BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0016.p (247 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8MBT7.1 A8MBT7_CALMQ Xylose isomerase domain protein TIM barrel 63 3e-08 C2CHU0.1 C2CHU0_9FIRM Myo-inosose-2 dehydratase EC=4.2.1.44 63 3e-08 Q01WU7.1 Q01WU7_SOLUE Xylose isomerase domain protein TIM barrel 57 2e-06 C5F6H7.1 C5F6H7_LACPA Inosose dehydratase 55 5e-06 >A8MBT7.1 A8MBT7_CALMQ Xylose isomerase domain protein TIM barrel Length = 266 Score = 62.8 bits (151), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 63/237 (26%), Positives = 105/237 (44%), Gaps = 35/237 (14%) Query: 20 LPLPEVLRLVRGYGIQAVELDLDE-------VQPSPEAMKNLLEQAGISVASM--YAFFD 70 L L EVL VR +G +EL + E + P P+ L++ +GI ++++ YA F Sbjct: 13 LTLSEVLERVRRFGFDGLELRVAEDGQHLKPIYPVPKQDYELIKSSGIKLSNLAGYASFA 72 Query: 71 FGN----APDVRPGLTFIDTAAFLGAGKVLVIPGFIDEPADQELRERSLRHMAAALREMS 126 N A + T I A L A V V G + + E++ + +L + Sbjct: 73 MPNEQERARNEETLRTLILIARELEATGVRVYGGRVTDDV-----EKAAERIITSLNRVV 127 Query: 127 EYAGRKGLVVTLEDFDDSRAPFSTSGELLWFLNEVPK-LGITFDTGNFIYRGEDALEAFE 185 ++A G+V+ +E DD + + L LN + + +GI D N + GE+ E Sbjct: 128 KFAEDNGVVIMMETHDD----WVKANNLRRLLNGLDEGIGILLDFANVVAAGENLDNVLE 183 Query: 186 KLKSRIVHVHCKDRSLEEHPGETPKISTNGTRLFPSPVG-SGCIPIEEILDRLEASG 241 +K R+ HVH K+ L NG + +P +G +PI+ ++D L G Sbjct: 184 LVKGRVRHVHVKNFKL-----------INGKIRYTTPDDPAGLVPIKRVVDYLRGIG 229 >C2CHU0.1 C2CHU0_9FIRM Myo-inosose-2 dehydratase EC=4.2.1.44 Length = 300 Score = 62.8 bits (151), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 56/212 (26%), Positives = 88/212 (41%), Gaps = 21/212 (9%) Query: 48 PEAMKNLLEQAGISVASMYAFFDFGNAPDVRPGLTFIDTAAFLGA--GKVLV-------I 98 PE +K+ LE + VAS + P FI+ FL A KV+V I Sbjct: 51 PEVLKSYLEPRNLEVASAWFSAFLTTKPYEETKKAFIEHRDFLHAMGAKVIVVAEQGHSI 110 Query: 99 PGFIDEPADQE---LRERSLRHMAAALREMSEYAGRKGLVVTLEDFDDSRAPFSTSGELL 155 G +D P + + +A L ++ E A KG+ + + + E L Sbjct: 111 QGQMDTPVFSKKPTFTDEEWDRLACGLNKLGELALEKGMKIVYHHHMGTGVQTTAEVEKL 170 Query: 156 WFLNEVPKLGITFDTGNFIYRGEDALEAFEKLKSRIVHVHCKD------RSLEEHPGETP 209 + + K+ + +DTG+ ++ GED + ++K RI H+H KD + +E Sbjct: 171 MEMTDPEKVFLLYDTGHLVFSGEDPIAFYKKFSKRIKHIHFKDIRAEKQKQSKEEEWSFL 230 Query: 210 KISTNGTRLFPSPVGSGCIPIEEILDRLEASG 241 K GT PS GCI E I+D + G Sbjct: 231 KSVFQGTFTVPS---DGCIDFETIIDMILEDG 259 >Q01WU7.1 Q01WU7_SOLUE Xylose isomerase domain protein TIM barrel Length = 266 Score = 56.6 bits (135), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 58/212 (27%), Positives = 85/212 (40%), Gaps = 39/212 (18%) Query: 52 KNLLEQAGISVASMYAFF---DFGNAPDVRPGLTFI----------------DTAAFLGA 92 K L AGI+ ++ A + D+ + P V+ + FI D AA LG Sbjct: 40 KQALSSAGITPVTVVAAYEGEDYADIPTVQHTVGFIPPATRAAREARTLAVSDFAAALGV 99 Query: 93 GKVLVIPGFIDEPADQELRERSLRHMAAALREMSEYAGRKGLVVTLEDFDDSRAPFSTSG 152 + GF+ E A + + +R + ++A R G LE + +G Sbjct: 100 RSIACHIGFVPEDA----HDPGYTGVRDTVRHVCDHAARHGQTFALETGQER------AG 149 Query: 153 ELLWFLNEV--PKLGITFDTGNFIYRGED-ALEAFEKLKSRIVHVHCKDRSLEEHPGETP 209 LL F+++V P L I FD N I G D L A + L +V VHCKD G+ P Sbjct: 150 VLLDFIHDVDRPNLRINFDPANLILYGTDEPLAALKTLAPLVVSVHCKD-------GDPP 202 Query: 210 KISTNGTRLFPSPVGSGCIPIEEILDRLEASG 241 G P+G G + I + L G Sbjct: 203 PAGIPGALGSERPLGHGSVGIPRFIQTLREVG 234 >C5F6H7.1 C5F6H7_LACPA Inosose dehydratase Length = 298 Score = 55.5 bits (132), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 49/211 (23%), Positives = 88/211 (41%), Gaps = 17/211 (8%) Query: 47 SPEAMKNLLEQAGISVASMYAFFDFGNAPDVRPGLTFIDTAAFLGA--GKVLVIP----- 99 P+A+K+ L+ + +AS + P FI FL A KV+V+ Sbjct: 50 DPKALKHFLDLRHLKIASAWFSAFLTTKPYEETEAAFIKHRDFLHAMGAKVIVVAEQGHS 109 Query: 100 --GFIDEPADQE---LRERSLRHMAAALREMSEYAGRKGLVVTLEDFDDSRAPFSTSGEL 154 G +D+ + + +A L + + A G+ + + + + Sbjct: 110 VQGLLDKSVFDDKPHFTDDEWERLATGLERLGDRAREVGMQIVYHHHMGTGVQTTAEIDR 169 Query: 155 LWFLNEVPKLGITFDTGNFIYRGEDALEAFEKLKSRIVHVHCKD----RSLEEHPGETPK 210 L + + K+ + FDTG+ + GED L F + + RI H+H KD ++ EEH Sbjct: 170 LMTMTDPDKVSLLFDTGHLVLSGEDPLTIFNRYQDRIKHIHFKDVRQQQADEEHKDHLSF 229 Query: 211 ISTNGTRLFPSPVGSGCIPIEEILDRLEASG 241 ++ +F P G G I + I + ++ SG Sbjct: 230 LAGVKNGMFTVP-GDGMIDFKPIWEAIQESG 259 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,478,279,501 Number of extensions: 64373059 Number of successful extensions: 174263 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 174301 Number of HSP's successfully gapped: 22 Length of query: 247 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 115 Effective length of database: 1,817,122,223 Effective search space: 208969055645 Effective search space used: 208969055645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)