BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0014.p (444 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2JRR1.1 B2JRR1_BURP8 Putative enzyme of poly-gamma-glutamate bi... 208 9e-52 B9ZJ16.1 B9ZJ16_NATMA Putative enzyme of poly-gamma-glutamate bi... 188 8e-46 B8KIC0.1 B8KIC0_9GAMM Y4sH 182 5e-44 C4CHH2.1 C4CHH2_9CHLR Bacterial capsule synthesis protein PGA_cap 182 8e-44 Q1GMM5.1 Q1GMM5_SILST Putative enzyme of poly-gamma-glutamate bi... 171 1e-40 >B2JRR1.1 B2JRR1_BURP8 Putative enzyme of poly-gamma-glutamate biosynthesis (Capsule formation)-like protein Length = 448 Score = 208 bits (529), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 147/442 (33%), Positives = 203/442 (45%), Gaps = 40/442 (9%) Query: 1 MKFLVTGDALFSSSNLYKTMDPELLAL---LQGADEVFTNAEFVTP-RKNTAPAAGRGYQ 56 + L TGDA+ + + D + LAL L+ D TN E V P R A G Sbjct: 4 VSVLATGDAMLTQ-RISPNPDADFLALVEMLRKTDVTITNVEMVFPGRGRMASTTMHGIP 62 Query: 57 TSVRPKALDEFGHLNIRYVSFANNHTGDYGVEGLVDTIEEAEERGLIPLGVGMSLHEARK 116 V P+ L EF L I +NH DYGV GLV +IE E RGL GVG +L EAR Sbjct: 63 CGVEPELLSEFEWLGIDIYGMGHNHATDYGVSGLVASIEALEARGLTYAGVGRTLQEARA 122 Query: 117 PVFVDTADGRIAIITIDVTRSEVFAASNPGNGVPARPGVNPLRWSRTYVVNDQDFEALKE 176 P + D R+A I + + + A++P G RPG PLR +T+ + + F+ L+E Sbjct: 123 PRYFDAPGCRVAYIAAGSSNARLCLAADPSVGDVGRPGTAPLRVQKTHYIRKERFDELRE 182 Query: 177 ISERIGI--------APSMEIGKRIETYKSKSENHYEF----GSLFEGYLTFEKGDQSRV 224 I G+ AP + + +N Y+ G EG + F GD SRV Sbjct: 183 ILAEAGVNVSATGTTAPGIH-------FPYPDKNIYDGPPPGGFAVEG-VNFAPGDDSRV 234 Query: 225 KTTAHEQDQQEIFRTIRDARERSDFVFVSLHTHEGENENWYSDYPAEFIETFARGAVDAG 284 +T E+D I ++++A ++D VFV LH HEG W ++ PAEF++ A +DAG Sbjct: 235 QTDVLERDVDAIVASVKEASRQADLVFVGLHCHEGIQGRWNTEVPAEFLQPLAHQLIDAG 294 Query: 285 ASCVFGHGAHFTRGVELYQGQPIFYNLGSLFMEFEAGESIISPEMFTAYGYAENESPSTL 344 A VF HG H RGVE Y G+PI Y+LG+ E S E++ G + +P+ L Sbjct: 295 AHGVFAHGPHMLRGVEFYAGRPICYSLGNFIFNVETFSS-FPLEVYEQQGMPLSSTPADL 353 Query: 345 HKNRT--KDSEGNWQGFYSDRKFSENFLVSFDLSVEENRFDYELIPIDLRLTHPTVTKRG 402 T K+ W+ + F L D EL PI L P ++RG Sbjct: 354 FDVVTGYKNQPLFWESVVARFTFENGLLA-----------DSELHPITLGRDAPR-SRRG 401 Query: 403 LPXXXXXXXXXXXXXRLNAVSK 424 P RLN +SK Sbjct: 402 CPELASAEEGARILGRLNELSK 423 >B9ZJ16.1 B9ZJ16_NATMA Putative enzyme of poly-gamma-glutamate biosynthesis (Capsule formation)-like protein Length = 449 Score = 188 bits (478), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 137/447 (30%), Positives = 222/447 (49%), Gaps = 12/447 (2%) Query: 1 MKFLVTGDALFSSS-NLYKTMD-PELLALLQGADEVFTNAEFVTPRKNTAPAAGRG-YQT 57 + F GDA+ + ++Y+ EL+ +Q D N E + PAA G Sbjct: 6 LTFTAVGDAIVTQKFSVYEEESFNELIDQIQDQDVSVANLEVLLHNFEGYPAAQSGGTYM 65 Query: 58 SVRPKALDEFGHLNIRYVSFANNHTGDYGVEGLVDTIEEAEERGLIPLGVGMSLHEARKP 117 P+ DE S A NH GD+ G+ T++ EER + G+G +L +AR P Sbjct: 66 QAPPEIADELEWAGFNLFSAATNHAGDFSHGGMEATMQALEERNMSYAGMGRNLAQARAP 125 Query: 118 VFVDTADGRIAIITIDVTRSEVFAASNPGNGVPARPGVNPLRWSRTYVVNDQDFEALKEI 177 ++DT GR+A+I+ T + A + RPG++PL Y V ++ E L Sbjct: 126 TYLDTPKGRVALISACTTITTGTEAGLQRPDMQGRPGISPLHLQTRYTVPEEFHEELIHA 185 Query: 178 SERIGIAPSMEIGKRIETYKSKSENHYEFGSL---FEGYLTFEKGDQSRVKTTAHEQDQQ 234 S+++G+ +++ KR ++ E+ F L E + FE GD+ + +++D + Sbjct: 186 SKKLGLE-AIKDRKRELGFQVPGEDSDGFTFLNIGGETDIQFELGDRFDIHQEVNDEDAE 244 Query: 235 EIFRTIRDARERSDFVFVSLHTHEGENENWYSDYPAEFIETFARGAVDAGASCVFGHGAH 294 I + I+ A+ ++D+VF+SLH+HEG + D +F+E+FAR +DAGA GHG H Sbjct: 245 SITKQIQAAKRQADWVFISLHSHEGTGGSRNDDTVPQFLESFARNCIDAGADGFIGHGPH 304 Query: 295 FTRGVELYQGQPIFYNLGSLFMEFEAGESIISPEMFTAYGYAENES-PSTLHKNRTKDSE 353 RG+E+Y+G PIFY+LG+ FM+ E ++ + E++ Y +S P+ L R + E Sbjct: 305 VLRGIEIYRGAPIFYSLGNFFMQNETIPNLPA-EIYDRYDLDPYQSLPADLFDERIFNDE 363 Query: 354 GNWQGFYSDRKFSENFLVSFDLSVEENRFDYELIPIDLRLTHPTVTKRGLPXXXXXXXXX 413 QGF +DRKF E+ L + E+ EL+P+DL + +RG P Sbjct: 364 QQRQGFTADRKFWESVLPICEFG-EDGVESIELLPLDLGYER-SRPQRGRPMLAGPDVTD 421 Query: 414 XXXXRLNAVSKERYNTEIVCEGKFFNV 440 +N +S + Y TE +G V Sbjct: 422 HVFATVNELSSQ-YGTEFTEDGPVLRV 447 >B8KIC0.1 B8KIC0_9GAMM Y4sH Length = 463 Score = 182 bits (462), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 133/404 (32%), Positives = 203/404 (50%), Gaps = 29/404 (7%) Query: 5 VTGDALFSSSNLYKTMDPE---LLALLQGADEVFTNAEFVTPRKNTAP-AAGRGYQTSVR 60 +TGD++ + L +PE ++ L++GAD FTN E + P A G Sbjct: 38 LTGDSIITR-KLSVYDEPEYLQMIDLIRGADVAFTNLEVLFHDYEPYPMAVSGGTWMRAD 96 Query: 61 PKALDEFGHLNIRYVSFANNHTGDYGVEGLVDTIEEAEERGLIPLGVGMSLHEARKPVFV 120 P+ E ++ ANNHTGDYGVEG+ T E GL+ GVG SL EAR+ F+ Sbjct: 97 PELAAELSWAGFDLLARANNHTGDYGVEGMRLTTRHVREAGLMGAGVGESLAEAREAKFL 156 Query: 121 DTADGRIAIITIDVTRSEVFAASNPGNGVPARPGVNPLRWSRTYVVNDQDFEALKEISER 180 +T+ R+A+I++ + + A +P RPG+NPLR+ TY V + +F+ LK + Sbjct: 157 ETSKARVALISLSSSFAPHMGAGASRGDMPPRPGLNPLRYDTTYEVTENEFDRLKALGMS 