BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0205.0004.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DD39.1 B1DD39_9BACL Binding-protein-dependent transport system... 297 1e-78 C1PZW9.1 C1PZW9_9FIRM Carbohydrate ABC transporter membrane prot... 293 2e-77 B1DGM8.1 B1DGM8_9BACL Binding-protein-dependent transport system... 281 6e-74 B3KGE4.1 B3KGE4_9BACI Binding-protein-dependent transport system... 280 1e-73 Q9KEQ4.1 Q9KEQ4_BACHD Sugar transport system (Permease) (Binding... 274 7e-72 >B1DD39.1 B1DD39_9BACL Binding-protein-dependent transport systems inner membrane component Length = 311 Score = 297 bits (760), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 143/276 (51%), Positives = 203/276 (73%), Gaps = 10/276 (3%) Query: 31 PIVLVFMISITDYDSIVLNGYQFIPEKLSLEAYAYIFKDYAVIVKAYGVSIFVTVAGTLL 90 P++L+ +S +D S++ +GY+FIPEK +L AYA+++KD I +YG+SI VTV GT L Sbjct: 40 PLLLLLSVSFSDEKSVLTHGYRFIPEKFNLAAYAFLYKDIGQIAYSYGISITVTVVGTAL 99 Query: 91 SVFISSLYAYPISRADFKYKGXXXXXXXXXXXXGGGLVPWYMVYTQVLDLKNSIWALIVP 150 S+ I +LYAYPISR+ F + GGLVPWY+VY Q+L+LK+++WALI+P Sbjct: 100 SMLIIALYAYPISRSQFPHARFFTFFVFFTMLFSGGLVPWYLVYVQMLNLKDTLWALIMP 159 Query: 151 MLLSPFNVLIMKTYFQMSVPPALIEASKIDGAGELRTFFRIVFPLSLPVFATIGLFNTLH 210 +L+S F VLI++T+F+ ++P A++E++KIDGAGELR F RIV PLS+PV AT+ LF TL Sbjct: 160 LLVSSFWVLIVRTFFKETIPAAVLESAKIDGAGELRIFIRIVLPLSMPVLATVALFQTLT 219 Query: 211 YWNDWFNSMIFITDTDLYSLQYLMYKMISQADYLSRNGALIQGSATELAK------LPGE 264 YWNDWF S++FIT+ S+QYL+YKM++ YLS N +A E+A+ P E Sbjct: 220 YWNDWFLSLVFITNDHNISVQYLLYKMLANIQYLSSNPT----AAAEIARAGGMFNFPSE 275 Query: 265 TIRMAMALIGIGPIVLAYPFFQRYFIKGLTLGSIKG 300 T+RMA+ ++GIGPIV AYPFFQ+YF++GLT+G++KG Sbjct: 276 TVRMALVVVGIGPIVFAYPFFQKYFVRGLTVGAVKG 311 >C1PZW9.1 C1PZW9_9FIRM Carbohydrate ABC transporter membrane protein 2, CUT1 family Length = 307 Score = 293 bits (749), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 143/293 (48%), Positives = 197/293 (67%), Gaps = 1/293 (0%) Query: 8 EISRPTNXXXXXXXXXXXXXXXXPIVLVFMISITDYDSIVLNGYQFIPEKLSLEAYAYIF 67 E+S+ N P + VF+ISITD ++ +NGY PEK+S +AY IF Sbjct: 14 EVSKGANIGLNIYLILLTISAILPFLFVFIISITDESTLAINGYSLFPEKISFDAYKLIF 73 Query: 68 KDYAVIVKAYGVSIFVTVAGTLLSVFISSLYAYPISRADFKYKGXXXXXXXXXXXXGGGL 127 + IV+AY VSIF+TV G +L++ +++LYAY +SR DF++KG