BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0204.0010.p (268 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8TKE9.1 Q8TKE9_METAC Nickel ABC transporter, permease protein 306 1e-81 B1I1X8.1 B1I1X8_DESAP Binding-protein-dependent transport system... 304 7e-81 C1TBN9.1 C1TBN9_9FIRM Nickel ABC transporter, permease subunit NikC 303 1e-80 Q46F90.1 Q46F90_METBF Nickel ABC transporter, permease protein 300 7e-80 C0CI01.1 C0CI01_9FIRM Putative uncharacterized protein 292 2e-77 >Q8TKE9.1 Q8TKE9_METAC Nickel ABC transporter, permease protein Length = 289 Score = 306 bits (783), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 140/256 (54%), Positives = 198/256 (77%) Query: 10 GGMIVFLFVCIGIFAPWLAPSDPLQIHMEHKLSMPSWEYPLGTDHLGRCVLSRLIYGTRT 69 G +IV L + + I A +AP+DPL+++M + PS Y LGTD+LGRC+ SRLI+G R Sbjct: 34 GLLIVALIIFVAIAASVIAPNDPLKVNMSMRFKGPSSLYWLGTDNLGRCIASRLIWGARM 93 Query: 70 SLYYSLMVLAAVFSISIPVGLLAGYVGGIIDHLIMRVIDILLAFPSLILSLAVTAVLGPS 129 SL YSL VL + +IS P+GLL+GY GG +D IMRV DI L+ P+ +++LA+ +LGPS Sbjct: 94 SLIYSLCVLGLMMAISTPIGLLSGYAGGRMDTFIMRVTDIFLSLPTFLMALAIAGMLGPS 153 Query: 130 MKNLLISFAAVWWAGYARVIRSMVLQIKESDYIMAAKAGGSTHLQIILRHILLNATRPIL 189 +KN++I+ A+VWWA YAR+IR M +QIK+ D+++AAKA TH QII RHIL N PI+ Sbjct: 154 VKNMIIAMASVWWAPYARIIRGMAMQIKQQDFMLAAKAAACTHCQIIFRHILRNIASPII 213 Query: 190 VLASIEIGTLMLSIAGLSFLGLGAQPPTPEWGVMLNDSRPYIQTEPRLLLYPGLAIMLSV 249 V+A++EIG+++L+IA SF+GLGAQPPTPEWG+ML+DS+ ++QT+P+L++YPG+AI+ +V Sbjct: 214 VMATLEIGSIILAIASFSFIGLGAQPPTPEWGIMLSDSKEFMQTQPQLMIYPGIAILFTV 273 Query: 250 LGFNLLGEGLRKPGQK 265 + FNLLGEGL+ Q Sbjct: 274 MAFNLLGEGLKNAIQD 289 >B1I1X8.1 B1I1X8_DESAP Binding-protein-dependent transport systems inner membrane component Length = 309 Score = 304 bits (778), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 141/239 (58%), Positives = 191/239 (79%) Query: 23 FAPWLAPSDPLQIHMEHKLSMPSWEYPLGTDHLGRCVLSRLIYGTRTSLYYSLMVLAAVF 82 FAP++AP+DP+++ + +L+ P +YPLGTDHLGRC+LSRLIYGTR SL + + L A+ Sbjct: 61 FAPYIAPNDPVKVDLAQRLAPPGAQYPLGTDHLGRCLLSRLIYGTRVSLATAALALTAIM 120 Query: 83 SISIPVGLLAGYVGGIIDHLIMRVIDILLAFPSLILSLAVTAVLGPSMKNLLISFAAVWW 142 ISIP+G LAGY GG +D+ +MRV+D+LLAFP L+L+L + +LGP + N++++ AAVWW Sbjct: 121 LISIPLGTLAGYCGGRVDNAVMRVVDVLLAFPGLVLALVIAGMLGPGLLNVMLALAAVWW 180 Query: 143 AGYARVIRSMVLQIKESDYIMAAKAGGSTHLQIILRHILLNATRPILVLASIEIGTLMLS 202 GYARVIR MVL +KE ++++AA+A G+ L II RHIL N P++VLA++++G L+L+ Sbjct: 181 VGYARVIRGMVLSVKEKEFVLAARACGTRELGIITRHILPNVLSPVIVLATLDMGKLILA 240 Query: 203 IAGLSFLGLGAQPPTPEWGVMLNDSRPYIQTEPRLLLYPGLAIMLSVLGFNLLGEGLRK 261 I+GLSFLGLGAQPPTPEWG MLND RPY+Q P+L++YPGL IM VL FNLLG+GLR Sbjct: 241 ISGLSFLGLGAQPPTPEWGAMLNDGRPYMQVAPQLMVYPGLCIMTVVLAFNLLGDGLRD 299 >C1TBN9.1 C1TBN9_9FIRM Nickel ABC transporter, permease subunit NikC Length = 284 Score = 303 bits (776), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 141/261 (54%), Positives = 198/261 (75%) Query: 1 MFKSNGAKAGGMIVFLFVCIGIFAPWLAPSDPLQIHMEHKLSMPSWEYPLGTDHLGRCVL 60 + K A G I+ L G+FAP++ P DP ++ +E L + +YPLGTDHLGRCVL Sbjct: 14 LLKDRLALIGLGIIMLVFFTGVFAPFITPHDPDEVKLEQILLPVTADYPLGTDHLGRCVL 73 Query: 61 SRLIYGTRTSLYYSLMVLAAVFSISIPVGLLAGYVGGIIDHLIMRVIDILLAFPSLILSL 120 SRLI+GTR S+ SL+VL + I IP+G L+GY GG ID+ IMR+ID+LLAFP LIL+L Sbjct: 74 SRLIFGTRVSVSTSLLVLITIMLIGIPIGTLSGYYGGRIDNFIMRIIDVLLAFPGLILTL 133 Query: 121 AVTAVLGPSMKNLLISFAAVWWAGYARVIRSMVLQIKESDYIMAAKAGGSTHLQIILRHI 180 + +LGP++ N++I+ +AVWW GYARV+R MVL IKE +Y++ A+A G++++ I++RHI Sbjct: 134 VIAGMLGPNLINVMIALSAVWWVGYARVVRGMVLSIKEKEYVLGARAAGTSNINILVRHI 193 Query: 181 LLNATRPILVLASIEIGTLMLSIAGLSFLGLGAQPPTPEWGVMLNDSRPYIQTEPRLLLY 240 + N P++V+A++++G L+L+I+ LSFLGLGAQPPTPEWG MLND + Y+Q P L+L+ Sbjct: 194 IPNVLSPVIVMATLDMGKLILAISSLSFLGLGAQPPTPEWGAMLNDGKNYMQVAPNLMLF 253 Query: 241 PGLAIMLSVLGFNLLGEGLRK 261 PG+AIM+ VL FNLLG+GLR Sbjct: 254 PGIAIMVLVLAFNLLGDGLRD 274 >Q46F90.1 Q46F90_METBF Nickel ABC transporter, permease protein Length = 290 Score = 300 bits (769), Expect = 7e-80, Method: Compositional matrix adjust. Identities = 138/237 (58%), Positives = 190/237 (80%) Query: 29 PSDPLQIHMEHKLSMPSWEYPLGTDHLGRCVLSRLIYGTRTSLYYSLMVLAAVFSISIPV 88 P+DPL+++M +L+ PS Y LGTD+LGRC+ SRLI+G R SL YSL VL + +ISIP+ Sbjct: 54 PNDPLKVNMSMRLTDPSLLYWLGTDNLGRCIASRLIWGARMSLIYSLCVLGLMMAISIPI 113 Query: 89 GLLAGYVGGIIDHLIMRVIDILLAFPSLILSLAVTAVLGPSMKNLLISFAAVWWAGYARV 148 GLL+GY GG ID IMRVIDI L+ P+ +++LA+ +L PS +N++I+ A+VWWA YAR+ Sbjct: 114 GLLSGYAGGHIDTFIMRVIDIFLSLPTFLMALAIAGMLEPSARNMIIAMASVWWAPYARI 173 Query: 149 IRSMVLQIKESDYIMAAKAGGSTHLQIILRHILLNATRPILVLASIEIGTLMLSIAGLSF 208 IR M +QIK+ D+++AAKA TH QII RHIL N I+V+A++EIG+++L+IA SF Sbjct: 174 IRGMAMQIKQQDFMLAAKAAACTHTQIIFRHILRNIAPTIIVMATLEIGSIILAIASFSF 233 Query: 209 LGLGAQPPTPEWGVMLNDSRPYIQTEPRLLLYPGLAIMLSVLGFNLLGEGLRKPGQK 265 +GLGAQPPTPEWGVML+DS+ ++QT+P+L++YPGLAIM++V+ FNLLGEGL+ QK Sbjct: 234 IGLGAQPPTPEWGVMLSDSKEFMQTQPQLMIYPGLAIMITVMAFNLLGEGLKNAIQK 290 >C0CI01.1 C0CI01_9FIRM Putative uncharacterized protein Length = 286 Score = 292 bits (748), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 137/255 (53%), Positives = 189/255 (74%) Query: 7 AKAGGMIVFLFVCIGIFAPWLAPSDPLQIHMEHKLSMPSWEYPLGTDHLGRCVLSRLIYG 66 +K G IV LFV +FAP +AP+DPL + + HKL PS Y LGTD LGRC+ SRL++G Sbjct: 27 SKIGLAIVLLFVAAAVFAPLIAPNDPLLVDVSHKLEGPSSTYWLGTDQLGRCIASRLLWG 86 Query: 67 TRTSLYYSLMVLAAVFSISIPVGLLAGYVGGIIDHLIMRVIDILLAFPSLILSLAVTAVL 126 R SL Y +VL + +P+GL++GYVGG D MRVIDI +A PS I++LA+ L Sbjct: 87 GRNSLLYGAIVLVITLLVGVPIGLISGYVGGKTDLFFMRVIDIFMAMPSFIVALAIAGTL 146 Query: 127 GPSMKNLLISFAAVWWAGYARVIRSMVLQIKESDYIMAAKAGGSTHLQIILRHILLNATR 186 G S KNL++S + V+WAGYAR+ R++ +Q+KE +Y+MA KAGG H +II RH+L N Sbjct: 147 GASGKNLVLSMSFVYWAGYARLSRALTIQVKEQNYMMALKAGGCGHTRIIFRHVLRNIAP 206 Query: 187 PILVLASIEIGTLMLSIAGLSFLGLGAQPPTPEWGVMLNDSRPYIQTEPRLLLYPGLAIM 246 I+ LA++EIGT++L+IAG SF+GLG QPPTPEWG+ML+DS+ +IQT P+L++YPG+ I+ Sbjct: 207 SIIALATMEIGTIILAIAGFSFIGLGVQPPTPEWGIMLSDSKNFIQTFPQLMIYPGIVIV 266 Query: 247 LSVLGFNLLGEGLRK 261 + V+ FNLLGEG++ Sbjct: 267 IVVMAFNLLGEGMKN 281 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.143 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,580,359,404 Number of extensions: 61259981 Number of successful extensions: 253420 Number of sequences better than 1.0e-05: 6080 Number of HSP's gapped: 248611 Number of HSP's successfully gapped: 6113 Length of query: 268 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 135 Effective length of database: 1,807,701,208 Effective search space: 244039663080 Effective search space used: 244039663080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)