BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0015.p (460 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIT6.1 B3KIT6_9BACI 2-oxoglutarate dehydrogenase, E1 subunit E... 593 e-167 B1DDC1.1 B1DDC1_9BACL 2-oxoglutarate dehydrogenase, E1 subunit E... 592 e-167 Q2BAP9.1 Q2BAP9_9BACI OdhA 532 e-149 Q9KAT1.1 ODO1_BACHD 2-oxoglutarate dehydrogenase E1 component EC... 530 e-148 A8FE66.1 ODO1_BACP2 2-oxoglutarate dehydrogenase E1 component EC... 520 e-146 >B3KIT6.1 B3KIT6_9BACI 2-oxoglutarate dehydrogenase, E1 subunit EC=1.2.4.2 Length = 958 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 283/463 (61%), Positives = 346/463 (74%), Gaps = 3/463 (0%) Query: 1 MAVKEPYNESIWSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXX 60 M + E W KYHGPNLGYIQ++YE+F++DP SVE YR+LF G Sbjct: 1 MKTLDRMTEGPWQKYHGPNLGYIQDQYEKFLQDPQSVEPGYRELFASWGPPPSLENNEKK 60 Query: 61 XXXXLSGGT---EWLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLT 117 G + + ++K V A KL+ NIR YGH+AA+IDP+ GN + L E+Y L+ Sbjct: 61 ADSQGQGVSYDPQLMKKGVAAGKLVWNIRTYGHLAADIDPIGIGNEADTRLLQPESYQLS 120 Query: 118 REDLIAMPASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAES 177 + DL A+PA ++WE AP D+ +A+ R+LQ YT +IAYEF HVHDE+E +WLN QAES Sbjct: 121 QADLAALPAEIVWEEAPGDIKNGLEAIQRLLQLYTGSIAYEFSHVHDEEERKWLNQQAES 180 Query: 178 TSSPAPLNNAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAH 237 + ++ ER+ LL +L++ EQFE FLH+TFVG KRFS+EGN+ALVP+LDEIVR A Sbjct: 181 GRYLSDMSVEERRGLLKKLLEAEQFEEFLHRTFVGAKRFSVEGNEALVPLLDEIVRQMAE 240 Query: 238 DGAEHILMGMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVK 297 IL+GMAHRGRLNVLAH+LGK Y IFSEF HSPNK L PSEGSMGINYGWTGDVK Sbjct: 241 ADVNSILVGMAHRGRLNVLAHVLGKDYGKIFSEFMHSPNKHLVPSEGSMGINYGWTGDVK 300 Query: 298 YHLGADRAVREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAM 357 YHLGADR +++ E + RITLANNPSHLE+VNPVVEGFARAAQEDR+ G P+ D NKA+ Sbjct: 301 YHLGADRYLKKDEARQVRITLANNPSHLEYVNPVVEGFARAAQEDRSEAGYPKQDVNKAL 360 Query: 358 AVLMHGDAAFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDL 417 ++ +HGD+AFPGEGIV ETLN KL + GG++HIIVNNRIGFTTE +DSRSTHYASDL Sbjct: 361 SIQIHGDSAFPGEGIVPETLNFNKLPAFRTGGSIHIIVNNRIGFTTEGKDSRSTHYASDL 420 Query: 418 AKGYEIPIVHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 AKGYEIPI+HVNADDPEACIA RLAS YR++FKK +IDL+G Sbjct: 421 AKGYEIPIIHVNADDPEACIAVARLASDYRNKFKKSILIDLVG 463 >B1DDC1.1 B1DDC1_9BACL 2-oxoglutarate dehydrogenase, E1 subunit EC=1.2.4.2 Length = 960 Score = 592 bits (1527), Expect = e-167, Method: Compositional matrix adjust. Identities = 281/455 (61%), Positives = 347/455 (76%), Gaps = 6/455 (1%) Query: 