GGL Sbjct: 74 SSGSSIVQAYKVSIFITVVGVILALILTTLYAYALSRDDFEFKGFFTKVSTIPMLFSGGL 133 Query: 128 VPWYMVYTQVLDLKNSIWALIVPMLLSPFNVLIMKTYFQMSVPPALIEASKIDGAGELRT 187 V Y+V TQ L L+NS+WALI+P+LL FN++IM+T+FQ S+P ALIEA+ IDGA E + Sbjct: 134 VASYLVMTQFLGLRNSVWALILPLLLGTFNIIIMRTFFQTSIPKALIEAATIDGANEFQI 193 Query: 188 FFRIVFPLSLPVFATIGLFNTLHYWNDWFNSMIFITDTDLYSLQYLMYKMISQADYLSRN 247 FF++V PLSLPV ATIGLF TL YWNDW+N+M++I D D +QYL+ ++ + +L+ Sbjct: 194 FFKVVLPLSLPVLATIGLFLTLGYWNDWYNAMLYIDDLDKIPVQYLLIRIQNSTAFLASR 253 Query: 248 GALIQGSATELAK-LPGETIRMAMALIGIGPIVLAYPFFQRYFIKGLTLGSIK 299 G+ I G A + K LP ET+RMA+ +I PI + YPFFQRYFI GLTLG++K Sbjct: 254 GSQIGGVANQAMKTLPQETLRMAIVVITTAPIAITYPFFQRYFISGLTLGAVK 306 >B1DGM8.1 B1DGM8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 281 bits (718), Expect = 6e-74, Method: Compositional matrix adjust. Identities = 131/271 (48%), Positives = 194/271 (71%), Gaps = 3/271 (1%) Query: 31 PIVLVFMISITDYDSIVLNGYQFIPEKLSLEAYAYIFKDYAVIVKAYGVSIFVTVAGTLL 90 P +L+FM SITD +IV GY P S EAY Y++K + I AYGV++ VTV GTL Sbjct: 26 PFILLFMASITDESTIVKEGYTLFPSAFSFEAYEYLWKQSSQIFHAYGVTVLVTVIGTLA 85 Query: 91 SVFISSLYAYPISRADFKYKGXXXXXXXXXXXXGGGLVPWYMVYTQVLDLKNSIWALIVP 150 S+ I+SL AYP+SR D GGLVP Y++YTQV LKN+++AL++P Sbjct: 86 SLIITSLLAYPLSRKDLPGGQTIAFIVFFTLLFNGGLVPTYLIYTQVFHLKNTLFALLIP 145 Query: 151 -MLLSPFNVLIMKTYFQMSVPPALIEASKIDGAGELRTFFRIVFPLSLPVFATIGLFNTL 209 +L++ FNVL+M+T+F ++P A++E++ IDGAGE +T+FRI+ PLS+P+ AT+G+F L Sbjct: 146 WLLMNGFNVLLMRTFFSTTIPTAMLESASIDGAGEFKTYFRIILPLSMPILATVGMFQAL 205 Query: 210 HYWNDWFNSMIFITDTDLYSLQYLMYKMISQADYLSRNGALIQGSATELAKLPGETIRMA 269 YWNDW N ++F+T+ DL+SLQ ++ +++S +L+ N + G++ LA+LP ET RMA Sbjct: 206 GYWNDWNNGLVFVTNPDLFSLQNVLNRIMSDIQFLTTNSRM--GTSQALAQLPSETFRMA 263 Query: 270 MALIGIGPIVLAYPFFQRYFIKGLTLGSIKG 300 +A+IG+ PI+L YPFFQ+YF+KG+T+G++KG Sbjct: 264 IAVIGVIPILLVYPFFQKYFVKGMTIGAVKG 294 >B3KGE4.1 B3KGE4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 305 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 136/269 (50%), Positives = 190/269 (70%) Query: 31 PIVLVFMISITDYDSIVLNGYQFIPEKLSLEAYAYIFKDYAVIVKAYGVSIFVTVAGTLL 90 P + V +IS TD S+ NGYQ PEK SLEAY Y+F+ ++++YGV+IFVTV GTLL Sbjct: 36 PFLFVMIISFTDEGSLARNGYQIWPEKWSLEAYKYVFQTGDTLLRSYGVTIFVTVVGTLL 95 Query: 91 