BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0016.p (488 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIT6.1 B3KIT6_9BACI 2-oxoglutarate dehydrogenase, E1 subunit E... 585 e-165 B1DDC1.1 B1DDC1_9BACL 2-oxoglutarate dehydrogenase, E1 subunit E... 580 e-164 C2QQ07.1 C2QQ07_BACCE 2-oxoglutarate dehydrogenase E1 component 555 e-156 C3A2Z5.1 C3A2Z5_BACMY 2-oxoglutarate dehydrogenase E1 component 555 e-156 C3BHI2.1 C3BHI2_9BACI 2-oxoglutarate dehydrogenase E1 component 554 e-156 >B3KIT6.1 B3KIT6_9BACI 2-oxoglutarate dehydrogenase, E1 subunit EC=1.2.4.2 Length = 958 Score = 585 bits (1509), Expect = e-165, Method: Compositional matrix adjust. Identities = 274/473 (57%), Positives = 354/473 (74%) Query: 2 DDPETTQPIVYSKVRNHPTVYRVYAERLQRDNIIKADDVKQMNLEAEKVLQQAYDLMKDG 61 DDPETTQP++Y KV+NHPTV +Y ++L NI+ + V + +VL+ +Y+ +K Sbjct: 473 DDPETTQPLIYQKVKNHPTVSAIYMKKLVDGNILTQEQVDGLKNGVVEVLKNSYENVKTK 532 Query: 62 KHKNGETKTSVPVQSEASQPQSTAVQLEQLQRINRELLTVPDGFKVYPKLERILQRRKDA 121 + P ++ +T V E L+ INRELL P+GF VYPKL+RIL+RR ++ Sbjct: 533 DPVDQILLGLNPTDGKSKTEAATNVSAEVLRGINRELLQWPEGFTVYPKLQRILERRSNS 592 Query: 122 LNDGERIDWALAETLAFATILKDGTPIRLSGQDSQRGTFAHRHLVLHDSETGGLFSPLHQ 181 L++GE++DW AETLAFATIL DG PIR++GQD++R TFAHR+LVLHD+ G F PLH+ Sbjct: 593 LDEGEKVDWGHAETLAFATILADGKPIRITGQDAERATFAHRNLVLHDAVNGETFCPLHR 652 Query: 182 LSDAKASFGVYNSPLSEASVLGYEYGYNVFAPETLVIWEAQYGDFANAAQVIFDQFIAAG 241 L AKASF ++NSPL+EA+V+G+EYGYNV++PETLVIWEAQYGDF N AQVI DQFI AG Sbjct: 653 LPQAKASFAIHNSPLTEAAVVGFEYGYNVYSPETLVIWEAQYGDFVNCAQVIIDQFITAG 712 Query: 242 RAKWTQRSNLTILLPHGYEGQGPEHSSARLERYLQLSAEENWTVANLSSAAQYFHLLRRQ 301 R+KW Q+S+L +LLPHGYEG GPEHSSARLER+LQLS E+NWTVANL+SAAQYFHLLR+Q Sbjct: 713 RSKWRQKSSLVMLLPHGYEGNGPEHSSARLERFLQLSGEDNWTVANLTSAAQYFHLLRKQ 772 Query: 302 AALCGKSDARPLVIMAPKSLIRNPRSTSSGAELASGEFQXXXXXXXXXXXXXXVKRLVVC 361 A+L DA+PLV+MAPKSLIRNPR S + G FQ V+RLV+C Sbjct: 773 ASLTNTDDAKPLVVMAPKSLIRNPRVASPVNAFSEGGFQKVMEQPGLGQTPKKVERLVLC 832 Query: 362 SGKVTLDLQTELEAGKDQDWSWLHILRLEQLYPFPERELAAHLSALGSLTEIVWVQEEPK 421 SGK+ +DL+ L+ K +D SW+HILR+EQLYPFP+ E++ + +L EI+WVQEEP+ Sbjct: 833 SGKIAIDLEDALDKQKGEDQSWIHILRVEQLYPFPKEEISRVMQKYPNLKEILWVQEEPQ 892 Query: 422 NMGGWSYAEPRLRAIAPQNVKVQYIGRPERSSPASGYADVHTFEQRRIVTEAL 474 NMGGWS+ EPR+R IAP+ +V+Y GRPERSSPASGY DVH +EQ+ I++ AL Sbjct: 893 NMGGWSFMEPRIREIAPKGTEVRYSGRPERSSPASGYQDVHAYEQQTIISFAL 945 >B1DDC1.1 B1DDC1_9BACL 2-oxoglutarate dehydrogenase, E1 subunit EC=1.2.4.2 Length = 960 Score = 580 bits (1496), Expect = e-164, Method: Compositional matrix adjust. Identities = 278/474 (58%), Positives = 348/474 (73%) Query: 2 DDPETTQPIVYSKVRNHPTVYRVYAERLQRDNIIKADDVKQMNLEAEKVLQQAYDLMKDG 61 DDPETTQP +Y KV+ HPTV RV+ ++L + I + + + E L+ AY+ MK Sbjct: 476 DDPETTQPTIYKKVKAHPTVSRVFTDKLVAEGAITEQEAEGLKQEVIGRLKAAYEEMKSR 535 Query: 62 KHKNGETKTSVPVQSEASQPQSTAVQLEQLQRINRELLTVPDGFKVYPKLERILQRRKDA 121 + + +T A+ + +E L+ IN LL P+ F VYPKL+RIL+RR A Sbjct: 536 EGQEPVHQTQGLPNDLATGDAVAPISVETLREINANLLKWPETFTVYPKLQRILERRATA 595 Query: 122 LNDGERIDWALAETLAFATILKDGTPIRLSGQDSQRGTFAHRHLVLHDSETGGLFSPLHQ 181 +NDGE++DW AETLAFATIL +G PIRLSGQD +R TFAHR+LVLHD+ETG F PLH Sbjct: 596 VNDGEKMDWGHAETLAFATILAEGKPIRLSGQDVERATFAHRNLVLHDAETGDTFCPLHH 655 Query: 182 LSDAKASFGVYNSPLSEASVLGYEYGYNVFAPETLVIWEAQYGDFANAAQVIFDQFIAAG 241 L +AKASF ++NSPLSEA+VLG+EYGYNV APET+VIWEAQYGDF N AQV+ DQFI+AG Sbjct: 656 LPEAKASFAIHNSPLSEAAVLGFEYGYNVHAPETMVIWEAQYGDFTNVAQVLIDQFISAG 715 Query: 242 RAKWTQRSNLTILLPHGYEGQGPEHSSARLERYLQLSAEENWTVANLSSAAQYFHLLRRQ 301 RAKW Q+S+L ++LPHGYEGQGPEHSSARLER+LQLSAEENWTV NLSSAA YFHLLRRQ Sbjct: 716 RAKWQQKSSLIMMLPHGYEGQGPEHSSARLERFLQLSAEENWTVVNLSSAANYFHLLRRQ 775 Query: 302 AALCGKSDARPLVIMAPKSLIRNPRSTSSGAELASGEFQXXXXXXXXXXXXXXVKRLVVC 361 A++ ARPL+IMAPKSLIRNPR + +EL+SG F+ V+RLV+ Sbjct: 776 ASITESDAARPLIIMAPKSLIRNPRVAAEISELSSGSFKAVIEQKGLGAKPDRVERLVLA 835 Query: 362 SGKVTLDLQTELEAGKDQDWSWLHILRLEQLYPFPERELAAHLSALGSLTEIVWVQEEPK 421 +GK+ +D++ +E DQ+ WLH++R+EQLYPFPE+E+AA + L +L EIVWVQEEP+ Sbjct: 836 TGKMAIDIEEAIEKNGDQELDWLHVVRVEQLYPFPEKEIAAIIERLPNLKEIVWVQEEPQ 895 Query: 422 NMGGWSYAEPRLRAIAPQNVKVQYIGRPERSSPASGYADVHTFEQRRIVTEALK 475 NMG W Y EPRLR IAP V YIGRPERSS ASG+ VH+FEQ+ I+ + LK