BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0010.p (430 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4DLE9.1 C4DLE9_9ACTO Amidase, Asp-tRNAAsn/Glu-tRNAGln amidotran... 256 2e-66 A7U0U6.1 A7U0U6_9EURY Possible amidase 249 5e-64 B0VFY9.1 B0VFY9_9BACT Putative amidase EC=3.5.1.4 239 4e-61 C1T2W1.1 C1T2W1_DESBA Amidase, Asp-tRNAAsn/Glu-tRNAGln amidotran... 238 7e-61 Q11LH8.1 Q11LH8_MESSB Amidase 174 2e-41 >C4DLE9.1 C4DLE9_9ACTO Amidase, Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit Length = 424 Score = 256 bits (655), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 152/402 (37%), Positives = 218/402 (54%), Gaps = 6/402 (1%) Query: 30 ERYERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGTEAKPAFYGVPVGIKDLLHVEGLL 89 +R E + I AF E + RL A A+ + + +PA YGVPVG+KD++HV+GL Sbjct: 26 DRIEATDAEIFAFESEPDRRGRLLAEAQATEERWGEPADRPALYGVPVGVKDVIHVDGLE 85 Query: 90 TRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAYNGPIATRNPHDLSHTPXXX 149 TRAGS +PA L+G + +L+ RL GALIAGK EFA + P TRNPH+ +HTP Sbjct: 86 TRAGSKVPAEALAGAQATLVDRLVGAGALIAGKNTCAEFAISAPGPTRNPHNPTHTPGGS 145 Query: 150 XXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSYGRVPLDGVILLAPSFDTIG 209 TQT+ S I PA++CG+VGFKPS+GR+P+DGVI APS DTIG Sbjct: 146 SSGSAAAVAAGQVRLSIGTQTVSSTIRPAAYCGIVGFKPSHGRIPVDGVIANAPSLDTIG 205 Query: 210 FFTQNVSDMQAAAALLIPDWNHGSRQAGRKPVLGIPRGIYMELMSGGVKAAFTAQIEGLQ 269 T +V+ A+ +L +W+ R PVLGIP Y+E S A F + L+ Sbjct: 206 ILTTDVAGAAIASEVLCDEWS--PRAVDELPVLGIPADEYLECASKPTLARFAEHVAKLK 263 Query: 270 QKGYTVKQVNMPWEDEFIYGDAMLRFLEG-EMAREHAAWFERFADIYGAPVREAILRGRE 328 GY V V +F A+L ++ E A HAAWF+ + +Y +++L GR+ Sbjct: 264 AAGYRV--VPGRAVTDFPRIRAVLFTIQRYESAIVHAAWFDSYRALYQPKSAQSVLEGRD 321 Query: 329 ITDTELEMYRRRQARLCRELETARAAQGVDLWVSPAQGGTAPKIGEGTGWPGMTAIWTFA 388 I + + ++ + + + ++ GVD+W+ P+ TAP TG M+ W+ Sbjct: 322 IDEDKHQLALKLRTAIHDQIRDMMERSGVDIWIGPSATDTAPVGLASTGNSIMSIPWSLT 381 Query: 389 GCPAVSLPSASIDNLPLGFQCIGSYGQDEALAAWAQQISEDL 430 G P++S+P+ + D LPLGF C G DE L AW I+ L Sbjct: 382 GMPSLSVPAPAPD-LPLGFHCAARTGDDERLLAWGGDIAATL 422 >A7U0U6.1 A7U0U6_9EURY Possible amidase Length = 432 Score = 249 bits (635), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 160/390 (41%), Positives = 211/390 (54%), Gaps = 5/390 (1%) Query: 30 ERYERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGTEAKPAFYGVPVGIKDLLHVEGLL 89 +R + +EP + AF E ER+ +AA+ L + +P YGVPVG+KD+ V+GL Sbjct: 34 DRLDAVEPEVEAFVSEGGRRERMLSAAERLPEAP--PPERPPLYGVPVGVKDVFRVDGLP 91 Query: 90 TRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAYNGPIATRNPHDLSHTPXXX 149 T AGS LPAA L+G E ++I RLR+ GA++ GKTVT EFAY P TRNPHD +HTP Sbjct: 92 TGAGSDLPAAALAGPEAAVIERLRAAGAVVLGKTVTTEFAYFHPGPTRNPHDTAHTPGGS 151 Query: 150 XXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSYGRVPLDGVILLAPSFDTIG 209 TQT S I PA+FCGVVG KPSYGR+ DG++ LAPS D +G Sbjct: 152 SSGSAAAVAAGEVPVALGTQTAGSTIRPAAFCGVVGMKPSYGRILTDGLLPLAPSLDHVG 211 Query: 210 