BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0027.p (210 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDZ8.1 B3KDZ8_9BACI Conserved hypothetical membrane protein 286 1e-75 B1D5J6.1 B1D5J6_9BACL Putative transmembrane anti-sigma factor 211 5e-53 Q2B5B1.1 Q2B5B1_9BACI YbbM 163 1e-38 A6CNQ7.1 A6CNQ7_9BACI YbbM 161 4e-38 B7GIY6.1 B7GIY6_ANOFW Membrane-anchored regulator of SigW (Anti-... 160 4e-38 >B3KDZ8.1 B3KDZ8_9BACI Conserved hypothetical membrane protein Length = 204 Score = 286 bits (731), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 124/204 (60%), Positives = 169/204 (82%) Query: 7 MECKLAVSMMHDYLDDDLPELQQRELKEHLLSCTDCRAKFKELEQTDMLMFSLMHQNPVA 66 M+CKLAVS+MHDYLD+DL + QQ EL++HLL C DCR F++LE TDML+FSL H Sbjct: 1 MDCKLAVSLMHDYLDNDLSDPQQLELQKHLLDCADCRNYFEKLEHTDMLLFSLTHHATKP 60 Query: 67 SEDLVGRIMNSLPHVKKERAFITWIKRHPALTAASVFILVMLMSSVTFWNQDRQLVVRGS 126 S+DL RIM LP KK++A++ W+K HPA+TAA++F++VML S+++FW+QD QLVV+ Sbjct: 61 SDDLTERIMGMLPQPKKQKAWVKWVKNHPAITAAALFLVVMLFSTISFWSQDTQLVVKSD 120 Query: 127 DLEQVVIKGDTVIVPSGKTISGDLTVENGKTQVYGEVNGNVTVIDGSLYQASTAHISGQV 186 + E++VI+GDTVI+P + I+GD+TVENGKT+VYG+VNGNVTVIDG L+QASTAHI+GQV Sbjct: 121 EFEKLVIEGDTVIIPPDQIITGDITVENGKTKVYGQVNGNVTVIDGELFQASTAHIAGQV 180 Query: 187 KSIDQAVSWLWYKVTNMFSEVAYR 210 K ID+A+ W+WYK++ +F++ AYR Sbjct: 181 KDIDRAMDWIWYKISTLFTDAAYR 204 >B1D5J6.1 B1D5J6_9BACL Putative transmembrane anti-sigma factor Length = 210 Score = 211 bits (536), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 107/209 (51%), Positives = 138/209 (66%), Gaps = 7/209 (3%) Query: 7 MECKLAVSMMHDYLDDDLPELQQRELKEHLLSCTDCRAKFKELEQTDMLMF-SLMHQNPV 65 M C +AV MHDYLD DLP +ELK HL SC C A+F++LE+T+ +M +L H + Sbjct: 1 MNCNVAVVWMHDYLDGDLPREDAQELKNHLHSCPACLARFEQLEKTEAMMLGTLEHTVSI 60 Query: 66 ASED---LVGRIMNSLPH---VKKERAFITWIKRHPALTAASVFILVMLMSSVTFWNQDR 119 D L RIM LP K+ R + +I ++P LTAA++F+LVML S + W QD Sbjct: 61 PDYDKSALTDRIMAQLPKPAKTKERRGVLRFIYKYPGLTAAALFVLVMLGSFTSIWEQDS 120 Query: 120 QLVVRGSDLEQVVIKGDTVIVPSGKTISGDLTVENGKTQVYGEVNGNVTVIDGSLYQAST 179 +L+V G DL+QVVI G+TV VP G + G+L VENG V GEV GNVTVIDGSL AST Sbjct: 121 KLIVSGEDLQQVVINGNTVTVPEGAHVKGNLVVENGTADVKGEVEGNVTVIDGSLNLAST 180 Query: 180 AHISGQVKSIDQAVSWLWYKVTNMFSEVA 208 +I+GQ ++IDQA+ W WYKVT FS +A Sbjct: 181 GYIAGQSRTIDQALDWFWYKVTETFSGLA 209 >Q2B5B1.1 Q2B5B1_9BACI YbbM Length = 233 Score = 163 bits (412), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 75/191 (39%), Positives = 119/191 (62%), Gaps = 4/191 (2%) Query: 13 VSMMHDYLDDDLPELQQRELKEHLLSCTDCRAKFKELEQTDMLMFSLMHQNPVASEDLVG 72 V MH+YLD+++ +++L+EHL SC +CR+ F EL++ L+ S H A Sbjct: 29 VEYMHEYLDEEISAEHEQQLREHLSSCDECRSYFHELKRAIALVQSTSHIQ--APSGFTA 86 Query: 73 RIMNSLPHVKKERAFITWIKRHPALTAASVFILVMLMSSVTFWNQDRQLVVRGSDLEQVV 