SVFISSLYAYPISRADFKYKGXXXXXXXXXXXXGGGLVPWYMVYTQVLDLKNSIWALIVP 150 S+FI SLYAY ISR F+Y+ GGLVP Y+V TQ+L LK+SIWALI+P Sbjct: 96 SLFIISLYAYAISRKGFRYRNFFSFFAFFTMLFNGGLVPTYIVATQLLGLKDSIWALILP 155 Query: 151 MLLSPFNVLIMKTYFQMSVPPALIEASKIDGAGELRTFFRIVFPLSLPVFATIGLFNTLH 210 + L+ F ++I++T++ SVP A+IE+ KIDGA E + F+++V PLSLP ATIGLF+TL Sbjct: 156 LALNAFYIMILRTFYSTSVPDAIIESGKIDGASEFKIFYKLVLPLSLPGLATIGLFSTLG 215 Query: 211 YWNDWFNSMIFITDTDLYSLQYLMYKMISQADYLSRNGALIQGSATELAKLPGETIRMAM 270 YWNDWFN++++I D +L LQ ++ ++ + ++ +N S L +P +T RMAM Sbjct: 216 YWNDWFNALLYIDDPNLVPLQSMLMRIETSMQFILQNSQNSSLSMAALQSMPQDTSRMAM 275 Query: 271 ALIGIGPIVLAYPFFQRYFIKGLTLGSIK 299 ++ GPI+LAYPFFQRYFI+GLT+G++K Sbjct: 276 VVLATGPIILAYPFFQRYFIQGLTIGAVK 304 >Q9KEQ4.1 Q9KEQ4_BACHD Sugar transport system (Permease) (Binding protein dependent transporter) Length = 322 Score = 274 bits (701), Expect = 7e-72, Method: Compositional matrix adjust. Identities = 130/269 (48%), Positives = 189/269 (70%) Query: 31 PIVLVFMISITDYDSIVLNGYQFIPEKLSLEAYAYIFKDYAVIVKAYGVSIFVTVAGTLL 90 P + V +IS+T S+ +NGY+ IP + S +AY Y+++ + + +AYGV+IF+TV GTL+ Sbjct: 53 PFIYVIIISLTSEQSLAMNGYRIIPSEWSFDAYRYLWQMRSEVFRAYGVTIFITVVGTLM 112 Query: 91 SVFISSLYAYPISRADFKYKGXXXXXXXXXXXXGGGLVPWYMVYTQVLDLKNSIWALIVP 150 SV I +LYAY ISR F Y+ GG+VP+Y+V TQ L LKNS+WALI+P Sbjct: 113 SVSIIALYAYAISRKQFLYRKLFTFIAFFTMLFSGGMVPFYIVMTQFLGLKNSVWALILP 172 Query: 151 MLLSPFNVLIMKTYFQMSVPPALIEASKIDGAGELRTFFRIVFPLSLPVFATIGLFNTLH 210 M ++ F ++IM+T+FQ SVP A++E+++IDGAGE R FF+IV PLS+P ATI LF+TL Sbjct: 173 MAVNAFYIIIMRTFFQRSVPEAILESARIDGAGEFRIFFKIVLPLSIPGIATIALFSTLA 232 Query: 211 YWNDWFNSMIFITDTDLYSLQYLMYKMISQADYLSRNGALIQGSATELAKLPGETIRMAM 270 YWNDWFN+++FI D L LQ L+ ++ + D++ +N L ++ L +P ++ RMAM Sbjct: 233 YWNDWFNALLFIDDPKLVPLQSLLMRIENNLDFIRQNAMLSSQNSALLDSIPQDSARMAM 292 Query: 271 ALIGIGPIVLAYPFFQRYFIKGLTLGSIK 299 +I PI L+YPFFQ+YF+KGLT+G +K Sbjct: 293 VVIATLPIALSYPFFQKYFVKGLTIGGVK 321 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.142 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,504,808,853 Number of extensions: 54175889 Number of successful extensions: 196876 Number of sequences better than 1.0e-05: 4779 Number of HSP's gapped: 191561 Number of HSP's successfully gapped: 4782 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)