FFTQNVSDMQAAAALLIPDWNHGSRQAGRKPVLGIPRGIYMELMSGGVKAAFTAQIEGLQ 269 FT V DM+ AA++ +W + A PVL +P G Y+E F ++ L+ Sbjct: 212 LFTAAVDDMRLAASVCCDEWR--AIDAPNDPVLAVPDGPYLEQAEQAGLTGFERAVDALE 269 Query: 270 QKGYTVKQVNMPWEDEFIYGDAMLRFLEGEMAREHAAWFERFADIYGAPVREAILRGREI 329 GY V++V+ ED D L GE A H W+ AD Y + + RGR + Sbjct: 270 AAGYGVRRVDA-LEDVETVNDRHTTLLAGEAALVHHDWYTAHADAYHGTRVDLVERGRGV 328 Query: 330 TDTELEMYRRRQARLCRELETARAAQGVDLWVSPAQGGTAPKIGEGTGWPGMTAIWTFAG 389 + L R + L L A G D W++PA G AP+ E TG M WT AG Sbjct: 329 STEALATARASRVALRERLTERLTAAGADCWLAPAAPGPAPEGLESTGDVAMNLPWTHAG 388 Query: 390 CPAVSLPSASIDNLPLGFQCIGSYGQDEAL 419 P VSLP+ ++D LPLG Q +G +G DE L Sbjct: 389 LPVVSLPAGAVDGLPLGVQIVGRFGADEYL 418 >B0VFY9.1 B0VFY9_9BACT Putative amidase EC=3.5.1.4 Length = 429 Score = 239 bits (610), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 141/395 (35%), Positives = 211/395 (53%), Gaps = 5/395 (1%) Query: 33 ERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGTEAKPAFYGVPVGIKDLLHVEGLLTRA 92 E+ EP + A E N ERL A+ LL + E +P F+GV +G+KDL +V+GL TRA Sbjct: 36 EKKEPVLCALEPEENRQERLLKQAEELLSRFPQPELRPPFFGVLIGVKDLFNVDGLSTRA 95 Query: 93 GSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAYNGPIATRNPHDLSHTPXXXXXX 152 GS LP V SG+E ++ RL++LGALI GKT EFAY P T+NP++ +HTP Sbjct: 96 GSKLPPEVFSGKEAEIVTRLKNLGALILGKTTCTEFAYFQPAKTKNPYNPNHTPGGSSSG 155 Query: 153 XXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSYGRVPLDGVILLAPSFDTIGFFT 212 TQT+ S+I PAS+CGV G KPSYG L GV + + D +GF T Sbjct: 156 SAAAVSAGYCLLALGTQTIGSIIRPASYCGVTGLKPSYGLFSLQGVFPFSQTVDHLGFIT 215 Query: 213 QNVSDMQAAAALLIPDWNHGSRQAGRKPVLGIPRGIYMELMSGGVKAAFTAQIEGLQQKG 272 + V+D+ LL S +PVLG G Y++ V+ + + ++ LQ+KG Sbjct: 216 KQVADLAFLLPLLHLSV---SATITDRPVLGAVNGSYLQQTDSSVQENYCSALDKLQKKG 272 Query: 273 YTVKQVNMPWEDEFIYGDAMLRFLEGEMAREHAAWFERFADIYGAPVREAILRGREITDT 332 Y + + P+ D + + E + H + +ER+ ++Y +E RG +I+ Sbjct: 273 YKIVTCD-PFGDINSINASHNALIAAEFSLNHKSLYERYQELYSDSSKELYARGLQISSA 331 Query: 333 ELEMYRRRQARLCRELETARAAQGVDLWVSPAQGGTAPKIGEGTGWPGMTAIWTFAGCPA 392 ELE+ R++ L ++ Q +DLW++P+ TAP + TG P M+ WT G PA Sbjct: 332 ELEILRQQTIILREKIINLMQEQKIDLWITPSTTTTAPAGLKSTGSPLMSLPWTNCGLPA 391 Query: 393 VSLPSA-SIDNLPLGFQCIGSYGQDEALAAWAQQI 426 +++P+ + LP G Q + SY +D L Q+I Sbjct: 392 LTIPNGLDANGLPFGLQFVASYNEDLPLVRMVQRI 426 >C1T2W1.1 C1T2W1_DESBA Amidase, Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit Length = 436 Score = 238 bits (608), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 150/409 (36%), Positives = 205/409 (50%), Gaps = 11/409 (2%) Query: 14 PDRMQNKLHPSWDLFRERYERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGTEAKPAFY 73 PD +Q+ +H L R L HI A ++ A +R + L Y +P + Sbjct: 21 PDALQSLVHEHMRLARSVDPTL--HILAARIDEQAAQR---RIEDLFTQYPDPARRPPLF 75 Query: 74 GVPVGIKDLLHVEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAYNGP 133 GVPVG+KD+ ++G R GS LP +G+E +R LR GA+I +T T EFAY P Sbjct: 76 GVPVGVKDIFRIDGASIRCGSLLPPVFFAGEEARCVRMLRDAGAIIFAQTATTEFAYFEP 