BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0009.p (418 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8EMJ3.1 Q8EMJ3_OCEIH Intracellular alkaline serine proteinase 332 6e-89 Q8ENV1.1 Q8ENV1_OCEIH Intracellular alkaline serine proteinase 316 3e-84 A8MKI5.1 A8MKI5_ALKOO Peptidase S8 and S53 subtilisin kexin sedo... 310 2e-82 A6TXA8.1 A6TXA8_ALKMQ Peptidase S8 and S53, subtilisin, kexin, s... 308 5e-82 C0GDG5.1 C0GDG5_9FIRM Peptidase S8 and S53 subtilisin kexin sedo... 307 1e-81 >Q8EMJ3.1 Q8EMJ3_OCEIH Intracellular alkaline serine proteinase Length = 435 Score = 332 bits (850), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 178/395 (45%), Positives = 239/395 (60%), Gaps = 6/395 (1%) Query: 2 REKLWVKQYASKMN----RPLRNEIRKTFHSGKSSAALPVIVQFKHKITPSRMLALHKHL 57 R+K+W + K++ LRN RK + + +P+IV K + L K Sbjct: 4 RKKVWFEDAQRKLDPGLVEQLRNS-RKVDQNETINNEIPLIVYMKKGCERGKKDDLLKAC 62 Query: 58 GAHSFAIKHR-IPMLNALSSRVSVNCLKRLCCWEGVHKVHLDHIKSISLDIATPSIGAAA 116 S HR + +NA+ ++ + ++++ E V ++ D I + LDIA+ GA Sbjct: 63 NIDSHNKLHRELRSINAMKGYLTPDRIQQIKDHEAVDRIFYDRIVTAFLDIASEQTGAFK 122 Query: 117 VQSKRGLTGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGHGTHITGD 176 VQ + GLTGK + IAV+DTGV H DL P NRIVAF+DF+N PYDDNGHGTH GD Sbjct: 123 VQEQLGLTGKDVTIAVIDTGVHAHADLTDPTNRIVAFQDFINGESEPYDDNGHGTHCAGD 182 Query: 177 AAGNGWSSRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCIAEKKRLKLRILSMS 236 AAGNG S GKY GPAPEA I+G+KVLD G G STII+GIEWC+ K+ +RI+S+S Sbjct: 183 AAGNGSLSNGKYIGPAPEASIIGIKVLDEQGSGRLSTIIEGIEWCVDHKEEHNIRIISLS 242 Query: 237 FGGPISTSCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGISPAAVTVGAVDDR 296 G P S RDD L A + A G+ V AAGN GP TI +P I P +TVG+ +D+ Sbjct: 243 LGAPAYESFRDDPLSLAAQSAWHQGIVVCAAAGNDGPAASTISTPAIDPFIITVGSTNDQ 302 Query: 297 RTLPQKDDRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLARNFPYLRIGKQYFV 356 TL + DD I YSSRGPT +KPD+ APG ++ISL APGS L P + I + Y Sbjct: 303 NTLERTDDAIAEYSSRGPTIDTLVKPDVYAPGTNIISLSAPGSALESQIPEMIIDENYIQ 362 Query: 357 LSGTSVSTPIVSGAAAQLLQSCPSLTPRQVKARLK 391 LSGTS++TPI +G A +L++ P+L+P +K+ LK Sbjct: 363 LSGTSMATPICAGIIALMLEANPNLSPNDIKSILK 397 >Q8ENV1.1 Q8ENV1_OCEIH Intracellular alkaline serine proteinase Length = 430 Score = 316 bits (810), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 176/388 (45%), Positives = 232/388 (59%), Gaps = 11/388 (2%) Query: 30 KSSAALPVIVQFKHKITPSRMLALH--KHLGAHSFAIKHRIPMLNALSSRVSVNCLKRLC 87 K + LPV+++FK + + LH K+ S H I +A S + L C Sbjct: 48 KKTKKLPVVIKFK---SDNFANGLHEVKNTKCKSLREFHSISCCSAKFSAEKIEQLIENC 104 Query: 88 CWEGVHKVHLDHIKSISLDIATPSIGAAAVQSKRGLTGKGINIAVLDTGVFPHPDLVRPV 147 + K++ D LD A+ SI A ++ + GLTG+G IAV+DTG+ PH DL Sbjct: 105 SL--IEKIYYDRKVFSLLDTASSSINADVLK-ESGLTGQGSTIAVIDTGIHPHEDLE--- 158 Query: 148 NRIVAFKDFVNHRKRPYDDNGHGTHITGDAAGNGWSSRGKYRGPAPEAGIVGVKVLDING 207 RI+ F DFV + PYDDNGHGTH GDAAGNG S G+Y+GPAP+A +VGVKVL+ G Sbjct: 159 GRIIGFADFVKGQTEPYDDNGHGTHCAGDAAGNGALSDGQYQGPAPDANLVGVKVLNKTG 218 Query: 208 DGYDSTIIKGIEWCIAEKKRLKLRILSMSFGGPISTSCRDDLLCQAVEKAVQAGLTVVIA 