BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0006.p (573 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K4C6.1 B3K4C6_9BACI Drug resistance transporter, EmrB/QacA sub... 688 0.0 C0Z7E1.1 C0Z7E1_BREBN Putative multidrug resistance protein 675 0.0 C3E4N2.1 C3E4N2_BACTU Drug resistance transporter, EmrB/QacA sub... 654 0.0 C2QD13.1 C2QD13_BACCE Drug resistance transporter, EmrB/QacA sub... 650 0.0 C2VV07.1 C2VV07_BACCE MFS family major facilitator transporter 631 e-179 >B3K4C6.1 B3K4C6_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 508 Score = 688 bits (1775), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/506 (65%), Positives = 414/506 (81%), Gaps = 7/506 (1%) Query: 68 MHSKESNXXXXXXXXXXXXXMSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAG 127 M++++SN M+A+DNTIVA+A+ TI+ +L G D++VWVTS YMV VMAG Sbjct: 1 MNAQQSNIKLVVAGLLLGIFMAAIDNTIVATALSTIIRDLQGFDQVVWVTSIYMVAVMAG 60 Query: 128 TPIFGKLSDMYGRKRFFIFGLIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFT 187 TPIFGKLSDMYGRKRFFIFGL+VFL GSALCG A ++T+L IYRAIQGIGGGALMPIAFT Sbjct: 61 TPIFGKLSDMYGRKRFFIFGLVVFLIGSALCGMANNMTELIIYRAIQGIGGGALMPIAFT 120 Query: 188 IMFDIYPPEKRGKLTGLFGAVFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILV 247 I+FD++PPEKRGK+TGL GAVFG SS++GPLLGA+ITD +GW+W+FY+N+PIG+++FI + Sbjct: 121 IVFDLFPPEKRGKMTGLLGAVFGTSSIMGPLLGAFITDSIGWEWIFYVNVPIGIVSFIFI 180 Query: 248 TLSYKESVTHSKQRIDWGGAFTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLI 307 SYKES +H+KQ+IDW GA TLVGA+ICLMF LELGG YAWDS QI+GLFGGF L I Sbjct: 181 MTSYKESRSHAKQKIDWTGAATLVGAIICLMFGLELGGQTYAWDSPQILGLFGGFIALFI 240 Query: 308 VFLFIERVAPEAVISFSMFRNRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNS 367 VFLF E A E +ISF MFR RLFA+S+I+ALFYG+ FIVAT+YIPI+V GVYGGSATNS Sbjct: 241 VFLFAETRAAEPIISFQMFRKRLFASSNIVALFYGATFIVATIYIPIFVSGVYGGSATNS 300 Query: 368 GLVLMPMMIGSVIGSQTGGLLTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMF 427 GL+LMPMM+GSV GSQ GG+LTT+TSFRNIM+LS +CF+ G+F LST++PDT R +L +F Sbjct: 301 GLILMPMMLGSVFGSQGGGMLTTRTSFRNIMILSVLCFITGIFCLSTISPDTPRYLLTLF 360 Query: 428 MALTGFGVGFSFSVLSMSSIHHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTN 487 M LTGFGVGFSFS LSM+SIH+FD RQRG+ATST++F+RS GMT+GITIFGIIQRN N Sbjct: 361 MVLTGFGVGFSFSTLSMASIHNFDARQRGAATSTNSFLRSFGMTVGITIFGIIQRNGLNN 420 Query: 488 GLSDAFG---AGADTSSFG----DPRAALTPEARAQIPAPVLEKITNALSGSIAHTFLWA 540 L++A G AGA+ ++ + A L+PE Q+P V E+IT +L+ SIA+TF+WA Sbjct: 421 RLAEAGGNAPAGAELNAPAGAELNADAVLSPEVMGQLPPNVREQITASLADSIANTFMWA 480 Query: 541 LVPAVLGFVFVLLMPGDRLTVRSKKQ 566 LVPAVL VFVL+M +R+TV +K + Sbjct: 481 LVPAVLALVFVLMMGNERVTVPAKAK 506 >C0Z7E1.1 C0Z7E1_BREBN Putative multidrug resistance protein Length = 505 Score = 675 bits (1742), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/505 (66%), Positives = 411/505 (81%), Gaps = 1/505 (0%) Query: 68 MHSKESNXXXXXXXXXXXXXMSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAG 127 M ++ES M++MDNTIVA+AMGTIV E+GG+DK VWVTSAYMV MAG Sbjct: 1 MIARESKVNMVLAGLLLGLLMASMDNTIVATAMGTIVGEMGGMDKFVWVTSAYMVATMAG 60 Query: 128 TPIFGKLSDMYGRKRFFIFGLIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFT 187 PIFGKLSDMYGRKRF++FGLIVFL GS LCGTA SI +LSIYRAIQGIGGGALMPIAFT Sbjct: 61 MPIFGKLSDMYGRKRFYVFGLIVFLLGSILCGTANSIVELSIYRAIQGIGGGALMPIAFT 120 Query: 188 IMFDIYPPEKRGKLTGLFGAVFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILV 247 I+FD++PPEKRGK+TGLFGAVFG SSV+GPLLGAYIT+YV W W+FYIN+PIG ++ L+ Sbjct: 121 IIFDVFPPEKRGKMTGLFGAVFGTSSVLGPLLGAYITEYVSWHWIFYINVPIGAVSLWLI 180 Query: 248 TLSYKESVTHSKQRIDWGGAFTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLI 307 ++ YKES+ + KQ IDW GA TLVGAV+ LMFALELGGNQYAW+S QI+GLF F +L + Sbjct: 181 SMYYKESLEYRKQSIDWWGAITLVGAVVSLMFALELGGNQYAWNSMQIIGLFSSFFLLFV 