Sbjct: 896 NMGSWFYMEPRLRKIAPAAASVSYIGRPERSSTASGFQQVHSFEQQHILLQTLK 949 >C2QQ07.1 C2QQ07_BACCE 2-oxoglutarate dehydrogenase E1 component Length = 955 Score = 555 bits (1431), Expect = e-156, Method: Compositional matrix adjust. Identities = 262/478 (54%), Positives = 350/478 (73%), Gaps = 2/478 (0%) Query: 1 MDDPETTQPIVYSKVRNHPTVYRVYAERLQRDNIIKADDVKQMNLEAEKVLQQAYDLMKD 60 MDDP TQP VY K++NHPTV +YA++LQ ++ AD+++ + ++ L+ Y + Sbjct: 462 MDDPAVTQPQVYKKIKNHPTVRAIYADQLQAAGVLNADEIETITQFTQEQLKSDYAQVPP 521 Query: 61 GKHKNGETKTSVP-VQSEASQPQSTAVQLEQLQRINRELLTVPDGFKVYPKLERILQRRK 119 + VP V ++ QP T V+L+ L+ IN LL+ P+GF VYPK+++IL+RRK Sbjct: 522 ADTSDATIHVKVPDVVAKGIQPIDTGVELDSLRAINEGLLSWPEGFNVYPKVKKILERRK 581 Query: 120 DALNDGERIDWALAETLAFATILKDGTPIRLSGQDSQRGTFAHRHLVLHDSETGGLFSPL 179 DAL + +I+WALAE+LAFA+IL++GTPIRL+GQDSQRGTFAHRH+VLHD++T +SPL Sbjct: 582 DALEENGKIEWALAESLAFASILQEGTPIRLTGQDSQRGTFAHRHIVLHDTDTNETYSPL 641 Query: 180 HQLSDAKASFGVYNSPLSEASVLGYEYGYNVFAPETLVIWEAQYGDFANAAQVIFDQFIA 239 H+L + ASF V+NSPLSEA+V+GYEYGYNVFAPETLV+WEAQYGDF+N AQ +FDQ+++ Sbjct: 642 HRLPNINASFSVHNSPLSEAAVVGYEYGYNVFAPETLVMWEAQYGDFSNTAQALFDQYVS 701 Query: 240 AGRAKWTQRSNLTILLPHGYEGQGPEHSSARLERYLQLSAEENWTVANLSSAAQYFHLLR 299 AGRAKW Q+S L +LLPHGYEGQGPEHSSAR ER+LQL+AE NWTVANL+SAAQYFH+LR Sbjct: 702 AGRAKWGQKSGLVLLLPHGYEGQGPEHSSARPERFLQLAAENNWTVANLTSAAQYFHILR 761 Query: 300 RQAALCGKSDARPLVIMAPKSLIRNPRSTSSGAELASGEFQXXXXXXXXXXXXXXVKRLV 359 RQA++ G RPLV+M PKSL+R+P + S+ ++L+ G FQ VKRLV Sbjct: 762 RQASILGTEAVRPLVLMTPKSLLRHPLTLSTASQLSEGRFQPALEQENLGTKPNKVKRLV 821 Query: 360 VCSGKVTLDLQTELEAGKDQ-DWSWLHILRLEQLYPFPERELAAHLSALGSLTEIVWVQE 418 + +GK+ +DL TE+E+GK + + +HI+R+EQLYPFP ++ + + +L EI+WVQE Sbjct: 822 LSTGKMAIDLATEIESGKHEYNLDEVHIVRIEQLYPFPAEKVQSIIKRFKNLEEIIWVQE 881 Query: 419 EPKNMGGWSYAEPRLRAIAPQNVKVQYIGRPERSSPASGYADVHTFEQRRIVTEALKL 476 EP+NMG W Y P L +A VK YIGRP+RSSP+ G H EQ IV AL + Sbjct: 882 EPRNMGAWHYMAPILFELAGDKVKTGYIGRPDRSSPSGGDPFAHKAEQELIVAHALDV 939 >C3A2Z5.1 C3A2Z5_BACMY 2-oxoglutarate dehydrogenase E1 component Length = 955 Score = 555 bits (1430), Expect = e-156, Method: Compositional matrix adjust. Identities = 264/478 (55%), Positives = 347/478 (72%), Gaps = 2/478 (0%) Query: 1 MDDPETTQPIVYSKVRNHPTVYRVYAERLQRDNIIKADDVKQMNLEAEKVLQQAYDLMKD 60 MDDP TQP VY K++NHPTV +YA++LQ ++ AD+V+ + ++ L+ Y + Sbjct: 462 MDDPAVTQPQVYKKIKNHPTVRAIYADQLQSAGVLNADEVETITQFIQEELKAEYAQVPP 521 Query: 61 GKHKNGETKTSVP-VQSEASQPQSTAVQLEQLQRINRELLTVPDGFKVYPKLERILQRRK 119 VP V ++ QP T V +E L+ IN LL+ P+GF VYPK+++IL+RRK Sbjct: 522 ADTSAATIHVKVPEVVAKGIQPIDTGVSIESLRTINEGLLSWPEGFNVYPKVKKILERRK 581 Query: 120 DALNDGERIDWALAETLAFATILKDGTPIRLSGQDSQRGTFAHRHLVLHDSETGGLFSPL 179 DAL + +I+WALAE+LAFA+IL++GTPIRL+GQDSQRGTFAHRH+VLHD++T +SPL Sbjct: 582 DALEENGKIEWALAESLAFASILQEGTPIRLTGQDSQRGTFAHRHIVLHDTDTNETYSPL 641 Query: 180 HQLSDAKASFGVYNSPLSEASVLGYEYGYNVFAPETLVIWEAQYGDFANAAQVIFDQFIA 239 H+L + ASF V+NSPLSEA+V+GYEYGYNVFAPETLV+WEAQYGDF+N AQ +FDQ+++ Sbjct: 642 HRLPNINASFSVHNSPLSEAAVVGYEYGYNVFAPETLVMWEAQYGDFSNTAQALFDQYVS 701 Query: 240 AGRAKWTQRSNLTILLPHGYEGQGPEHSSARLERYLQLSAEENWTVANLSSAAQYFHLLR 299 AGRAKW Q+S L +LLPHGYEGQGPEHSSAR ER+LQL+AE NWTVANL+SAAQYFH+LR Sbjct: 702 AGRAKWGQKSGLVLLLPHGYEGQGPEHSSARPERFLQLAAENNWTVANLTSAAQYFHILR 761 Query: 300 RQAALCGKSDARPLVIMAPKSLIRNPRSTSSGAELASGEFQXXXXXXXXXXXXXXVKRLV 359 RQA++ G RPLVIM PKSL+R+P + S+G+EL+ G FQ VKRLV Sbjct: 762 RQASILGTEAVRPLVIMTPKSLLRHPLTLSTGSELSEGRFQPALEQGNLGAKPNKVKRLV 821 Query: 360 VCSGKVTLDLQTELEAGKDQ-DWSWLHILRLEQLYPFPERELAAHLSALGSLTEIVWVQE 418 + +GK+ +DL E+E+GK + +H++R+EQLYPFP ++ + + +L EI+WVQE Sbjct: 822 LSTGKMAIDLAAEIESGKHEYSLDEVHMVRIEQLYPFPAEKVQSIIKRFKNLEEIIWVQE 881 Query: 419 EPKNMGGWSYAEPRLRAIAPQNVKVQYIGRPERSSPASGYADVHTFEQRRIVTEALKL 476 EP+NMG W Y P L +A VK YIGRP+RSSP+ G H EQ IV AL + Sbjct: 882 EPRNMGAWHYMAPILFELAGDKVKTGYIGRPDRSSPSGGDPFAHKAEQELIVAHALDV 939 >C3BHI2.1 C3BHI2_9BACI 2-oxoglutarate dehydrogenase E1 component Length = 961 Score = 554 bits (1428), Expect = e-156, Method: Compositional matrix adjust. Identities = 264/476 (55%), Positives = 348/476 (73%), Gaps = 2/476 (0%) Query: 1 MDDPETTQPIVYSKVRNHPTVYRVYAERLQRDNIIKADDVKQMNLEAEKVLQQAYDLMKD 60 MDDP TQP VY