLWYITQEVWKKGNSLPLELCGITLLLSIVMLLTRSRLLYSFLYFAGIGGAFIAL 123 E L +WY++ +W SLPLELC +TL LSI +LLT R LY +YFAGIGGA A+ Sbjct: 79 PEASLHVWYVSTGIWDPATSLPLELCSLTLFLSIAILLTDKRRLYPLVYFAGIGGALQAV 138 Query: 124 LTPNLVYPFPHFRFLLFFAAHGGIILASLYMTWIEGYKPTWKSLFFTMLCLNIVAAAVYA 183 LTPNL YPFPHFRF FF H II+ +LYMTW+ GY+PTWKS+ +TM+ LN+ A V Sbjct: 139 LTPNLGYPFPHFRFFHFFVVHIAIIMTALYMTWVRGYRPTWKSIAWTMVFLNVSALLVGL 198 Query: 184 ADRILGANYMFLAHKPGTLSVLDYFGPYPYYLLVEE 219 + +G+NYMFL KP T S+LD GP+P Y++VEE Sbjct: 199 VNLAVGSNYMFLMRKPDTASLLDLLGPHPVYIVVEE 234 >C0ZFW2.1 C0ZFW2_BREBN Conserved hypothetical membrane protein Length = 248 Score = 189 bits (480), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 98/206 (47%), Positives = 137/206 (66%), Gaps = 1/206 (0%) Query: 15 FIMFSVSHWIAVSLVACFCVILFGLRRTIRSNPGLKQSVRLLLVAVLLFSEGGLQLWYIT 74 F +FS SH I + LV C ++ + R+ +++ P + + R +L VLL SE QLW++ Sbjct: 15 FRLFSSSHVITLILVLCLVMLTYLFRQKLKA-PTHRLTTRYVLAGVLLLSEVCYQLWHVY 73 Query: 75 QEVWKKGNSLPLELCGITLLLSIVMLLTRSRLLYSFLYFAGIGGAFIALLTPNLVYPFPH 134 E W +LPL+LC +TLLLS VML+ RS LY +FAGIGGA ALLTP L YPFPH Sbjct: 74 TESWTAAYTLPLQLCSVTLLLSAVMLMMRSYRLYEITFFAGIGGAMQALLTPELFYPFPH 133 Query: 135 FRFLLFFAAHGGIILASLYMTWIEGYKPTWKSLFFTMLCLNIVAAAVYAADRILGANYMF 194 FRF+ FF AH GI+LA LYMTW+EGY+PT +S++ TM LN++ ++ G NY+F Sbjct: 134 FRFVHFFVAHAGIVLACLYMTWVEGYRPTVRSIWKTMGFLNLLLLVALFVNQWTGGNYLF 193 Query: 195 LAHKPGTLSVLDYFGPYPYYLLVEEA 220 ++ KP S++D+ GPYP+Y++ E Sbjct: 194 VSRKPDNPSLIDFLGPYPWYIVSLEG 219 >C2W4E3.1 C2W4E3_BACCE ABC transporter, permease protein Length = 244 Score = 169 bits (428), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 91/219 (41%), Positives = 135/219 (61%), Gaps = 7/219 (3%) Query: 4 NAFFDRQHDTDFIMFSVSHWIAVSLVACFCVILFGLRRTIRS---NPGLKQSVRLLLVAV 60 A+F F+ +S H I + L+ L+ + +R N G++ ++ L + Sbjct: 2 EAYFGAFPSEAFVPYSKQHAIILFLIGVGVYFLYHYQDVLREQQWNVGVRYTIAFLFIG- 60 Query: 61 LLFSEGGLQLWYITQEVWKKGNSLPLELCGITLLLSIVMLLTRSRLLYSFLYFAGIGGAF 120 SE GL +W + +++ SLPLELC I+LLL+ VM++T+S +Y ++F GI GA Sbjct: 61 ---SEIGLDVWQVKAGIFQLSTSLPLELCTISLLLATVMVITKSYKIYEIVFFTGIIGAS 117 Query: 121 IALLTPNLVYPFPHFRFLLFFAAHGGIILASLYMTWIEGYKPTWKSLFFTMLCLNIVAAA 180 A+LTPNL Y FPHFRF+ +F AH +I A L+MTW+EGY+PT++S+ TM+ LN + Sbjct: 118 QAILTPNLQYAFPHFRFIEYFIAHVLLIWAPLFMTWVEGYRPTFQSIKRTMIFLNALIPI 177 Query: 181 VYAADRILGANYMFLAHKPGTLSVLDYFGPYPYYLLVEE 219 V + G NYMFLAHKP T+S+LD GP+PYY++ E Sbjct: 178 VSFVNYKTGGNYMFLAHKPETVSLLDMLGPHPYYIISLE 216 >Q4MWQ7.1 Q4MWQ7_BACCE Putative uncharacterized protein Length = 244 Score = 166 bits (421), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 91/216 (42%), Positives = 132/216 (61%), Gaps = 1/216 (0%) Query: 4 NAFFDRQHDTDFIMFSVSHWIAVSLVACFCVILFGLRRTIRSNPGLKQSVRLLLVAVLLF 63 A+F FI +S H I + ++ V L+ + +R N +VR + + + Sbjct: 2 EAYFSAHPLKPFIPYSRQHAIILFIMLVGIVFLYQYQDVLRQNEW-NITVRYAIAFLFIG 60 Query: 64 SEGGLQLWYITQEVWKKGNSLPLELCGITLLLSIVMLLTRSRLLYSFLYFAGIGGAFIAL 123 SE GL +W +++ SLP ELC I+LLL+ +M++T+S +Y ++F GI GA A+ Sbjct: 61 SEIGLHMWEWKARIFELATSLPFELCTISLLLASIMIVTKSYRIYEIVFFTGIIGASQAI 120 Query: 124 LTPNLVYPFPHFRFLLFFAAHGGIILASLYMTWIEGYKPTWKSLFFTMLCLNIVAAAVYA 183 LTPNL Y FPHFRF+ +F AH +I A L+MTW+EGY+PT +S+ TM+ LNI+ V Sbjct: 121 LTPNLQYAFPHFRFIEYFIAHILLIWAPLFMTWVEGYRPTVQSIKRTMIFLNILIPLVSF 180 Query: 184 ADRILGANYMFLAHKPGTLSVLDYFGPYPYYLLVEE 219 + G NYMFLAHKP T S+LD GP+PYY++ E Sbjct: 181 VNYKTGGNYMFLAHKPETASLLDMLGPHPYYIISLE 216 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.145 0.463 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,417,344,755 Number of extensions: 54731706 Number of successful extensions: 156727 Number of sequences better than 1.0e-05: 152 Number of HSP's gapped: 156648 Number of HSP's successfully gapped: 152 Length of query: 247 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 115 Effective length of database: 1,817,122,223 Effective search space: 208969055645 Effective search space used: 208969055645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)