240 Query: 308 VFLFIERVAPEAVISFSMFRNRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNS 367 +FLF+E+ A + +ISFSMF+ RLFAT++ +AL YG+AFI++TVY+PI++QGVYGGSATN+ Sbjct: 241 IFLFVEKRASDPIISFSMFKKRLFATTNAVALLYGAAFIISTVYLPIFIQGVYGGSATNA 300 Query: 368 GLVLMPMMIGSVIGSQTGGLLTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMF 427 GL+L PMM+GSV GSQ GG LTTKTSFRNIMLLSAV F+ G+F LSTL P+ SR ++ ++ Sbjct: 301 GLLLTPMMLGSVAGSQLGGFLTTKTSFRNIMLLSAVFFIPGIFLLSTLTPEISRSIVTLY 360 Query: 428 MALTGFGVGFSFSVLSMSSIHHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTN 487 M + GFGVGFSFS L +S+IH FDMRQRGSA+ST++F RSLGMTLGIT+FGI+QRNSF N Sbjct: 361 MVIAGFGVGFSFSTLGISAIHGFDMRQRGSASSTNSFSRSLGMTLGITVFGILQRNSFEN 420 Query: 488 GLSDAFGAGADTSSFGDPRAALTPEARAQIPAPVLEKITNALSGSIAHTFLWALVPAVLG 547 GL AFG T++ DPRA LTPEAR IPAP+L+K+ + L+ SIA+TF+WALVPAVL Sbjct: 421 GLFSAFGGQGMTNAITDPRAILTPEARESIPAPILDKMIDILASSIANTFMWALVPAVLA 480 Query: 548 FVFVLLMPGDRLTVRSKKQ-QPADG 571 V +LLM DR +S +P G Sbjct: 481 LVCILLMGNDRALGKSPTAPKPERG 505 >C3E4N2.1 C3E4N2_BACTU Drug resistance transporter, EmrB/QacA subfamily Length = 511 Score = 654 bits (1688), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/483 (67%), Positives = 397/483 (82%), Gaps = 5/483 (1%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 M++MDNTIV +AMGTIV +LGGL+ VWV SAYMV MAG PIFGKLSDMYGRKRFFIFG Sbjct: 21 MASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG 80 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 LIVF+ GSALCGTA +ITQL IYRAIQGIGGGAL+PIAFTI+FDI+PPEKRGK+ GLFGA Sbjct: 81 LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGA 140 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKESVTHSKQRIDWGGA 267 VFG+SS+ GPLLGAYITDY+ W WVFYINLP+G++A I +T YKES H KQ+IDW GA Sbjct: 141 VFGLSSIFGPLLGAYITDYISWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWFGA 200 Query: 268 FTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFSMFR 327 TLVGAVICLMFALELGG +Y WDS+ I+ LFGGFA+L+I F+FIER E +ISF MF+ Sbjct: 201 ITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILIIAFIFIERKVEEPIISFEMFK 260 Query: 328 NRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTGGL 387 RLF S+I+AL YG+AF+ ATVYIP+++QGVYGG+ATNSGL+L+PMM+GSV+ +Q GG Sbjct: 261 QRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMVGSVVTAQLGGF 320 Query: 388 LTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMSSI 447 LTTK S+RNIM++SAV + G+F LSTL P+TSR +L ++M + GFGVGFSFSVLSM++I Sbjct: 321 LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAI 380 Query: 448 HHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAF---GAGADTSSFGD 504 H+F M QRGSATSTS F+RSLGMTLGITIFG+IQR F + L +AF G +T++ GD Sbjct: 381 HNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGD 440 Query: 505 PRAALTPEARAQIPAPVLEKITNALSGSIAHTFLWALVPAVLGFVFVLLMPGDRLTVRSK 564 RA L+ AR+QIP +L+KI +ALS SI TF+WALVPA L F+F+ M +R+ + K Sbjct: 441 SRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPAGLAFIFIFFMGNERMVI--K 498 Query: 565 KQQ 567 KQQ Sbjct: 499 KQQ 501 >C2QD13.1 C2QD13_BACCE Drug resistance transporter, EmrB/QacA subfamily Length = 511 Score = 650 bits (1677), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/481 (67%), Positives = 395/481 (82%), Gaps = 3/481 (0%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 M++MDNTIV +AMGTIV +LGGL+ VWV SAYMV MAG PIFGKLSDMYGRKRFFIFG Sbjct: 21 MASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG 80 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 LIVF+ GSALCGTA +ITQL IYRAIQGIGGGAL+PIAFTI+FDI+PPEKRGK+ GLFGA Sbjct: 81 LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGA 140 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKESVTHSKQRIDWGGA 267 VFG+SS+ GPLLGAYITDY+ W WVFYINLP+G++A I +T YKES H KQ+IDW GA Sbjct: 