BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0045.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKE8.1 B3KKE8_9BACI Preprotein translocase secY subunit 677 0.0 C0ZIK0.1 C0ZIK0_BREBN Preprotein translocase SecY subunit 524 e-147 P38375.2 SECY_BACHD Preprotein translocase subunit secY 509 e-142 B3ZH02.1 B3ZH02_BACCE Preprotein translocase secY subunit 500 e-140 Q81VR0.1 Q81VR0_BACAN Preprotein translocase secY subunit 499 e-139 >B3KKE8.1 B3KKE8_9BACI Preprotein translocase secY subunit Length = 432 Score = 677 bits (1747), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/432 (78%), Positives = 375/432 (86%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MFKTLKNIW +EDLRK+ILFTLFVLII+RIGSFVPVPGVNK+V TNQ G L GLLNT Sbjct: 1 MFKTLKNIWRVEDLRKRILFTLFVLIIFRIGSFVPVPGVNKDVFTQTNQAGNELFGLLNT 60 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 FSGGAL FSIFA+SI+PYIT+SIIVQLLSMDV+PKFAEW KQGEHGKKQLAQITRYGTV Sbjct: 61 FSGGALSQFSIFALSIFPYITSSIIVQLLSMDVIPKFAEWNKQGEHGKKQLAQITRYGTV 120 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 +LGLIQAF SIGFNR+YG EM+P ATF D+++IAI+LTAGTSFLMWLGEQITE+GIGNG Sbjct: 121 ILGLIQAFGMSIGFNRLYGAEMVPGATFIDFVVIAIVLTAGTSFLMWLGEQITERGIGNG 180 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQPDQXXXXXXXXXXXXXXXXXXXXXXXXXQQGIR 240 ISI+IFAGI A IP +I TT +S+FIQ Q QQGIR Sbjct: 181 ISIIIFAGIAAGIPMHIRTTIESNFIQDGQVFMNILKGVIILLILVLIITGVVFIQQGIR 240 Query: 241 KIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFWSTHEWAKW 300 KIPVQYAKRVVGNKMYGGQNTHIPLKIN AGVIPVIFA+SLLQFP++++SFW+ WAKW Sbjct: 241 KIPVQYAKRVVGNKMYGGQNTHIPLKINGAGVIPVIFAMSLLQFPVILASFWAHQNWAKW 300 Query: 301 VTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKATEKY 360 +T NLA+D PLGMVLYV+MIIGFTFFYTFVQMNP QMADNMKKNGGY+PGIRPGKATEKY Sbjct: 301 ITANLAYDSPLGMVLYVLMIIGFTFFYTFVQMNPNQMADNMKKNGGYVPGIRPGKATEKY 360 Query: 361 LTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQIESQ 420 LTRVM+RLTM+GALFLAVIS+LPV FGSL+GLPR VQIGGT+LLIVVGVALDTMKQIESQ Sbjct: 361 LTRVMTRLTMTGALFLAVISVLPVFFGSLAGLPRTVQIGGTSLLIVVGVALDTMKQIESQ 420 Query: 421 LIKRHYKGFINK 432 LIKRHYKGFINK Sbjct: 421 LIKRHYKGFINK 432 >C0ZIK0.1 C0ZIK0_BREBN Preprotein translocase SecY subunit Length = 431 Score = 524 bits (1350), Expect = e-147, Method: Compositional matrix adjust. Identities = 264/435 (60%), Positives = 331/435 (76%), Gaps = 10/435 (2%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLE-STNQGGEALMGLLN 59 M +L NI+ I DLR+KILFTL +L+++RIGS+VPVP VN + + +TNQ L+GLLN Sbjct: 1 MLASLTNIFKISDLRRKILFTLMMLVVFRIGSYVPVPNVNVALFQQNTNQ----LLGLLN 56 Query: 60 TFSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGT 119 TFSGGAL NFSIFA+ I PYITASII+QL+SMDVVPK +WA++GE G++++A +TRY T Sbjct: 57 TFSGGALHNFSIFAMGIMPYITASIIMQLMSMDVVPKLTQWAREGEVGRRKIATVTRYAT 116 Query: 120 VVLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGN 179 ++LGLIQ+ +IGFN + ++ + + + Y LIA+ LTAGT+F+MW+GEQITEKGIGN Sbjct: 117 IILGLIQSVGMTIGFNNM-APGLLNDTSVSSYALIALTLTAGTAFMMWMGEQITEKGIGN 175 Query: 180 GISILIFAGIVAAIPGYITTTAQSSFIQPDQXXXXXXXXXXXXXXXXXXXXX-XXXXQQG 238 GISILI GIVA IP ITT + F P Q QQG Sbjct: 