216 Query: 181 IGIAPSMEIGKRIETYKSKSENHYEFGSLFEGYLTFEKGDQSRVKTTAHEQDQQEIFRTI 240 +G + + G+ K K + FG F+ D +RV+TTA+E+D I + Sbjct: 217 LGQFTNQD-GEPRGLDKDKPDEMELFGR------DFKLADNNRVRTTANEEDVAAIAAVV 269 Query: 241 RDARERSDFVFVSLHTHEGENENWYSDYPAEFIETFARGAVDAGASCVFGHGAHFTRGVE 300 +A+ +DFV V++H HEG+ + PAEF+ FAR +DAGA GHG H RG+E Sbjct: 270 NNAKRLADFVIVTIHAHEGQKRS----EPAEFLPVFARAMIDAGADVFVGHGPHALRGIE 325 Query: 301 LYQGQPIFYNLGSLFMEFEAGESIISPEMFTAYGYAENESPSTLHKNRTKDSEGNWQGFY 360 +Y G+PI Y+LG + E + + + Y N + + R K+ GF Sbjct: 326 IYDGKPIMYSLGDFIFQNETLLR-LPYDNYAHYDLGGNSHLADFNDKRYKNDSA---GFP 381 Query: 361 SDRKFSENFLVSFDLSVEENRFDYELIPIDLRLTHPTVTKRGLP 404 ++ + E + + V ENR EL+ + L HP GLP Sbjct: 382 ANPEIWEAVVA---VPVFENR---ELVELAL---HPITLGYGLP 416 >C4CHH2.1 C4CHH2_9CHLR Bacterial capsule synthesis protein PGA_cap Length = 438 Score = 182 bits (461), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 136/442 (30%), Positives = 215/442 (48%), Gaps = 23/442 (5%) Query: 1 MKFLVTGDALFSSSNLYKTMDP--ELLALLQGADEVFTNAEFVTPRKNTAPAAGRGYQTS 58 ++ +TGD++ + P +L +++ AD FTN E V PA G Sbjct: 7 LRLALTGDSIITRRFAAPPGSPFDQLRQIIREADVAFTNLEVVPNDFQGYPAYESGGSHL 66 Query: 59 VRPK-ALDEFGHLNIRYVSFANNHTGDYGVEGLVDTIEEAEERGLIPLGVGMSLHEARKP 117 P +DE + + A NH+ +Y + GL+ I+ E +G+ G+G +L EAR P Sbjct: 67 GAPSWVIDELVDMGFDLFATATNHSLNYSIAGLLALIDILERKGVAYAGIGRNLAEARMP 126 Query: 118 VFVDTADGRIAIITIDVTRSEVFAASNPGNGVPARPGVNPLRWSRTYVVNDQDFEALKEI 177 V++D+ G IA+I+ T + AS + RPG+NPLR+ Y V E L+ I Sbjct: 127 VYLDSPRGSIAMISCASTFAPGQEASEQRPDMLGRPGLNPLRYETIYDVLPDQLETLRRI 186 Query: 178 SERIGIAPSMEIGKRIE---TYKSKSENHYEFGSLFEGYLTFEKGDQSRVKTTAHEQDQQ 234 +E +G+ + +RI+ + S + + F F D+ V TT +D Sbjct: 187 AEELGL--ERQRLERIQLGFAFPPDSPDIFPFLD-----TNFRAADRVAVHTTPKAKDLD 239 Query: 235 EIFRTIRDARERSDFVFVSLHTHEGENENWYSDYPAEFIETFARGAVDAGASCVFGHGAH 294 I +R+AR R+DFV VSLH HE ++ + PA FI TFA +D GA V GHG H Sbjct: 240 AIATWVREARARADFVLVSLHAHEQGSDR---EEPAAFIRTFAHRMIDEGADVVVGHGPH 296 Query: 295 FTRGVELYQGQPIFYNLGSLFMEFEAGESIISPEMFTAYGYAENESPSTLHKNRTKDSEG 354 RG+E Y+G+PIFY+LG+ F + E I S + + + + +PS +++ R D Sbjct: 297 LLRGMECYRGKPIFYSLGNFFGQNELTLKIPS-DSYERFRIDPSRTPSEMYRIRHDD--- 352 Query: 355 NWQGFYSDRKFSENFLVSFDLSVEENRFDYELIPIDLRLTHPTVTKRGLPXXXXXXXXXX 414 + +GF +D ++ E + L+ E E++P+ L P+ +RG P Sbjct: 353 