BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0199.0012.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DJ17.1 B1DJ17_9BACL Phosphate binding protein 374 e-102 C3IZC6.1 C3IZC6_9BACI Phosphate binding protein 305 2e-81 B7Y765.1 B7Y765_9BACI Phosphate binding protein 305 2e-81 Q5KUB5.1 Q5KUB5_GEOKA Phosphate ABC transporter (Substrate-bindi... 298 5e-79 A1HRT9.1 A1HRT9_9FIRM Phosphate binding protein 210 1e-52 >B1DJ17.1 B1DJ17_9BACL Phosphate binding protein Length = 288 Score = 374 bits (961), Expect = e-102, Method: Compositional matrix adjust. Identities = 182/255 (71%), Positives = 210/255 (82%), Gaps = 1/255 (0%) Query: 32 LKGKITVNGSTALLPLTLQAAKEFQKLHPKVKIAASGKGSVTGPQAVKKGIADIGACDWD 91 L GKI +NGS+ALLPLTLQAA EF+KL+PKVKI+AS GS+TGPQ+V+KGIADIGA DWD Sbjct: 35 LSGKIVINGSSALLPLTLQAANEFKKLNPKVKISASAAGSITGPQSVRKGIADIGAVDWD 94 Query: 92 ASIDVPGFKAFDGQVANKVAVIPFATIVNKNVGVDNLTTEQLQGIYSGKITNWKEVGGSD 151 A+ VPGF+AF GQVANKVAV PFA +VNK+V NLTT+QLQ I+SGK+TNWKEVGG D Sbjct: 95 ATQAVPGFEAFTGQVANKVAVTPFAAVVNKSVTATNLTTKQLQDIFSGKVTNWKEVGGKD 154 Query: 152 ASIVVVTRAFGSGTRVNYQAKALAGGDIVKKEKNYKETGSSGDMKTAVATTPNAIGYIDL 211 A I+VV RAFGSGTRVN+Q KAL G D + K NYKE +SGDMKTAV +TP AIGY+D Sbjct: 155 ADIIVVNRAFGSGTRVNFQQKALNGTDFMTKGSNYKEVKTSGDMKTAVESTPGAIGYLDF 214 Query: 212 VYVSGSDIKAVKFNGVAATTDNVINGSYKIWAYGYYMTKGQPTGATKEFIDYVQSKKFQN 271 Y++ + + V NGVA T DNVING YK+W YGYYMTKGQPTG KEFI YVQS KFQN Sbjct: 215 AYIT-TKMTGVSINGVAPTVDNVINGKYKVWGYGYYMTKGQPTGVVKEFIKYVQSAKFQN 273 Query: 272 GSLKKLKFIPIAAMK 286 GSLKKLKFIP++AMK Sbjct: 274 GSLKKLKFIPLSAMK 288 >C3IZC6.1 C3IZC6_9BACI Phosphate binding protein Length = 286 Score = 305 bits (782), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 155/251 (61%), Positives = 183/251 (72%), Gaps = 1/251 (0%) Query: 32 LKGKITVNGSTALLPLTLQAAKEFQKLHPKVKIAASGKGSVTGPQAVKKGIADIGACDWD 91 + GKI + GSTALLPLT QAAKEF+K HPKVKI SG S+ GPQ+V KG+A IGACDWD Sbjct: 36 VSGKIVIAGSTALLPLTQQAAKEFKKSHPKVKIVVSGSSSIAGPQSVAKGVATIGACDWD 95 Query: 92 ASIDVPGFKAFDGQVANKVAVIPFATIVNKNVGVDNLTTEQLQGIYSGKITNWKEVGGSD 151 A+ V GF A+ G A K+A IPFA IVNKN VDNL+ +Q+Q IY