176 GISILIVGGIVANIPQGITTIYTTQFADPSQNVFFSVVKVLLILLVILAIIVGVIFMQQG 235 Query: 239 IRKIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFW---STH 295 +RKIPVQYAKRVVG KMYGGQ+THIPLKIN+AGVIPVIFAVSL+ FP I+ FW S Sbjct: 236 LRKIPVQYAKRVVGRKMYGGQSTHIPLKINSAGVIPVIFAVSLVMFPSTIAQFWVDASGQ 295 Query: 296 EWAKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGK 355 +A W+ N +KPLGM LY I+I+GFT+FYTFVQ+NP QMA+NM+KNGGYIPGIRPGK Sbjct: 296 GFANWIYQNFQVNKPLGMTLYAILILGFTYFYTFVQINPVQMAENMRKNGGYIPGIRPGK 355 Query: 356 ATEKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMK 415 TE ++TR ++RLT++GALFL +I+ILP FGSL+ LP+ + IGGT+LLIVVGV+LDTMK Sbjct: 356 NTEVFITRTLNRLTLAGALFLMIIAILPFFFGSLANLPQSIYIGGTSLLIVVGVSLDTMK 415 Query: 416 QIESQLIKRHYKGFI 430 QIESQ+IKRHY+GFI Sbjct: 416 QIESQMIKRHYQGFI 430 >P38375.2 SECY_BACHD Preprotein translocase subunit secY Length = 430 Score = 509 bits (1311), Expect = e-142, Method: Compositional matrix adjust. Identities = 256/434 (58%), Positives = 322/434 (74%), Gaps = 7/434 (1%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+T+ NI+ + DLR+K++FTL +LI++RIGSF+PVPG N+EVL+ +Q G LNT Sbjct: 1 MFRTISNIFRVGDLRRKVIFTLLMLIVFRIGSFIPVPGTNREVLDFVDQANA--FGFLNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGAL NFSIFA+ I PYITASI++QLL MDVVPKFAEWAK+GE G+++LAQ TRYGT+ Sbjct: 59 FGGGALGNFSIFAMGIMPYITASIVMQLLQMDVVPKFAEWAKEGEAGRRKLAQFTRYGTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VLG IQA S+GFN + +IPN + + YL IA++LTAGT+FLMWLGEQIT KG+GNG Sbjct: 119 VLGFIQALGMSVGFNNFF-PGLIPNPSVSVYLFIALVLTAGTAFLMWLGEQITAKGVGNG 177 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQP--DQXXXXXXXXXXXXXXXXXXXXXXXXXQQG 238 ISI+IFAGI A IP + S+ IQ +Q QQ Sbjct: 178 ISIIIFAGIAAGIPNGLNLI-YSTRIQDAGEQLFLNIVVILLLALAILAIIVGVIFVQQA 236 Query: 239 IRKIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISS-FWSTHEW 297 +RKIPVQYAKR+VG GGQ+TH+PLK+NAAGVIPVIFA+SL FP ++ F S H Sbjct: 237 LRKIPVQYAKRLVGRNPVGGQSTHLPLKVNAAGVIPVIFALSLFIFPPTVAGLFGSDHPV 296 Query: 298 AKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKAT 357 A WV + P+GM +Y ++IIGFT+FYTF+Q+NP++MA+N+KK GGYIPGIRPGKAT Sbjct: 297 AAWVIETFDYTHPIGMAVYALLIIGFTYFYTFIQVNPERMAENLKKQGGYIPGIRPGKAT 356 Query: 358 EKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQI 417 + Y+TR++ RLT G+LFLAV++ILPV F + LP+ +QIGGT LLIVVGVALDTMKQI Sbjct: 357 QTYITRILYRLTFVGSLFLAVVAILPVFFIKFADLPQAIQIGGTGLLIVVGVALDTMKQI 416 Query: 418 ESQLIKRHYKGFIN 431 E+QLIKR YKGFI Sbjct: 417 EAQLIKRSYKGFIK 430 >B3ZH02.1 B3ZH02_BACCE Preprotein translocase secY subunit Length = 433 Score = 500 bits (1288), Expect = e-140, Method: Compositional matrix adjust. Identities = 249/435 (57%), Positives = 319/435 (73%), Gaps = 5/435 (1%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+T+ N + ++R+KILFTL +LI++RIG+F+PVP N +VL++ +Q +G+LNT Sbjct: 1 MFRTISNFMRVAEIRRKILFTLAMLIVFRIGTFIPVPFTNGDVLKAQDQLNA--LGILNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGALKNFSIFA+ I PYITASIIVQLL MDVVPKF+EWAKQGE G+++L Q TRY T+ Sbjct: 59 FGGGALKNFSIFAMGIMPYITASIIVQLLQMDVVPKFSEWAKQGEMGRRKLTQFTRYFTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VL