DRKGFPADSRYWETVVPICHLA-EGRVTKIEIVPVSLGHGEPS-HRRGKPRLATGAHAEA 410 Query: 415 XXXRLNAVSKERYNTEIVCEGK 436 R A+S E Y T I +G+ Sbjct: 411 ILKRFAALS-EPYGTRIDLDGE 431 >Q1GMM5.1 Q1GMM5_SILST Putative enzyme of poly-gamma-glutamate biosynthesis (Capsule formation)-like protein Length = 431 Score = 171 bits (434), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 129/427 (30%), Positives = 201/427 (47%), Gaps = 23/427 (5%) Query: 5 VTGDALFSSSNLYKTMDP---ELLALLQGADEVFTNAEFVTPRKNTAPAAGRGYQTSVRP 61 +TGD++ L T DP L L++G D FTN E + PA G P Sbjct: 6 LTGDSILQR-RLLSTSDPVIKPLFDLIRGCDAAFTNLEVLPNDYRGDPAFDSGGSHFGAP 64 Query: 62 K-ALDEFGHLNIRYVSFANNHTGDYGVEGLVDTIEEAEERGLIPLGVGMSLHEARKPVFV 120 LD+ S A NHT DY + GL +++ ++RGL G G L EAR+ ++ Sbjct: 65 SWVLDDLVEAGFGMFSTATNHTLDYSISGLEYGLDQLDKRGLCHAGAGRHLEEARRAAYL 124 Query: 121 DTADGRIAIITIDVTRSEVFAASNPGNGVPARPGVNPLRWSRTYVVNDQDFEALKEISER 180 T + I ++ T ++ A+ + RPG+NPL TY V + +KE++E Sbjct: 125 TTPNAAIGMVACGSTYTKGQEAARQTAAMQGRPGLNPLWPDSTYEVTAEQMAVVKEMAEG 184 Query: 181 IGIAPSMEIGKRIETYKS--KSENHYEFGSLFEGYLTFEKGDQSRVKTTAHEQDQQEIFR 238 +G+ +I RI T + E + F + F G+Q+R + +D I R Sbjct: 185 LGLEKFRQI--RISTGFAFEAPEGIFPFNG-----MNFRVGEQTRHVRHPNPKDLAAIIR 237 Query: 239 TIRDARERSDFVFVSLHTHEGENENWYSDYPAEFIETFARGAVDAGASCVFGHGAHFTRG 298 + +A+ SD VS+H HE +E D PA+FI FA +DAGA V GHG H RG Sbjct: 238 WVEEAKLASDIALVSIHAHEHADEK---DQPADFIVEFAHAVIDAGADLVVGHGPHLLRG 294 Query: 299 VELYQGQPIFYNLGSLFMEFEAGESIISPEMFTAYGYAENESPSTLHKNRTKDSEGNWQG 358 +E+Y+G+PIFY+LG+ + E + E + + + +P+ L+K RT D + +G Sbjct: 295 MEIYKGKPIFYSLGNFIGQNEMVRQLPG-ESYDRFHVDDRLTPTQLYKQRTLDDQ---KG 350 Query: 359 FYSDRKFSENFLVSFDLSVEENRFDYELIPIDLRLTHPTVTKRGLPXXXXXXXXXXXXXR 418 F SD ++ + +V + D E+ P+ L L +RG P R Sbjct: 351 FPSDERYWQT-VVPICHFEGDGLVDVEIHPVSLGLGEQR-HRRGRPRLAHGAEAESILNR 408 Query: 419 LNAVSKE 425 +++S E Sbjct: 409 FSSLSYE 415 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,693,246,641 Number of extensions: 116674976 Number of successful extensions: 255620 Number of sequences better than 1.0e-05: 58 Number of HSP's gapped: 255620 Number of HSP's successfully gapped: 65 Length of query: 444 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 305 Effective length of database: 1,751,175,118 Effective search space: 534108410990 Effective search space used: 534108410990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)