K++NHPTV +YA +L+ ++ AD+V+ + ++ L+ Y + Sbjct: 468 MDDPAVTQPQVYKKIKNHPTVRALYASQLEAAGVLNADEVETITQFIQEELKAEYAQVPP 527 Query: 61 GKHKNGETKTSVP-VQSEASQPQSTAVQLEQLQRINRELLTVPDGFKVYPKLERILQRRK 119 + VP V ++ QP T + LE L+ IN LLT P+GF VYPK+++IL+RRK Sbjct: 528 ADTSDAAIHVKVPDVVAKGIQPIDTGLPLETLRAINEGLLTWPEGFNVYPKVKKILERRK 587 Query: 120 DALNDGERIDWALAETLAFATILKDGTPIRLSGQDSQRGTFAHRHLVLHDSETGGLFSPL 179 DAL + +I+WALAE+LAFA+IL++GTPIRL+GQDSQRGTFAHRH+VLHD+ET +SPL Sbjct: 588 DALEENGKIEWALAESLAFASILQEGTPIRLTGQDSQRGTFAHRHIVLHDTETNETYSPL 647 Query: 180 HQLSDAKASFGVYNSPLSEASVLGYEYGYNVFAPETLVIWEAQYGDFANAAQVIFDQFIA 239 H+L + +ASF V+NSPLSEA+V+GYEYGYNVFAPETLV+WEAQYGDF+N AQ +FDQ+++ Sbjct: 648 HRLPNIRASFSVHNSPLSEAAVVGYEYGYNVFAPETLVMWEAQYGDFSNTAQALFDQYVS 707 Query: 240 AGRAKWTQRSNLTILLPHGYEGQGPEHSSARLERYLQLSAEENWTVANLSSAAQYFHLLR 299 +GRAKW Q+S L +LLPHGYEGQGPEHSSAR ER+LQL+AE NWTVANL+SAAQYFH+LR Sbjct: 708 SGRAKWGQKSGLVLLLPHGYEGQGPEHSSARPERFLQLAAENNWTVANLTSAAQYFHILR 767 Query: 300 RQAALCGKSDARPLVIMAPKSLIRNPRSTSSGAELASGEFQXXXXXXXXXXXXXXVKRLV 359 RQA++ G RPLVIM PKSL+R+P + SSG++L+ G FQ VKRLV Sbjct: 768 RQASILGTEAVRPLVIMTPKSLLRHPLTASSGSQLSEGRFQPALEQENLGIKPNKVKRLV 827 Query: 360 VCSGKVTLDLQTELEAGKDQ-DWSWLHILRLEQLYPFPERELAAHLSALGSLTEIVWVQE 418 + +GK+ +DL E+E+GK + + +HI+R+EQLYPFP ++ + + +L EI+WVQE Sbjct: 828 LTTGKMAIDLAAEIESGKHEYNLDEVHIVRIEQLYPFPAEKVQSIMKRFKNLEEIIWVQE 887 Query: 419 EPKNMGGWSYAEPRLRAIAPQNVKVQYIGRPERSSPASGYADVHTFEQRRIVTEAL 474 EP+NMG W Y P L +A +K YIGRP+RSSP+ G H EQ IV AL Sbjct: 888 EPRNMGAWHYMAPILFELAGDKLKTGYIGRPDRSSPSGGDPHAHRAEQELIVAHAL 943 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,849,476,546 Number of extensions: 116619564 Number of successful extensions: 274311 Number of sequences better than 1.0e-05: 1240 Number of HSP's gapped: 268353 Number of HSP's successfully gapped: 1257 Length of query: 488 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 348 Effective length of database: 1,741,754,103 Effective search space: 606130427844 Effective search space used: 606130427844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)