BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0002.p (182 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q0SI11.1 Q0SI11_RHOSR Short chain dehydrogenase 173 5e-42 C1R5B4.1 C1R5B4_9ACTO Short-chain alcohol dehydrogenase 169 1e-40 C1QTL1.1 C1QTL1_9ACTO Short-chain alcohol dehydrogenase 166 7e-40 A0QMG5.1 A0QMG5_MYCA1 Short-chain dehydrogenase/reductase SDR 165 2e-39 Q08TM4.1 Q08TM4_STIAU Short-chain dehydrogenase/reductase SDR 145 1e-33 >Q0SI11.1 Q0SI11_RHOSR Short chain dehydrogenase Length = 279 Score = 173 bits (439), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 89/162 (54%), Positives = 102/162 (62%) Query: 1 VTKAALPVLRKQRSGHFIQISSVGGRVGGTPGLAAYQAAKFAVAGFSEVLHNEVKPLGIK 60 VT+AALP+LR+QR G F+Q SSVGGRVGGTPGL+AYQAAKFAV GFSEVL EVKP G+ Sbjct: 114 VTRAALPILREQRRGCFVQFSSVGGRVGGTPGLSAYQAAKFAVEGFSEVLAAEVKPFGVT 173 Query: 61 VTIVEPGAFRTDWGGASMSIPPVGEDYEQTVGAMNRYRISVNGKENGDPVRAAGIIAXXX 120 V IVEPGAFRTDW G+SM VG DYE TVGAMNRYR +G + GDP RAA II Sbjct: 174 VLIVEPGAFRTDWQGSSMKRSSVGPDYEATVGAMNRYRQDTDGTQPGDPARAARIIMDVV 233 Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKWADISMSTNY 162 +WAD+S S ++ Sbjct: 234 AAEDAPLRLLLGAGAVEMTEKSSRERAAEAVQWADVSRSADF 275 >C1R5B4.1 C1R5B4_9ACTO Short-chain alcohol dehydrogenase Length = 261 Score = 169 bits (427), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 88/162 (54%), Positives = 101/162 (62%) Query: 1 VTKAALPVLRKQRSGHFIQISSVGGRVGGTPGLAAYQAAKFAVAGFSEVLHNEVKPLGIK 60 VT+AALPVLR +RSG F+Q SSVGGRVGGTPG+ AYQ+AKFAV GFSEVL EV P G+K Sbjct: 93 VTRAALPVLRARRSGVFVQFSSVGGRVGGTPGMGAYQSAKFAVEGFSEVLAAEVAPFGVK 152 Query: 61 VTIVEPGAFRTDWGGASMSIPPVGEDYEQTVGAMNRYRISVNGKENGDPVRAAGIIAXXX 120 V IVEPGAFRTDW G+SM + PVG DYE TVGAMN YR + + GDPVRAA +I Sbjct: 153 VVIVEPGAFRTDWQGSSMELHPVGADYESTVGAMNAYRAENSDNQPGDPVRAARVIIDVV 212 Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKWADISMSTNY 162 KWAD S S ++ Sbjct: 213 GHDDPPRRLLLGAQAVTSALAAGEARAEETRKWADASASADF 254 >C1QTL1.1 C1QTL1_9ACTO Short-chain alcohol dehydrogenase Length = 279 Score = 166 bits (421), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 82/116 (70%), Positives = 92/116 (79%) Query: 1 VTKAALPVLRKQRSGHFIQISSVGGRVGGTPGLAAYQAAKFAVAGFSEVLHNEVKPLGIK 60 VT+AALPVLR QRSG F+Q SSVGGRVGGTPG+ AYQ+AKFAV GFSEVL EV P G+K Sbjct: 114 VTRAALPVLRAQRSGVFVQFSSVGGRVGGTPGMGAYQSAKFAVEGFSEVLAAEVAPFGVK 173 Query: 61 VTIVEPGAFRTDWGGASMSIPPVGEDYEQTVGAMNRYRISVNGKENGDPVRAAGII 116 V IVEPGAFRTDW G+SM + VG DYE TVGAMN YR + + GDPVRAA +I Sbjct: 174 VVIVEPGAFRTDWQGSSMELHAVGADYESTVGAMNAYRAENSDNQPGDPVRAARVI 229 >A0QMG5.1 A0QMG5_MYCA1 Short-chain dehydrogenase/reductase SDR Length = 288 Score = 165 bits (417), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 86/162 (53%), Positives = 100/162 (61%) Query: 1 VTKAALPVLRKQRSGHFIQISSVGGRVGGTPGLAAYQAAKFAVAGFSEVLHNEVKPLGIK 60 VTKAALPVL +QR+GH +QISSVGGRVGG+PG+AAYQA+KFAVAGFSE L EV PLGIK Sbjct: 114 VTKAALPVLHRQRAGHIVQISSVGGRVGGSPGIAAYQASKFAVAGFSEALAAEVAPLGIK 173 Query: 61 VTIVEPGAFRTDWGGASMSIPPVGEDYEQTVGAMNRYRISVNGKENGDPVRAAGIIAXXX 120 VTI EPGA RT+W G+SM++ V DYE TVGAMNR R N GDP RAA I Sbjct: 174 VTIAEPGALRTEWAGSSMAVATVSPDYESTVGAMNRRRAGFNRNAPGDPARAAHAIIEVV 233 Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKWADISMSTNY 162 +WA++S S +Y Sbjct: 234 IADDPPLRLLLGSDALSAALAKSHQQIAEANRWAELSKSIDY 275 >Q08TM4.1 Q08TM4_STIAU Short-chain dehydrogenase/reductase SDR Length = 317 Score = 145 bits (367), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 81/178 (45%), Positives = 101/178 (56%), Gaps = 1/178 (0%) Query: 1 VTKAALPVLRKQRSGHFIQISSVGGRVGGTPGLAAYQAAKFAVAGFSEVLHNEVKPLGIK 60 VT+AALP+LR QR GH IQISS+GGR G TPGLAAYQ+AK+AV GFSEVL EV LGI+ Sbjct: 137 VTRAALPILRAQREGHIIQISSIGGR-GSTPGLAAYQSAKWAVGGFSEVLAKEVGSLGIR 195 Query: 61 VTIVEPGAFRTDWGGASMSIPPVGEDYEQTVGAMNRYRISVNGKENGDPVRAAGIIAXXX 120 VT+VEPG RTDW G+SM + DY+ TVGAM YR GDP + A I Sbjct: 196 VTVVEPGGMRTDWAGSSMKTGDIQSDYQSTVGAMVAYRHENPDIMRGDPAKCAQAILQVA 255 Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKWADISMSTNYEGLSERVDNPVLQRIK 178 KW +S+ST+++GL+ ++PV + K Sbjct: 256 SEKEPPLRLLLGSDAVFLSGLIAEARAEEDAKWRALSLSTDFDGLAPFSESPVAKLAK 313 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 896,028,889 Number of extensions: 29650904 Number of successful extensions: 80011 Number of sequences better than 1.0e-05: 1668 Number of HSP's gapped: 84453 Number of HSP's successfully gapped: 1668 Length of query: 182 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 55 Effective length of database: 1,864,227,298 Effective search space: 102532501390 Effective search space used: 102532501390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 127 (53.5 bits)