GKITNWK+VGG D Sbjct: 96 ATRAVGGFPAYKGLKAYKIAKIPFAVIVNKNNSVDNLSVQQIQDIYEGKITNWKQVGGKD 155 Query: 152 ASIVVVTRAFGSGTRVNYQAKALAGGDIVKKEKNYKETGSSGDMKTAVATTPNAIGYIDL 211 IVV+ RA GSGTRVN++ K + G I NYKE SGDM AV++ PNAIG++DL Sbjct: 156 EEIVVINRAKGSGTRVNFEDKIMDGHAIKTSGDNYKEVQKSGDMVNAVSSNPNAIGFVDL 215 Query: 212 VYVSGSDIKAVKFNGVAATTDNVINGSYKIWAYGYYMTKGQPTGATKEFIDYVQSKKFQN 271 YV G IKAV NGV ATT N +G Y + YGY +TKG+ GA K FI+Y+ SKKFQN Sbjct: 216 AYVKGG-IKAVSINGVKATTSNAASGKYVFYGYGYLLTKGEAKGANKAFINYILSKKFQN 274 Query: 272 GSLKKLKFIPI 282 GSLKKLKFIP+ Sbjct: 275 GSLKKLKFIPV 285 >B7Y765.1 B7Y765_9BACI Phosphate binding protein Length = 286 Score = 305 bits (782), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 155/251 (61%), Positives = 183/251 (72%), Gaps = 1/251 (0%) Query: 32 LKGKITVNGSTALLPLTLQAAKEFQKLHPKVKIAASGKGSVTGPQAVKKGIADIGACDWD 91 + GKI + GSTALLPLT QAAKEF+K HPKVKI SG S+ GPQ+V KG+A IGACDWD Sbjct: 36 VSGKIVIAGSTALLPLTQQAAKEFKKSHPKVKIVVSGSSSIAGPQSVAKGVATIGACDWD 95 Query: 92 ASIDVPGFKAFDGQVANKVAVIPFATIVNKNVGVDNLTTEQLQGIYSGKITNWKEVGGSD 151 A+ V GF A+ G A K+A IPFA IVNKN VDNL+ +Q+Q IY GKITNWK+VGG D Sbjct: 96 ATRAVGGFPAYKGLKAYKIAKIPFAVIVNKNNSVDNLSVQQIQDIYEGKITNWKQVGGKD 155 Query: 152 ASIVVVTRAFGSGTRVNYQAKALAGGDIVKKEKNYKETGSSGDMKTAVATTPNAIGYIDL 211 IVV+ RA GSGTRVN++ K + G I NYKE SGDM AV++ PNAIG++DL Sbjct: 156 EEIVVINRAKGSGTRVNFEDKIMDGHAIKTSGDNYKEVQKSGDMVNAVSSNPNAIGFVDL 215 Query: 212 VYVSGSDIKAVKFNGVAATTDNVINGSYKIWAYGYYMTKGQPTGATKEFIDYVQSKKFQN 271 YV G IKAV NGV ATT N +G Y + YGY +TKG+ GA K FI+Y+ SKKFQN Sbjct: 216 AYVKGG-IKAVSINGVKATTSNAASGKYVFYGYGYLLTKGEAKGANKAFINYILSKKFQN 274 Query: 272 GSLKKLKFIPI 282 GSLKKLKFIP+ Sbjct: 275 GSLKKLKFIPV 285 >Q5KUB5.1 Q5KUB5_GEOKA Phosphate ABC transporter (Substrate-binding protein) Length = 286 Score = 298 bits (762), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 154/252 (61%), Positives = 180/252 (71%), Gaps = 1/252 (0%) Query: 31 SLKGKITVNGSTALLPLTLQAAKEFQKLHPKVKIAASGKGSVTGPQAVKKGIADIGACDW 90 + GKI + GSTALLPLT QAAKEF+K HPKVKI SG S+ GPQ+V KG+A IGACDW Sbjct: 35 EVSGKIVIAGSTALLPLTQQAAKEFKKSHPKVKIVVSGSSSIAGPQSVTKGVATIGACDW 