132 +M LP KK+ F W + HP LTAAS+F+++M+ S V+ WN+D V S ++ Sbjct: 87 GVMAKLPKEKKQVGFQRWFRHHPLLTAASLFLILMMGSVVSTWNKDHNFSV--SKQPNLI 144 Query: 133 IKGDTVIVPSGKTISGDLTVENGKTQVYGEVNGNVTVIDGSLYQASTAHISGQVKSIDQA 192 ++ DTVIVP G+ I GD+ V NGK ++ G+V+G+VTVI+G Y AS ++G +K +++ Sbjct: 145 VQNDTVIVPEGEVIKGDVIVRNGKIKIEGQVDGDVTVINGEQYMASAGQVTGDIKEVNEV 204 Query: 193 VSWLWYKVTNM 203 WLWY + ++ Sbjct: 205 FEWLWYHIKSL 215 >A6CNQ7.1 A6CNQ7_9BACI YbbM Length = 214 Score = 161 bits (407), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 80/208 (38%), Positives = 127/208 (61%), Gaps = 6/208 (2%) Query: 1 MKGLAPMECKLAVSMMHDYLDDDLPELQQRELKEHLLSCTDCRAKFKELEQTDMLMFSLM 60 MK L K +H+YLD+++ + + +LKEHL C +C+ F E++++ L+ S Sbjct: 1 MKPLKSCCSKEYEEYIHEYLDEEISQDHESKLKEHLQRCEECQQFFHEMKKSIALVQSTS 60 Query: 61 HQNPVASEDLVGRIMNSLPHVKKERAFITWIKRHPALTAASVFILVMLMSSVTFWNQDRQ 120 H A ED ++M +LP KK+ W++ HP +TAAS+FI++M+ S + WN+ + Sbjct: 61 HIQ--APEDFTSKVMANLPREKKKVGVQRWLRHHPLMTAASLFIVLMMGSLFSSWNESDE 118 Query: 121 L-VVRGSDLEQVVIKGDTVIVPSGKTISGDLTVENGKTQVYGEVNGNVTVIDGSLYQAST 179 + DL V++G TVI+P GK I G++TV+NG +V G VNG++TVI+G+ Y AS Sbjct: 119 FSFTKQPDL---VVQGHTVIIPEGKVIDGNITVKNGDVKVEGTVNGDITVINGNQYLASA 175 Query: 180 AHISGQVKSIDQAVSWLWYKVTNMFSEV 207 ++G++K ID A WLWY++ F E Sbjct: 176 GSVTGEIKEIDAAFEWLWYQIKKGFKET 203 >B7GIY6.1 B7GIY6_ANOFW Membrane-anchored regulator of SigW (Anti-sigma factor) Length = 207 Score = 160 bits (406), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 77/203 (37%), Positives = 119/203 (58%), Gaps = 5/203 (2%) Query: 7 MEC-KLAVSMMHDYLDDDLPELQQRELKEHLLSCTDCRAKFKELEQTDMLMFSLMHQNPV 65 M C K V MHDYLD D+P+ ++++L+ H+ C CR F LE+T + ++ H P Sbjct: 4 MPCSKQIVQWMHDYLDGDIPKEEEQQLRSHIQQCEACREHFYALEKTVAFVENVSHFTP- 62 Query: 66 ASEDLVGRIMNSLPHVKKERAFITWIKRHPALTAASVFILVMLMSSVTFWNQDRQLVVRG 125 S+D + ++M +LP K+ + W+ HP LTAAS+F L+ S W +D + Sbjct: 63 -SDDFIKKVMYNLPREKRRVRWKRWLTHHPFLTAASLFFLLSTSSLWAVWQEDNEFSF-- 119 Query: 126 SDLEQVVIKGDTVIVPSGKTISGDLTVENGKTQVYGEVNGNVTVIDGSLYQASTAHISGQ 185 S E ++++ TVIVP GK + GD+ V NG Q+ GEV GNVTVI+G Y AS ++GQ Sbjct: 120 SKQEHLIVQNHTVIVPRGKVVEGDIVVRNGDIQIEGEVRGNVTVINGHKYLASAGEVTGQ 179 Query: 186 VKSIDQAVSWLWYKVTNMFSEVA 208 ++ +++ W+WY V + +A Sbjct: 180 IEEVNEVFDWIWYNVKRVGYNIA 202 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,194,032,024 Number of extensions: 44970642 Number of successful extensions: 145796 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 145750 Number of HSP's successfully gapped: 26 Length of query: 210 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 80 Effective length of database: 1,835,964,253 Effective search space: 146877140240 Effective search space used: 146877140240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)