12 WSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGX--XXXXXXXXXXXXXXLSGGT 69 W+ Y+GPNLGY+QE+YE F ++P SV YR+LF G SGG Sbjct: 12 WASYYGPNLGYVQEQYELFKQNPESVVAEYRELFEQYGEPPQYAGQTAAPIVTAQASGGA 71 Query: 70 ----EWLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTREDLIAMP 125 + L+ V A KL+ NIR +GH+AA+IDPL L+ ETY LT DL A+P Sbjct: 72 AIDAKVLQNLVAAGKLVWNIRAFGHLAADIDPLGISPKVSTNMLELETYGLTEADLAALP 131 Query: 126 ASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTSSPAPLN 185 A L+W APADV WDA+ ++ +AYT TIAYEF HVH+E+E WL + E + LN Sbjct: 132 AELVWTKAPADVKNGWDAIEKLRKAYTGTIAYEFSHVHNEEERVWLKQRVEKQNPATKLN 191 Query: 186 NAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDGAEHILM 245 ERK++L +L + EQFE FL +TFVGQKRFS+EGND LV ++DE+V ++DG +HILM Sbjct: 192 TEERKKILTKLFEAEQFEDFLQRTFVGQKRFSVEGNDVLVALVDEMVHELSNDGVKHILM 251 Query: 246 GMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYHLGADRA 305 GMAHRGRLN+LAH+LGKPY IFSEFHH+ NK++ PSEGS+GINYGWTGDVKYHLGA+R+ Sbjct: 252 GMAHRGRLNILAHVLGKPYSKIFSEFHHASNKKMSPSEGSIGINYGWTGDVKYHLGANRS 311 Query: 306 VREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAVLMHGDA 365 + GETV+TRI+LANNPSHLEFVNPVVEGF RAAQ+DR +PG P+ + + A A++MHGDA Sbjct: 312 IGTGETVKTRISLANNPSHLEFVNPVVEGFTRAAQDDRTAPGYPKQNPDLAAAIIMHGDA 371 Query: 366 AFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAKGYEIPI 425 AFPGEGIVAET+N+ +L GYS+GGT+HIIVNN+IGFTTES DSRSTHYASD+AKGYEIPI Sbjct: 372 AFPGEGIVAETMNLSQLNGYSHGGTLHIIVNNKIGFTTESHDSRSTHYASDVAKGYEIPI 431 Query: 426 VHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 VHVNADDP+A IAA R+AS YR++FKKDF+IDLIG Sbjct: 432 VHVNADDPDAVIAAARMASEYRTKFKKDFLIDLIG 466 >Q2BAP9.1 Q2BAP9_9BACI OdhA Length = 946 Score = 532 bits (1371), Expect = e-149, Method: Compositional matrix adjust. Identities = 262/460 (56%), Positives = 333/460 (72%), Gaps = 3/460 (0%) Query: 3 VKEPYN-ESIWSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXXX 61 +K P N E +HGPNLGY+ E YE+F++DP SV+ R+ F G Sbjct: 1 MKTPSNGEGPLQGFHGPNLGYVIELYERFLEDPQSVDAEMREYFEKWGSPEQTQIDTFEN 60 Query: 62 XXXLSGGTE-WLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTRED 120 + L K + A +L NIR YGH++A+I PL + N P L E Y LTR D Sbjct: 61 DKPVQAAAPGQLEKTLAAIRLADNIRAYGHISADIYPL-KNNKPEKDILALEQYGLTRND 119 Query: 121 LIAMPASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTSS 180 L +PAS+I +AP+ V ++A+ + YTQTIAYEF HV+D E WL ++ E+ + Sbjct: 120 LEQVPASIICSDAPSHVRNGFEAIEHLKNVYTQTIAYEFHHVYDVNEKNWLRNKVETGTL 179 Query: 181 PAPLNNAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDGA 240 A +N ++ +L RL++VE+FE FLH+TFVGQKRFS+EG D LVP+LDE+V + DGA