267 G ST+I+GI+WCI + + + ILS+S G + D + AVE A G+ V +A Sbjct: 219 SGSLSTVIEGIDWCIQNQSKYNINILSLSLGSDATEPAEGDPVVNAVETAWDNGMVVCVA 278 Query: 268 AGNSGPGRKTIESPGISPAAVTVGAVDDRRTLPQKDDRITFYSSRGPTPCGKIKPDLVAP 327 AGNSGPG KT+ SPGISP +TVGA DD T + DD + +SSRGPT G KP+L+ P Sbjct: 279 AGNSGPGDKTVGSPGISPKVITVGAADDNNTAERSDDSVAEFSSRGPTIDGLTKPNLLTP 338 Query: 328 GESVISLRAPGSQLARNFPYLRIGKQYFVLSGTSVSTPIVSGAAAQLLQSCPSLTPRQVK 387 G ++SLRAPGS + + R+G Y LSGTS++TPI +G AQLLQS SLTP QVK Sbjct: 339 GVDIVSLRAPGSFIDKTNKSARVGSNYISLSGTSMATPICAGIVAQLLQSDSSLTPNQVK 398 Query: 388 ARLKRNAFRLGLKPNTAGSGEINMRFLL 415 +L LG PN G+G +N L+ Sbjct: 399 EKLMEACQDLGQSPNVQGAGYLNAANLI 426 >A8MKI5.1 A8MKI5_ALKOO Peptidase S8 and S53 subtilisin kexin sedolisin Length = 426 Score = 310 bits (793), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 163/354 (46%), Positives = 231/354 (65%), Gaps = 14/354 (3%) Query: 64 IKHRIPMLNALSSRVSVNCLKRLCCWEGVHKVHLDHIKSISLDIATPSIGAAAVQSKRGL 123 +K+++P++NA+++ + +K + K++LD +DIA+ ++GA + ++RGL Sbjct: 49 VKYKLPIINAIAAYMPSVNVKSMAMERVTEKIYLDDYAYKLMDIASVTVGAD-LANERGL 107 Query: 124 TGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGHGTHITGDAAGNGWS 183 TGK ++IAV+DTGVFPH DL P NRIVAFKDFV ++ PYDD+GHGTH+ G AGNG+S Sbjct: 108 TGKNVSIAVVDTGVFPHRDLTTPNNRIVAFKDFVGEKREPYDDDGHGTHVAGIVAGNGFS 167 Query: 184 SRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCIAEKKRLKLRILSMSFGGPIST 243 S+GKY G AP+A IVGVKVL +G G S +I G++W I +++ ++++++S G Sbjct: 168 SKGKYMGIAPDANIVGVKVLGGDGGGSISDVIAGVQWVIQNREKYNIKVMTLSLGTKPKG 227 Query: 244 SCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGISPAAVTVGAVDDRRTLPQKD 303 S +D LC+AV AV +G+TVV+AAGNSGP TI SP ISP +TVGA DDR+ K+ Sbjct: 228 SYTEDPLCKAVHMAVDSGITVVVAAGNSGPNPSTITSPAISPKVITVGACDDRKASNSKN 287 Query: 304 DRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLARNFPYLRIGKQYFVLSGTSVS 363 I +SSRGPTP G KPD++APG + SL +Y LSGTS++ Sbjct: 288 ITIANFSSRGPTPDGLKKPDILAPGVGINSLANKQG-------------EYHTLSGTSMA 334 Query: 364 TPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRLGLKPNTAGSGEINMRFLLGK 417 TP+V+G AA L +S P +TP +K L NA LGLKP G+G +N+R + K Sbjct: 335 TPVVAGCAALLYESNPHITPGAIKETLLSNAHSLGLKPEEQGNGVLNIRNIANK 388 >A6TXA8.1 A6TXA8_ALKMQ Peptidase S8 and S53, subtilisin, kexin, sedolisin Length = 430 Score = 308 bits (790), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 159/352 (45%), Positives = 233/352 (66%), Gaps = 14/352 (3%) Query: 64 IKHRIPMLNALSSRVSVNCLKRLCCWEGVHKVHLDHIKSISLDIATPSIGAAAVQSKRGL 123 IK+ +P++N++++ + ++ + E ++K+ D + +D+A+ ++G+ ++ L Sbjct: 49 IKYLLPLINSVAAYLPSVGVRSVAREEFINKIIYDELAHKLMDVASLTVGSD-YANEYDL 107 Query: 124 TGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGHGTHITGDAAGNGWS 183 TGKG+ +AV+DTGV PH DLV P NRI+ FKDFV R PYDD+GHGTH+ G AGNG+S Sbjct: 108 TGKGVTVAVIDTGVHPHSDLVTPTNRIIGFKDFVGKRTSPYDDDGHGTHVAGIVAGNGFS 167 Query: 184 SRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCIAEKKRLKLRILSMSFGGPIST 243 S+GKY G AP+A I+GVKVL+ +G G S +I GI+W + + R ++++++S G + Sbjct: 