BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0002.p (335 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ54.1 B3KJ54_9BACI Arsenical pump membrane protein 485 e-135 Q2BBE9.1 Q2BBE9_9BACI YdfA 464 e-129 A6CHI8.1 A6CHI8_9BACI YdfA 458 e-127 P96678.1 YDFA_BACSU Putative arsenical pump membrane protein ydfA 456 e-126 Q65IV3.1 Q65IV3_BACLD YdfA 453 e-125 >B3KJ54.1 B3KJ54_9BACI Arsenical pump membrane protein Length = 431 Score = 485 bits (1248), Expect = e-135, Method: Compositional matrix adjust. Identities = 237/289 (82%), Positives = 261/289 (90%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVRAL+FDE+KVFPFIIA GFIADTTSLPLVVSNLVNIVSADFFG+TF+EY Sbjct: 119 LILTPIVLAMVRALNFDERKVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFGITFIEY 178 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A RML+P LFSL AS+LVLYLFFR+SIPRN+D +QLK P AI+D MF+LSW VL++LL Sbjct: 179 ASRMLVPNLFSLAASVLVLYLFFRKSIPRNYDGTQLKQPSTAIQDPAMFRLSWIVLSMLL 238 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 GYFV EF IPVS VAGV+AI FL+MARKSP V E++KGAPWAIVFFSIGMYVVVYG Sbjct: 239 IGYFVCEFAAIPVSIVAGVIAILFLLMARKSPAVQTKEVLKGAPWAIVFFSIGMYVVVYG 298 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 LRNAGLTD+LA VIQ+ ADQGLFAA++ MGFIAA+ISS+MNNLPTVMIDALAIDATHTS Sbjct: 299 LRNAGLTDLLAGVIQSAADQGLFAATMAMGFIAAMISSIMNNLPTVMIDALAIDATHTSA 358 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 IKEALIYANVIGSDLGPK TPIGSLATLLWLHVLS KG+KISWGTYFK Sbjct: 359 AIKEALIYANVIGSDLGPKITPIGSLATLLWLHVLSAKGVKISWGTYFK 407 >Q2BBE9.1 Q2BBE9_9BACI YdfA Length = 426 Score = 464 bits (1195), Expect = e-129, Method: Compositional matrix adjust. Identities = 226/289 (78%), Positives = 257/289 (88%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVR L FDEK VFPFIIA GFIADTTSLPLVVSNLVNIVSADFFG+ F+E+ Sbjct: 114 LILTPIVLAMVRNLKFDEKMVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFGIGFVEF 173 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A RM+IP FSL ASI+VLYL+FR+SIP+N+D S LK P A++D +MF+LSW VLA+LL Sbjct: 174 ASRMIIPNFFSLAASIIVLYLYFRKSIPKNYDLSHLKEPSAAVRDSKMFRLSWLVLAILL 233 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 GYF SEF+ +PVS +AGVVAIFFL+MAR+SP V+ ++KGAPWAIVFFSIGMYVVVYG Sbjct: 234 IGYFASEFIGLPVSIIAGVVAIFFLLMARRSPAVNTRTVIKGAPWAIVFFSIGMYVVVYG 293 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 LRN GLT+VLADVIQ ADQGLFAA++GMGFIAA++SSVMNN+PTVMIDALAI T TSG Sbjct: 294 LRNVGLTNVLADVIQMTADQGLFAATIGMGFIAAILSSVMNNMPTVMIDALAIADTDTSG 353 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 +I+EALIYANVIGSDLGPK TPIGSLATLLWLHVLS KG+KISWGTYFK Sbjct: 354 VIREALIYANVIGSDLGPKITPIGSLATLLWLHVLSLKGVKISWGTYFK 402 >A6CHI8.1 A6CHI8_9BACI YdfA Length = 437 Score = 458 bits (1178), Expect = e-127, Method: Compositional matrix adjust. Identities = 227/295 (76%), Positives = 259/295 (87%), Gaps = 6/295 (2%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVR L+F+EK VFPFIIA GFIADTTSLPLVVSNLVNIVSADFFG+ F+EY Sbjct: 119 LILTPIVLAMVRNLNFNEKMVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFGIGFVEY 178 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A RM++P F+L +SI+VLYLFFR+SIP+N+D S LK P EAIKD +MFKLSW VLAVLL Sbjct: 179 ASRMIVPNFFALLSSIIVLYLFFRKSIPKNYDLSDLKEPAEAIKDHKMFKLSWVVLAVLL 238 Query: 143 AGYFVSEFLNI------PVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGM 196 GYF SEF+ I PVS +AG+VAIFFL+MAR+SP V+ ++KGAPWAIVFFSIGM Sbjct: 239 VGYFASEFIEINDEMTLPVSFIAGIVAIFFLLMARRSPAVNTKTVIKGAPWAIVFFSIGM 298 Query: 197 YVVVYGLRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAID 256 YVVVYGLRNAGLTDVLA VIQA ADQGLF+A++ MGFIAA++SSVMNN+PTVMIDALAI Sbjct: 299 YVVVYGLRNAGLTDVLAGVIQAAADQGLFSATISMGFIAAILSSVMNNMPTVMIDALAIA 358 Query: 257 ATHTSGLIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 T+T+G ++EALIYANVIGSDLGPK TPIGSLATLLWLHVLS KG+KISWGTYFK Sbjct: 359 ETNTTGTVREALIYANVIGSDLGPKITPIGSLATLLWLHVLSLKGVKISWGTYFK 413 >P96678.1 YDFA_BACSU Putative arsenical pump membrane protein ydfA Length = 435 Score = 456 bits (1174), Expect = e-126, Method: Compositional matrix adjust. Identities = 221/289 (76%), Positives = 256/289 (88%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVRAL F+EK VFPFIIA GFIADTTSLP VVSNLVNIVSAD+F +TF++Y Sbjct: 119 LILTPIVLAMVRALHFNEKLVFPFIIASGFIADTTSLPFVVSNLVNIVSADYFHITFIDY 178 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A RM++P LFSL ASI+VLYLFFR+SIP+ +D +++K P EAIKD+ MF+LSW++L +LL Sbjct: 179 ASRMVVPYLFSLLASIIVLYLFFRKSIPKRYDLTEVKKPVEAIKDQNMFRLSWYILGLLL 238 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 GYF SEF +IPVS VAG +AI FLI A+KSP VH ++VK APWAIVFFSIGMYVVVYG Sbjct: 239 IGYFASEFFSIPVSVVAGSIAIIFLIAAQKSPAVHTKKVVKEAPWAIVFFSIGMYVVVYG 298 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 +RNAGLTDVL+DVIQA ADQGLFA ++GMGFIAA++SS+MNNLPTVMIDALAI T T G Sbjct: 299 VRNAGLTDVLSDVIQAAADQGLFAGTIGMGFIAAILSSIMNNLPTVMIDALAIAGTDTHG 358 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 +++EALIYANVIGSDLGPK TPIGSLATLLWLHVLS KG+KISWGTYFK Sbjct: 359 MMREALIYANVIGSDLGPKITPIGSLATLLWLHVLSHKGMKISWGTYFK 407 >Q65IV3.1 Q65IV3_BACLD YdfA Length = 431 Score = 453 bits (1166), Expect = e-125, Method: Compositional matrix adjust. Identities = 218/289 (75%), Positives = 257/289 (88%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVRAL+F EK VFPFIIA GFIADTTSLPLVVSNLVNIVSADFF + F+EY Sbjct: 118 LILTPIVLAMVRALNFQEKLVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFNIGFIEY 177 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A RM++P LFSL ASI+VL+LFFR+SIPR++D + +K P EAI+D+++F+LSW +LAVLL Sbjct: 178 ATRMIVPNLFSLIASIIVLFLFFRKSIPRHYDMTNVKQPAEAIRDRKIFRLSWLILAVLL 237 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 AGYF+SE +IPVS +AGV+A+ FLI A+ SP V +++KGAPWAIVFFSIGMYVVVYG Sbjct: 238 AGYFISELFSIPVSIIAGVIAVIFLITAKNSPAVQTKKVLKGAPWAIVFFSIGMYVVVYG 297 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 LRNAGLTDVL+ VIQ V+DQGLFA ++GMGFIAA++SS+MNN+PTVMIDALAI T T G Sbjct: 298 LRNAGLTDVLSQVIQTVSDQGLFAGTMGMGFIAAILSSLMNNMPTVMIDALAIQNTDTHG 357 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 +++EALIYANVIGSDLGPK TPIGSLATLLWLHVLS KG+KISWGTYFK Sbjct: 358 ILREALIYANVIGSDLGPKITPIGSLATLLWLHVLSLKGVKISWGTYFK 406 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,579,400,876 Number of extensions: 61543372 Number of successful extensions: 279421 Number of sequences better than 1.0e-05: 931 Number of HSP's gapped: 278893 Number of HSP's successfully gapped: 935 Length of query: 335 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 199 Effective length of database: 1,779,438,163 Effective search space: 354108194437 Effective search space used: 354108194437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)