BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0206.0002.p (234 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGD6.1 B3KGD6_9BACI Uracil-DNA glycosylase EC=3.2.2.- 325 2e-87 C0Z887.1 UNG_BREBN Uracil-DNA glycosylase EC=3.2.2.- 306 1e-81 Q65DN9.1 UNG_BACLD Uracil-DNA glycosylase EC=3.2.2.- 300 6e-80 A7ZA24.1 UNG_BACA2 Uracil-DNA glycosylase EC=3.2.2.- 295 2e-78 P39615.1 UNG_BACSU Uracil-DNA glycosylase EC=3.2.2.- 293 1e-77 >B3KGD6.1 B3KGD6_9BACI Uracil-DNA glycosylase EC=3.2.2.- Length = 230 Score = 325 bits (832), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 149/221 (67%), Positives = 180/221 (81%) Query: 13 MFGNDWDDILQEETQKPYFQELRYALAREYKQYTVYPPKEELFSALKLTPYNSTRVVILG 72 MF NDWD ILQ E KPYFQ L + EY++ TVYPPKE++F AL+ T Y ST+VVILG Sbjct: 3 MFNNDWDLILQGEVDKPYFQALMERVDEEYRRSTVYPPKEDIFRALQQTSYQSTKVVILG 62 Query: 73 QDPYHGAGQAHGLSFSVKPGVRVPPSLRNIYTELHDDMGVSIPNHGSLLHWAEEGVLLLN 132 QDPYHG QA GLSFSV PGV +PPSLRNI+ EL D+G+S PNHGSL WA++GVLLLN Sbjct: 63 QDPYHGRRQAQGLSFSVSPGVAIPPSLRNIHKELSSDLGISAPNHGSLQSWADQGVLLLN 122 Query: 133 AVLTVREGQPNSHKGLGWETFTDAVMEKLNERKAPLVFILWGSHAQQKGAYIDRSRHLVI 192 AVLTVREG+PNSHKG+GWETFTD V+ KLNER+ P+VFILWGS+AQ+KG++IDRSRH V+ Sbjct: 123 AVLTVREGEPNSHKGIGWETFTDKVIAKLNERERPMVFILWGSYAQKKGSFIDRSRHKVL 182 Query: 193 QSPHPSPFSAHRGFLGSRPFSRANQYLESHGLQGIDWSIPN 233 +S HPSPF+AH+GF GSRPFS +N +L G+ ++W IPN Sbjct: 183 ESSHPSPFAAHKGFFGSRPFSASNAFLMQEGMDPVNWDIPN 223 >C0Z887.1 UNG_BREBN Uracil-DNA glycosylase EC=3.2.2.- Length = 229 Score = 306 bits (783), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 139/222 (62%), Positives = 171/222 (77%) Query: 11 TEMFGNDWDDILQEETQKPYFQELRYALAREYKQYTVYPPKEELFSALKLTPYNSTRVVI 70 T + NDW +L +E +KPY+ +LR L EY+ T+YP +F+AL LT Y + +VVI Sbjct: 2 TTILQNDWAPVLADEFEKPYYVKLRQTLKEEYQTQTIYPDMFHIFTALHLTEYQNAKVVI 61 Query: 71 LGQDPYHGAGQAHGLSFSVKPGVRVPPSLRNIYTELHDDMGVSIPNHGSLLHWAEEGVLL 130 LGQDPYHG GQAHGLSFSVKPG++ PPSL NIY EL D+G IP HG L HWA++GV++ Sbjct: 62 LGQDPYHGPGQAHGLSFSVKPGIKPPPSLVNIYKELKSDVGFEIPQHGYLNHWAKQGVMM 121 Query: 131 LNAVLTVREGQPNSHKGLGWETFTDAVMEKLNERKAPLVFILWGSHAQQKGAYIDRSRHL 190 LN VLTVR G PNSHK +GWETFTD ++ LN+R+ PLVFILWG HAQ+K A+IDR++H Sbjct: 122 LNTVLTVRRGTPNSHKDIGWETFTDRIIHLLNDRETPLVFILWGKHAQEKAAFIDRNKHF 181 Query: 191 VIQSPHPSPFSAHRGFLGSRPFSRANQYLESHGLQGIDWSIP 232 VI SPHPSPFSA+RGF GSRPFSR N++L S GLQ IDW +P Sbjct: 182 VIASPHPSPFSANRGFFGSRPFSRTNEFLRSRGLQEIDWQLP 223 >Q65DN9.1 UNG_BACLD Uracil-DNA glycosylase EC=3.2.2.- Length = 225 Score = 300 bits (768), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 139/223 (62%), Positives = 172/223 (77%) Query: 12 EMFGNDWDDILQEETQKPYFQELRYALAREYKQYTVYPPKEELFSALKLTPYNSTRVVIL 71 ++ + W L++E KPY+QELR L REY ++TVYP ++++AL T Y + +VVIL Sbjct: 3 QILNDSWWQQLKDEFDKPYYQELREMLKREYAEHTVYPEPNDIYNALHYTSYENVKVVIL 62 Query: 72 GQDPYHGAGQAHGLSFSVKPGVRVPPSLRNIYTELHDDMGVSIPNHGSLLHWAEEGVLLL 131 GQDPYHG GQAHGLSFSV+PGV PPSL+NI+ EL +D+G IPNHGSL+ WA++GVLLL Sbjct: 63 GQDPYHGPGQAHGLSFSVQPGVNPPPSLKNIFIELQNDIGADIPNHGSLVSWAKQGVLLL 122 Query: 132 NAVLTVREGQPNSHKGLGWETFTDAVMEKLNERKAPLVFILWGSHAQQKGAYIDRSRHLV 191 N VLTVR GQ NSHKG GWE TD++++ LN+R P+VFILWG HAQ K ID S+H + Sbjct: 123 NTVLTVRRGQANSHKGKGWEQLTDSIIDVLNKRDKPVVFILWGRHAQMKKERIDTSKHFI 182 Query: 192 IQSPHPSPFSAHRGFLGSRPFSRANQYLESHGLQGIDWSIPNI 234 IQSPHPSPFSA GF GSRPFSRANQYLE G + IDWS+PN+ Sbjct: 183 IQSPHPSPFSARNGFFGSRPFSRANQYLEQIGDEPIDWSLPNL 225 >A7ZA24.1 UNG_BACA2 Uracil-DNA glycosylase EC=3.2.2.- Length = 227 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 135/223 (60%), Positives = 168/223 (75%) Query: 12 EMFGNDWDDILQEETQKPYFQELRYALAREYKQYTVYPPKEELFSALKLTPYNSTRVVIL 71 ++ + W + L++E KPY+QELR L +EY T+YP ++F+AL TPY + VIL Sbjct: 3 QLLKDSWWNQLKDEFDKPYYQELREMLKQEYSSQTIYPDSHDIFNALHYTPYEDVKAVIL 62 Query: 72 GQDPYHGAGQAHGLSFSVKPGVRVPPSLRNIYTELHDDMGVSIPNHGSLLHWAEEGVLLL 131 GQDPYHG GQAHGLSFSV+PGVR PPSL+NI+ EL DD+G S+PNHGSL+ WA++GVLLL Sbjct: 63 GQDPYHGPGQAHGLSFSVQPGVRQPPSLKNIFIELQDDIGCSVPNHGSLVSWAKQGVLLL 122 Query: 132 NAVLTVREGQPNSHKGLGWETFTDAVMEKLNERKAPLVFILWGSHAQQKGAYIDRSRHLV 191 N VLTVR GQ NSHKG GWE TD +++ +NER+ P+VFILWG HAQ K ID S+H++ Sbjct: 123 NTVLTVRRGQANSHKGKGWERLTDRIIDVVNEREKPIVFILWGRHAQMKKERIDTSKHII 182 Query: 192 IQSPHPSPFSAHRGFLGSRPFSRANQYLESHGLQGIDWSIPNI 234 I+S HPSPFSA GF GSRPFSR N LE G Q IDW IP++ Sbjct: 183 IESTHPSPFSARNGFFGSRPFSRTNAALEKMGEQPIDWCIPDL 225 >P39615.1 UNG_BACSU Uracil-DNA glycosylase EC=3.2.2.- Length = 225 Score = 293 bits (749), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 135/223 (60%), Positives = 168/223 (75%) Query: 12 EMFGNDWDDILQEETQKPYFQELRYALAREYKQYTVYPPKEELFSALKLTPYNSTRVVIL 71 ++ + W + L+EE +KPY+QELR L REY + T+YP ++F+AL T Y+ +VVIL Sbjct: 3 QLLQDSWWNQLKEEFEKPYYQELREMLKREYAEQTIYPDSRDIFNALHYTSYDDVKVVIL 62 Query: 72 GQDPYHGAGQAHGLSFSVKPGVRVPPSLRNIYTELHDDMGVSIPNHGSLLHWAEEGVLLL 131 GQDPYHG GQA GLSFSVKPGV+ PPSL+NI+ EL D+G SIPNHGSL+ WA++GVLLL Sbjct: 63 GQDPYHGPGQAQGLSFSVKPGVKQPPSLKNIFLELQQDIGCSIPNHGSLVSWAKQGVLLL 122 Query: 132 NAVLTVREGQPNSHKGLGWETFTDAVMEKLNERKAPLVFILWGSHAQQKGAYIDRSRHLV 191 N VLTVR GQ NSHKG GWE TD +++ L+ER+ P++FILWG HAQ K ID S+H + Sbjct: 123 NTVLTVRRGQANSHKGKGWERLTDRIIDVLSERERPVIFILWGRHAQMKKERIDTSKHFI 182 Query: 192 IQSPHPSPFSAHRGFLGSRPFSRANQYLESHGLQGIDWSIPNI 234 I+S HPSPFSA GF GSRPFSRAN YLE G IDW I ++ Sbjct: 183 IESTHPSPFSARNGFFGSRPFSRANAYLEKMGEAPIDWCIKDL 225 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,560,354,653 Number of extensions: 65353109 Number of successful extensions: 137120 Number of sequences better than 1.0e-05: 1416 Number of HSP's gapped: 135201 Number of HSP's successfully gapped: 1417 Length of query: 234 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 103 Effective length of database: 1,826,543,238 Effective search space: 188133953514 Effective search space used: 188133953514 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)