168 SQGKYMGIAPDANILGVKVLNQDGGGSISDVIAGIQWAVDNRSRYNVKVMTLSLGTKANK 227 Query: 244 SCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGISPAAVTVGAVDDRRTLPQKD 303 S R+D LCQAVE AV AG+TVV AAGNSGP R TI SP SP+A++VGAVDDR +D Sbjct: 228 SYREDPLCQAVEAAVNAGITVVTAAGNSGPERSTINSPANSPSAISVGAVDDRDITSPRD 287 Query: 304 DRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLARNFPYLRIGKQYFVLSGTSVS 363 +I +SSRGPTP G KPDL++PG + SL GS QY LSGTS++ Sbjct: 288 AKIADFSSRGPTPDGFSKPDLLSPGVRINSLSHQGS-------------QYTSLSGTSMA 334 Query: 364 TPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRLGLKPNTAGSGEINMRFLL 415 TPIV+G AA L + PSL P+ VK+ + ++A + + G+G +++R +L Sbjct: 335 TPIVAGCAALLYEQNPSLNPQGVKSLMTKHALSINQNRDAQGAGILDIRKIL 386 >C0GDG5.1 C0GDG5_9FIRM Peptidase S8 and S53 subtilisin kexin sedolisin Length = 428 Score = 307 bits (787), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 175/422 (41%), Positives = 250/422 (59%), Gaps = 26/422 (6%) Query: 6 WVKQYASKMNRPLRNEIRKTFHSGKSSAAL---------------PVIVQF-KHKITPSR 49 W++ Y +K+ LR + + + + P+IVQ K+ + Sbjct: 8 WMRGYGTKICPELRKRVLDCYRPTRLTPCFLQKSIGKLTRRWKKFPIIVQLEKYAVHAIS 67 Query: 50 MLALHKHLGAHSFAIKHRIPMLNALSSRVSVNCLKRLCCWEGVHKVHLDHIKSISLDIAT 109 +L + G +K + +++A +S V+ L++L + V K+ D LD A+ Sbjct: 68 TYSLAEETGCK---LKKNLQVIDAFASEVTTEQLEKLLRNKNVKKIWHDGEVHAVLDAAS 124 Query: 110 PSIGAAAVQSKRGLTGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGH 169 P++GA + + G TGKGI IAVLDTG++ HPDL NR++ FKD +N PYDDNGH Sbjct: 125 PTVGAPGLWDE-GYTGKGIVIAVLDTGIYNHPDLQ---NRVLDFKDLINDDTNPYDDNGH 180 Query: 170 GTHITGDAAGNGWSSRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCIAEKKRLK 229 GTH+ G AA +G S GKY+GPAPEAG+VGVKVL+ +G G ST+I+GI WC+ K+RL Sbjct: 181 GTHVAGCAAASGSESNGKYKGPAPEAGLVGVKVLNKSGSGSLSTVIEGIGWCVQNKERLS 240 Query: 230 LRILSMSFGGPISTSCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGISPAAVT 289 +RI+++S G S RDD +CQA +A +AG+ V AAGNSGP +TI SP I P +T Sbjct: 241 IRIINLSLGSNAYQSHRDDPVCQAAVRAWEAGIVVCAAAGNSGPQPRTINSPAIEPGILT 300 Query: 290 VGAVDDRRTLPQKDDRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLARNFPYLR 349 VGA++DR P ++ + +SSRGPT G +KPD+ APG + +LR+PGS + + R Sbjct: 301 VGAINDRN--PNEEGFVAEFSSRGPTIDGLVKPDVCAPGVQITALRSPGSTIDKQNRDAR 358 Query: 350 IGKQYFVLSGTSVSTPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRL-GLKPNTAGSGE 408 I + LSGTS++TP+ +G AQLLQ P LTP Q+K L A L G N G+G Sbjct: 359 IDNWHTSLSGTSMATPVCAGVIAQLLQQNPFLTPDQIKNLLTETANPLTGYGENDQGAGV 418 Query: 409 IN 410 +N Sbjct: 419 VN 420 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,535,146,527 Number of extensions: 108276726 Number of successful extensions: 262442 Number of sequences better than 1.0e-05: 2802 Number of HSP's gapped: 257657 Number of HSP's successfully gapped: 2889 Length of query: 418 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 280 Effective length of database: 1,760,596,133 Effective search space: 492966917240 Effective search space used: 492966917240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)