IQ F SIGFN + G ++I N ++ YL IA +LTAGT+FLMWLGEQIT KG+GNG Sbjct: 119 VLAFIQGFGMSIGFNGMVGGQLILNPGWSTYLYIATVLTAGTAFLMWLGEQITAKGVGNG 178 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQ-PDQXXXXXXXXXXXXXXXXXXXXXXXXXQQGI 239 ISILIF GI AAIP I+ Q F DQ QQ + Sbjct: 179 ISILIFGGIAAAIPSVISQVYQQQFQNIGDQLFMSIVKVALILLAVLAVIVGVIFIQQAV 238 Query: 240 RKIPVQYAKRVVG-NKMYGG-QNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFWSTHEW 297 RKIP+QYAKRV G N Y G QNTH+PLK+N+AGVIPVIFAVS L P I+ F+ H+ Sbjct: 239 RKIPIQYAKRVTGGNSGYAGAQNTHLPLKVNSAGVIPVIFAVSFLITPPTIAQFFPKHDV 298 Query: 298 AKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKAT 357 ++W+ N + P+GM++YV +I+ FT+FY FVQ+NP+QM++N+ K GGY+PGIRPGK T Sbjct: 299 SQWIIANFNYSHPVGMIIYVALIVAFTYFYAFVQVNPEQMSENLNKQGGYVPGIRPGKNT 358 Query: 358 EKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQI 417 E+YLT+++ RLT G++FLA I+ILPV+F L LP QIGGT++LIVVGVAL+TMKQ+ Sbjct: 359 EQYLTKILYRLTFVGSIFLAAIAILPVIFTKLGNLPPSAQIGGTSMLIVVGVALETMKQL 418 Query: 418 ESQLIKRHYKGFINK 432 ESQL+KRHYKGFI + Sbjct: 419 ESQLVKRHYKGFIKQ 433 >Q81VR0.1 Q81VR0_BACAN Preprotein translocase secY subunit Length = 433 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 249/435 (57%), Positives = 319/435 (73%), Gaps = 5/435 (1%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+T+ N + ++R+KILFTL +LI++RIG+F+PVP N +VL++ +Q +G+LNT Sbjct: 1 MFRTISNFMRVAEIRRKILFTLAMLIVFRIGTFIPVPFTNGDVLKAQDQLNA--LGILNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGALKNFSIFA+ I PYITASIIVQLL MDVVPKF+EWAKQGE G+++L Q TRY T+ Sbjct: 59 FGGGALKNFSIFAMGIMPYITASIIVQLLQMDVVPKFSEWAKQGEMGRRKLTQFTRYFTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VL IQ F SIGFN + G ++I N ++ YL IA +LTAGT+FLMWLGEQIT KG+GNG Sbjct: 119 VLAFIQGFGMSIGFNGMVGGQLILNPGWSTYLYIATVLTAGTAFLMWLGEQITAKGVGNG 178 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQ-PDQXXXXXXXXXXXXXXXXXXXXXXXXXQQGI 239 ISILIF GI AAIP I+ Q F DQ QQ + Sbjct: 179 ISILIFGGIAAAIPSVISQVYQQQFQNIGDQLFMSIVKVALILLAVLAVIVGVIFIQQAV 238 Query: 240 RKIPVQYAKRVVG-NKMYGG-QNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFWSTHEW 297 RKIP+QYAKRV G N Y G QNTH+PLK+N+AGVIPVIFAVS L P I+ F+ H+ Sbjct: 239 RKIPIQYAKRVTGGNGGYAGAQNTHLPLKVNSAGVIPVIFAVSFLITPPTIAQFFPKHDV 298 Query: 298 AKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKAT 357 ++W+ N + P+GM++YV +I+ FT+FY FVQ+NP+QM++N+ K GGY+PGIRPGK T Sbjct: 299 SQWIIANFNYSHPVGMIIYVALIVAFTYFYAFVQVNPEQMSENLNKQGGYVPGIRPGKNT 358 Query: 358 EKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQI 417 E+YLT+++ RLT G++FLA I+ILPV+F L LP QIGGT++LIVVGVAL+TMKQ+ Sbjct: 359 EQYLTKILYRLTFVGSIFLAAIAILPVIFTKLGNLPPSAQIGGTSMLIVVGVALETMKQL 418 Query: 418 ESQLIKRHYKGFINK 432 ESQL+KRHYKGFI + Sbjct: 419 ESQLVKRHYKGFIKQ 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.141 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,362,476,649 Number of extensions: 93362181 Number of successful extensions: 334091 Number of sequences better than 1.0e-05: 1905 Number of HSP's gapped: 325078 Number of HSP's successfully gapped: 2885 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)