BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0024.p (150 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7Z2X9.1 A7Z2X9_BACA2 PksA 175 1e-42 O34381.1 PKSA_BACSU HTH-type transcriptional regulator pksA 163 4e-39 C0Z5C6.1 C0Z5C6_BREBN Transcriptional regulator 157 3e-37 C1PCM0.1 C1PCM0_BACCO Putative transcriptional regulator, TetR f... 150 4e-35 Q2BAY2.1 Q2BAY2_9BACI Transcriptional regulator 149 1e-34 >A7Z2X9.1 A7Z2X9_BACA2 PksA Length = 194 Score = 175 bits (443), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 80/147 (54%), Positives = 112/147 (76%) Query: 1 LRHYFSTQHELLAFAMNLVKDRAQARIDTVLQLDLPPKEQVTRVLMELIPLDDSTMAEME 60 LRHYF+TQ ELL +AM LVK++A ARI+ V DLPPKE+ R+++E+IP D+ TMAEME Sbjct: 46 LRHYFTTQDELLGYAMTLVKEKAAARINEVAMKDLPPKEKAVRLMLEIIPTDEETMAEME 105 Query: 61 VWFAFVFHLKYTDEEYDGLNDNIYLGIYKMVEFLDEQGILKEDLDRDVEAERLYALVDGL 120 VWFAF + ++ EE+ D I I ++++LDE +LK +LD+++EAE++YA+ DGL Sbjct: 106 VWFAFTAYARHKGEEFQPQQDGILAYIRGLIDWLDENKLLKPNLDKEIEAEKMYAIADGL 165 Query: 121 ALHAMLEPERLDKQRIIRVLTGHLDSI 147 ALHAMLEPERL K++I RVL H++S+ Sbjct: 166 ALHAMLEPERLSKEKISRVLVQHIESL 192 >O34381.1 PKSA_BACSU HTH-type transcriptional regulator pksA Length = 205 Score = 163 bits (413), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 76/148 (51%), Positives = 111/148 (75%) Query: 1 LRHYFSTQHELLAFAMNLVKDRAQARIDTVLQLDLPPKEQVTRVLMELIPLDDSTMAEME 60 LRHYFSTQ ELLAFAM LV+++ RI + DL PKE+V ++L+E++P ++ T+ EME Sbjct: 46 LRHYFSTQDELLAFAMKLVQEKVTDRIKDIAVRDLLPKEKVLQILLEMVPTNEETIREME 105 Query: 61 VWFAFVFHLKYTDEEYDGLNDNIYLGIYKMVEFLDEQGILKEDLDRDVEAERLYALVDGL 120 VWFAF + ++ + +D +D I+ G+ ++ +LDE +LK++ D+D+EAERLYALVDGL Sbjct: 106 VWFAFTAYARHKKDMFDASHDGIFSGMRNLIAYLDESDLLKQNADKDIEAERLYALVDGL 165 Query: 121 ALHAMLEPERLDKQRIIRVLTGHLDSIS 148 ALHAML+P R++K RI RV+ H++SI Sbjct: 166 ALHAMLDPVRVNKDRIKRVIMQHVESIC 193 >C0Z5C6.1 C0Z5C6_BREBN Transcriptional regulator Length = 195 Score = 157 bits (397), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 73/148 (49%), Positives = 107/148 (72%), Gaps = 1/148 (0%) Query: 1 LRHYFSTQHELLAFAMNLVKDRAQARI-DTVLQLDLPPKEQVTRVLMELIPLDDSTMAEM 59 LRHYF+TQ ELL +AM LVK+RA ARI D + PKE+VT++ +EL+P + M EM Sbjct: 46 LRHYFATQDELLMYAMQLVKERATARIADIAANEEWAPKERVTKIFLELLPTNQEKMVEM 105 Query: 60 EVWFAFVFHLKYTDEEYDGLNDNIYLGIYKMVEFLDEQGILKEDLDRDVEAERLYALVDG 119 EVWFAF + ++ E +D +D IY G+ K+++ D+ +L++ LD+++E E+LYA++DG Sbjct: 106 EVWFAFTVYFRHKREGFDAQHDGIYAGVRKLLDSADQMNLLRKGLDKEIETEKLYAVIDG 165 Query: 120 LALHAMLEPERLDKQRIIRVLTGHLDSI 147 LALHA LEP+R+D +RI +VL HL S+ Sbjct: 166 LALHAYLEPQRVDGERITKVLEHHLASL 193 >C1PCM0.1 C1PCM0_BACCO Putative transcriptional regulator, TetR family Length = 168 Score = 150 bits (379), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 71/148 (47%), Positives = 104/148 (70%), Gaps = 1/148 (0%) Query: 1 LRHYFSTQHELLAFAMNLVKDRAQARIDTVLQLDLPPKEQVTRVLMELIPLDDSTMAEME 60 LRHYFSTQ ELL +AM+LVK+R R++ + +LPPKE + ++L+E++P +++T AEME Sbjct: 20 LRHYFSTQEELLIYAMDLVKERVAERVEKIALKNLPPKEMILQMLLEIVPTNETTRAEME 79 Query: 61 VWFAFVFHLKYTDEEYDGLNDNIYLGIYKMVEFLDEQGILKEDLDRDVEAERLYALVDGL 120 VW FV + + + ND I GI K+++ LD LK+++D+D+E ERL AL+DGL Sbjct: 80 VWLEFVLYFRNKGNR-ESQNDGILEGIQKLLDSLDRAQFLKKEIDKDLETERLCALIDGL 138 Query: 121 ALHAMLEPERLDKQRIIRVLTGHLDSIS 148 A+HA+L P RL K+RII +L H+D I Sbjct: 139 AIHAILNPTRLTKERIISILAHHIDEIC 166 >Q2BAY2.1 Q2BAY2_9BACI Transcriptional regulator Length = 193 Score = 149 bits (375), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 77/149 (51%), Positives = 100/149 (67%), Gaps = 1/149 (0%) Query: 1 LRHYFSTQHELLAFAMNLVKDRAQARIDTVLQLDLPPKEQVTRVLMELIPLDDSTMAEME 60 LRHYFSTQ ELL +AM LV + R+ + DLPP+E+V +VL+E++P D MAEME Sbjct: 46 LRHYFSTQDELLVYAMKLVNENVINRVSEIALKDLPPREKVYQVLLEIVPTTDEKMAEME 105 Query: 61 VWFAFVFHLKYTDEEYDGLNDNIYLGIYKMVEFLDEQGILKEDLDRDVEAERLYALVDGL 120 VWFAFV + + EE+ L IY GI +M++FL G LK+ D D+E E LY+ +DGL Sbjct: 106 VWFAFVAYSR-QKEEFRILKQGIYTGIVQMMDFLKSHGELKDGQDHDLETEMLYSFIDGL 164 Query: 121 ALHAMLEPERLDKQRIIRVLTGHLDSISK 149 ALH+MLEPERL RI + L +LD I K Sbjct: 165 ALHSMLEPERLKPDRIEKTLRRYLDRILK 193 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.140 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 851,270,477 Number of extensions: 33508748 Number of successful extensions: 81808 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 81743 Number of HSP's successfully gapped: 59 Length of query: 150 Length of database: 3,060,696,203 Length adjustment: 112 Effective length of query: 38 Effective length of database: 2,005,542,523 Effective search space: 76210615874 Effective search space used: 76210615874 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)