BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0010.p (248 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLN4.1 B3KLN4_9BACI N-acetylmuramoyl-L-alanine amidase CwlD EC... 363 6e-99 B1DJT8.1 B1DJT8_9BACL N-acetylmuramoyl-L-alanine amidase CwlD 291 3e-77 C3BER6.1 C3BER6_9BACI Germination-specific N-acetylmuramoyl-L-al... 233 8e-60 C3AY75.1 C3AY75_BACMY Germination-specific N-acetylmuramoyl-L-al... 232 2e-59 C3AGE3.1 C3AGE3_BACMY Germination-specific N-acetylmuramoyl-L-al... 232 2e-59 >B3KLN4.1 B3KLN4_9BACI N-acetylmuramoyl-L-alanine amidase CwlD EC=3.5.1.28 Length = 257 Score = 363 bits (933), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 165/243 (67%), Positives = 209/243 (86%) Query: 1 MSGRKQGKVSVWISWSSIKKGMLGAVLLAVMIGIIAYDMPTAKTLNYWSLPLSGKVIAID 60 MS R+ KV+VWIS IK+ ++GAVLLA++I ++ Y++P+AKT N WSLPL+GKVIA+D Sbjct: 1 MSRRRTDKVTVWISLKRIKQTLIGAVLLALLIIVVTYEIPSAKTSNTWSLPLAGKVIALD 60 Query: 61 AGHGGPDGGAVSREGLIEKDINLSVSLYLRDYLQQAGAIVVMTREGDYDLARQDTKGYAK 120 AGHGGPDGGAVSR+G+IEKD+NL+++LYLRDYLQQAGA+V+MTREGDYDLA + TKGY+K Sbjct: 61 AGHGGPDGGAVSRQGVIEKDVNLAITLYLRDYLQQAGALVIMTREGDYDLAAESTKGYSK 120 Query: 121 RKTEDLKQRVRSIEEKRADLFVSVHMNSVPSGRWSGAQTFYYPNHTGNKALAGFVQAELK 180 RKTEDLKQRVR IE+ +ADLF+S+H+NS+PS RW GAQTF+ PN+ +K LA +QAE++ Sbjct: 121 RKTEDLKQRVRMIEDSKADLFISMHLNSIPSNRWRGAQTFFPPNNEDSKKLAELIQAEIR 180 Query: 181 ATLENTDRIAKTVNTVYLLKALKMPAVLVEVGFLSHPQESALLGDDVYQRKVATSIYRGI 240 LENT R+AKT + +YLL+AL+MP+ LVEVGFLSHPQES LL D+ YQRKVA S+Y GI Sbjct: 181 HNLENTQRLAKTDDKIYLLRALRMPSALVEVGFLSHPQESELLRDEKYQRKVAASVYNGI 240 Query: 241 LRY 243 LRY Sbjct: 241 LRY 243 >B1DJT8.1 B1DJT8_9BACL N-acetylmuramoyl-L-alanine amidase CwlD Length = 269 Score = 291 bits (746), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 147/247 (59%), Positives = 189/247 (76%), Gaps = 8/247 (3%) Query: 5 KQGKVSVWISWSS---IKKGMLGAVLLAVMIGIIAYDMPTAKTLNYWSLPLSGKVIAIDA 61 ++ ++ VW+++ I GM A L+ I + P ++T +YW+LPLSGK IAIDA Sbjct: 2 RRKRLVVWMTYKGALRIAAGM--AALVLTWIILTQGGEPVSRTWSYWTLPLSGKTIAIDA 59 Query: 62 GHGGPDGGAVSREGLIEKDINLSVSLYLRDYLQQAGAIVVMTREGDYDLARQDTKGYAKR 121 GHGG DGGAVS+EG++EKD+NL+++LYLRDYLQQAGAIV++TREGDYDLA QDTKGY+KR Sbjct: 60 GHGGVDGGAVSKEGVVEKDLNLAIALYLRDYLQQAGAIVLLTREGDYDLAGQDTKGYSKR 119 Query: 122 KTEDLKQRVRSIEEKRADLFVSVHMNSVPSGRWSGAQTFYYP-NHTGNKALAGFVQAELK 180 KTEDL QRV +I+E + L +S+HMNS+PS +WSGAQTF+ P NH N+ LA F+Q E++ Sbjct: 120 KTEDLLQRVANIKESKPSLVISIHMNSIPSAKWSGAQTFFNPANHPDNQVLATFIQNEIR 179 Query: 181 ATLENTDRIA-KTVNTVYLLKAL-KMPAVLVEVGFLSHPQESALLGDDVYQRKVATSIYR 238 LENT R+A N VYLLKAL +P LVEVGFLS+P E+A L D YQR+VA SIY+ Sbjct: 180 RNLENTSRVAVMDKNNVYLLKALDDIPTALVEVGFLSNPGEAARLADSNYQRQVAASIYQ 239 Query: 239 GILRYAS 245 GILRY+S Sbjct: 240 GILRYSS 246 >C3BER6.1 C3BER6_9BACI Germination-specific N-acetylmuramoyl-L-alanine amidase Length = 228 Score = 233 bits (595), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 123/226 (54%), Positives = 164/226 (72%), Gaps = 3/226 (1%) Query: 22 MLGAVLLAVMIGIIAYDMPTAKTLNYWSLPLSGKVIAIDAGHGGPDGGAVSREGLIEKDI 81 ML AV V+ ++ + AK+ W+LPLSGKVI +DAGHGGPDGGAV + ++EKDI Sbjct: 1 MLAAV---VLFFLVKQEFQIAKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKDI 57 Query: 82 NLSVSLYLRDYLQQAGAIVVMTREGDYDLARQDTKGYAKRKTEDLKQRVRSIEEKRADLF 