135 Query: 134 IATRNPHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSYGRV 193 ATRNPH+ +HTP TQT+ S++ PA+FCGVVG KPS G++ Sbjct: 136 AATRNPHNPAHTPGGSSSGSAAGVAAGFFPLALGTQTVGSIVRPAAFCGVVGMKPSLGKI 195 Query: 194 PLDGVILLAPSFDTIGFFTQNVSDMQAAAALLIPDWNHGSRQAGRKPVLGIPRGIYMELM 253 P GV+ +P+ D +GFF + V+D+ A P W G + LG+P G Y+ + Sbjct: 196 PTAGVVHFSPTVDHVGFFCRQVADIPPVMAAFDPHWTTGGSLGQVR--LGLPVGKYLNQL 253 Query: 254 SGGVKAAFTAQIEGLQQKGYTVKQVNMPWEDEFIYGDAMLR-FLEGEMAREHAAWFERFA 312 A F + L+ +G+ V +V P D+ A R + E A E AWF +A Sbjct: 254 PAETMAWFEGVVATLEAEGFEVLRV--PCLDDIEEIAARHRDLIAAEFALEQEAWFTDYA 311 Query: 313 DIYGAPVREAILRGREITDTELEMYRRRQARLCRELETARAAQGVDLWVSPAQGGTAPKI 372 +Y R+ I +GR I + L R A L REL G+DLW+ P+ G AP Sbjct: 312 HLYRPATRDIIQKGRTIPEARLAEIRESCAALRRELCAVMDGHGLDLWICPSTLGEAPAG 371 Query: 373 GEGTGWPGMTAIWTFAGCPAVSLPS-ASIDNLPLGFQCIGSYGQDEALA 420 GTG P M WT AG P +SLP LPLG QCIG +G+D LA Sbjct: 372 LGGTGSPAMNLPWTHAGLPVISLPCGRGGRGLPLGLQCIGRFGEDARLA 420 >Q11LH8.1 Q11LH8_MESSB Amidase Length = 450 Score = 174 bits (441), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 133/363 (36%), Positives = 182/363 (50%), Gaps = 21/363 (5%) Query: 71 AFYGVPVGIKDLLHVEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAY 130 A +GVPVGIKD++ V G+ T GS + A + ++ +L+RRLR+ GA+I GKTVT EFAY Sbjct: 78 ALHGVPVGIKDIIDVRGMPTGNGSPIDAGRMPFEDSTLVRRLRAAGAVILGKTVTTEFAY 137 Query: 131 NGPIATRNPHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSY 190 P TRNPHD + TP +QT SVI PASFCGVVG KPS+ Sbjct: 138 YQPGKTRNPHDPARTPGGSSSGSAAAVAAGMVPLAIGSQTNGSVIRPASFCGVVGLKPSF 197 Query: 191 GRVPLDGVILLAPSFDTIGFFTQNVSDMQAAAALLIPDW-NHGSRQA------------G 237 G +P GV+ LAPS D +G F +++ D A L+ D + +R++ Sbjct: 198 GAIPRTGVLRLAPSLDHMGVFARSLEDAAFAEILMGADGRDPDARESPGPLSATALSDPP 257 Query: 238 RKPVLGIPRGIYMELMSGGVKAAFTAQIEGLQQKGYTVKQVNMPWEDEFIYGDAMLRFLE 297 P L R E KAAFT +E L G + QV++ +G + Sbjct: 258 VTPALAFVRTPAWERAEEPTKAAFTELVEAL---GSGIDQVDLSEHFAGAFGWHQT-VMS 313 Query: 298 GEMAREHAAWFERFADIYGAPVREAILRGREITDTELEMYRRRQARLCRELETARAAQGV 357 EMAR E D+ +R + GR I + + R A + RE T + Sbjct: 314 AEMARNLGHHVEHMPDLCSEKLRAIVAEGRAIAAPDY-LAARDMAPVLRETLTP-IFERF 371 Query: 358 DLWVSPAQGGTAPKIGEGTGWPGMTAIWTFAGCPAVSLPSASIDN-LPLGFQCIGSYGQD 416 D ++PA G AP +GE TG P +WTF G P++SLP + N LP+G Q +G+ GQD Sbjct: 372 DAIITPAAPGEAP-VGEATGDPVFCTLWTFCGLPSLSLPLLNGPNGLPVGVQLVGAPGQD 430 Query: 417 EAL 419 L Sbjct: 431 ARL 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,525,656,265 Number of extensions: 107282032 Number of successful extensions: 256194 Number of sequences better than 1.0e-05: 3035 Number of HSP's gapped: 252390 Number of HSP's successfully gapped: 3040 Length of query: 430 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 291 Effective length of database: 1,751,175,118 Effective search space: 509591959338 Effective search space used: 509591959338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)