Sbjct: 180 LAEPSNEKKVGVLKRLMEVEEFEKFLHRTFVGQKRFSIEGLDTLVPLLDELVAESVVDGA 239 Query: 241 EHILMGMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYHL 300 + I +GMAHRGRLNVLAH+LGKPY++IF+EF H+PNKEL PSEGS+GINYGWTGDVKYHL Sbjct: 240 KTINIGMAHRGRLNVLAHVLGKPYEMIFAEFQHAPNKELVPSEGSIGINYGWTGDVKYHL 299 Query: 301 GADRAVREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAVL 360 G DR +++ T + R+TLANNPSHLEFV VVEGF RAAQ+DR++ G P+ D + AMA+L Sbjct: 300 GLDRQIKKENTTKARLTLANNPSHLEFVGAVVEGFTRAAQDDRSASGYPKEDPSSAMAIL 359 Query: 361 MHGDAAFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAKG 420 +HGDAAFPG+G VAETLN+ +L GY GGT+HII NN IGFTTES+DSRST YASDLAKG Sbjct: 360 IHGDAAFPGQGTVAETLNLSQLTGYRTGGTIHIIANNTIGFTTESQDSRSTRYASDLAKG 419 Query: 421 YEIPIVHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 YEIPI+HVNADDPEA +AA +LA YR++F KDF+IDLIG Sbjct: 420 YEIPILHVNADDPEAVVAAAQLACEYRAKFNKDFLIDLIG 459 >Q9KAT1.1 ODO1_BACHD 2-oxoglutarate dehydrogenase E1 component EC=1.2.4.2 Length = 945 Score = 530 bits (1365), Expect = e-148, Method: Compositional matrix adjust. Identities = 262/464 (56%), Positives = 333/464 (71%), Gaps = 7/464 (1%) Query: 1 MAVKEPYNESIWSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXX 60 M+ KE +E W ++GPNLG E YE++ KDP++V+ R+ F G Sbjct: 1 MSSKEHTSEEPWQGFYGPNLGVAVELYEEYQKDPNAVDDELREAFEKWGPPPATAPERTQ 60 Query: 61 XXXXLSGGTE--WLRKAVRASKLIANIRIYGHMAANIDPL--EQGNNPMAKWLDYETYDL 116 + T+ ++K V A KL +IR +GH+AA+I P+ EQ M D + + L Sbjct: 61 STFTAASITDPDVIKKFVGAVKLADHIRAFGHLAADIQPILKEQRREDM---FDLDRFGL 117 Query: 117 TREDLIAMPASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAE 176 T D+ ++P L+ APA V DA++ + + YT+TIA+EF HV DE+E +WLN E Sbjct: 118 TESDIRSVPVDLLCPYAPAHVKNGLDAINHLKEVYTKTIAFEFVHVTDEEERKWLNRMIE 177 Query: 177 STSSPAPLNNAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAA 236 S L+ +RK LL RL VE FE FLH+TFVGQKRFS+EG D LVPMLDE+VR A Sbjct: 178 SGLYLPNLSTEQRKSLLKRLTDVEGFEKFLHRTFVGQKRFSIEGLDTLVPMLDEVVREAV 237 Query: 237 HDGAEHILMGMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDV 296 H+G ++++GMAHRGRLNVLAH+L KPY++IF+EF HS NK+LFPSEGS+GINYGWTGDV Sbjct: 238 HEGTTNVMIGMAHRGRLNVLAHVLNKPYEMIFAEFLHSLNKDLFPSEGSIGINYGWTGDV 297 Query: 297 KYHLGADRAVREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKA 356 KYHLGADR +R+ T R+TLANNPSHLEFV+P+VEG+ARAAQEDR SPG P+ D KA Sbjct: 298 KYHLGADRQIRDENTAEARLTLANNPSHLEFVSPIVEGYARAAQEDRTSPGAPEQDIMKA 357 Query: 357 MAVLMHGDAAFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASD 416 ++L+HGDAAFPG+G+V ETLN+ +L GY GG++HII NN IGFTTE+ DSRST Y+SD Sbjct: 358 YSILIHGDAAFPGQGVVTETLNLSRLTGYQTGGSLHIIANNNIGFTTETFDSRSTLYSSD 