141 L ++ ++DYLQ+ GA+V++TREGDYDLA +DTK Y++RK EDLK+RV I + D F Sbjct: 58 TLEITKMVQDYLQEQGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVEIINKPDVDFF 117 Query: 142 VSVHMNSVPSGRWSGAQTFYYPNHTGNKALAGFVQAELKATLENTDRIAKTVNTVYLLKA 201 VSVH+N++PS GAQTFYY + N+ A F+QAEL+ +LENT+R AKT++ VYLLK Sbjct: 118 VSVHLNALPSSSSKGAQTFYYRSLIENERAAKFIQAELRTSLENTNRSAKTISRVYLLKY 177 Query: 202 LKMPAVLVEVGFLSHPQESALLGDDVYQRKVATSIYRGILRYASEQ 247 K P LVE GFLS+ E +L + YQ+KVA +IYRGILRY +E+ Sbjct: 178 AKTPGALVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEK 223 >C3AY75.1 C3AY75_BACMY Germination-specific N-acetylmuramoyl-L-alanine amidase Length = 228 Score = 232 bits (591), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 122/226 (53%), Positives = 163/226 (72%), Gaps = 3/226 (1%) Query: 22 MLGAVLLAVMIGIIAYDMPTAKTLNYWSLPLSGKVIAIDAGHGGPDGGAVSREGLIEKDI 81 ML AV V+ ++ + K+ W+LPLSGKVI +DAGHGGPDGGAV + ++EKDI Sbjct: 1 MLAAV---VLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKDI 57 Query: 82 NLSVSLYLRDYLQQAGAIVVMTREGDYDLARQDTKGYAKRKTEDLKQRVRSIEEKRADLF 141 L ++ ++DYLQ+ GA+V++TREGDYDLA +DTK Y++RK EDLK+RV I + D F Sbjct: 58 TLEITKMVQDYLQEQGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVEIINKPDVDFF 117 Query: 142 VSVHMNSVPSGRWSGAQTFYYPNHTGNKALAGFVQAELKATLENTDRIAKTVNTVYLLKA 201 VSVH+N++PS GAQTFYY + N+ A F+QAEL+ +LENT+R AKT++ VYLLK Sbjct: 118 VSVHLNALPSSSSKGAQTFYYRSLIENERAAKFIQAELRTSLENTNRSAKTISRVYLLKY 177 Query: 202 LKMPAVLVEVGFLSHPQESALLGDDVYQRKVATSIYRGILRYASEQ 247 K P LVE GFLS+ E +L + YQ+KVA +IYRGILRY +E+ Sbjct: 178 AKTPGALVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEK 223 >C3AGE3.1 C3AGE3_BACMY Germination-specific N-acetylmuramoyl-L-alanine amidase Length = 228 Score = 232 bits (591), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 122/226 (53%), Positives = 163/226 (72%), Gaps = 3/226 (1%) Query: 22 MLGAVLLAVMIGIIAYDMPTAKTLNYWSLPLSGKVIAIDAGHGGPDGGAVSREGLIEKDI 81 ML AV V+ ++ + K+ W+LPLSGKVI +DAGHGGPDGGAV + ++EKDI Sbjct: 1 MLAAV---VLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIVEKDI 57 Query: 82 NLSVSLYLRDYLQQAGAIVVMTREGDYDLARQDTKGYAKRKTEDLKQRVRSIEEKRADLF 141 L ++ ++DYLQ+ GA+V++TREGDYDLA +DTK Y++RK EDLK+RV I + D F Sbjct: 58 TLEITKMVQDYLQEQGALVILTREGDYDLADKDTKSYSRRKAEDLKKRVEIINKPDVDFF 117 Query: 142 VSVHMNSVPSGRWSGAQTFYYPNHTGNKALAGFVQAELKATLENTDRIAKTVNTVYLLKA 201 VSVH+N++PS GAQTFYY + N+ A F+QAEL+ +LENT+R AKT++ VYLLK Sbjct: 118 VSVHLNALPSSSSKGAQTFYYRSLIENERAAKFIQAELRTSLENTNRSAKTISRVYLLKY 177 Query: 202 LKMPAVLVEVGFLSHPQESALLGDDVYQRKVATSIYRGILRYASEQ 247 K P LVE GFLS+ E +L + YQ+KVA +IYRGILRY +E+ Sbjct: 178 AKTPGALVEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEK 223 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,488,405,010 Number of extensions: 59943484 Number of successful extensions: 220084 Number of sequences better than 1.0e-05: 1854 Number of HSP's gapped: 217562 Number of HSP's successfully gapped: 1864 Length of query: 248 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 116 Effective length of database: 1,817,122,223 Effective search space: 210786177868 Effective search space used: 210786177868 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)