BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0012.p (230 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLN2.1 B3KLN2_9BACI Adenylylsulfate reductase, thioredoxin dep... 422 e-116 B1DJU0.1 B1DJU0_9BACL Adenylylsulfate reductase, thioredoxin dep... 395 e-108 C0Z9C2.1 C0Z9C2_BREBN Probable phosphoadenosine phosphosulfate r... 296 9e-79 C1PRF8.1 C1PRF8_ALIAC Phosphoadenylylsulfate reductase (Thioredo... 286 7e-76 B7DNJ2.1 B7DNJ2_9BACL Adenylylsulfate reductase, thioredoxin dep... 286 1e-75 >B3KLN2.1 B3KLN2_9BACI Adenylylsulfate reductase, thioredoxin dependent EC=1.8.4.8 Length = 230 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 191/230 (83%), Positives = 215/230 (93%) Query: 1 MNLLEKEDLIRVKAEELEHATPEEIIRWAVETFPNITFACSFGAEDVVLVDMLQKVSPST 60 MNLLEKEDLIRV+AEELEHA+ EE+I +A++TFPNITFACSFGAEDVVLVDM+QK+SPST Sbjct: 1 MNLLEKEDLIRVQAEELEHASAEEVIEFAIKTFPNITFACSFGAEDVVLVDMIQKISPST 60 Query: 61 DVFYLDTDFHFKETYETRDIMADKYGMEFVRVSPQITPAEQAVQYGEALWSTDPNQCCAI 120 D+FYLDTDFHFKETYETRD MA++YG+EFVRVSP ITP EQA Q+GEALWS PN+CC I Sbjct: 61 DIFYLDTDFHFKETYETRDRMAERYGLEFVRVSPLITPEEQAAQHGEALWSVSPNECCNI 120 Query: 121 RKVEPLTRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSYI 180 RKVEPLTRILSQY+AWITGIRRDQAPTRAN+KK+EYD KFGLVKFNPIA WTSEDVW+YI Sbjct: 121 RKVEPLTRILSQYEAWITGIRRDQAPTRANSKKIEYDTKFGLVKFNPIAGWTSEDVWNYI 180 Query: 181 RENDVVYNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLHK 230 R N+++YNPLHD+N+PSIGCE CTR VMPGEDPRAGRWSG +KTECGLHK Sbjct: 181 RTNEIIYNPLHDQNYPSIGCEYCTRQVMPGEDPRAGRWSGHEKTECGLHK 230 >B1DJU0.1 B1DJU0_9BACL Adenylylsulfate reductase, thioredoxin dependent EC=1.8.4.8 Length = 231 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 184/231 (79%), Positives = 205/231 (88%), Gaps = 1/231 (0%) Query: 1 MNLLEKEDLIRVKAEELEHATPEEIIRWAVETFPNITFACSFGAEDVVLVDMLQKVSPST 60 MNL EKE L++ KAEELE+ATP+EI+ +AVE FPNITFACSFGAEDVVLVDMLQKVSP T Sbjct: 1 MNLFEKEALVKQKAEELENATPQEILAYAVEAFPNITFACSFGAEDVVLVDMLQKVSPKT 60 Query: 61 DVFYLDTDFHFKETYETRDIMADKY-GMEFVRVSPQITPAEQAVQYGEALWSTDPNQCCA 119 D+FYLDTDFHFKETYETRD M ++Y G+ FV V P ITP EQA ++G LW +D N CC Sbjct: 61 DIFYLDTDFHFKETYETRDRMVERYPGIPFVEVKPLITPEEQAERHGAELWKSDANACCN 120 Query: 120 IRKVEPLTRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSY 179 IRKVEPLTRILS+YDAWITGIRRDQAPTRANAKKVEYD KFGL+KFNPIA WT+EDVW+Y Sbjct: 121 IRKVEPLTRILSKYDAWITGIRRDQAPTRANAKKVEYDTKFGLIKFNPIAGWTTEDVWNY 180 Query: 180 IRENDVVYNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLHK 230 IRENDV+YNPLHD+N+PSIGCE CTR VMPGEDPRAGRWSG +KTECGLHK Sbjct: 181 IRENDVIYNPLHDQNYPSIGCEYCTRQVMPGEDPRAGRWSGQEKTECGLHK 231 >C0Z9C2.1 C0Z9C2_BREBN Probable phosphoadenosine phosphosulfate reductase EC=1.8.4.8 Length = 233 Score = 296 bits (758), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 134/218 (61%), Positives = 167/218 (76%), Gaps = 1/218 (0%) Query: 14 AEELEHATPEEIIRWAVETFP-NITFACSFGAEDVVLVDMLQKVSPSTDVFYLDTDFHFK 72 A LE P++++ AVE + ++ A SFGAEDVVL+DML K++P+ VFYLDT+ HF Sbjct: 16 ASRLETQAPQDVLVNAVENYAASLILAASFGAEDVVLIDMLHKLAPTIPVFYLDTNIHFA 75 Query: 73 ETYETRDIMADKYGMEFVRVSPQITPAEQAVQYGEALWSTDPNQCCAIRKVEPLTRILSQ 132 ETY+TRD + +YG F++V PQ+T AEQA +G+ LW +PN CC IRKVEPL R LS Sbjct: 76 ETYDTRDKLQVRYGTTFIQVQPQVTLAEQAETHGDKLWEREPNLCCEIRKVEPLKRALSN 135 Query: 133 YDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSYIRENDVVYNPLHD 192 Y AWITGIRR+Q+PTR N KKVE+D KF LVKFNP+A+WT VW YI +DV YNPLHD Sbjct: 136 YQAWITGIRREQSPTRVNTKKVEWDEKFNLVKFNPLADWTEAQVWEYIHAHDVPYNPLHD 195 Query: 193 RNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLHK 230 RN+PSIGC CTRAV+PG+D R+GRW+G +KTECGLHK Sbjct: 196 RNYPSIGCRVCTRAVLPGQDSRSGRWAGFEKTECGLHK 233 >C1PRF8.1 C1PRF8_ALIAC Phosphoadenylylsulfate reductase (Thioredoxin) EC=1.8.4.8 Length = 234 Score = 286 bits (733), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 138/227 (60%), Positives = 163/227 (71%), Gaps = 1/227 (0%) Query: 4 LEKEDLIRVKAEELEHATPEEIIRWAVETFPNITFACSFGAEDVVLVDMLQKVSPSTDVF 63 LE+ I A E A PE+I+R A+ PNITFACSFGAED+V++DML + DVF Sbjct: 5 LEEAAWIEELAATYEEARPEDILREALRRVPNITFACSFGAEDMVILDMLMSIDQQADVF 64 Query: 64 YLDTDFHFKETYETRDIMADKYGM-EFVRVSPQITPAEQAVQYGEALWSTDPNQCCAIRK 122 YLDT+ F ETYE + KYG+ +V P++T EQA QYG+ALWS DPN CC IRK Sbjct: 65 YLDTNVLFPETYELIERAVAKYGIPNLHQVLPKLTLEEQAAQYGDALWSRDPNLCCKIRK 124 Query: 123 VEPLTRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSYIRE 182 VEPL L+ YD WITGIRR+QAPTR NAK E+D KF +VK NP+ WT +DVW+YI E Sbjct: 125 VEPLAAFLAGYDGWITGIRREQAPTRRNAKVFEWDAKFNIVKVNPLVRWTEKDVWNYIVE 184 Query: 183 NDVVYNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLH 229 V YNPLHDRN+PSIGC CTR V PGEDPR+GRW+G DKTECGLH Sbjct: 185 RGVPYNPLHDRNYPSIGCLHCTRPVKPGEDPRSGRWAGFDKTECGLH 231 >B7DNJ2.1 B7DNJ2_9BACL Adenylylsulfate reductase, thioredoxin dependent EC=1.8.4.8 Length = 234 Score = 286 bits (731), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 138/227 (60%), Positives = 163/227 (71%), Gaps = 1/227 (0%) Query: 4 LEKEDLIRVKAEELEHATPEEIIRWAVETFPNITFACSFGAEDVVLVDMLQKVSPSTDVF 63 LE+ I A E A PE+I+R A+ PNITFACSFGAED+V++DML + DVF Sbjct: 5 LEEAAWIEELAATYEEARPEDILREALRRVPNITFACSFGAEDMVILDMLMSIDTQADVF 64 Query: 64 YLDTDFHFKETYETRDIMADKYGM-EFVRVSPQITPAEQAVQYGEALWSTDPNQCCAIRK 122 YLDT+ F ETYE + KYG+ +V P++T EQA QYG+ALWS DPN CC IRK Sbjct: 65 YLDTNVLFPETYELIERAIAKYGIPNLHQVLPKLTLEEQAAQYGDALWSRDPNLCCKIRK 124 Query: 123 VEPLTRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSYIRE 182 VEPL L+ YD WITGIRR+QAPTR NAK E+D KF +VK NP+ WT +DVW+YI E Sbjct: 125 VEPLADFLAGYDGWITGIRREQAPTRRNAKVFEWDAKFNIVKVNPLVRWTEKDVWNYIVE 184 Query: 183 NDVVYNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLH 229 V YNPLHDRN+PSIGC CTR V PGEDPR+GRW+G DKTECGLH Sbjct: 185 RGVPYNPLHDRNYPSIGCLHCTRPVKPGEDPRSGRWAGFDKTECGLH 231 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.134 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,510,189,022 Number of extensions: 61735481 Number of successful extensions: 137402 Number of sequences better than 1.0e-05: 1307 Number of HSP's gapped: 135754 Number of HSP's successfully gapped: 1307 Length of query: 230 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 99 Effective length of database: 1,826,543,238 Effective search space: 180827780562 Effective search space used: 180827780562 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)