94 Query: 91 DASIDVPGFKAFDGQVANKVAVIPFATIVNKNVGVDNLTTEQLQGIYSGKITNWKEVGGS 150 DA+ V GF A+ G A K+A IPFA IVNK V NL+ EQ+Q IY GKI NWK++GG Sbjct: 95 DATKAVGGFPAYKGLKAYKIAKIPFAVIVNKGNPVSNLSVEQIQDIYEGKIKNWKQLGGK 154 Query: 151 DASIVVVTRAFGSGTRVNYQAKALAGGDIVKKEKNYKETGSSGDMKTAVATTPNAIGYID 210 D IVV+ RA GSGTRVN++ K + G I NYKE SGDM AVA+ PNAIG++D Sbjct: 155 DEEIVVINRAKGSGTRVNFEDKIMDGHMIKTTGDNYKEVQKSGDMVNAVASNPNAIGFVD 214 Query: 211 LVYVSGSDIKAVKFNGVAATTDNVINGSYKIWAYGYYMTKGQPTGATKEFIDYVQSKKFQ 270 L YV G IKAV NGV ATT N +G Y + YGY +TKG+ GA K FIDY+ SKKFQ Sbjct: 215 LAYVKGG-IKAVSINGVKATTANAKSGKYIFYGYGYLLTKGEAKGADKAFIDYILSKKFQ 273 Query: 271 NGSLKKLKFIPI 282 NGSLKKLKFIP+ Sbjct: 274 NGSLKKLKFIPV 285 >A1HRT9.1 A1HRT9_9FIRM Phosphate binding protein Length = 285 Score = 210 bits (534), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 119/256 (46%), Positives = 156/256 (60%), Gaps = 8/256 (3%) Query: 31 SLKGKITVNGSTALLPLTLQAAKEFQKLHPKVKIAASGKGSVTGPQAVKKGIADIGACDW 90 L+G +T +GSTALLPL A +EFQK HPKV + +G GS TG V G +IG D Sbjct: 36 ELQGTVTASGSTALLPLLKPAQEEFQKKHPKVTVNIAGGGSFTGMNQVAAGSVNIGNSD- 94 Query: 91 DASIDVPGFKAFDGQVANKVAVIPFATIVNKNVGVDNLTTEQLQGIYSGKITNWKEVGGS 150 +++P G V +KVAV PF I N +V VDNLT +Q I++GKITNWKEVGG Sbjct: 95 ---VELPPELKDKGLVDHKVAVAPFVFIANPDVTVDNLTQQQYIDIFTGKITNWKEVGGK 151 Query: 151 DASIVVVTRAFGSGTRVNYQAKALAGGDIVKKEKNYKETGSSGDMKTAVATTPNAIGYID 210 D I ++ RA SG+R L G N S+G ++ AVA+TP AIGY+D Sbjct: 152 DQKITIIHRAKSSGSRATIAQVVLKGAAFTD---NAVIQDSNGAVRAAVASTPGAIGYVD 208 Query: 211 LVYVSGSDIKAVKFNGVAATTDNVINGSYKIWAYGYYMTKGQPTGATKEFIDYVQSKKFQ 270 Y + +K + +NGV T +NVING+Y ++AY + TKG+PTGA K FIDYV SK+FQ Sbjct: 209 APYADNT-VKVLAYNGVKYTPENVINGTYPVFAYEHMYTKGEPTGAVKAFIDYVMSKEFQ 267 Query: 271 NGSLKKLKFIPIAAMK 286 ++K F+PI MK Sbjct: 268 ETYVEKNGFLPITKMK 283 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,537,187,601 Number of extensions: 64268336 Number of successful extensions: 122735 Number of sequences better than 1.0e-05: 1184 Number of HSP's gapped: 120892 Number of HSP's successfully gapped: 1213 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)