417 Query: 417 LAKGYEIPIVHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 AKG+EIPIVHVNADDPEAC+AAV LA YR +FKKDF+IDLIG Sbjct: 418 PAKGFEIPIVHVNADDPEACLAAVHLAFQYRKRFKKDFLIDLIG 461 >A8FE66.1 ODO1_BACP2 2-oxoglutarate dehydrogenase E1 component EC=1.2.4.2 Length = 944 Score = 520 bits (1340), Expect = e-146, Method: Compositional matrix adjust. Identities = 252/458 (55%), Positives = 324/458 (70%), Gaps = 4/458 (0%) Query: 3 VKEPYNESIWSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXXXX 62 VK+P + W ++HGPNLGY+ E Y+Q+V+DP+SV+ R +F G Sbjct: 6 VKQPLS---WEEFHGPNLGYVLELYDQYVQDPTSVDEDLRGIFDELGAPPSEMKEEIGKK 62 Query: 63 XXLSGGTEWLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTREDLI 122 +E ++K KL +IR YGH+ A+++PL + + + + Y LT ED+ Sbjct: 63 ENSVVTSEQIQKIASVVKLAEDIRTYGHLNASVNPLRK-EKELQELFPLKEYGLTEEDVK 121 Query: 123 AMPASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTSSPA 182 +P S+I +AP + +A++ + Y +TI++EF HVHD +E WL+ ES Sbjct: 122 NIPISIISPDAPKHISNGIEAINHLRNTYKRTISFEFDHVHDFEERNWLSKSIESGELFK 181 Query: 183 PLNNAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDGAEH 242 + + RL +VEQFE FLHKTFVGQKRFS+EG DALVP+LDEI+ + G + Sbjct: 182 KKPADKLVSVFKRLTEVEQFEQFLHKTFVGQKRFSIEGLDALVPVLDEIISESVTQGTSN 241 Query: 243 ILMGMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYHLGA 302 I +GMAHRGRLNVLAH+LGKPY+IIFSEF H+PNKEL PSEGS+GI+YGWTGDVKYHLGA Sbjct: 242 INIGMAHRGRLNVLAHVLGKPYEIIFSEFQHAPNKELVPSEGSIGISYGWTGDVKYHLGA 301 Query: 303 DRAVREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAVLMH 362 DR +++ +T R+TLANNPSHLEF++P++EG RAAQE R G P D KA+A+L+H Sbjct: 302 DRQIKDEDTKSARVTLANNPSHLEFIDPIIEGSTRAAQELRTQKGYPAQDVEKALAILIH 361 Query: 363 GDAAFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAKGYE 422 GDAAFPGEGIVAETLN+ +L GY GGT+HII NN IGFTTES +SRST YASDLAKG+E Sbjct: 362 GDAAFPGEGIVAETLNLSQLVGYQVGGTIHIIANNMIGFTTESNESRSTKYASDLAKGFE 421 Query: 423 IPIVHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 IPIVHVNADDPEAC+AAV+LA YR +FKKDF+IDLIG Sbjct: 422 IPIVHVNADDPEACLAAVQLAVEYRKRFKKDFLIDLIG 459 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,850,309,198 Number of extensions: 118233963 Number of successful extensions: 274992 Number of sequences better than 1.0e-05: 1237 Number of HSP's gapped: 269727 Number of HSP's successfully gapped: 1240 Length of query: 460 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 321 Effective length of database: 1,751,175,118 Effective search space: 562127212878 Effective search space used: 562127212878 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)