141 VFGLSSIFGPLLGAYITDYISWHWVFYINLPLGILALIFITFFYKESRVHRKQKIDWLGA 200 Query: 268 FTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFSMFR 327 TLVGAV+CLMFALELGG +Y WDS+ I+ LFGGFA+L+I F+FIER E +ISF MF+ Sbjct: 201 ITLVGAVVCLMFALELGGQKYDWDSSFILSLFGGFAILVIAFIFIERKVEEPIISFEMFK 260 Query: 328 NRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTGGL 387 RLF S+I+AL YG+AF+ ATVYIP+++QGVYGG+ATNSGL+L+PMM+GSV+ +Q GG Sbjct: 261 QRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGTATNSGLLLLPMMLGSVVTAQLGGF 320 Query: 388 LTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMSSI 447 LTTK S+RNIM +SAV + G+F LSTL P+TSR +L ++M + GFGVGFSFSVLSM+SI Sbjct: 321 LTTKLSYRNIMFISAVIMLIGLFLLSTLTPETSRALLTIYMIIIGFGVGFSFSVLSMASI 380 Query: 448 HHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAF---GAGADTSSFGD 504 H+F M QRGSATSTS F+RSLGMTLGITIFG+IQR F + L +AF G +T++ GD Sbjct: 381 HNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGD 440 Query: 505 PRAALTPEARAQIPAPVLEKITNALSGSIAHTFLWALVPAVLGFVFVLLMPGDRLTVRSK 564 RA L+ AR+QIP +L+KI +ALS SI TF+WALVPA L F+F+ M +R+ ++ K Sbjct: 441 SRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPAGLAFIFIFFMGNERMVIKKK 500 Query: 565 K 565 + Sbjct: 501 Q 501 >C2VV07.1 C2VV07_BACCE MFS family major facilitator transporter Length = 511 Score = 631 bits (1627), Expect = e-179, Method: Compositional matrix adjust. Identities = 326/488 (66%), Positives = 399/488 (81%), Gaps = 4/488 (0%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 M++MDNTIV +AMGTIV +LGGL+ VWV SAYMV MAG PIFGKLSDMYGRKRFFIFG Sbjct: 21 MASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG 80 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 LIVF+ GSALCGTA +ITQL IYRAIQGIGGGAL+PIAFTI+FDI+PPEKRGK+ GLFGA Sbjct: 81 LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGA 140 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKESVTHSKQRIDWGGA 267 VFG+SS+ GPLLGAYITDY+ W WVFYINLP+GV+A I +T YKES H KQ+IDW GA Sbjct: 141 VFGLSSIFGPLLGAYITDYISWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGA 200 Query: 268 FTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFSMFR 327 TLVGAVICLMFALELGG +Y WDS+ I+ LFGGFA+L+I F+FIER E +ISF MF+ Sbjct: 201 ITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILIIAFIFIERKVEEPIISFEMFK 260 Query: 328 NRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTGGL 387 RLF S+I+AL YG+AF+ ATVYIP+++QGVYGGSATNSGL+L+PMM+GSV+ +Q GG Sbjct: 261 QRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF 320 Query: 388 LTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMSSI 447 LTTK S+RNIM++SAV + G+F LSTL P+TSR +L ++M + GFGVGFSFSVLSM++I Sbjct: 321 LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAI 380 Query: 448 HHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAF---GAGADTSSFGD 504 H+F M QRGSATSTS F+RSLGMTLGITIFG+IQR F + L + F G +T++ GD Sbjct: 381 HNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQDQLEEVFKGMSGGMNTNALGD 440 Query: 505 PRAALTPEARAQIPAPVLEKITNALSGSIAHTFLWALVPAVLGFVFVLLMPGDRLTVRSK 564 RA L+ AR+QIP +L+KI +ALS SI TF+WALVPA L F+F+ M +R+ ++ K Sbjct: 441 SRAILSESARSQIPPQILDKIIDALSSSIVQTFMWALVPAGLAFIFIFFMGNERMVMK-K 499 Query: 565 KQQPADGQ 572 +Q+ G+ Sbjct: 500 QQKNKKGE 507 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,101,906,027 Number of extensions: 123676252 Number of successful extensions: 602886 Number of sequences better than 1.0e-05: 16717 Number of HSP's gapped: 583076 Number of HSP's successfully gapped: 16885 Length of query: 573 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 432 Effective length of database: 1,732,333,088 Effective search space: 748367894016 Effective search space used: 748367894016 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 134 (56.2 bits)